HIPHOP chemogenomics database

YMR146C / TIF34

eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation

Zygosity: Heterozygous strain

fixedexpanded
Profile for YMR146C / TIF34

Click on Significant Values for Screen Details

Top fitness defect scores for YMR146C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_756	0508-1999	11.40	28.3 µM	1.35E-23	RPP1 & pyrimidine depletion	1	2.54E-30	4.29
2	SGTC_1514	1189-1591	10.70	158.0 µM	4.33E-21		1	5.46E-27	5.94
3	SGTC_257	daunorubicin	9.98	18.5 µM	1.20E-18	anthracycline transcription coupled DNA repair	3	9.56E-24	1.03
4	SGTC_882	0843-0131	8.93	351.0 µM	2.29E-15		1	2.11E-19	2.34
5	SGTC_71	0417-1750	8.42	47.8 µM	6.97E-14	Golgi	1	1.92E-17	0.71
6	SGTC_2346	9031566	6.83	143.8 µM	7.94E-10	Golgi	5	4.19E-12	1.29
7	SGTC_894	0960-0238	6.75	55.9 µM	1.20E-9		4	7.18E-12	1.22
8	SGTC_3075	9116269	5.82	49.5 µM	1.20E-7		4	2.96E-9	0.39
9	SGTC_302	1094-0063	5.64	18.9 µM	2.73E-7		4	8.63E-9	0.33
10	SGTC_605	1315-0399	5.20	119.0 µM	1.82E-6	Golgi	3	1.01E-7	0.28
11	SGTC_3286	9117135	5.17	37.2 µM	2.01E-6		4	1.16E-7	0.44
12	SGTC_1134	3477-0009	5.04	42.5 µM	3.43E-6		6	2.30E-7	0.72
13	SGTC_1456	k015-0012	4.94	13.0 µM	5.18E-6		5	3.93E-7	0.71
14	SGTC_1720	st037298	4.85	9.5 µM	7.41E-6		4	6.24E-7	0.14
15	SGTC_2399	5884133	4.78	200.0 µM	9.81E-6	cell wall signaling	10	8.97E-7	0.06
16	SGTC_2761	levamisole	4.63	97.9 µM	1.71E-5		2	1.84E-6	0.61
17	SGTC_1537	2',6'-dihydroxyflavanone	4.61	78.0 µM	1.87E-5		1	2.06E-6	0.31
18	SGTC_1397	3532-0190	4.52	20.1 µM	2.58E-5		2	3.11E-6	1.26
19	SGTC_132	0417-1715	4.22	25.8 µM	7.50E-5	RPP1 & pyrimidine depletion	9	1.23E-5	0.27
20	SGTC_2414	st077232	4.20	92.9 µM	8.02E-5		4	1.34E-5	0.10

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.251	3.54E-49	YDR429C	TIF35	het	eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
0.175	1.33E-24	YIL143C	SSL2	het	Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3
0.152	8.55E-19	YPL238C_d	YPL238C_d	het	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
0.137	1.63E-15	YPL237W	SUI3	het	Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
0.134	5.81E-15	YER025W	GCD11	het	Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met
0.124	6.50E-13	YPR136C_d	YPR136C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
0.117	9.02E-12	YJR007W	SUI2	het	Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
0.113	4.94E-11	YKR059W	TIF1	hom	Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication
0.111	1.27E-10	YDR439W	LRS4	hom	Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation
0.110	2.09E-10	YPR137W	RRP9	het	Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
0.107	5.91E-10	YNL178W	RPS3	het	Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3
0.101	4.88E-9	YOR146W_d	YOR146W_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
0.100	5.57E-9	YOR085W	OST3	hom	Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.100	7.37E-9	YLR222C	UTP13	het	Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
0.099	8.35E-9	YGL123W	RPS2	het	Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5