YMR061W / RNA14

Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping

Zygosity: Heterozygous strain
fixedexpanded
Profile for YMR061W / RNA14

Click on Significant Values for Screen Details ID:SGTC_54|Compound:0159-0055|FD-Score:3.36|P-value:8.21E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:3.67|P-value:2.97E-4 ID:SGTC_125|Compound:0133-0007|FD-Score:3.63|P-value:3.36E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:3.65|P-value:3.14E-4 ID:SGTC_259|Compound:usnic acid|FD-Score:4.02|P-value:8.17E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.57|P-value:4.17E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.10|P-value:6.00E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.66|P-value:6.39E-6 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.60|P-value:3.76E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:3.50|P-value:5.28E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.72|P-value:2.44E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:4.77|P-value:3.94E-6 ID:SGTC_1037|Compound:k284-1091|FD-Score:4.52|P-value:1.14E-5 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.74|P-value:2.30E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:-3.59|P-value:3.87E-4 ID:SGTC_1471|Compound:k673-0312|FD-Score:3.55|P-value:4.44E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:6.05|P-value:7.07E-9 ID:SGTC_1732|Compound:st034308|FD-Score:3.99|P-value:9.16E-5 ID:SGTC_1745|Compound:st081495|FD-Score:5.06|P-value:1.08E-6 ID:SGTC_1754|Compound:st042065|FD-Score:5.15|P-value:7.15E-7 ID:SGTC_1778|Compound:st046819|FD-Score:3.78|P-value:1.99E-4 ID:SGTC_2012|Compound:4023501|FD-Score:-3.71|P-value:2.58E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.73|P-value:2.33E-4 ID:SGTC_2036|Compound:5160441|FD-Score:5.82|P-value:2.45E-8 ID:SGTC_2265|Compound:7929201|FD-Score:3.45|P-value:6.21E-4 ID:SGTC_2270|Compound:7924575|FD-Score:3.47|P-value:5.76E-4 ID:SGTC_2421|Compound:5690543|FD-Score:-4.24|P-value:3.61E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-3.55|P-value:4.45E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:3.45|P-value:6.10E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.35|P-value:8.58E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:9.18|P-value:3.89E-18 ID:SGTC_2819|Compound:7996655|FD-Score:-10.40|P-value:1.47E-22 ID:SGTC_2822|Compound:7994718|FD-Score:4.78|P-value:3.80E-6 ID:SGTC_2828|Compound:7998861|FD-Score:-3.98|P-value:9.61E-5 ID:SGTC_3108|Compound:9121785|FD-Score:4.63|P-value:7.32E-6 ID:SGTC_3111|Compound:9122088|FD-Score:-3.88|P-value:1.38E-4 ID:SGTC_3113|Compound:9123498|FD-Score:3.62|P-value:3.51E-4 ID:SGTC_3118|Compound:9124250|FD-Score:3.41|P-value:6.97E-4 ID:SGTC_3262|Compound:9137218|FD-Score:-3.35|P-value:8.42E-4 ID:SGTC_54|Compound:0159-0055|FD-Score:3.36|P-value:8.21E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:3.67|P-value:2.97E-4 ID:SGTC_125|Compound:0133-0007|FD-Score:3.63|P-value:3.36E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:3.65|P-value:3.14E-4 ID:SGTC_259|Compound:usnic acid|FD-Score:4.02|P-value:8.17E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.57|P-value:4.17E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.10|P-value:6.00E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.66|P-value:6.39E-6 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.60|P-value:3.76E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:3.50|P-value:5.28E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.72|P-value:2.44E-4 ID:SGTC_948|Compound:3448-0295|FD-Score:4.77|P-value:3.94E-6 ID:SGTC_1037|Compound:k284-1091|FD-Score:4.52|P-value:1.14E-5 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.74|P-value:2.30E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:-3.59|P-value:3.87E-4 ID:SGTC_1471|Compound:k673-0312|FD-Score:3.55|P-value:4.44E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:6.05|P-value:7.07E-9 ID:SGTC_1732|Compound:st034308|FD-Score:3.99|P-value:9.16E-5 ID:SGTC_1745|Compound:st081495|FD-Score:5.06|P-value:1.08E-6 ID:SGTC_1754|Compound:st042065|FD-Score:5.15|P-value:7.15E-7 ID:SGTC_1778|Compound:st046819|FD-Score:3.78|P-value:1.99E-4 ID:SGTC_2012|Compound:4023501|FD-Score:-3.71|P-value:2.58E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.73|P-value:2.33E-4 ID:SGTC_2036|Compound:5160441|FD-Score:5.82|P-value:2.45E-8 ID:SGTC_2265|Compound:7929201|FD-Score:3.45|P-value:6.21E-4 ID:SGTC_2270|Compound:7924575|FD-Score:3.47|P-value:5.76E-4 ID:SGTC_2421|Compound:5690543|FD-Score:-4.24|P-value:3.61E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-3.55|P-value:4.45E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:3.45|P-value:6.10E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.35|P-value:8.58E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:9.18|P-value:3.89E-18 ID:SGTC_2819|Compound:7996655|FD-Score:-10.40|P-value:1.47E-22 ID:SGTC_2822|Compound:7994718|FD-Score:4.78|P-value:3.80E-6 ID:SGTC_2828|Compound:7998861|FD-Score:-3.98|P-value:9.61E-5 ID:SGTC_3108|Compound:9121785|FD-Score:4.63|P-value:7.32E-6 ID:SGTC_3111|Compound:9122088|FD-Score:-3.88|P-value:1.38E-4 ID:SGTC_3113|Compound:9123498|FD-Score:3.62|P-value:3.51E-4 ID:SGTC_3118|Compound:9124250|FD-Score:3.41|P-value:6.97E-4 ID:SGTC_3262|Compound:9137218|FD-Score:-3.35|P-value:8.42E-4

Top fitness defect scores for YMR061W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2756 carbadox 9.18 75.1 µM 3.89E-18 2 2.11E-20 0.20
2 SGTC_1597 7-benzyloxygramine 6.05 47.5 µM 7.07E-9 calcium & mitochondrial duress 34 7.09E-10 0.09
3 SGTC_2036 5160441 5.82 105.0 µM 2.45E-8 NEO1 13 2.90E-9 0.56
4 SGTC_1754 st042065 5.15 16.1 µM 7.15E-7 NEO1-PIK1 18 1.34E-7 0.29
5 SGTC_1745 st081495 5.06 74.0 µM 1.08E-6 RNA processing & uracil transport 17 2.13E-7 0.03
6 SGTC_2822 7994718 4.78 71.4 µM 3.80E-6 4 8.89E-7 0.70
7 SGTC_948 3448-0295 4.77 75.3 µM 3.94E-6 5 9.25E-7 0.04
8 SGTC_3108 9121785 4.63 49.5 µM 7.32E-6 5 1.87E-6 0.17
9 SGTC_1037 k284-1091 4.52 78.7 µM 1.14E-5 4 3.08E-6 0.91
10 SGTC_351 caspofungin 4.10 25.0 nM 6.00E-5 cell wall 30 2.03E-5 0.07
11 SGTC_259 usnic acid 4.02 108.9 µM 8.17E-5 6 2.87E-5 0.24
12 SGTC_1732 st034308 3.99 51.8 µM 9.16E-5 8 3.27E-5 0.01
13 SGTC_1778 st046819 3.78 58.9 µM 1.99E-4 6 7.86E-5 0.21
14 SGTC_1140 3229-1848 3.74 3.0 µM 2.30E-4 7 9.24E-5 0.12
15 SGTC_2021 4019055 3.73 173.0 µM 2.33E-4 endomembrane recycling 38 9.41E-5 0.03
16 SGTC_682 0kpi-0099 3.72 17.0 µM 2.44E-4 33 9.91E-5 0.00
17 SGTC_71 0417-1750 3.67 47.8 µM 2.97E-4 Golgi 10 1.24E-4 0.04
18 SGTC_126 0417-1624 3.65 672.5 µM 3.14E-4 DNA intercalators 20 1.32E-4 0.10
19 SGTC_125 0133-0007 3.63 11.8 µM 3.36E-4 DNA intercalators 7 1.42E-4 0.01
20 SGTC_3113 9123498 3.62 49.5 µM 3.51E-4 9 1.49E-4 0.12

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.154 3.80E-19 YGR253C PUP2 het Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.133 1.15E-14 YLR330W CHS5 hom Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane
0.132 1.96E-14 YJR093C FIP1 het Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p
0.130 5.33E-14 YDR228C PCF11 het mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
0.119 4.72E-12 YGL210W YPT32 hom Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication
0.113 4.54E-11 YCL021W-A_p YCL021W-A_p hom Putative protein of unknown function
0.113 5.75E-11 YPR070W MED1 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.111 9.81E-11 YDR239C YDR239C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments
0.107 5.73E-10 YGL035C MIG1 hom Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion
0.106 7.00E-10 YPL032C SVL3 hom Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.105 9.36E-10 YBL082C ALG3 hom Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.103 2.22E-9 YJL129C TRK1 hom Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
0.103 2.28E-9 YER133W GLC7 het Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
0.102 3.38E-9 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.102 3.35E-9 YBL054W TOD6 hom PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication