YMR159C / ATG16

Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR159C / ATG16

Click on Significant Values for Screen Details ID:SGTC_79|Compound:0052-0007|FD-Score:-3.07|P-value:9.69E-4 ID:SGTC_82|Compound:0054-0107|FD-Score:-3.82|P-value:5.85E-5 ID:SGTC_97|Compound:0535-0701|FD-Score:3.36|P-value:3.58E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:3.51|P-value:2.03E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:3.35|P-value:3.71E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:-3.29|P-value:4.40E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:3.41|P-value:2.94E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:4.96|P-value:2.79E-7 ID:SGTC_765|Compound:1319-0117|FD-Score:4.49|P-value:2.99E-6 ID:SGTC_808|Compound:0711-0032|FD-Score:3.98|P-value:3.02E-5 ID:SGTC_811|Compound:0416-0114|FD-Score:3.94|P-value:3.54E-5 ID:SGTC_1008|Compound:4106-0037|FD-Score:3.49|P-value:2.17E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:4.67|P-value:1.24E-6 ID:SGTC_1314|Compound:1222-0010|FD-Score:3.85|P-value:5.23E-5 ID:SGTC_1342|Compound:1486-1464|FD-Score:4.59|P-value:1.86E-6 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.46|P-value:2.47E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.51|P-value:2.04E-4 ID:SGTC_1526|Compound:st051518|FD-Score:3.92|P-value:3.90E-5 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:3.37|P-value:3.41E-4 ID:SGTC_1611|Compound:st001856|FD-Score:-3.28|P-value:4.66E-4 ID:SGTC_1612|Compound:st002233|FD-Score:4.32|P-value:6.78E-6 ID:SGTC_1624|Compound:st003710|FD-Score:5.54|P-value:1.19E-8 ID:SGTC_1627|Compound:st003713|FD-Score:3.17|P-value:6.99E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.33|P-value:3.96E-4 ID:SGTC_1843|Compound:st055809|FD-Score:3.47|P-value:2.33E-4 ID:SGTC_1847|Compound:st056231|FD-Score:4.05|P-value:2.20E-5 ID:SGTC_1856|Compound:st057054|FD-Score:3.92|P-value:3.81E-5 ID:SGTC_1923|Compound:st058458|FD-Score:5.08|P-value:1.55E-7 ID:SGTC_1950|Compound:st077106|FD-Score:3.89|P-value:4.36E-5 ID:SGTC_2029|Compound:5175081|FD-Score:3.90|P-value:4.20E-5 ID:SGTC_2290|Compound:7904846|FD-Score:4.33|P-value:6.43E-6 ID:SGTC_2336|Compound:9001819|FD-Score:6.28|P-value:1.23E-10 ID:SGTC_2339|Compound:9000433|FD-Score:3.38|P-value:3.25E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-3.10|P-value:8.86E-4 ID:SGTC_2481|Compound:5478948|FD-Score:5.98|P-value:8.23E-10 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:6.53|P-value:2.29E-11 ID:SGTC_2533|Compound:agelasine|FD-Score:7.09|P-value:4.41E-13 ID:SGTC_2534|Compound:helenine|FD-Score:-3.10|P-value:8.82E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:5.13|P-value:1.13E-7 ID:SGTC_2610|Compound:ononetin|FD-Score:3.29|P-value:4.61E-4 ID:SGTC_2680|Compound:butyl paraben|FD-Score:3.17|P-value:6.93E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:-4.31|P-value:6.73E-6 ID:SGTC_2832|Compound:7992762|FD-Score:-3.65|P-value:1.16E-4 ID:SGTC_2866|Compound:9034245|FD-Score:3.20|P-value:6.40E-4 ID:SGTC_2887|Compound:9044784|FD-Score:3.45|P-value:2.49E-4 ID:SGTC_3033|Compound:9090887|FD-Score:5.48|P-value:1.62E-8 ID:SGTC_3133|Compound:9127736|FD-Score:3.96|P-value:3.23E-5 ID:SGTC_3178|Compound:9107506|FD-Score:3.07|P-value:9.82E-4 ID:SGTC_3224|Compound:9130643|FD-Score:3.22|P-value:5.76E-4 ID:SGTC_79|Compound:0052-0007|FD-Score:-3.07|P-value:9.69E-4 ID:SGTC_82|Compound:0054-0107|FD-Score:-3.82|P-value:5.85E-5 ID:SGTC_97|Compound:0535-0701|FD-Score:3.36|P-value:3.58E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:3.51|P-value:2.03E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:3.35|P-value:3.71E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:-3.29|P-value:4.40E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:3.41|P-value:2.94E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:4.96|P-value:2.79E-7 ID:SGTC_765|Compound:1319-0117|FD-Score:4.49|P-value:2.99E-6 ID:SGTC_808|Compound:0711-0032|FD-Score:3.98|P-value:3.02E-5 ID:SGTC_811|Compound:0416-0114|FD-Score:3.94|P-value:3.54E-5 ID:SGTC_1008|Compound:4106-0037|FD-Score:3.49|P-value:2.17E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:4.67|P-value:1.24E-6 ID:SGTC_1314|Compound:1222-0010|FD-Score:3.85|P-value:5.23E-5 ID:SGTC_1342|Compound:1486-1464|FD-Score:4.59|P-value:1.86E-6 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.46|P-value:2.47E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.51|P-value:2.04E-4 ID:SGTC_1526|Compound:st051518|FD-Score:3.92|P-value:3.90E-5 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:3.37|P-value:3.41E-4 ID:SGTC_1611|Compound:st001856|FD-Score:-3.28|P-value:4.66E-4 ID:SGTC_1612|Compound:st002233|FD-Score:4.32|P-value:6.78E-6 ID:SGTC_1624|Compound:st003710|FD-Score:5.54|P-value:1.19E-8 ID:SGTC_1627|Compound:st003713|FD-Score:3.17|P-value:6.99E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.33|P-value:3.96E-4 ID:SGTC_1843|Compound:st055809|FD-Score:3.47|P-value:2.33E-4 ID:SGTC_1847|Compound:st056231|FD-Score:4.05|P-value:2.20E-5 ID:SGTC_1856|Compound:st057054|FD-Score:3.92|P-value:3.81E-5 ID:SGTC_1923|Compound:st058458|FD-Score:5.08|P-value:1.55E-7 ID:SGTC_1950|Compound:st077106|FD-Score:3.89|P-value:4.36E-5 ID:SGTC_2029|Compound:5175081|FD-Score:3.90|P-value:4.20E-5 ID:SGTC_2290|Compound:7904846|FD-Score:4.33|P-value:6.43E-6 ID:SGTC_2336|Compound:9001819|FD-Score:6.28|P-value:1.23E-10 ID:SGTC_2339|Compound:9000433|FD-Score:3.38|P-value:3.25E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-3.10|P-value:8.86E-4 ID:SGTC_2481|Compound:5478948|FD-Score:5.98|P-value:8.23E-10 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:6.53|P-value:2.29E-11 ID:SGTC_2533|Compound:agelasine|FD-Score:7.09|P-value:4.41E-13 ID:SGTC_2534|Compound:helenine|FD-Score:-3.10|P-value:8.82E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:5.13|P-value:1.13E-7 ID:SGTC_2610|Compound:ononetin|FD-Score:3.29|P-value:4.61E-4 ID:SGTC_2680|Compound:butyl paraben|FD-Score:3.17|P-value:6.93E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:-4.31|P-value:6.73E-6 ID:SGTC_2832|Compound:7992762|FD-Score:-3.65|P-value:1.16E-4 ID:SGTC_2866|Compound:9034245|FD-Score:3.20|P-value:6.40E-4 ID:SGTC_2887|Compound:9044784|FD-Score:3.45|P-value:2.49E-4 ID:SGTC_3033|Compound:9090887|FD-Score:5.48|P-value:1.62E-8 ID:SGTC_3133|Compound:9127736|FD-Score:3.96|P-value:3.23E-5 ID:SGTC_3178|Compound:9107506|FD-Score:3.07|P-value:9.82E-4 ID:SGTC_3224|Compound:9130643|FD-Score:3.22|P-value:5.76E-4

Top fitness defect scores for YMR159C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2533 agelasine 7.09 13.7 µM 4.41E-13 ERG2 7 6.78E-13
2 SGTC_2531 methyl deoxycholate 6.53 70.1 µM 2.29E-11 5 3.30E-11
3 SGTC_2336 9001819 6.28 198.7 µM 1.23E-10 heme biosynthesis & mitochondrial translocase 5 1.72E-10
4 SGTC_2481 5478948 5.98 45.2 µM 8.23E-10 PDR1 9 1.12E-9
5 SGTC_1624 st003710 5.54 6.2 µM 1.19E-8 calcium & mitochondrial duress 17 1.54E-8
6 SGTC_3033 9090887 5.48 49.5 µM 1.62E-8 fatty acid desaturase (OLE1) 6 2.10E-8
7 SGTC_2590 3,4',5',6',7'-pentamethoxyflavone 5.13 100.0 µM 1.13E-7 60S ribosome export 8 1.42E-7
8 SGTC_1923 st058458 5.08 19.1 µM 1.55E-7 13 1.94E-7
9 SGTC_621 3584-0052 4.96 32.1 µM 2.79E-7 14 3.44E-7
10 SGTC_1025 k007-0731 4.67 70.0 µM 1.24E-6 6 1.50E-6
11 SGTC_1342 1486-1464 4.59 234.0 µM 1.86E-6 5 2.22E-6
12 SGTC_765 1319-0117 4.49 244.0 µM 2.99E-6 60S ribosome export 5 3.56E-6
13 SGTC_2290 7904846 4.33 200.0 µM 6.43E-6 13 7.55E-6
14 SGTC_1612 st002233 4.32 45.4 µM 6.78E-6 10 7.95E-6
15 SGTC_1847 st056231 4.05 15.0 µM 2.20E-5 36 2.53E-5
16 SGTC_808 0711-0032 3.98 135.0 µM 3.02E-5 12 3.46E-5
17 SGTC_3133 9127736 3.96 49.5 µM 3.23E-5 16 3.69E-5
18 SGTC_811 0416-0114 3.94 265.0 µM 3.54E-5 19 4.04E-5
19 SGTC_1856 st057054 3.92 55.7 µM 3.81E-5 29 4.34E-5
20 SGTC_1526 st051518 3.92 75.0 µM 3.90E-5 21 4.44E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.122 1.07E-12 YIL078W THS1 het Threonyl-tRNA synthetase, essential cytoplasmic protein
0.104 1.61E-9 YJR076C CDC11 het Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
0.100 5.82E-9 YHR049W FSH1 hom Putative serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2
0.094 5.55E-8 YDR294C DPL1 hom Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate
0.085 7.16E-7 YDR419W RAD30 hom DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV
0.084 1.18E-6 YPL271W ATP15 hom Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
0.084 1.11E-6 YDL154W MSH5 hom Protein of the MutS family, forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans
0.084 1.17E-6 YMR089C YTA12 hom Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes
0.081 2.62E-6 YOL055C THI20 hom Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p
0.079 4.17E-6 YOR266W PNT1 hom Mitochondrial integral inner membrane protein involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine
0.079 4.29E-6 YER028C MIG3 hom Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication
0.077 7.60E-6 YLL047W_d YLL047W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1
0.076 1.03E-5 YOL083W ATG34 hom Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog
0.076 1.12E-5 YMR242W-A_p YMR242W-A_p hom Putative protein of unknown function
0.076 1.12E-5 YLR224W_p YLR224W_p hom F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene