YMR117C / SPC24

Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering

Zygosity: Heterozygous strain
fixedexpanded
Profile for YMR117C / SPC24

Click on Significant Values for Screen Details ID:SGTC_231|Compound:b-estradiol|FD-Score:2.59|P-value:8.84E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:2.85|P-value:2.93E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:2.85|P-value:3.00E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:2.94|P-value:1.99E-4 ID:SGTC_443|Compound:clozapine|FD-Score:3.48|P-value:1.41E-5 ID:SGTC_465|Compound:cgp-37157|FD-Score:3.51|P-value:1.24E-5 ID:SGTC_505|Compound:gw-5074|FD-Score:3.10|P-value:9.68E-5 ID:SGTC_507|Compound:nimesulide|FD-Score:3.60|P-value:7.39E-6 ID:SGTC_620|Compound:0109-0052|FD-Score:-4.10|P-value:5.59E-7 ID:SGTC_685|Compound:1278-0033|FD-Score:3.47|P-value:1.51E-5 ID:SGTC_741|Compound:4582-0002|FD-Score:-3.05|P-value:1.54E-4 ID:SGTC_869|Compound:0564-0080|FD-Score:-3.83|P-value:2.86E-6 ID:SGTC_998|Compound:3996-0085|FD-Score:2.63|P-value:7.70E-4 ID:SGTC_1030|Compound:k029-0042|FD-Score:3.99|P-value:8.37E-7 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.61|P-value:9.57E-6 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.46|P-value:1.57E-5 ID:SGTC_1048|Compound:3161-0300|FD-Score:-2.68|P-value:7.50E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:-3.04|P-value:1.61E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:6.10|P-value:1.26E-13 ID:SGTC_1062|Compound:mesoridazine|FD-Score:-2.79|P-value:4.84E-4 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:-3.02|P-value:1.71E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:2.89|P-value:2.50E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:-2.70|P-value:6.95E-4 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.25|P-value:4.66E-5 ID:SGTC_1365|Compound:1672-3266|FD-Score:2.71|P-value:5.50E-4 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:3.41|P-value:2.08E-5 ID:SGTC_1612|Compound:st002233|FD-Score:-3.19|P-value:7.83E-5 ID:SGTC_1621|Compound:st003705|FD-Score:-3.55|P-value:1.32E-5 ID:SGTC_2052|Compound:5265182|FD-Score:2.87|P-value:2.79E-4 ID:SGTC_2060|Compound:5238662|FD-Score:3.04|P-value:1.29E-4 ID:SGTC_2136|Compound:5328288|FD-Score:5.51|P-value:2.01E-11 ID:SGTC_2332|Compound:7982454|FD-Score:2.65|P-value:6.95E-4 ID:SGTC_2338|Compound:7999974|FD-Score:3.40|P-value:2.14E-5 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-2.98|P-value:2.09E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:-2.81|P-value:4.39E-4 ID:SGTC_2547|Compound:epiafzelechin trimethyl ether|FD-Score:3.18|P-value:6.39E-5 ID:SGTC_2583|Compound:asiatic acid|FD-Score:2.68|P-value:6.24E-4 ID:SGTC_2587|Compound:piperic acid|FD-Score:-3.23|P-value:6.63E-5 ID:SGTC_2644|Compound:guaiazulene|FD-Score:2.57|P-value:9.89E-4 ID:SGTC_2691|Compound:st077356|FD-Score:-3.59|P-value:1.05E-5 ID:SGTC_2696|Compound:st077691|FD-Score:2.81|P-value:3.60E-4 ID:SGTC_2835|Compound:9006588|FD-Score:-2.63|P-value:9.50E-4 ID:SGTC_2979|Compound:9012552|FD-Score:-2.91|P-value:2.85E-4 ID:SGTC_2984|Compound:9022109|FD-Score:-2.67|P-value:8.00E-4 ID:SGTC_2989|Compound:9073649|FD-Score:2.70|P-value:5.80E-4 ID:SGTC_3005|Compound:9076496|FD-Score:-2.94|P-value:2.48E-4 ID:SGTC_3009|Compound:9080819|FD-Score:3.09|P-value:9.78E-5 ID:SGTC_3014|Compound:9081533|FD-Score:3.05|P-value:1.23E-4 ID:SGTC_3349|Compound:9149703|FD-Score:-3.03|P-value:1.70E-4 ID:SGTC_231|Compound:b-estradiol|FD-Score:2.59|P-value:8.84E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:2.85|P-value:2.93E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:2.85|P-value:3.00E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:2.94|P-value:1.99E-4 ID:SGTC_443|Compound:clozapine|FD-Score:3.48|P-value:1.41E-5 ID:SGTC_465|Compound:cgp-37157|FD-Score:3.51|P-value:1.24E-5 ID:SGTC_505|Compound:gw-5074|FD-Score:3.10|P-value:9.68E-5 ID:SGTC_507|Compound:nimesulide|FD-Score:3.60|P-value:7.39E-6 ID:SGTC_620|Compound:0109-0052|FD-Score:-4.10|P-value:5.59E-7 ID:SGTC_685|Compound:1278-0033|FD-Score:3.47|P-value:1.51E-5 ID:SGTC_741|Compound:4582-0002|FD-Score:-3.05|P-value:1.54E-4 ID:SGTC_869|Compound:0564-0080|FD-Score:-3.83|P-value:2.86E-6 ID:SGTC_998|Compound:3996-0085|FD-Score:2.63|P-value:7.70E-4 ID:SGTC_1030|Compound:k029-0042|FD-Score:3.99|P-value:8.37E-7 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.61|P-value:9.57E-6 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.46|P-value:1.57E-5 ID:SGTC_1048|Compound:3161-0300|FD-Score:-2.68|P-value:7.50E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:-3.04|P-value:1.61E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:6.10|P-value:1.26E-13 ID:SGTC_1062|Compound:mesoridazine|FD-Score:-2.79|P-value:4.84E-4 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:-3.02|P-value:1.71E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:2.89|P-value:2.50E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:-2.70|P-value:6.95E-4 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.25|P-value:4.66E-5 ID:SGTC_1365|Compound:1672-3266|FD-Score:2.71|P-value:5.50E-4 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:3.41|P-value:2.08E-5 ID:SGTC_1612|Compound:st002233|FD-Score:-3.19|P-value:7.83E-5 ID:SGTC_1621|Compound:st003705|FD-Score:-3.55|P-value:1.32E-5 ID:SGTC_2052|Compound:5265182|FD-Score:2.87|P-value:2.79E-4 ID:SGTC_2060|Compound:5238662|FD-Score:3.04|P-value:1.29E-4 ID:SGTC_2136|Compound:5328288|FD-Score:5.51|P-value:2.01E-11 ID:SGTC_2332|Compound:7982454|FD-Score:2.65|P-value:6.95E-4 ID:SGTC_2338|Compound:7999974|FD-Score:3.40|P-value:2.14E-5 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-2.98|P-value:2.09E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:-2.81|P-value:4.39E-4 ID:SGTC_2547|Compound:epiafzelechin trimethyl ether|FD-Score:3.18|P-value:6.39E-5 ID:SGTC_2583|Compound:asiatic acid|FD-Score:2.68|P-value:6.24E-4 ID:SGTC_2587|Compound:piperic acid|FD-Score:-3.23|P-value:6.63E-5 ID:SGTC_2644|Compound:guaiazulene|FD-Score:2.57|P-value:9.89E-4 ID:SGTC_2691|Compound:st077356|FD-Score:-3.59|P-value:1.05E-5 ID:SGTC_2696|Compound:st077691|FD-Score:2.81|P-value:3.60E-4 ID:SGTC_2835|Compound:9006588|FD-Score:-2.63|P-value:9.50E-4 ID:SGTC_2979|Compound:9012552|FD-Score:-2.91|P-value:2.85E-4 ID:SGTC_2984|Compound:9022109|FD-Score:-2.67|P-value:8.00E-4 ID:SGTC_2989|Compound:9073649|FD-Score:2.70|P-value:5.80E-4 ID:SGTC_3005|Compound:9076496|FD-Score:-2.94|P-value:2.48E-4 ID:SGTC_3009|Compound:9080819|FD-Score:3.09|P-value:9.78E-5 ID:SGTC_3014|Compound:9081533|FD-Score:3.05|P-value:1.23E-4 ID:SGTC_3349|Compound:9149703|FD-Score:-3.03|P-value:1.70E-4

Top fitness defect scores for YMR117C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1060 3448-7350 6.10 82.0 µM 1.26E-13 Golgi 8 5.15E-10 0.61
2 SGTC_2136 5328288 5.51 199.3 µM 2.01E-11 3 1.83E-8 0.15
3 SGTC_1030 k029-0042 3.99 36.7 µM 8.37E-7 TSC3-RPN4 3 3.35E-5 0.05
4 SGTC_507 nimesulide 3.60 162.0 µM 7.39E-6 2 1.57E-4 0.15
5 SGTC_465 cgp-37157 3.51 41.0 µM 1.24E-5 2 2.27E-4 0.57
6 SGTC_443 clozapine 3.48 122.0 µM 1.41E-5 NEO1-PIK1 6 2.48E-4 0.23
7 SGTC_685 1278-0033 3.47 54.9 µM 1.51E-5 60S ribosome export 11 2.60E-4 0.10
8 SGTC_1047 2043-3044 3.46 82.0 µM 1.57E-5 14 2.67E-4 0.08
9 SGTC_1587 estradiol valerate 3.41 56.1 µM 2.08E-5 6 3.27E-4 0.25
10 SGTC_2338 7999974 3.40 200.0 µM 2.14E-5 14 3.34E-4 0.09
11 SGTC_1333 1482-0047 3.25 286.0 µM 4.66E-5 12 5.80E-4 0.01
12 SGTC_2547 epiafzelechin trimethyl ether 3.18 100.0 µM 6.39E-5 4 7.26E-4 0.13
13 SGTC_505 gw-5074 3.10 63.9 µM 9.68E-5 17 9.77E-4 0.02
14 SGTC_3009 9080819 3.09 71.4 µM 9.78E-5 16 9.84E-4 0.17
15 SGTC_3014 9081533 3.05 49.5 µM 1.23E-4 15 0.00116 0.01
16 SGTC_2060 5238662 3.04 140.0 µM 1.29E-4 14 0.00120 0.01
17 SGTC_425 3825-7017 2.94 13.1 µM 1.99E-4 22 0.00163 0.02
18 SGTC_1100 amiodarone 2.89 5.1 µM 2.50E-4 12 0.00192 0.02
19 SGTC_2052 5265182 2.87 7.1 µM 2.79E-4 22 0.00208 0.01
20 SGTC_245 methotrexate 2.85 289.6 µM 2.93E-4 27 0.00215 0.08

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.110 1.48E-10 YLR406C RPL31B hom Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication
0.108 2.95E-10 YKL049C CSE4 het Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions
0.103 1.87E-9 YGR103W NOP7 het Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G0 and the initiation of cell proliferation
0.098 1.38E-8 YHR152W SPO12 hom Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis
0.095 3.56E-8 YKL078W DHR2 het Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
0.092 9.72E-8 YDR486C VPS60 hom Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p
0.090 1.94E-7 YOR127W RGA1 hom GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.087 4.00E-7 YAL065C_p YAL065C_p hom Putative protein of unknown function; has homology to FLO1; possible pseudogene
0.085 7.65E-7 YOL134C_d YOL134C_d het Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit
0.084 1.16E-6 YGR185C TYS1 het Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress
0.084 1.19E-6 YCL043C PDI1 het Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
0.079 4.89E-6 YNR071C_p YNR071C_p hom Putative aldose 1-epimerase
0.078 5.53E-6 YNR014W_p YNR014W_p hom Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication
0.078 5.77E-6 YNL161W CBK1 het Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
0.078 5.85E-6 YGL238W CSE1 het Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation