YMR086W / SEG1

Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR086W / SEG1

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:4.09|P-value:1.65E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.10|P-value:8.20E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:-3.12|P-value:7.82E-4 ID:SGTC_128|Compound:0906-2981|FD-Score:4.52|P-value:2.17E-6 ID:SGTC_235|Compound:0330-0087|FD-Score:3.70|P-value:8.58E-5 ID:SGTC_270|Compound:tamoxifen|FD-Score:-4.64|P-value:1.25E-6 ID:SGTC_440|Compound:fluspirilene|FD-Score:3.44|P-value:2.37E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:3.41|P-value:2.67E-4 ID:SGTC_648|Compound:0375-0473|FD-Score:3.11|P-value:7.95E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:-3.19|P-value:6.00E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:3.31|P-value:3.80E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:3.37|P-value:3.11E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:4.38|P-value:4.29E-6 ID:SGTC_996|Compound:3970-0799|FD-Score:3.49|P-value:1.97E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:3.32|P-value:3.67E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:4.82|P-value:4.90E-7 ID:SGTC_1005|Compound:4092-0690|FD-Score:-4.13|P-value:1.37E-5 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.91|P-value:3.53E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.98|P-value:2.66E-5 ID:SGTC_1212|Compound:0520-0165|FD-Score:-3.21|P-value:5.64E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:-3.13|P-value:7.58E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:4.41|P-value:3.72E-6 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.11|P-value:7.79E-4 ID:SGTC_1503|Compound:4585-0012|FD-Score:-3.96|P-value:2.95E-5 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-3.69|P-value:9.09E-5 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.06|P-value:9.44E-4 ID:SGTC_1542|Compound:7-hydroxy-3'-methoxyflavone|FD-Score:3.69|P-value:8.95E-5 ID:SGTC_1695|Compound:st026914|FD-Score:3.19|P-value:5.86E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:4.29|P-value:6.65E-6 ID:SGTC_1897|Compound:st060201|FD-Score:3.78|P-value:6.23E-5 ID:SGTC_1907|Compound:st060210|FD-Score:3.41|P-value:2.66E-4 ID:SGTC_1915|Compound:st067105|FD-Score:4.36|P-value:4.76E-6 ID:SGTC_1917|Compound:st066507|FD-Score:-3.05|P-value:9.94E-4 ID:SGTC_1942|Compound:st076330|FD-Score:4.31|P-value:6.01E-6 ID:SGTC_1954|Compound:st077032|FD-Score:3.49|P-value:1.97E-4 ID:SGTC_2011|Compound:4012611|FD-Score:3.07|P-value:9.00E-4 ID:SGTC_2065|Compound:5221596|FD-Score:-3.07|P-value:9.18E-4 ID:SGTC_2285|Compound:7945142|FD-Score:5.92|P-value:8.99E-10 ID:SGTC_2326|Compound:6164455|FD-Score:-3.65|P-value:1.07E-4 ID:SGTC_2332|Compound:7982454|FD-Score:3.12|P-value:7.69E-4 ID:SGTC_2380|Compound:9035439|FD-Score:3.76|P-value:6.56E-5 ID:SGTC_2401|Compound:5128683|FD-Score:3.67|P-value:9.48E-5 ID:SGTC_2700|Compound:st077718|FD-Score:-3.30|P-value:4.01E-4 ID:SGTC_2710|Compound:st078562|FD-Score:-3.26|P-value:4.72E-4 ID:SGTC_2853|Compound:9015042|FD-Score:-3.43|P-value:2.54E-4 ID:SGTC_2856|Compound:9022925|FD-Score:4.74|P-value:7.32E-7 ID:SGTC_2896|Compound:9052656|FD-Score:5.08|P-value:1.21E-7 ID:SGTC_2963|Compound:9082507|FD-Score:-3.42|P-value:2.64E-4 ID:SGTC_2996|Compound:9066192|FD-Score:4.28|P-value:6.75E-6 ID:SGTC_3054|Compound:9094397|FD-Score:-3.19|P-value:6.03E-4 ID:SGTC_3254|Compound:9136651|FD-Score:3.23|P-value:5.22E-4 ID:SGTC_3341|Compound:9147222|FD-Score:3.10|P-value:8.11E-4 ID:SGTC_10|Compound:0279-0212|FD-Score:4.09|P-value:1.65E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.10|P-value:8.20E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:-3.12|P-value:7.82E-4 ID:SGTC_128|Compound:0906-2981|FD-Score:4.52|P-value:2.17E-6 ID:SGTC_235|Compound:0330-0087|FD-Score:3.70|P-value:8.58E-5 ID:SGTC_270|Compound:tamoxifen|FD-Score:-4.64|P-value:1.25E-6 ID:SGTC_440|Compound:fluspirilene|FD-Score:3.44|P-value:2.37E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:3.41|P-value:2.67E-4 ID:SGTC_648|Compound:0375-0473|FD-Score:3.11|P-value:7.95E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:-3.19|P-value:6.00E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:3.31|P-value:3.80E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:3.37|P-value:3.11E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:4.38|P-value:4.29E-6 ID:SGTC_996|Compound:3970-0799|FD-Score:3.49|P-value:1.97E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:3.32|P-value:3.67E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:4.82|P-value:4.90E-7 ID:SGTC_1005|Compound:4092-0690|FD-Score:-4.13|P-value:1.37E-5 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.91|P-value:3.53E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.98|P-value:2.66E-5 ID:SGTC_1212|Compound:0520-0165|FD-Score:-3.21|P-value:5.64E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:-3.13|P-value:7.58E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:4.41|P-value:3.72E-6 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.11|P-value:7.79E-4 ID:SGTC_1503|Compound:4585-0012|FD-Score:-3.96|P-value:2.95E-5 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-3.69|P-value:9.09E-5 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.06|P-value:9.44E-4 ID:SGTC_1542|Compound:7-hydroxy-3'-methoxyflavone|FD-Score:3.69|P-value:8.95E-5 ID:SGTC_1695|Compound:st026914|FD-Score:3.19|P-value:5.86E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:4.29|P-value:6.65E-6 ID:SGTC_1897|Compound:st060201|FD-Score:3.78|P-value:6.23E-5 ID:SGTC_1907|Compound:st060210|FD-Score:3.41|P-value:2.66E-4 ID:SGTC_1915|Compound:st067105|FD-Score:4.36|P-value:4.76E-6 ID:SGTC_1917|Compound:st066507|FD-Score:-3.05|P-value:9.94E-4 ID:SGTC_1942|Compound:st076330|FD-Score:4.31|P-value:6.01E-6 ID:SGTC_1954|Compound:st077032|FD-Score:3.49|P-value:1.97E-4 ID:SGTC_2011|Compound:4012611|FD-Score:3.07|P-value:9.00E-4 ID:SGTC_2065|Compound:5221596|FD-Score:-3.07|P-value:9.18E-4 ID:SGTC_2285|Compound:7945142|FD-Score:5.92|P-value:8.99E-10 ID:SGTC_2326|Compound:6164455|FD-Score:-3.65|P-value:1.07E-4 ID:SGTC_2332|Compound:7982454|FD-Score:3.12|P-value:7.69E-4 ID:SGTC_2380|Compound:9035439|FD-Score:3.76|P-value:6.56E-5 ID:SGTC_2401|Compound:5128683|FD-Score:3.67|P-value:9.48E-5 ID:SGTC_2700|Compound:st077718|FD-Score:-3.30|P-value:4.01E-4 ID:SGTC_2710|Compound:st078562|FD-Score:-3.26|P-value:4.72E-4 ID:SGTC_2853|Compound:9015042|FD-Score:-3.43|P-value:2.54E-4 ID:SGTC_2856|Compound:9022925|FD-Score:4.74|P-value:7.32E-7 ID:SGTC_2896|Compound:9052656|FD-Score:5.08|P-value:1.21E-7 ID:SGTC_2963|Compound:9082507|FD-Score:-3.42|P-value:2.64E-4 ID:SGTC_2996|Compound:9066192|FD-Score:4.28|P-value:6.75E-6 ID:SGTC_3054|Compound:9094397|FD-Score:-3.19|P-value:6.03E-4 ID:SGTC_3254|Compound:9136651|FD-Score:3.23|P-value:5.22E-4 ID:SGTC_3341|Compound:9147222|FD-Score:3.10|P-value:8.11E-4

Top fitness defect scores for YMR086W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2285 7945142 5.92 200.0 µM 8.99E-10 3 1.61E-9
2 SGTC_2896 9052656 5.08 71.4 µM 1.21E-7 endomembrane recycling 60 1.87E-7
3 SGTC_1003 4049-0218 4.82 100.0 µM 4.90E-7 9 7.25E-7
4 SGTC_2856 9022925 4.74 19.5 µM 7.32E-7 10 1.07E-6
5 SGTC_128 0906-2981 4.52 13.4 µM 2.17E-6 8 3.08E-6
6 SGTC_1457 k015-0032 4.41 57.3 µM 3.72E-6 26 5.19E-6
7 SGTC_934 2950-0684 4.38 338.0 µM 4.29E-6 14 5.97E-6
8 SGTC_1915 st067105 4.36 30.6 µM 4.76E-6 RNA pol III & RNase P/MRP 31 6.59E-6
9 SGTC_1942 st076330 4.31 65.2 µM 6.01E-6 21 8.26E-6
10 SGTC_1787 fluconazole 4.29 80.0 µM 6.65E-6 iron homeostasis 20 9.12E-6
11 SGTC_2996 9066192 4.28 71.4 µM 6.75E-6 26 9.26E-6
12 SGTC_10 0279-0212 4.09 42.6 µM 1.65E-5 TRP & mitochondrial translation 112 2.20E-5
13 SGTC_1043 0916-0009 3.98 19.3 µM 2.66E-5 23 3.50E-5
14 SGTC_1032 k061-1018 3.91 98.7 µM 3.53E-5 23 4.61E-5
15 SGTC_1897 st060201 3.78 15.0 µM 6.23E-5 mitochondrial processes 22 7.99E-5
16 SGTC_2380 9035439 3.76 200.0 µM 6.56E-5 endomembrane recycling 29 8.40E-5
17 SGTC_235 0330-0087 3.70 5.0 mM 8.58E-5 TSC3-RPN4 34 1.09E-4
18 SGTC_1542 7-hydroxy-3'-methoxyflavone 3.69 49.7 µM 8.95E-5 18 1.14E-4
19 SGTC_2401 5128683 3.67 200.0 µM 9.48E-5 50 1.20E-4
20 SGTC_996 3970-0799 3.49 1.8 µM 1.97E-4 115 2.45E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.132 1.47E-14 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.097 1.78E-8 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.097 1.87E-8 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.095 4.05E-8 YFR045W_p YFR045W_p hom Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white
0.092 8.48E-8 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.092 9.67E-8 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.091 1.46E-7 YJL092W SRS2 hom DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.090 1.73E-7 YNR033W ABZ1 hom Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress
0.090 1.99E-7 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.089 2.28E-7 YOL082W ATG19 hom Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles
0.087 4.29E-7 YKL071W_p YKL071W_p hom Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.086 5.52E-7 YPR028W YOP1 hom Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress
0.081 2.59E-6 YBR042C CST26 hom Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication
0.078 5.99E-6 YLR079W SIC1 hom Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1
0.076 9.39E-6 YMR240C CUS1 het Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p