YMR171C / EAR1

Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR171C / EAR1

Click on Significant Values for Screen Details ID:SGTC_350|Compound:k064-0020|FD-Score:-3.09|P-value:6.28E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:3.38|P-value:2.56E-4 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:-3.83|P-value:3.27E-5 ID:SGTC_512|Compound:prima-1|FD-Score:3.15|P-value:6.06E-4 ID:SGTC_658|Compound:3253-0951|FD-Score:3.44|P-value:2.03E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:3.22|P-value:4.74E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:3.25|P-value:4.16E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:3.80|P-value:4.53E-5 ID:SGTC_961|Compound:1252-1571|FD-Score:-3.39|P-value:2.03E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:-3.07|P-value:6.89E-4 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.28|P-value:3.71E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:-3.82|P-value:3.45E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:4.77|P-value:4.54E-7 ID:SGTC_1068|Compound:loratadine|FD-Score:3.19|P-value:5.23E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.92|P-value:2.76E-5 ID:SGTC_1084|Compound:voriconazole|FD-Score:3.05|P-value:8.49E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:3.18|P-value:5.43E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.26|P-value:4.02E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.44|P-value:2.01E-4 ID:SGTC_1690|Compound:st023502|FD-Score:4.17|P-value:8.83E-6 ID:SGTC_1748|Compound:st037455|FD-Score:3.67|P-value:7.93E-5 ID:SGTC_1761|Compound:st044296|FD-Score:-3.14|P-value:5.24E-4 ID:SGTC_1765|Compound:st044815|FD-Score:-5.33|P-value:1.49E-8 ID:SGTC_1818|Compound:st052136|FD-Score:3.67|P-value:7.97E-5 ID:SGTC_1864|Compound:st057645|FD-Score:3.85|P-value:3.74E-5 ID:SGTC_1866|Compound:st057638|FD-Score:-3.49|P-value:1.40E-4 ID:SGTC_1943|Compound:st076331|FD-Score:3.47|P-value:1.79E-4 ID:SGTC_2085|Compound:5307070|FD-Score:3.14|P-value:6.31E-4 ID:SGTC_2116|Compound:5511452|FD-Score:3.34|P-value:2.97E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:-3.39|P-value:2.03E-4 ID:SGTC_2674|Compound:ligustilide|FD-Score:4.49|P-value:1.89E-6 ID:SGTC_2807|Compound:7945238|FD-Score:3.25|P-value:4.12E-4 ID:SGTC_2814|Compound:7977482|FD-Score:7.74|P-value:7.00E-16 ID:SGTC_2981|Compound:9013952|FD-Score:3.10|P-value:7.21E-4 ID:SGTC_3019|Compound:9081971|FD-Score:3.10|P-value:7.20E-4 ID:SGTC_3217|Compound:9129673|FD-Score:3.73|P-value:6.25E-5 ID:SGTC_3286|Compound:9117135|FD-Score:5.87|P-value:7.04E-10 ID:SGTC_3311|Compound:9131146|FD-Score:3.04|P-value:8.87E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:-3.09|P-value:6.28E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:3.38|P-value:2.56E-4 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:-3.83|P-value:3.27E-5 ID:SGTC_512|Compound:prima-1|FD-Score:3.15|P-value:6.06E-4 ID:SGTC_658|Compound:3253-0951|FD-Score:3.44|P-value:2.03E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:3.22|P-value:4.74E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:3.25|P-value:4.16E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:3.80|P-value:4.53E-5 ID:SGTC_961|Compound:1252-1571|FD-Score:-3.39|P-value:2.03E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:-3.07|P-value:6.89E-4 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.28|P-value:3.71E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:-3.82|P-value:3.45E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:4.77|P-value:4.54E-7 ID:SGTC_1068|Compound:loratadine|FD-Score:3.19|P-value:5.23E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.92|P-value:2.76E-5 ID:SGTC_1084|Compound:voriconazole|FD-Score:3.05|P-value:8.49E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:3.18|P-value:5.43E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.26|P-value:4.02E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.44|P-value:2.01E-4 ID:SGTC_1690|Compound:st023502|FD-Score:4.17|P-value:8.83E-6 ID:SGTC_1748|Compound:st037455|FD-Score:3.67|P-value:7.93E-5 ID:SGTC_1761|Compound:st044296|FD-Score:-3.14|P-value:5.24E-4 ID:SGTC_1765|Compound:st044815|FD-Score:-5.33|P-value:1.49E-8 ID:SGTC_1818|Compound:st052136|FD-Score:3.67|P-value:7.97E-5 ID:SGTC_1864|Compound:st057645|FD-Score:3.85|P-value:3.74E-5 ID:SGTC_1866|Compound:st057638|FD-Score:-3.49|P-value:1.40E-4 ID:SGTC_1943|Compound:st076331|FD-Score:3.47|P-value:1.79E-4 ID:SGTC_2085|Compound:5307070|FD-Score:3.14|P-value:6.31E-4 ID:SGTC_2116|Compound:5511452|FD-Score:3.34|P-value:2.97E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:-3.39|P-value:2.03E-4 ID:SGTC_2674|Compound:ligustilide|FD-Score:4.49|P-value:1.89E-6 ID:SGTC_2807|Compound:7945238|FD-Score:3.25|P-value:4.12E-4 ID:SGTC_2814|Compound:7977482|FD-Score:7.74|P-value:7.00E-16 ID:SGTC_2981|Compound:9013952|FD-Score:3.10|P-value:7.21E-4 ID:SGTC_3019|Compound:9081971|FD-Score:3.10|P-value:7.20E-4 ID:SGTC_3217|Compound:9129673|FD-Score:3.73|P-value:6.25E-5 ID:SGTC_3286|Compound:9117135|FD-Score:5.87|P-value:7.04E-10 ID:SGTC_3311|Compound:9131146|FD-Score:3.04|P-value:8.87E-4

Top fitness defect scores for YMR171C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2814 7977482 7.74 43.0 µM 7.00E-16 4 5.07E-15
2 SGTC_3286 9117135 5.87 37.2 µM 7.04E-10 2 2.15E-9
3 SGTC_1044 1222-0021 4.77 1.8 µM 4.54E-7 heme biosynthesis & mitochondrial translocase 26 9.35E-7
4 SGTC_2674 ligustilide 4.49 100.0 µM 1.89E-6 3 3.57E-6
5 SGTC_1690 st023502 4.17 41.2 µM 8.83E-6 calcium & mitochondrial duress 7 1.52E-5
6 SGTC_1072 oxiconazole 3.92 14.9 nM 2.76E-5 ergosterol depletion effects on membrane 55 4.46E-5
7 SGTC_1864 st057645 3.85 30.4 µM 3.74E-5 amide catabolism 6 5.93E-5
8 SGTC_927 2425-1185 3.80 123.0 µM 4.53E-5 29 7.10E-5
9 SGTC_3217 9129673 3.73 49.5 µM 6.25E-5 46 9.62E-5
10 SGTC_1748 st037455 3.67 53.6 µM 7.93E-5 39 1.20E-4
11 SGTC_1818 st052136 3.67 56.3 µM 7.97E-5 NEO1-PIK1 67 1.21E-4
12 SGTC_1943 st076331 3.47 65.2 µM 1.79E-4 27 2.59E-4
13 SGTC_1305 1181-0477 3.44 144.0 µM 2.01E-4 30 2.90E-4
14 SGTC_658 3253-0951 3.44 102.0 µM 2.03E-4 calcium & mitochondrial duress 84 2.91E-4
15 SGTC_403 arphamenine a 3.38 50.0 µM 2.56E-4 60S ribosome export 30 3.64E-4
16 SGTC_2116 5511452 3.34 16.9 µM 2.97E-4 34 4.19E-4
17 SGTC_1035 k081-0041 3.28 55.2 µM 3.71E-4 Golgi 44 5.16E-4
18 SGTC_1259 0671-0132 3.26 43.0 µM 4.02E-4 55 5.56E-4
19 SGTC_2807 7945238 3.25 52.0 µM 4.12E-4 63 5.69E-4
20 SGTC_894 0960-0238 3.25 55.9 µM 4.16E-4 28 5.75E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.216 1.03E-36 YLR097C HRT3 hom Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies
0.170 3.40E-23 YOR060C SLD7 het Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria
0.168 1.23E-22 YDR020C_p DAS2_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases
0.118 6.70E-12 YOR268C_p YOR268C_p hom Putative protein of unknown function; sporulation is abnormal in homozygous diploid; YOR268C is not an essential gene
0.115 2.63E-11 YKL092C BUD2 hom GTPase activating factor for Rsr1p/Bud1p; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; mutant deficient in mitotic spindle position checkpoint
0.105 1.04E-9 YOR329C SCD5 het Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
0.100 7.34E-9 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.097 1.97E-8 YGR142W BTN2 hom v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication
0.093 6.86E-8 YBR214W SDS24 hom Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication
0.090 1.55E-7 YKL097C_d YKL097C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species
0.089 2.20E-7 YDR468C TLG1 het Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p
0.087 5.12E-7 YDR502C SAM2 hom S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication
0.082 2.15E-6 YBL073W_d YBL073W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C
0.081 2.50E-6 YJL042W MHP1 hom Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins
0.081 2.93E-6 YJL029C VPS53 hom Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting