YMR146C / TIF34

eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YMR146C / TIF34

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:-3.92|P-value:7.00E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:8.42|P-value:6.97E-14 ID:SGTC_132|Compound:0417-1715|FD-Score:4.22|P-value:7.50E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:3.44|P-value:8.95E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:3.70|P-value:4.08E-4 ID:SGTC_257|Compound:daunorubicin|FD-Score:9.98|P-value:1.20E-18 ID:SGTC_302|Compound:1094-0063|FD-Score:5.64|P-value:2.73E-7 ID:SGTC_309|Compound:k408-0051|FD-Score:3.62|P-value:5.20E-4 ID:SGTC_330|Compound:1488-0319|FD-Score:3.78|P-value:3.20E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.93|P-value:1.97E-4 ID:SGTC_605|Compound:1315-0399|FD-Score:5.20|P-value:1.82E-6 ID:SGTC_752|Compound:1896-0090|FD-Score:3.92|P-value:2.06E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:11.40|P-value:1.35E-23 ID:SGTC_800|Compound:3448-7362|FD-Score:3.99|P-value:1.64E-4 ID:SGTC_859|Compound:0439-0102|FD-Score:4.08|P-value:1.22E-4 ID:SGTC_864|Compound:0469-0573|FD-Score:4.12|P-value:1.06E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:8.93|P-value:2.29E-15 ID:SGTC_894|Compound:0960-0238|FD-Score:6.75|P-value:1.20E-9 ID:SGTC_1036|Compound:k216-0215|FD-Score:-4.18|P-value:3.12E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:-4.64|P-value:6.84E-5 ID:SGTC_1101|Compound:ebselen|FD-Score:4.00|P-value:1.55E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:5.04|P-value:3.43E-6 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.94|P-value:1.90E-4 ID:SGTC_1250|Compound:0485-0349|FD-Score:4.15|P-value:9.47E-5 ID:SGTC_1397|Compound:3532-0190|FD-Score:4.52|P-value:2.58E-5 ID:SGTC_1456|Compound:k015-0012|FD-Score:4.94|P-value:5.18E-6 ID:SGTC_1514|Compound:1189-1591|FD-Score:10.70|P-value:4.33E-21 ID:SGTC_1518|Compound:st038567|FD-Score:-5.34|P-value:5.02E-6 ID:SGTC_1522|Compound:st012842|FD-Score:-4.78|P-value:4.08E-5 ID:SGTC_1529|Compound:apigenin|FD-Score:-6.15|P-value:1.59E-7 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:4.61|P-value:1.87E-5 ID:SGTC_1547|Compound:harmine|FD-Score:-4.00|P-value:5.42E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:-3.86|P-value:8.33E-4 ID:SGTC_1644|Compound:st010134|FD-Score:3.67|P-value:4.50E-4 ID:SGTC_1647|Compound:st011722|FD-Score:4.08|P-value:1.19E-4 ID:SGTC_1716|Compound:st033144|FD-Score:-4.09|P-value:4.15E-4 ID:SGTC_1720|Compound:st037298|FD-Score:4.85|P-value:7.41E-6 ID:SGTC_1731|Compound:st035964|FD-Score:-6.28|P-value:8.56E-8 ID:SGTC_1733|Compound:st037357|FD-Score:-5.12|P-value:1.18E-5 ID:SGTC_1734|Compound:st037168|FD-Score:-5.58|P-value:1.89E-6 ID:SGTC_1775|Compound:st081588|FD-Score:-3.80|P-value:9.85E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-6.16|P-value:1.52E-7 ID:SGTC_2015|Compound:5119600|FD-Score:3.65|P-value:4.81E-4 ID:SGTC_2166|Compound:5933061|FD-Score:3.83|P-value:2.71E-4 ID:SGTC_2205|Compound:7238770|FD-Score:3.44|P-value:9.09E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:3.65|P-value:4.73E-4 ID:SGTC_2346|Compound:9031566|FD-Score:6.83|P-value:7.94E-10 ID:SGTC_2399|Compound:5884133|FD-Score:4.78|P-value:9.81E-6 ID:SGTC_2414|Compound:st077232|FD-Score:4.20|P-value:8.02E-5 ID:SGTC_2439|Compound:5492030|FD-Score:3.47|P-value:8.10E-4 ID:SGTC_2445|Compound:5742597|FD-Score:3.41|P-value:9.67E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.46|P-value:8.41E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:-4.60|P-value:7.67E-5 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.55|P-value:6.55E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:-3.87|P-value:7.97E-4 ID:SGTC_2761|Compound:levamisole|FD-Score:4.63|P-value:1.71E-5 ID:SGTC_2766|Compound:oltipraz|FD-Score:-4.06|P-value:4.56E-4 ID:SGTC_2870|Compound:9038528|FD-Score:3.97|P-value:1.75E-4 ID:SGTC_2875|Compound:9039966|FD-Score:-3.87|P-value:8.06E-4 ID:SGTC_3075|Compound:9116269|FD-Score:5.82|P-value:1.20E-7 ID:SGTC_3286|Compound:9117135|FD-Score:5.17|P-value:2.01E-6 ID:SGTC_36|Compound:0335-0881|FD-Score:-3.92|P-value:7.00E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:8.42|P-value:6.97E-14 ID:SGTC_132|Compound:0417-1715|FD-Score:4.22|P-value:7.50E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:3.44|P-value:8.95E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:3.70|P-value:4.08E-4 ID:SGTC_257|Compound:daunorubicin|FD-Score:9.98|P-value:1.20E-18 ID:SGTC_302|Compound:1094-0063|FD-Score:5.64|P-value:2.73E-7 ID:SGTC_309|Compound:k408-0051|FD-Score:3.62|P-value:5.20E-4 ID:SGTC_330|Compound:1488-0319|FD-Score:3.78|P-value:3.20E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.93|P-value:1.97E-4 ID:SGTC_605|Compound:1315-0399|FD-Score:5.20|P-value:1.82E-6 ID:SGTC_752|Compound:1896-0090|FD-Score:3.92|P-value:2.06E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:11.40|P-value:1.35E-23 ID:SGTC_800|Compound:3448-7362|FD-Score:3.99|P-value:1.64E-4 ID:SGTC_859|Compound:0439-0102|FD-Score:4.08|P-value:1.22E-4 ID:SGTC_864|Compound:0469-0573|FD-Score:4.12|P-value:1.06E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:8.93|P-value:2.29E-15 ID:SGTC_894|Compound:0960-0238|FD-Score:6.75|P-value:1.20E-9 ID:SGTC_1036|Compound:k216-0215|FD-Score:-4.18|P-value:3.12E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:-4.64|P-value:6.84E-5 ID:SGTC_1101|Compound:ebselen|FD-Score:4.00|P-value:1.55E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:5.04|P-value:3.43E-6 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.94|P-value:1.90E-4 ID:SGTC_1250|Compound:0485-0349|FD-Score:4.15|P-value:9.47E-5 ID:SGTC_1397|Compound:3532-0190|FD-Score:4.52|P-value:2.58E-5 ID:SGTC_1456|Compound:k015-0012|FD-Score:4.94|P-value:5.18E-6 ID:SGTC_1514|Compound:1189-1591|FD-Score:10.70|P-value:4.33E-21 ID:SGTC_1518|Compound:st038567|FD-Score:-5.34|P-value:5.02E-6 ID:SGTC_1522|Compound:st012842|FD-Score:-4.78|P-value:4.08E-5 ID:SGTC_1529|Compound:apigenin|FD-Score:-6.15|P-value:1.59E-7 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:4.61|P-value:1.87E-5 ID:SGTC_1547|Compound:harmine|FD-Score:-4.00|P-value:5.42E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:-3.86|P-value:8.33E-4 ID:SGTC_1644|Compound:st010134|FD-Score:3.67|P-value:4.50E-4 ID:SGTC_1647|Compound:st011722|FD-Score:4.08|P-value:1.19E-4 ID:SGTC_1716|Compound:st033144|FD-Score:-4.09|P-value:4.15E-4 ID:SGTC_1720|Compound:st037298|FD-Score:4.85|P-value:7.41E-6 ID:SGTC_1731|Compound:st035964|FD-Score:-6.28|P-value:8.56E-8 ID:SGTC_1733|Compound:st037357|FD-Score:-5.12|P-value:1.18E-5 ID:SGTC_1734|Compound:st037168|FD-Score:-5.58|P-value:1.89E-6 ID:SGTC_1775|Compound:st081588|FD-Score:-3.80|P-value:9.85E-4 ID:SGTC_1786|Compound:berberine|FD-Score:-6.16|P-value:1.52E-7 ID:SGTC_2015|Compound:5119600|FD-Score:3.65|P-value:4.81E-4 ID:SGTC_2166|Compound:5933061|FD-Score:3.83|P-value:2.71E-4 ID:SGTC_2205|Compound:7238770|FD-Score:3.44|P-value:9.09E-4 ID:SGTC_2209|Compound:5-(5-methyl-2-thenylidene)-Barbituric acid|FD-Score:3.65|P-value:4.73E-4 ID:SGTC_2346|Compound:9031566|FD-Score:6.83|P-value:7.94E-10 ID:SGTC_2399|Compound:5884133|FD-Score:4.78|P-value:9.81E-6 ID:SGTC_2414|Compound:st077232|FD-Score:4.20|P-value:8.02E-5 ID:SGTC_2439|Compound:5492030|FD-Score:3.47|P-value:8.10E-4 ID:SGTC_2445|Compound:5742597|FD-Score:3.41|P-value:9.67E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.46|P-value:8.41E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:-4.60|P-value:7.67E-5 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.55|P-value:6.55E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:-3.87|P-value:7.97E-4 ID:SGTC_2761|Compound:levamisole|FD-Score:4.63|P-value:1.71E-5 ID:SGTC_2766|Compound:oltipraz|FD-Score:-4.06|P-value:4.56E-4 ID:SGTC_2870|Compound:9038528|FD-Score:3.97|P-value:1.75E-4 ID:SGTC_2875|Compound:9039966|FD-Score:-3.87|P-value:8.06E-4 ID:SGTC_3075|Compound:9116269|FD-Score:5.82|P-value:1.20E-7 ID:SGTC_3286|Compound:9117135|FD-Score:5.17|P-value:2.01E-6

Top fitness defect scores for YMR146C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_756 0508-1999 11.40 28.3 µM 1.35E-23 RPP1 & pyrimidine depletion 1 2.54E-30 4.29
2 SGTC_1514 1189-1591 10.70 158.0 µM 4.33E-21 1 5.46E-27 5.94
3 SGTC_257 daunorubicin 9.98 18.5 µM 1.20E-18 anthracycline transcription coupled DNA repair 3 9.56E-24 1.03
4 SGTC_882 0843-0131 8.93 351.0 µM 2.29E-15 1 2.11E-19 2.34
5 SGTC_71 0417-1750 8.42 47.8 µM 6.97E-14 Golgi 1 1.92E-17 0.71
6 SGTC_2346 9031566 6.83 143.8 µM 7.94E-10 Golgi 5 4.19E-12 1.29
7 SGTC_894 0960-0238 6.75 55.9 µM 1.20E-9 4 7.18E-12 1.22
8 SGTC_3075 9116269 5.82 49.5 µM 1.20E-7 4 2.96E-9 0.39
9 SGTC_302 1094-0063 5.64 18.9 µM 2.73E-7 4 8.63E-9 0.33
10 SGTC_605 1315-0399 5.20 119.0 µM 1.82E-6 Golgi 3 1.01E-7 0.28
11 SGTC_3286 9117135 5.17 37.2 µM 2.01E-6 4 1.16E-7 0.44
12 SGTC_1134 3477-0009 5.04 42.5 µM 3.43E-6 6 2.30E-7 0.72
13 SGTC_1456 k015-0012 4.94 13.0 µM 5.18E-6 5 3.93E-7 0.71
14 SGTC_1720 st037298 4.85 9.5 µM 7.41E-6 4 6.24E-7 0.14
15 SGTC_2399 5884133 4.78 200.0 µM 9.81E-6 cell wall signaling 10 8.97E-7 0.06
16 SGTC_2761 levamisole 4.63 97.9 µM 1.71E-5 2 1.84E-6 0.61
17 SGTC_1537 2',6'-dihydroxyflavanone 4.61 78.0 µM 1.87E-5 1 2.06E-6 0.31
18 SGTC_1397 3532-0190 4.52 20.1 µM 2.58E-5 2 3.11E-6 1.26
19 SGTC_132 0417-1715 4.22 25.8 µM 7.50E-5 RPP1 & pyrimidine depletion 9 1.23E-5 0.27
20 SGTC_2414 st077232 4.20 92.9 µM 8.02E-5 4 1.34E-5 0.10

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.251 3.54E-49 YDR429C TIF35 het eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
0.175 1.33E-24 YIL143C SSL2 het Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3
0.152 8.55E-19 YPL238C_d YPL238C_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
0.137 1.63E-15 YPL237W SUI3 het Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
0.134 5.81E-15 YER025W GCD11 het Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met
0.124 6.50E-13 YPR136C_d YPR136C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
0.117 9.02E-12 YJR007W SUI2 het Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
0.113 4.94E-11 YKR059W TIF1 hom Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication
0.111 1.27E-10 YDR439W LRS4 hom Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation
0.110 2.09E-10 YPR137W RRP9 het Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
0.107 5.91E-10 YNL178W RPS3 het Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3
0.101 4.88E-9 YOR146W_d YOR146W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
0.100 5.57E-9 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.100 7.37E-9 YLR222C UTP13 het Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
0.099 8.35E-9 YGL123W RPS2 het Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5