YMR135C / GID8

Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR135C / GID8

Click on Significant Values for Screen Details ID:SGTC_52|Compound:0329-0193|FD-Score:3.33|P-value:6.11E-4 ID:SGTC_53|Compound:0973-0023|FD-Score:4.00|P-value:4.99E-5 ID:SGTC_87|Compound:0100-0052|FD-Score:3.81|P-value:1.08E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:-3.20|P-value:9.92E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:4.90|P-value:9.95E-7 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.70|P-value:1.76E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.34|P-value:5.77E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.09|P-value:3.86E-5 ID:SGTC_373|Compound:0844-0104|FD-Score:5.22|P-value:1.96E-7 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:3.54|P-value:2.90E-4 ID:SGTC_405|Compound:ebelactone b|FD-Score:4.37|P-value:1.07E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:6.61|P-value:6.86E-11 ID:SGTC_599|Compound:1935-0052|FD-Score:-4.43|P-value:9.29E-6 ID:SGTC_735|Compound:3076-0345|FD-Score:-3.62|P-value:2.29E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:3.63|P-value:2.10E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:-3.27|P-value:7.87E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.54|P-value:2.94E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:3.48|P-value:3.64E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:3.60|P-value:2.36E-4 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:3.34|P-value:5.85E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:-4.08|P-value:4.00E-5 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.83|P-value:9.88E-5 ID:SGTC_1596|Compound:piperlongumine|FD-Score:-3.86|P-value:9.39E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-5.92|P-value:5.11E-9 ID:SGTC_1745|Compound:st081495|FD-Score:-4.48|P-value:7.22E-6 ID:SGTC_1750|Compound:st081859|FD-Score:4.13|P-value:2.99E-5 ID:SGTC_1898|Compound:st060199|FD-Score:-3.46|P-value:4.16E-4 ID:SGTC_1944|Compound:st076371|FD-Score:-3.21|P-value:9.72E-4 ID:SGTC_2062|Compound:5245942|FD-Score:-3.44|P-value:4.41E-4 ID:SGTC_2081|Compound:5214970|FD-Score:3.45|P-value:4.05E-4 ID:SGTC_2172|Compound:5754775|FD-Score:3.78|P-value:1.21E-4 ID:SGTC_2223|Compound:6903751|FD-Score:4.30|P-value:1.47E-5 ID:SGTC_2296|Compound:7971656|FD-Score:3.54|P-value:2.90E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:-3.24|P-value:8.73E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.02|P-value:4.77E-5 ID:SGTC_2696|Compound:st077691|FD-Score:3.26|P-value:7.65E-4 ID:SGTC_2845|Compound:9003024|FD-Score:3.24|P-value:8.07E-4 ID:SGTC_2913|Compound:7966556|FD-Score:3.23|P-value:8.57E-4 ID:SGTC_2947|Compound:9072639|FD-Score:-3.67|P-value:1.96E-4 ID:SGTC_2978|Compound:9011784|FD-Score:-4.59|P-value:4.47E-6 ID:SGTC_2981|Compound:9013952|FD-Score:-4.58|P-value:4.65E-6 ID:SGTC_2982|Compound:9019404|FD-Score:4.17|P-value:2.60E-5 ID:SGTC_3020|Compound:9089140|FD-Score:-3.69|P-value:1.79E-4 ID:SGTC_3044|Compound:9093193|FD-Score:5.19|P-value:2.34E-7 ID:SGTC_3049|Compound:9092223|FD-Score:-3.91|P-value:7.84E-5 ID:SGTC_52|Compound:0329-0193|FD-Score:3.33|P-value:6.11E-4 ID:SGTC_53|Compound:0973-0023|FD-Score:4.00|P-value:4.99E-5 ID:SGTC_87|Compound:0100-0052|FD-Score:3.81|P-value:1.08E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:-3.20|P-value:9.92E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:4.90|P-value:9.95E-7 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.70|P-value:1.76E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.34|P-value:5.77E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.09|P-value:3.86E-5 ID:SGTC_373|Compound:0844-0104|FD-Score:5.22|P-value:1.96E-7 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:3.54|P-value:2.90E-4 ID:SGTC_405|Compound:ebelactone b|FD-Score:4.37|P-value:1.07E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:6.61|P-value:6.86E-11 ID:SGTC_599|Compound:1935-0052|FD-Score:-4.43|P-value:9.29E-6 ID:SGTC_735|Compound:3076-0345|FD-Score:-3.62|P-value:2.29E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:3.63|P-value:2.10E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:-3.27|P-value:7.87E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.54|P-value:2.94E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:3.48|P-value:3.64E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:3.60|P-value:2.36E-4 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:3.34|P-value:5.85E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:-4.08|P-value:4.00E-5 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.83|P-value:9.88E-5 ID:SGTC_1596|Compound:piperlongumine|FD-Score:-3.86|P-value:9.39E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-5.92|P-value:5.11E-9 ID:SGTC_1745|Compound:st081495|FD-Score:-4.48|P-value:7.22E-6 ID:SGTC_1750|Compound:st081859|FD-Score:4.13|P-value:2.99E-5 ID:SGTC_1898|Compound:st060199|FD-Score:-3.46|P-value:4.16E-4 ID:SGTC_1944|Compound:st076371|FD-Score:-3.21|P-value:9.72E-4 ID:SGTC_2062|Compound:5245942|FD-Score:-3.44|P-value:4.41E-4 ID:SGTC_2081|Compound:5214970|FD-Score:3.45|P-value:4.05E-4 ID:SGTC_2172|Compound:5754775|FD-Score:3.78|P-value:1.21E-4 ID:SGTC_2223|Compound:6903751|FD-Score:4.30|P-value:1.47E-5 ID:SGTC_2296|Compound:7971656|FD-Score:3.54|P-value:2.90E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:-3.24|P-value:8.73E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.02|P-value:4.77E-5 ID:SGTC_2696|Compound:st077691|FD-Score:3.26|P-value:7.65E-4 ID:SGTC_2845|Compound:9003024|FD-Score:3.24|P-value:8.07E-4 ID:SGTC_2913|Compound:7966556|FD-Score:3.23|P-value:8.57E-4 ID:SGTC_2947|Compound:9072639|FD-Score:-3.67|P-value:1.96E-4 ID:SGTC_2978|Compound:9011784|FD-Score:-4.59|P-value:4.47E-6 ID:SGTC_2981|Compound:9013952|FD-Score:-4.58|P-value:4.65E-6 ID:SGTC_2982|Compound:9019404|FD-Score:4.17|P-value:2.60E-5 ID:SGTC_3020|Compound:9089140|FD-Score:-3.69|P-value:1.79E-4 ID:SGTC_3044|Compound:9093193|FD-Score:5.19|P-value:2.34E-7 ID:SGTC_3049|Compound:9092223|FD-Score:-3.91|P-value:7.84E-5

Top fitness defect scores for YMR135C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_453 enantio-paf c16 6.61 10.0 µM 6.86E-11 33 1.89E-11
2 SGTC_373 0844-0104 5.22 25.9 µM 1.96E-7 ERAD & cell cycle 27 8.76E-8
3 SGTC_3044 9093193 5.19 49.5 µM 2.34E-7 7 1.06E-7
4 SGTC_236 1509-0011 4.90 5.3 µM 9.95E-7 330 4.90E-7
5 SGTC_405 ebelactone b 4.37 50.0 µM 1.07E-5 8 6.07E-6
6 SGTC_2223 6903751 4.30 107.6 µM 1.47E-5 superoxide 9 8.52E-6
7 SGTC_2982 9019404 4.17 26.0 µM 2.60E-5 17 1.56E-5
8 SGTC_1750 st081859 4.13 2.4 µM 2.99E-5 endomembrane recycling 172 1.80E-5
9 SGTC_2679 rapamycin 4.02 1.0 nM 4.77E-5 141 2.96E-5
10 SGTC_53 0973-0023 4.00 40.0 µM 4.99E-5 RNA processing & uracil transport 14 3.10E-5
11 SGTC_1592 4',5'-dihydroxyflavone 3.83 8.8 µM 9.88E-5 60S ribosome export 13 6.39E-5
12 SGTC_87 0100-0052 3.81 35.5 µM 1.08E-4 119 7.01E-5
13 SGTC_2172 5754775 3.78 163.9 µM 1.21E-4 18 7.89E-5
14 SGTC_947 3447-0010 3.63 211.0 µM 2.10E-4 21 1.42E-4
15 SGTC_1393 3470-5649 3.60 58.2 µM 2.36E-4 60S ribosome export 21 1.60E-4
16 SGTC_2296 7971656 3.54 126.5 µM 2.90E-4 57 2.00E-4
17 SGTC_388 3',4'-dichloroisocoumarin 3.54 16.7 µM 2.90E-4 23 2.00E-4
18 SGTC_1282 0886-0010 3.54 32.4 µM 2.94E-4 endomembrane recycling 44 2.03E-4
19 SGTC_1353 1498-1206 3.48 8.1 µM 3.64E-4 mitochondrial response to ROS 77 2.54E-4
20 SGTC_2081 5214970 3.45 32.3 µM 4.05E-4 36 2.84E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.150 2.23E-18 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.144 5.90E-17 YKL087C CYT2 hom Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1
0.141 2.23E-16 YGL107C RMD9 hom Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes
0.139 5.63E-16 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.134 5.29E-15 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.130 3.61E-14 YGL218W_d YGL218W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance
0.127 1.49E-13 YDR255C RMD5 hom Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain
0.126 2.54E-13 YJL046W AIM22 hom Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss
0.126 3.00E-13 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.125 4.34E-13 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.124 4.57E-13 YMR214W SCJ1 hom One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins
0.124 5.23E-13 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.124 6.17E-13 YER171W RAD3 het 5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress
0.124 6.66E-13 YOR073W SGO1 hom Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability
0.123 7.65E-13 YDR417C_d YDR417C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W