YMR128W / ECM16

Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis

Zygosity: Heterozygous strain
fixedexpanded
Profile for YMR128W / ECM16

Click on Significant Values for Screen Details ID:SGTC_45|Compound:0kpi-0036|FD-Score:3.08|P-value:6.12E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:3.17|P-value:4.37E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:7.90|P-value:5.66E-17 ID:SGTC_424|Compound:splitomicin|FD-Score:3.83|P-value:3.00E-5 ID:SGTC_426|Compound:0988-0037|FD-Score:3.18|P-value:4.26E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:-3.19|P-value:4.09E-4 ID:SGTC_470|Compound:aa-861|FD-Score:-3.50|P-value:1.19E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:4.58|P-value:7.79E-7 ID:SGTC_718|Compound:1130-0073|FD-Score:3.01|P-value:7.90E-4 ID:SGTC_801|Compound:0866-0028|FD-Score:3.24|P-value:3.36E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:-2.97|P-value:9.15E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:-3.60|P-value:7.90E-5 ID:SGTC_973|Compound:1325-0077|FD-Score:-3.11|P-value:5.44E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:-3.01|P-value:7.98E-4 ID:SGTC_977|Compound:1348-1378|FD-Score:-2.99|P-value:8.49E-4 ID:SGTC_983|Compound:1491-0821|FD-Score:3.90|P-value:2.12E-5 ID:SGTC_984|Compound:1492-0255|FD-Score:3.03|P-value:7.40E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:-3.43|P-value:1.57E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:4.32|P-value:2.97E-6 ID:SGTC_1248|Compound:0469-0687|FD-Score:-2.97|P-value:9.17E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:4.20|P-value:5.37E-6 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.03|P-value:7.36E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.26|P-value:3.17E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:4.75|P-value:3.09E-7 ID:SGTC_1605|Compound:st000082|FD-Score:2.99|P-value:8.50E-4 ID:SGTC_1606|Compound:st000094|FD-Score:4.70|P-value:4.10E-7 ID:SGTC_1613|Compound:st002381|FD-Score:-4.55|P-value:9.01E-7 ID:SGTC_1620|Compound:st003704|FD-Score:-3.09|P-value:6.05E-4 ID:SGTC_1708|Compound:st033234|FD-Score:3.69|P-value:5.44E-5 ID:SGTC_1893|Compound:st059353|FD-Score:3.16|P-value:4.52E-4 ID:SGTC_1939|Compound:st074455|FD-Score:3.40|P-value:1.81E-4 ID:SGTC_1947|Compound:st076332|FD-Score:3.83|P-value:2.89E-5 ID:SGTC_1950|Compound:st077106|FD-Score:3.79|P-value:3.47E-5 ID:SGTC_1955|Compound:wogonin|FD-Score:3.09|P-value:6.02E-4 ID:SGTC_1964|Compound:st076914|FD-Score:3.33|P-value:2.43E-4 ID:SGTC_1966|Compound:st070145|FD-Score:4.16|P-value:6.55E-6 ID:SGTC_2119|Compound:5528445|FD-Score:3.77|P-value:3.92E-5 ID:SGTC_2199|Compound:6590217|FD-Score:-3.26|P-value:3.19E-4 ID:SGTC_2258|Compound:7960471|FD-Score:3.27|P-value:3.07E-4 ID:SGTC_2482|Compound:5981709|FD-Score:3.55|P-value:9.69E-5 ID:SGTC_2511|Compound:berberine|FD-Score:2.98|P-value:8.87E-4 ID:SGTC_2560|Compound:caryophyllene|FD-Score:4.14|P-value:6.96E-6 ID:SGTC_2650|Compound:prasterone|FD-Score:-3.17|P-value:4.45E-4 ID:SGTC_2804|Compound:7954240|FD-Score:3.76|P-value:3.94E-5 ID:SGTC_2810|Compound:7959871|FD-Score:3.07|P-value:6.42E-4 ID:SGTC_2862|Compound:9027634|FD-Score:3.46|P-value:1.40E-4 ID:SGTC_2864|Compound:9034755|FD-Score:4.70|P-value:4.18E-7 ID:SGTC_2873|Compound:9040596|FD-Score:-4.00|P-value:1.38E-5 ID:SGTC_2883|Compound:9059145|FD-Score:3.55|P-value:9.75E-5 ID:SGTC_2889|Compound:9059656|FD-Score:3.02|P-value:7.57E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-8.88|P-value:5.85E-21 ID:SGTC_2952|Compound:9073731|FD-Score:4.21|P-value:4.97E-6 ID:SGTC_2972|Compound:9093619|FD-Score:3.16|P-value:4.55E-4 ID:SGTC_2988|Compound:9073640|FD-Score:4.31|P-value:3.08E-6 ID:SGTC_2989|Compound:9073649|FD-Score:-3.77|P-value:3.88E-5 ID:SGTC_3137|Compound:9097352|FD-Score:4.16|P-value:6.56E-6 ID:SGTC_3155|Compound:9099265|FD-Score:-3.80|P-value:3.31E-5 ID:SGTC_3159|Compound:9100096|FD-Score:-4.47|P-value:1.38E-6 ID:SGTC_3181|Compound:9108277|FD-Score:-3.61|P-value:7.75E-5 ID:SGTC_3193|Compound:9111931|FD-Score:-3.51|P-value:1.17E-4 ID:SGTC_3200|Compound:9114385|FD-Score:4.10|P-value:8.55E-6 ID:SGTC_45|Compound:0kpi-0036|FD-Score:3.08|P-value:6.12E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:3.17|P-value:4.37E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:7.90|P-value:5.66E-17 ID:SGTC_424|Compound:splitomicin|FD-Score:3.83|P-value:3.00E-5 ID:SGTC_426|Compound:0988-0037|FD-Score:3.18|P-value:4.26E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:-3.19|P-value:4.09E-4 ID:SGTC_470|Compound:aa-861|FD-Score:-3.50|P-value:1.19E-4 ID:SGTC_574|Compound:1315-0397|FD-Score:4.58|P-value:7.79E-7 ID:SGTC_718|Compound:1130-0073|FD-Score:3.01|P-value:7.90E-4 ID:SGTC_801|Compound:0866-0028|FD-Score:3.24|P-value:3.36E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:-2.97|P-value:9.15E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:-3.60|P-value:7.90E-5 ID:SGTC_973|Compound:1325-0077|FD-Score:-3.11|P-value:5.44E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:-3.01|P-value:7.98E-4 ID:SGTC_977|Compound:1348-1378|FD-Score:-2.99|P-value:8.49E-4 ID:SGTC_983|Compound:1491-0821|FD-Score:3.90|P-value:2.12E-5 ID:SGTC_984|Compound:1492-0255|FD-Score:3.03|P-value:7.40E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:-3.43|P-value:1.57E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:4.32|P-value:2.97E-6 ID:SGTC_1248|Compound:0469-0687|FD-Score:-2.97|P-value:9.17E-4 ID:SGTC_1258|Compound:0671-0043|FD-Score:4.20|P-value:5.37E-6 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.03|P-value:7.36E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.26|P-value:3.17E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:4.75|P-value:3.09E-7 ID:SGTC_1605|Compound:st000082|FD-Score:2.99|P-value:8.50E-4 ID:SGTC_1606|Compound:st000094|FD-Score:4.70|P-value:4.10E-7 ID:SGTC_1613|Compound:st002381|FD-Score:-4.55|P-value:9.01E-7 ID:SGTC_1620|Compound:st003704|FD-Score:-3.09|P-value:6.05E-4 ID:SGTC_1708|Compound:st033234|FD-Score:3.69|P-value:5.44E-5 ID:SGTC_1893|Compound:st059353|FD-Score:3.16|P-value:4.52E-4 ID:SGTC_1939|Compound:st074455|FD-Score:3.40|P-value:1.81E-4 ID:SGTC_1947|Compound:st076332|FD-Score:3.83|P-value:2.89E-5 ID:SGTC_1950|Compound:st077106|FD-Score:3.79|P-value:3.47E-5 ID:SGTC_1955|Compound:wogonin|FD-Score:3.09|P-value:6.02E-4 ID:SGTC_1964|Compound:st076914|FD-Score:3.33|P-value:2.43E-4 ID:SGTC_1966|Compound:st070145|FD-Score:4.16|P-value:6.55E-6 ID:SGTC_2119|Compound:5528445|FD-Score:3.77|P-value:3.92E-5 ID:SGTC_2199|Compound:6590217|FD-Score:-3.26|P-value:3.19E-4 ID:SGTC_2258|Compound:7960471|FD-Score:3.27|P-value:3.07E-4 ID:SGTC_2482|Compound:5981709|FD-Score:3.55|P-value:9.69E-5 ID:SGTC_2511|Compound:berberine|FD-Score:2.98|P-value:8.87E-4 ID:SGTC_2560|Compound:caryophyllene|FD-Score:4.14|P-value:6.96E-6 ID:SGTC_2650|Compound:prasterone|FD-Score:-3.17|P-value:4.45E-4 ID:SGTC_2804|Compound:7954240|FD-Score:3.76|P-value:3.94E-5 ID:SGTC_2810|Compound:7959871|FD-Score:3.07|P-value:6.42E-4 ID:SGTC_2862|Compound:9027634|FD-Score:3.46|P-value:1.40E-4 ID:SGTC_2864|Compound:9034755|FD-Score:4.70|P-value:4.18E-7 ID:SGTC_2873|Compound:9040596|FD-Score:-4.00|P-value:1.38E-5 ID:SGTC_2883|Compound:9059145|FD-Score:3.55|P-value:9.75E-5 ID:SGTC_2889|Compound:9059656|FD-Score:3.02|P-value:7.57E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-8.88|P-value:5.85E-21 ID:SGTC_2952|Compound:9073731|FD-Score:4.21|P-value:4.97E-6 ID:SGTC_2972|Compound:9093619|FD-Score:3.16|P-value:4.55E-4 ID:SGTC_2988|Compound:9073640|FD-Score:4.31|P-value:3.08E-6 ID:SGTC_2989|Compound:9073649|FD-Score:-3.77|P-value:3.88E-5 ID:SGTC_3137|Compound:9097352|FD-Score:4.16|P-value:6.56E-6 ID:SGTC_3155|Compound:9099265|FD-Score:-3.80|P-value:3.31E-5 ID:SGTC_3159|Compound:9100096|FD-Score:-4.47|P-value:1.38E-6 ID:SGTC_3181|Compound:9108277|FD-Score:-3.61|P-value:7.75E-5 ID:SGTC_3193|Compound:9111931|FD-Score:-3.51|P-value:1.17E-4 ID:SGTC_3200|Compound:9114385|FD-Score:4.10|P-value:8.55E-6

Top fitness defect scores for YMR128W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_366 0181-0206 7.90 4.3 µM 5.66E-17 Golgi 2 1.35E-15 1.73
2 SGTC_1600 coumarin 106 4.75 71.1 µM 3.09E-7 3 1.00E-6 1.02
3 SGTC_1606 st000094 4.70 49.2 µM 4.10E-7 60S ribosome export 2 1.29E-6 0.36
4 SGTC_2864 9034755 4.70 47.6 µM 4.18E-7 3 1.32E-6 0.56
5 SGTC_574 1315-0397 4.58 40.0 µM 7.79E-7 6 2.32E-6 0.13
6 SGTC_1134 3477-0009 4.32 42.5 µM 2.97E-6 7 7.89E-6 0.02
7 SGTC_2988 9073640 4.31 71.4 µM 3.08E-6 10 8.15E-6 0.16
8 SGTC_2952 9073731 4.21 13.8 µM 4.97E-6 tubulin folding & SWR complex 11 1.26E-5 0.09
9 SGTC_1258 0671-0043 4.20 57.1 µM 5.37E-6 4 1.35E-5 0.09
10 SGTC_1966 st070145 4.16 16.1 µM 6.55E-6 5 1.62E-5 0.20
11 SGTC_3137 9097352 4.16 49.5 µM 6.56E-6 5 1.62E-5 0.25
12 SGTC_2560 caryophyllene 4.14 100.0 µM 6.96E-6 7 1.71E-5 0.52
13 SGTC_3200 9114385 4.10 49.5 µM 8.55E-6 18 2.07E-5 0.01
14 SGTC_983 1491-0821 3.90 12.2 µM 2.12E-5 5 4.74E-5 0.18
15 SGTC_1947 st076332 3.83 61.6 µM 2.89E-5 3 6.29E-5 0.07
16 SGTC_424 splitomicin 3.83 100.0 mM 3.00E-5 4 6.51E-5 0.00
17 SGTC_1950 st077106 3.79 7.2 µM 3.47E-5 RNA processing & uracil transport 8 7.44E-5 0.19
18 SGTC_2119 5528445 3.77 120.6 µM 3.92E-5 4 8.32E-5 0.28
19 SGTC_2804 7954240 3.76 19.5 µM 3.94E-5 13 8.34E-5 0.11
20 SGTC_1708 st033234 3.69 31.8 µM 5.44E-5 5 1.12E-4 0.22

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.118 5.85E-12 YOR181W LAS17 het Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)
0.110 1.82E-10 YHR068W DYS1 het Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric
0.107 5.28E-10 YOR169C_d YOR169C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W
0.102 3.10E-9 YDL241W_p YDL241W_p hom Putative protein of unknown function; YDL241W is not an essential gene
0.086 5.54E-7 YHL043W ECM34 hom Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
0.086 5.71E-7 YLL060C GTT2 hom Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress
0.084 1.02E-6 YBR195C MSI1 hom Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase
0.083 1.66E-6 YDR190C RVB1 het ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
0.081 3.11E-6 YFL040W_p YFL040W_p hom Putative transporter, member of the sugar porter family; YFL040W is not an essential gene
0.079 4.33E-6 YBR145W ADH5 hom Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication
0.078 6.29E-6 YPR175W DPB2 het Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate
0.077 7.60E-6 YMR242W-A_p YMR242W-A_p hom Putative protein of unknown function
0.077 8.31E-6 YNR017W TIM23 het Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel
0.077 8.75E-6 YGR071C ENV11 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
0.076 9.63E-6 YNL088W TOP2 het Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation