YMR123W / PKR1

V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR123W / PKR1

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:5.24|P-value:2.63E-5 ID:SGTC_16|Compound:erodoxin|FD-Score:6.68|P-value:1.19E-7 ID:SGTC_50|Compound:0929-0078|FD-Score:4.34|P-value:4.15E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:4.64|P-value:1.73E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:6.23|P-value:7.34E-7 ID:SGTC_90|Compound:0870-0863|FD-Score:4.99|P-value:5.92E-5 ID:SGTC_112|Compound:phenothiazine|FD-Score:6.30|P-value:5.51E-7 ID:SGTC_113|Compound:0099-0221|FD-Score:-4.16|P-value:5.43E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:4.47|P-value:2.87E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:4.70|P-value:1.44E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:5.25|P-value:2.54E-5 ID:SGTC_153|Compound:4077-0274|FD-Score:4.71|P-value:1.39E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:5.72|P-value:4.99E-6 ID:SGTC_204|Compound:1543-0184|FD-Score:5.22|P-value:2.76E-5 ID:SGTC_263|Compound:alizarin|FD-Score:6.86|P-value:5.72E-8 ID:SGTC_275|Compound:trichlorophene|FD-Score:5.98|P-value:1.88E-6 ID:SGTC_352|Compound:k048-0007|FD-Score:4.58|P-value:2.06E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:5.10|P-value:4.10E-5 ID:SGTC_391|Compound:gliotoxin|FD-Score:6.15|P-value:1.01E-6 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:5.49|P-value:1.11E-5 ID:SGTC_505|Compound:gw-5074|FD-Score:4.16|P-value:6.79E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:5.30|P-value:2.12E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:4.82|P-value:1.00E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:4.18|P-value:6.30E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:4.14|P-value:7.16E-4 ID:SGTC_598|Compound:3937-0236|FD-Score:4.14|P-value:7.08E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:-4.30|P-value:3.68E-4 ID:SGTC_737|Compound:1950-0117|FD-Score:5.35|P-value:1.79E-5 ID:SGTC_745|Compound:0469-0705|FD-Score:5.04|P-value:5.07E-5 ID:SGTC_748|Compound:0136-0228|FD-Score:8.31|P-value:6.33E-11 ID:SGTC_768|Compound:0384-0033|FD-Score:4.47|P-value:2.86E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:9.70|P-value:2.92E-14 ID:SGTC_1119|Compound:parthenolide|FD-Score:-5.09|P-value:3.31E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:-4.72|P-value:1.08E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:4.94|P-value:6.87E-5 ID:SGTC_1155|Compound:4088-0394|FD-Score:4.56|P-value:2.20E-4 ID:SGTC_1201|Compound:1889-3372|FD-Score:4.41|P-value:3.38E-4 ID:SGTC_1323|Compound:1348-1351|FD-Score:-3.94|P-value:9.86E-4 ID:SGTC_1363|Compound:1636-0063|FD-Score:6.12|P-value:1.13E-6 ID:SGTC_1368|Compound:1852-0141|FD-Score:6.00|P-value:1.77E-6 ID:SGTC_1385|Compound:3091-4834|FD-Score:-4.09|P-value:6.58E-4 ID:SGTC_1388|Compound:3346-4487|FD-Score:6.16|P-value:9.78E-7 ID:SGTC_1510|Compound:1123-0200|FD-Score:4.02|P-value:9.79E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:-6.80|P-value:5.32E-8 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:-4.53|P-value:1.90E-4 ID:SGTC_1586|Compound:deoxycorticosterone acetate|FD-Score:-4.15|P-value:5.56E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:4.56|P-value:2.19E-4 ID:SGTC_1629|Compound:st005085|FD-Score:14.90|P-value:4.41E-31 ID:SGTC_1673|Compound:st016611|FD-Score:4.34|P-value:4.15E-4 ID:SGTC_1687|Compound:st020778|FD-Score:7.89|P-value:5.07E-10 ID:SGTC_1718|Compound:roemerine|FD-Score:-7.78|P-value:5.96E-10 ID:SGTC_1729|Compound:st035615|FD-Score:10.00|P-value:4.10E-15 ID:SGTC_1730|Compound:st033808|FD-Score:9.51|P-value:8.73E-14 ID:SGTC_1731|Compound:st035964|FD-Score:5.28|P-value:2.24E-5 ID:SGTC_1873|Compound:rapamycin|FD-Score:7.32|P-value:7.24E-9 ID:SGTC_1881|Compound:5652484|FD-Score:5.70|P-value:5.34E-6 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:6.10|P-value:1.23E-6 ID:SGTC_1884|Compound:5633444|FD-Score:-4.75|P-value:9.69E-5 ID:SGTC_1998|Compound:st075797|FD-Score:4.53|P-value:2.41E-4 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:4.05|P-value:9.06E-4 ID:SGTC_2077|Compound:5212015|FD-Score:5.11|P-value:4.05E-5 ID:SGTC_2102|Compound:5377816|FD-Score:4.10|P-value:7.81E-4 ID:SGTC_2248|Compound:tofa|FD-Score:4.43|P-value:3.22E-4 ID:SGTC_2275|Compound:7947463|FD-Score:5.49|P-value:1.10E-5 ID:SGTC_2316|Compound:5844804|FD-Score:-4.09|P-value:6.68E-4 ID:SGTC_2338|Compound:7999974|FD-Score:-5.25|P-value:1.93E-5 ID:SGTC_2359|Compound:9039622|FD-Score:-4.15|P-value:5.55E-4 ID:SGTC_2416|Compound:tofa|FD-Score:5.60|P-value:7.59E-6 ID:SGTC_2515|Compound:chrysin|FD-Score:4.48|P-value:2.77E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:6.82|P-value:6.64E-8 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:4.23|P-value:5.51E-4 ID:SGTC_2695|Compound:st077686|FD-Score:5.48|P-value:1.15E-5 ID:SGTC_2736|Compound:butenafine|FD-Score:-4.65|P-value:1.31E-4 ID:SGTC_2805|Compound:7950783|FD-Score:-3.98|P-value:8.78E-4 ID:SGTC_2845|Compound:9003024|FD-Score:-4.83|P-value:7.69E-5 ID:SGTC_2925|Compound:9010536|FD-Score:-4.97|P-value:4.93E-5 ID:SGTC_2926|Compound:9010726|FD-Score:-4.99|P-value:4.62E-5 ID:SGTC_2928|Compound:9011484|FD-Score:-4.03|P-value:7.66E-4 ID:SGTC_2930|Compound:9013230|FD-Score:-4.90|P-value:6.07E-5 ID:SGTC_2947|Compound:9072639|FD-Score:-5.24|P-value:1.99E-5 ID:SGTC_3117|Compound:9124945|FD-Score:4.09|P-value:8.09E-4 ID:SGTC_3176|Compound:9106582|FD-Score:-4.07|P-value:7.04E-4 ID:SGTC_10|Compound:0279-0212|FD-Score:5.24|P-value:2.63E-5 ID:SGTC_16|Compound:erodoxin|FD-Score:6.68|P-value:1.19E-7 ID:SGTC_50|Compound:0929-0078|FD-Score:4.34|P-value:4.15E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:4.64|P-value:1.73E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:6.23|P-value:7.34E-7 ID:SGTC_90|Compound:0870-0863|FD-Score:4.99|P-value:5.92E-5 ID:SGTC_112|Compound:phenothiazine|FD-Score:6.30|P-value:5.51E-7 ID:SGTC_113|Compound:0099-0221|FD-Score:-4.16|P-value:5.43E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:4.47|P-value:2.87E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:4.70|P-value:1.44E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:5.25|P-value:2.54E-5 ID:SGTC_153|Compound:4077-0274|FD-Score:4.71|P-value:1.39E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:5.72|P-value:4.99E-6 ID:SGTC_204|Compound:1543-0184|FD-Score:5.22|P-value:2.76E-5 ID:SGTC_263|Compound:alizarin|FD-Score:6.86|P-value:5.72E-8 ID:SGTC_275|Compound:trichlorophene|FD-Score:5.98|P-value:1.88E-6 ID:SGTC_352|Compound:k048-0007|FD-Score:4.58|P-value:2.06E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:5.10|P-value:4.10E-5 ID:SGTC_391|Compound:gliotoxin|FD-Score:6.15|P-value:1.01E-6 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:5.49|P-value:1.11E-5 ID:SGTC_505|Compound:gw-5074|FD-Score:4.16|P-value:6.79E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:5.30|P-value:2.12E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:4.82|P-value:1.00E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:4.18|P-value:6.30E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:4.14|P-value:7.16E-4 ID:SGTC_598|Compound:3937-0236|FD-Score:4.14|P-value:7.08E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:-4.30|P-value:3.68E-4 ID:SGTC_737|Compound:1950-0117|FD-Score:5.35|P-value:1.79E-5 ID:SGTC_745|Compound:0469-0705|FD-Score:5.04|P-value:5.07E-5 ID:SGTC_748|Compound:0136-0228|FD-Score:8.31|P-value:6.33E-11 ID:SGTC_768|Compound:0384-0033|FD-Score:4.47|P-value:2.86E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:9.70|P-value:2.92E-14 ID:SGTC_1119|Compound:parthenolide|FD-Score:-5.09|P-value:3.31E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:-4.72|P-value:1.08E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:4.94|P-value:6.87E-5 ID:SGTC_1155|Compound:4088-0394|FD-Score:4.56|P-value:2.20E-4 ID:SGTC_1201|Compound:1889-3372|FD-Score:4.41|P-value:3.38E-4 ID:SGTC_1323|Compound:1348-1351|FD-Score:-3.94|P-value:9.86E-4 ID:SGTC_1363|Compound:1636-0063|FD-Score:6.12|P-value:1.13E-6 ID:SGTC_1368|Compound:1852-0141|FD-Score:6.00|P-value:1.77E-6 ID:SGTC_1385|Compound:3091-4834|FD-Score:-4.09|P-value:6.58E-4 ID:SGTC_1388|Compound:3346-4487|FD-Score:6.16|P-value:9.78E-7 ID:SGTC_1510|Compound:1123-0200|FD-Score:4.02|P-value:9.79E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:-6.80|P-value:5.32E-8 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:-4.53|P-value:1.90E-4 ID:SGTC_1586|Compound:deoxycorticosterone acetate|FD-Score:-4.15|P-value:5.56E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:4.56|P-value:2.19E-4 ID:SGTC_1629|Compound:st005085|FD-Score:14.90|P-value:4.41E-31 ID:SGTC_1673|Compound:st016611|FD-Score:4.34|P-value:4.15E-4 ID:SGTC_1687|Compound:st020778|FD-Score:7.89|P-value:5.07E-10 ID:SGTC_1718|Compound:roemerine|FD-Score:-7.78|P-value:5.96E-10 ID:SGTC_1729|Compound:st035615|FD-Score:10.00|P-value:4.10E-15 ID:SGTC_1730|Compound:st033808|FD-Score:9.51|P-value:8.73E-14 ID:SGTC_1731|Compound:st035964|FD-Score:5.28|P-value:2.24E-5 ID:SGTC_1873|Compound:rapamycin|FD-Score:7.32|P-value:7.24E-9 ID:SGTC_1881|Compound:5652484|FD-Score:5.70|P-value:5.34E-6 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:6.10|P-value:1.23E-6 ID:SGTC_1884|Compound:5633444|FD-Score:-4.75|P-value:9.69E-5 ID:SGTC_1998|Compound:st075797|FD-Score:4.53|P-value:2.41E-4 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:4.05|P-value:9.06E-4 ID:SGTC_2077|Compound:5212015|FD-Score:5.11|P-value:4.05E-5 ID:SGTC_2102|Compound:5377816|FD-Score:4.10|P-value:7.81E-4 ID:SGTC_2248|Compound:tofa|FD-Score:4.43|P-value:3.22E-4 ID:SGTC_2275|Compound:7947463|FD-Score:5.49|P-value:1.10E-5 ID:SGTC_2316|Compound:5844804|FD-Score:-4.09|P-value:6.68E-4 ID:SGTC_2338|Compound:7999974|FD-Score:-5.25|P-value:1.93E-5 ID:SGTC_2359|Compound:9039622|FD-Score:-4.15|P-value:5.55E-4 ID:SGTC_2416|Compound:tofa|FD-Score:5.60|P-value:7.59E-6 ID:SGTC_2515|Compound:chrysin|FD-Score:4.48|P-value:2.77E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:6.82|P-value:6.64E-8 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:4.23|P-value:5.51E-4 ID:SGTC_2695|Compound:st077686|FD-Score:5.48|P-value:1.15E-5 ID:SGTC_2736|Compound:butenafine|FD-Score:-4.65|P-value:1.31E-4 ID:SGTC_2805|Compound:7950783|FD-Score:-3.98|P-value:8.78E-4 ID:SGTC_2845|Compound:9003024|FD-Score:-4.83|P-value:7.69E-5 ID:SGTC_2925|Compound:9010536|FD-Score:-4.97|P-value:4.93E-5 ID:SGTC_2926|Compound:9010726|FD-Score:-4.99|P-value:4.62E-5 ID:SGTC_2928|Compound:9011484|FD-Score:-4.03|P-value:7.66E-4 ID:SGTC_2930|Compound:9013230|FD-Score:-4.90|P-value:6.07E-5 ID:SGTC_2947|Compound:9072639|FD-Score:-5.24|P-value:1.99E-5 ID:SGTC_3117|Compound:9124945|FD-Score:4.09|P-value:8.09E-4 ID:SGTC_3176|Compound:9106582|FD-Score:-4.07|P-value:7.04E-4

Top fitness defect scores for YMR123W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1629 st005085 14.90 44.9 µM 4.41E-31 RPP1 & pyrimidine depletion 1 2.68E-50
2 SGTC_1729 st035615 10.00 46.0 µM 4.10E-15 2 6.19E-24
3 SGTC_841 0251-0215 9.70 91.2 µM 2.92E-14 15 1.58E-22
4 SGTC_1730 st033808 9.51 45.1 µM 8.73E-14 1 9.59E-22
5 SGTC_748 0136-0228 8.31 77.9 µM 6.33E-11 redox potentiating 14 4.93E-17
6 SGTC_1687 st020778 7.89 55.4 µM 5.07E-10 71 1.51E-15
7 SGTC_1873 rapamycin 7.32 2.0 nM 7.24E-9 17 1.20E-13
8 SGTC_263 alizarin 6.86 107.9 µM 5.72E-8 iron homeostasis 15 3.56E-12
9 SGTC_2567 aureobasidin a 6.82 60.0 nM 6.64E-8 31 4.56E-12
10 SGTC_16 erodoxin 6.68 22.8 µM 1.19E-7 unfolded protein response 22 1.19E-11
11 SGTC_112 phenothiazine 6.30 287.2 µM 5.51E-7 unfolded protein response 3 1.46E-10
12 SGTC_86 0417-1630 6.23 471.9 µM 7.34E-7 ubiquinone biosynthesis & proteasome 85 2.34E-10
13 SGTC_1388 3346-4487 6.16 254.0 µM 9.78E-7 2 3.75E-10
14 SGTC_391 gliotoxin 6.15 8.3 µM 1.01E-6 20 3.94E-10
15 SGTC_1363 1636-0063 6.12 10.4 µM 1.13E-6 Golgi 5 4.76E-10
16 SGTC_1883 5-benzylidenerhodanine 6.10 20.0 µM 1.23E-6 TRP & mitochondrial translation 17 5.47E-10
17 SGTC_1368 1852-0141 6.00 284.0 µM 1.77E-6 ERG2 12 9.90E-10
18 SGTC_275 trichlorophene 5.98 5.7 µM 1.88E-6 mitochondrial stress 121 1.10E-9
19 SGTC_176 1486-1328 5.72 72.8 µM 4.99E-6 24 5.40E-9
20 SGTC_1881 5652484 5.70 16.0 µM 5.34E-6 TRP & mitochondrial translation 12 6.02E-9

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.309 1.51E-74 YJR033C RAV1 hom Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
0.243 7.77E-46 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.222 1.97E-38 YDR203W_d YDR203W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.220 8.50E-38 YKL077W_p YKL077W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.201 1.34E-31 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.194 1.29E-29 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.189 3.79E-28 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.184 8.15E-27 YDR320C SWA2 hom Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles
0.184 1.14E-26 YDL077C VAM6 hom Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
0.174 3.79E-24 YGR167W CLC1 hom Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W
0.174 5.86E-24 YNL323W LEM3 hom Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane
0.171 3.36E-23 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.166 4.10E-22 YDR202C RAV2 hom Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme
0.165 1.14E-21 YLR372W SUR4 hom Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
0.159 2.15E-20 YGR037C ACB1 hom Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress