HIPHOP chemogenomics database

YMR080C / NAM7

ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress

Zygosity: Homozygous strain

fixedexpanded
Profile for YMR080C / NAM7

Click on Significant Values for Screen Details

Top fitness defect scores for YMR080C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2494	18-aminoabieta-8,11,13-triene sulfate	5.05	3.7 µM	1.35E-6		32	2.21E-7
2	SGTC_1628	st004252	5.01	77.7 µM	1.59E-6		7	2.67E-7
3	SGTC_76	0433-0009	4.77	400.3 µM	4.75E-6		3	9.35E-7
4	SGTC_71	0417-1750	4.25	47.8 µM	3.95E-5	Golgi	35	1.07E-5
5	SGTC_909	3448-9333	4.24	149.0 µM	4.04E-5		15	1.10E-5
6	SGTC_2081	5214970	4.21	32.3 µM	4.53E-5		21	1.25E-5
7	SGTC_1046	2001-0062	4.01	216.0 µM	9.81E-5		16	3.04E-5
8	SGTC_2695	st077686	3.92	52.0 µM	1.35E-4		37	4.39E-5
9	SGTC_1065	prochlorperazine maleate	3.92	2.5 µM	1.37E-4		13	4.44E-5
10	SGTC_2072	5231554	3.65	129.0 µM	3.49E-4		13	1.30E-4
11	SGTC_6	0844-0013	3.62	19.8 µM	3.88E-4		124	1.47E-4
12	SGTC_374	0880-0231	3.59	82.3 µM	4.32E-4	iron homeostasis	31	1.67E-4
13	SGTC_2762	leflunomide	3.56	42.1 µM	4.74E-4		45	1.85E-4
14	SGTC_2079	5212404	3.41	121.0 µM	7.68E-4		22	3.22E-4
15	SGTC_2450	5739737	3.35	12.9 µM	9.23E-4		45	3.98E-4
16	SGTC_1904	st060225	3.32	78.0 µM	0.00103		38	4.50E-4
17	SGTC_2485	5633444	3.29	17.8 µM	0.00113	redox potentiating	72	5.02E-4
18	SGTC_1438	4092-1045	3.22	4.0 µM	0.00138		24	6.31E-4
19	SGTC_495	decylubiquinone	3.19	155.0 µM	0.00154	copper-dependent oxidative stress	63	7.13E-4
20	SGTC_3005	9076496	3.19	71.4 µM	0.00155		21	7.18E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.234	8.57E-43	YDR452W	PPN1	hom	Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress
0.193	1.84E-29	YPL004C	LSP1	hom	Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family
0.153	4.64E-19	YBR176W	ECM31	hom	Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate
0.153	6.41E-19	YDR206W	EBS1	hom	Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors
0.144	5.96E-17	YDR165W	TRM82	hom	Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.144	6.61E-17	YMR191W	SPG5	hom	Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
0.140	3.18E-16	YOR214C_p	YOR214C_p	hom	Putative protein of unknown function; YOR214C is not an essential gene
0.140	4.91E-16	YGL004C	RPN14	hom	Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p
0.139	5.63E-16	YKL215C	OXP1	hom	5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
0.136	2.37E-15	YPR022C_p	YPR022C_p	hom	Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
0.136	2.91E-15	YLR354C	TAL1	hom	Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate
0.136	3.07E-15	YHR021W-A_p	ECM12_p	hom	Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.132	1.68E-14	YNL334C	SNO2	hom	Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin
0.127	1.39E-13	YDR266C	HEL2	hom	RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor
0.123	1.01E-12	YOL061W	PRS5	hom	5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress