YMR113W / FOL3

Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis

Zygosity: Heterozygous strain
fixedexpanded
Profile for YMR113W / FOL3

Click on Significant Values for Screen Details ID:SGTC_63|Compound:0099-0242|FD-Score:10.10|P-value:9.55E-24 ID:SGTC_69|Compound:0096-0274|FD-Score:7.11|P-value:1.28E-12 ID:SGTC_70|Compound:0141-0289|FD-Score:3.56|P-value:2.18E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:-3.71|P-value:1.52E-4 ID:SGTC_104|Compound:0074-0074|FD-Score:3.24|P-value:6.84E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:5.80|P-value:5.47E-9 ID:SGTC_123|Compound:0083-0120|FD-Score:3.51|P-value:2.63E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:6.39|P-value:1.54E-10 ID:SGTC_177|Compound:1327-0068|FD-Score:4.24|P-value:1.40E-5 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.68|P-value:1.37E-4 ID:SGTC_264|Compound:promethazine|FD-Score:4.73|P-value:1.55E-6 ID:SGTC_266|Compound:formosanin C|FD-Score:-3.34|P-value:5.78E-4 ID:SGTC_441|Compound:propafenone|FD-Score:-3.19|P-value:9.56E-4 ID:SGTC_446|Compound:go6976|FD-Score:3.41|P-value:3.78E-4 ID:SGTC_597|Compound:k018-0003|FD-Score:3.79|P-value:9.09E-5 ID:SGTC_601|Compound:0986-0029|FD-Score:5.48|P-value:3.28E-8 ID:SGTC_619|Compound:1652-1634|FD-Score:3.13|P-value:9.95E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:3.19|P-value:7.91E-4 ID:SGTC_636|Compound:0kpi-0074|FD-Score:3.53|P-value:2.40E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:-3.63|P-value:2.03E-4 ID:SGTC_730|Compound:1436-0052|FD-Score:14.40|P-value:1.04E-45 ID:SGTC_868|Compound:0527-0199|FD-Score:-3.34|P-value:5.78E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:3.15|P-value:9.22E-4 ID:SGTC_903|Compound:1134-0042|FD-Score:3.40|P-value:3.92E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:-3.18|P-value:9.82E-4 ID:SGTC_991|Compound:3996-0057|FD-Score:3.33|P-value:4.96E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:6.85|P-value:7.66E-12 ID:SGTC_1132|Compound:3456-3614|FD-Score:-3.56|P-value:2.64E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.31|P-value:6.30E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:-5.01|P-value:5.11E-7 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.48|P-value:2.89E-4 ID:SGTC_1609|Compound:st001574|FD-Score:-3.49|P-value:3.38E-4 ID:SGTC_1642|Compound:st009582|FD-Score:4.16|P-value:2.00E-5 ID:SGTC_1717|Compound:st033143|FD-Score:3.14|P-value:9.61E-4 ID:SGTC_1774|Compound:st048371|FD-Score:-4.05|P-value:3.90E-5 ID:SGTC_1798|Compound:st045719|FD-Score:3.21|P-value:7.61E-4 ID:SGTC_1843|Compound:st055809|FD-Score:3.45|P-value:3.26E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.70|P-value:1.81E-6 ID:SGTC_2052|Compound:5265182|FD-Score:3.21|P-value:7.59E-4 ID:SGTC_2060|Compound:5238662|FD-Score:3.18|P-value:8.24E-4 ID:SGTC_2080|Compound:5212524|FD-Score:3.46|P-value:3.12E-4 ID:SGTC_2086|Compound:5274770|FD-Score:4.81|P-value:1.04E-6 ID:SGTC_2126|Compound:5251174|FD-Score:-4.26|P-value:1.66E-5 ID:SGTC_2134|Compound:5324836|FD-Score:-3.91|P-value:6.98E-5 ID:SGTC_2139|Compound:5578692|FD-Score:17.60|P-value:2.14E-67 ID:SGTC_2164|Compound:5685496|FD-Score:-5.65|P-value:1.75E-8 ID:SGTC_2399|Compound:5884133|FD-Score:-3.36|P-value:5.31E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:7.25|P-value:4.80E-13 ID:SGTC_2459|Compound:5811157|FD-Score:14.70|P-value:7.93E-48 ID:SGTC_2471|Compound:5466956|FD-Score:18.90|P-value:4.69E-77 ID:SGTC_2475|Compound:5812728|FD-Score:20.30|P-value:4.53E-89 ID:SGTC_2481|Compound:5478948|FD-Score:5.83|P-value:4.68E-9 ID:SGTC_2482|Compound:5981709|FD-Score:3.79|P-value:9.01E-5 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:3.77|P-value:9.99E-5 ID:SGTC_2779|Compound:methotrexate|FD-Score:4.64|P-value:2.31E-6 ID:SGTC_2967|Compound:9089538|FD-Score:4.15|P-value:2.11E-5 ID:SGTC_3067|Compound:9117431|FD-Score:-3.78|P-value:1.17E-4 ID:SGTC_3114|Compound:9122766|FD-Score:3.21|P-value:7.53E-4 ID:SGTC_3154|Compound:9100113|FD-Score:3.17|P-value:8.64E-4 ID:SGTC_3234|Compound:9132280|FD-Score:3.22|P-value:7.29E-4 ID:SGTC_63|Compound:0099-0242|FD-Score:10.10|P-value:9.55E-24 ID:SGTC_69|Compound:0096-0274|FD-Score:7.11|P-value:1.28E-12 ID:SGTC_70|Compound:0141-0289|FD-Score:3.56|P-value:2.18E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:-3.71|P-value:1.52E-4 ID:SGTC_104|Compound:0074-0074|FD-Score:3.24|P-value:6.84E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:5.80|P-value:5.47E-9 ID:SGTC_123|Compound:0083-0120|FD-Score:3.51|P-value:2.63E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:6.39|P-value:1.54E-10 ID:SGTC_177|Compound:1327-0068|FD-Score:4.24|P-value:1.40E-5 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.68|P-value:1.37E-4 ID:SGTC_264|Compound:promethazine|FD-Score:4.73|P-value:1.55E-6 ID:SGTC_266|Compound:formosanin C|FD-Score:-3.34|P-value:5.78E-4 ID:SGTC_441|Compound:propafenone|FD-Score:-3.19|P-value:9.56E-4 ID:SGTC_446|Compound:go6976|FD-Score:3.41|P-value:3.78E-4 ID:SGTC_597|Compound:k018-0003|FD-Score:3.79|P-value:9.09E-5 ID:SGTC_601|Compound:0986-0029|FD-Score:5.48|P-value:3.28E-8 ID:SGTC_619|Compound:1652-1634|FD-Score:3.13|P-value:9.95E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:3.19|P-value:7.91E-4 ID:SGTC_636|Compound:0kpi-0074|FD-Score:3.53|P-value:2.40E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:-3.63|P-value:2.03E-4 ID:SGTC_730|Compound:1436-0052|FD-Score:14.40|P-value:1.04E-45 ID:SGTC_868|Compound:0527-0199|FD-Score:-3.34|P-value:5.78E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:3.15|P-value:9.22E-4 ID:SGTC_903|Compound:1134-0042|FD-Score:3.40|P-value:3.92E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:-3.18|P-value:9.82E-4 ID:SGTC_991|Compound:3996-0057|FD-Score:3.33|P-value:4.96E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:6.85|P-value:7.66E-12 ID:SGTC_1132|Compound:3456-3614|FD-Score:-3.56|P-value:2.64E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.31|P-value:6.30E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:-5.01|P-value:5.11E-7 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.48|P-value:2.89E-4 ID:SGTC_1609|Compound:st001574|FD-Score:-3.49|P-value:3.38E-4 ID:SGTC_1642|Compound:st009582|FD-Score:4.16|P-value:2.00E-5 ID:SGTC_1717|Compound:st033143|FD-Score:3.14|P-value:9.61E-4 ID:SGTC_1774|Compound:st048371|FD-Score:-4.05|P-value:3.90E-5 ID:SGTC_1798|Compound:st045719|FD-Score:3.21|P-value:7.61E-4 ID:SGTC_1843|Compound:st055809|FD-Score:3.45|P-value:3.26E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.70|P-value:1.81E-6 ID:SGTC_2052|Compound:5265182|FD-Score:3.21|P-value:7.59E-4 ID:SGTC_2060|Compound:5238662|FD-Score:3.18|P-value:8.24E-4 ID:SGTC_2080|Compound:5212524|FD-Score:3.46|P-value:3.12E-4 ID:SGTC_2086|Compound:5274770|FD-Score:4.81|P-value:1.04E-6 ID:SGTC_2126|Compound:5251174|FD-Score:-4.26|P-value:1.66E-5 ID:SGTC_2134|Compound:5324836|FD-Score:-3.91|P-value:6.98E-5 ID:SGTC_2139|Compound:5578692|FD-Score:17.60|P-value:2.14E-67 ID:SGTC_2164|Compound:5685496|FD-Score:-5.65|P-value:1.75E-8 ID:SGTC_2399|Compound:5884133|FD-Score:-3.36|P-value:5.31E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:7.25|P-value:4.80E-13 ID:SGTC_2459|Compound:5811157|FD-Score:14.70|P-value:7.93E-48 ID:SGTC_2471|Compound:5466956|FD-Score:18.90|P-value:4.69E-77 ID:SGTC_2475|Compound:5812728|FD-Score:20.30|P-value:4.53E-89 ID:SGTC_2481|Compound:5478948|FD-Score:5.83|P-value:4.68E-9 ID:SGTC_2482|Compound:5981709|FD-Score:3.79|P-value:9.01E-5 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:3.77|P-value:9.99E-5 ID:SGTC_2779|Compound:methotrexate|FD-Score:4.64|P-value:2.31E-6 ID:SGTC_2967|Compound:9089538|FD-Score:4.15|P-value:2.11E-5 ID:SGTC_3067|Compound:9117431|FD-Score:-3.78|P-value:1.17E-4 ID:SGTC_3114|Compound:9122766|FD-Score:3.21|P-value:7.53E-4 ID:SGTC_3154|Compound:9100113|FD-Score:3.17|P-value:8.64E-4 ID:SGTC_3234|Compound:9132280|FD-Score:3.22|P-value:7.29E-4

Top fitness defect scores for YMR113W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2475 5812728 20.30 15.0 µM 4.53E-89 mitochondrial processes 2 3.06E-92 6.70
2 SGTC_2471 5466956 18.90 24.8 µM 4.69E-77 mitochondrial processes 1 8.98E-80 4.18
3 SGTC_2139 5578692 17.60 56.5 µM 2.14E-67 superoxide 1 9.45E-70 8.97
4 SGTC_2459 5811157 14.70 9.2 µM 7.93E-48 mitochondrial processes 1 1.88E-49 3.98
5 SGTC_730 1436-0052 14.40 41.2 µM 1.04E-45 mitochondrial processes 3 2.97E-47 6.09
6 SGTC_63 0099-0242 10.10 26.7 µM 9.55E-24 mitochondrial stress 3 1.74E-24 0.42
7 SGTC_2408 sulbentine 7.25 18.4 µM 4.80E-13 12 2.11E-13 0.04
8 SGTC_69 0096-0274 7.11 206.7 µM 1.28E-12 mitochondrial processes 13 5.81E-13 0.47
9 SGTC_1057 3448-5381 6.85 134.0 µM 7.66E-12 calcium & mitochondrial duress 4 3.71E-12 0.61
10 SGTC_173 1273-0061 6.39 2.7 µM 1.54E-10 mitochondrial stress 6 8.26E-11 0.02
11 SGTC_2481 5478948 5.83 45.2 µM 4.68E-9 PDR1 4 2.82E-9 1.11
12 SGTC_119 0121-0043 5.80 24.8 µM 5.47E-9 mitochondrial stress 8 3.31E-9 0.33
13 SGTC_601 0986-0029 5.48 62.9 µM 3.28E-8 calcium & mitochondrial duress 9 2.11E-8 0.05
14 SGTC_2086 5274770 4.81 176.6 µM 1.04E-6 5 7.49E-7 0.15
15 SGTC_264 promethazine 4.73 12.5 mM 1.55E-6 DNA intercalators 16 1.13E-6 0.05
16 SGTC_2021 4019055 4.70 173.0 µM 1.81E-6 endomembrane recycling 28 1.33E-6 0.24
17 SGTC_2779 methotrexate 4.64 400.0 µM 2.31E-6 12 1.71E-6 0.10
18 SGTC_177 1327-0068 4.24 8.4 µM 1.40E-5 19 1.09E-5 0.00
19 SGTC_1642 st009582 4.16 77.7 µM 2.00E-5 RPP1 & pyrimidine depletion 6 1.58E-5 0.07
20 SGTC_2967 9089538 4.15 49.9 µM 2.11E-5 12 1.67E-5 0.07

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.604 0 YPL266W DIM1 het Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
0.355 2.24E-100 YBR192W RIM2 het Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
0.174 3.57E-24 YBL105C PKC1 het Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
0.173 5.41E-24 YDL217C TIM22 het Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported
0.170 3.82E-23 YPR133C SPN1 het Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
0.142 1.42E-16 YKR079C TRZ1 het tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2
0.127 1.41E-13 YOR192C THI72 hom Transporter of thiamine or related compound; shares sequence similarity with Thi7p
0.123 1.06E-12 YOL144W NOP8 het Nucleolar protein required for 60S ribosomal subunit biogenesis
0.121 1.69E-12 YMR308C PSE1 het Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p
0.121 2.31E-12 YKL195W MIA40 het Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
0.119 4.29E-12 YJL039C NUP192 het Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205
0.116 1.46E-11 YLR355C ILV5 het Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
0.112 8.47E-11 YJR049C UTR1 hom ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
0.111 1.02E-10 YGL064C MRH4 hom Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function
0.109 2.48E-10 YNL252C MRPL17 hom Mitochondrial ribosomal protein of the large subunit