YMR140W / SIP5

Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR140W / SIP5

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:-4.05|P-value:3.17E-5 ID:SGTC_47|Compound:0149-0135|FD-Score:-7.84|P-value:3.48E-15 ID:SGTC_48|Compound:0382-0018|FD-Score:-6.72|P-value:1.31E-11 ID:SGTC_59|Compound:0330-0160|FD-Score:-4.31|P-value:1.05E-5 ID:SGTC_62|Compound:0180-0423|FD-Score:-4.49|P-value:4.65E-6 ID:SGTC_63|Compound:0099-0242|FD-Score:3.11|P-value:7.98E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:-3.37|P-value:4.61E-4 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.31|P-value:4.03E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.60|P-value:1.37E-4 ID:SGTC_697|Compound:0508-1955|FD-Score:3.44|P-value:2.52E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:3.57|P-value:1.53E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:3.63|P-value:1.19E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:3.09|P-value:8.61E-4 ID:SGTC_1198|Compound:1612-1005|FD-Score:3.26|P-value:4.84E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.93|P-value:3.64E-5 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.52|P-value:1.86E-4 ID:SGTC_1480|Compound:1070-0044|FD-Score:3.73|P-value:7.95E-5 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.22|P-value:5.58E-4 ID:SGTC_1814|Compound:st052110|FD-Score:-3.52|P-value:2.60E-4 ID:SGTC_1878|Compound:temozolomide|FD-Score:3.68|P-value:9.93E-5 ID:SGTC_1910|Compound:st062352|FD-Score:-3.22|P-value:7.65E-4 ID:SGTC_1911|Compound:st060208|FD-Score:-4.43|P-value:6.05E-6 ID:SGTC_2030|Compound:5175110|FD-Score:-3.22|P-value:7.76E-4 ID:SGTC_2082|Compound:5219693|FD-Score:-3.20|P-value:8.25E-4 ID:SGTC_2154|Compound:5570129|FD-Score:3.86|P-value:4.81E-5 ID:SGTC_2216|Compound:7189485|FD-Score:-3.20|P-value:8.13E-4 ID:SGTC_2379|Compound:9035213|FD-Score:-3.43|P-value:3.70E-4 ID:SGTC_2386|Compound:9075165|FD-Score:-3.30|P-value:5.90E-4 ID:SGTC_2390|Compound:9097792|FD-Score:-3.22|P-value:7.81E-4 ID:SGTC_2438|Compound:5259590|FD-Score:-3.59|P-value:2.05E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:3.20|P-value:5.91E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:4.23|P-value:9.84E-6 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.52|P-value:1.83E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.34|P-value:3.54E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.11|P-value:8.13E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.38|P-value:4.79E-6 ID:SGTC_2712|Compound:st079126|FD-Score:-3.41|P-value:3.92E-4 ID:SGTC_2830|Compound:7992007|FD-Score:-4.01|P-value:3.78E-5 ID:SGTC_2831|Compound:7999036|FD-Score:3.34|P-value:3.60E-4 ID:SGTC_2832|Compound:7992762|FD-Score:-7.33|P-value:1.79E-13 ID:SGTC_2989|Compound:9073649|FD-Score:3.33|P-value:3.66E-4 ID:SGTC_3263|Compound:9137018|FD-Score:3.15|P-value:7.00E-4 ID:SGTC_3339|Compound:9145883|FD-Score:3.13|P-value:7.50E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:-4.05|P-value:3.17E-5 ID:SGTC_47|Compound:0149-0135|FD-Score:-7.84|P-value:3.48E-15 ID:SGTC_48|Compound:0382-0018|FD-Score:-6.72|P-value:1.31E-11 ID:SGTC_59|Compound:0330-0160|FD-Score:-4.31|P-value:1.05E-5 ID:SGTC_62|Compound:0180-0423|FD-Score:-4.49|P-value:4.65E-6 ID:SGTC_63|Compound:0099-0242|FD-Score:3.11|P-value:7.98E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:-3.37|P-value:4.61E-4 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.31|P-value:4.03E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.60|P-value:1.37E-4 ID:SGTC_697|Compound:0508-1955|FD-Score:3.44|P-value:2.52E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:3.57|P-value:1.53E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:3.63|P-value:1.19E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:3.09|P-value:8.61E-4 ID:SGTC_1198|Compound:1612-1005|FD-Score:3.26|P-value:4.84E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.93|P-value:3.64E-5 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.52|P-value:1.86E-4 ID:SGTC_1480|Compound:1070-0044|FD-Score:3.73|P-value:7.95E-5 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.22|P-value:5.58E-4 ID:SGTC_1814|Compound:st052110|FD-Score:-3.52|P-value:2.60E-4 ID:SGTC_1878|Compound:temozolomide|FD-Score:3.68|P-value:9.93E-5 ID:SGTC_1910|Compound:st062352|FD-Score:-3.22|P-value:7.65E-4 ID:SGTC_1911|Compound:st060208|FD-Score:-4.43|P-value:6.05E-6 ID:SGTC_2030|Compound:5175110|FD-Score:-3.22|P-value:7.76E-4 ID:SGTC_2082|Compound:5219693|FD-Score:-3.20|P-value:8.25E-4 ID:SGTC_2154|Compound:5570129|FD-Score:3.86|P-value:4.81E-5 ID:SGTC_2216|Compound:7189485|FD-Score:-3.20|P-value:8.13E-4 ID:SGTC_2379|Compound:9035213|FD-Score:-3.43|P-value:3.70E-4 ID:SGTC_2386|Compound:9075165|FD-Score:-3.30|P-value:5.90E-4 ID:SGTC_2390|Compound:9097792|FD-Score:-3.22|P-value:7.81E-4 ID:SGTC_2438|Compound:5259590|FD-Score:-3.59|P-value:2.05E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:3.20|P-value:5.91E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:4.23|P-value:9.84E-6 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.52|P-value:1.83E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:3.34|P-value:3.54E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.11|P-value:8.13E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.38|P-value:4.79E-6 ID:SGTC_2712|Compound:st079126|FD-Score:-3.41|P-value:3.92E-4 ID:SGTC_2830|Compound:7992007|FD-Score:-4.01|P-value:3.78E-5 ID:SGTC_2831|Compound:7999036|FD-Score:3.34|P-value:3.60E-4 ID:SGTC_2832|Compound:7992762|FD-Score:-7.33|P-value:1.79E-13 ID:SGTC_2989|Compound:9073649|FD-Score:3.33|P-value:3.66E-4 ID:SGTC_3263|Compound:9137018|FD-Score:3.15|P-value:7.00E-4 ID:SGTC_3339|Compound:9145883|FD-Score:3.13|P-value:7.50E-4

Top fitness defect scores for YMR140W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2679 rapamycin 4.38 1.0 nM 4.79E-6 123 5.80E-6
2 SGTC_2536 dehydroabietamide 4.23 32.0 µM 9.84E-6 28 1.18E-5
3 SGTC_1205 4161-2785 3.93 76.0 µM 3.64E-5 32 4.33E-5
4 SGTC_2154 5570129 3.86 200.0 µM 4.81E-5 18 5.72E-5
5 SGTC_1480 1070-0044 3.73 41.2 µM 7.95E-5 26 9.42E-5
6 SGTC_1878 temozolomide 3.68 1.0 mM 9.93E-5 12 1.17E-4
7 SGTC_972 1319-0219 3.63 195.0 µM 1.19E-4 41 1.40E-4
8 SGTC_358 0075-0058 3.60 9.4 µM 1.37E-4 78 1.62E-4
9 SGTC_833 3852-0053 3.57 558.0 µM 1.53E-4 24 1.80E-4
10 SGTC_2566 crystal violet carbinol 3.52 226.0 nM 1.83E-4 54 2.15E-4
11 SGTC_1473 k844-0725 3.52 134.0 µM 1.86E-4 27 2.19E-4
12 SGTC_697 0508-1955 3.44 564.0 µM 2.52E-4 55 2.95E-4
13 SGTC_2580 diallyl disulphide 3.34 81.4 µM 3.54E-4 37 4.14E-4
14 SGTC_2831 7999036 3.34 19.5 µM 3.60E-4 74 4.21E-4
15 SGTC_2989 9073649 3.33 71.4 µM 3.66E-4 69 4.28E-4
16 SGTC_261 5-fluorouridine 3.31 15.4 µM 4.03E-4 exosome 37 4.70E-4
17 SGTC_1198 1612-1005 3.26 86.7 µM 4.84E-4 34 5.64E-4
18 SGTC_1525 8-methoxypsoralen 3.22 92.5 µM 5.58E-4 DNA damage response 65 6.49E-4
19 SGTC_2504 purpurogallin 3.20 52.3 µM 5.91E-4 RPP1 & pyrimidine depletion 46 6.87E-4
20 SGTC_3263 9137018 3.15 49.5 µM 7.00E-4 50 8.12E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.132 1.65E-14 YNL104C LEU4 hom Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication
0.117 1.04E-11 YBL002W HTB2 hom Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
0.115 2.85E-11 YLR224W_p YLR224W_p hom F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene
0.111 1.09E-10 YML065W ORC1 het Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity
0.101 4.01E-9 YGR238C KEL2 hom Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate
0.100 6.30E-9 YDR420W HKR1 hom Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection
0.097 2.00E-8 YJR012C_p YJR012C_p het Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W
0.096 2.40E-8 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.092 1.08E-7 YML023C NSE5 het Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
0.091 1.19E-7 YHR007C-A_p YHR007C-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.090 1.86E-7 YIL152W_p YIL152W_p hom Putative protein of unknown function
0.089 2.31E-7 YNL049C SFB2 hom Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p
0.086 6.38E-7 YJR072C NPA3 het Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress
0.085 7.57E-7 YMR182W-A_p YMR182W-A_p hom Putative protein of unknown function
0.084 9.84E-7 YGR161C_p RTS3_p hom Putative component of the protein phosphatase type 2A complex