YMR172W / HOT1

Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR172W / HOT1

Click on Significant Values for Screen Details ID:SGTC_371|Compound:0195-0006|FD-Score:4.60|P-value:2.56E-7 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:2.88|P-value:7.98E-4 ID:SGTC_480|Compound:am 92016|FD-Score:3.18|P-value:2.56E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:3.04|P-value:4.42E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:-2.87|P-value:9.64E-4 ID:SGTC_849|Compound:0386-0493|FD-Score:-3.03|P-value:5.33E-4 ID:SGTC_859|Compound:0439-0102|FD-Score:2.99|P-value:5.23E-4 ID:SGTC_861|Compound:0443-0273|FD-Score:4.59|P-value:2.66E-7 ID:SGTC_983|Compound:1491-0821|FD-Score:3.03|P-value:4.53E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.14|P-value:2.93E-4 ID:SGTC_1324|Compound:1418-0026|FD-Score:3.59|P-value:4.43E-5 ID:SGTC_1328|Compound:1441-0025|FD-Score:-4.26|P-value:2.06E-6 ID:SGTC_1330|Compound:1469-0054|FD-Score:-3.26|P-value:2.18E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:-4.50|P-value:5.67E-7 ID:SGTC_1333|Compound:1482-0047|FD-Score:-3.88|P-value:1.36E-5 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.02|P-value:4.81E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:-2.93|P-value:7.87E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:-3.17|P-value:3.14E-4 ID:SGTC_1625|Compound:st003711|FD-Score:3.37|P-value:1.13E-4 ID:SGTC_1716|Compound:st033144|FD-Score:3.03|P-value:4.64E-4 ID:SGTC_1795|Compound:st049545|FD-Score:3.99|P-value:6.64E-6 ID:SGTC_2081|Compound:5214970|FD-Score:3.44|P-value:8.51E-5 ID:SGTC_2090|Compound:5301565|FD-Score:-3.21|P-value:2.59E-4 ID:SGTC_2286|Compound:7945697|FD-Score:4.00|P-value:6.29E-6 ID:SGTC_2332|Compound:7982454|FD-Score:-4.59|P-value:3.32E-7 ID:SGTC_2389|Compound:9091542|FD-Score:3.01|P-value:4.94E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:3.17|P-value:2.66E-4 ID:SGTC_2517|Compound:totarol|FD-Score:3.44|P-value:8.46E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.68|P-value:2.89E-5 ID:SGTC_2550|Compound:chrysin dimethyl ether|FD-Score:4.71|P-value:1.35E-7 ID:SGTC_2551|Compound:apiole|FD-Score:3.22|P-value:2.16E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:-3.98|P-value:8.15E-6 ID:SGTC_2561|Compound:acetosyringone|FD-Score:-4.05|P-value:5.91E-6 ID:SGTC_2729|Compound:fluphenazine|FD-Score:-3.60|P-value:5.03E-5 ID:SGTC_2832|Compound:7992762|FD-Score:-5.14|P-value:1.32E-8 ID:SGTC_2835|Compound:9006588|FD-Score:6.18|P-value:7.71E-12 ID:SGTC_2849|Compound:9019321|FD-Score:2.99|P-value:5.35E-4 ID:SGTC_2882|Compound:9058044|FD-Score:3.20|P-value:2.36E-4 ID:SGTC_2908|Compound:7930301|FD-Score:-5.05|P-value:2.25E-8 ID:SGTC_2974|Compound:9094440|FD-Score:-3.17|P-value:3.05E-4 ID:SGTC_2978|Compound:9011784|FD-Score:3.00|P-value:5.22E-4 ID:SGTC_3085|Compound:9117376|FD-Score:2.89|P-value:7.72E-4 ID:SGTC_3159|Compound:9100096|FD-Score:-4.07|P-value:5.32E-6 ID:SGTC_3206|Compound:9114350|FD-Score:3.01|P-value:5.00E-4 ID:SGTC_3239|Compound:9133757|FD-Score:2.84|P-value:9.44E-4 ID:SGTC_3242|Compound:9133940|FD-Score:3.87|P-value:1.20E-5 ID:SGTC_3246|Compound:9135460|FD-Score:3.74|P-value:2.15E-5 ID:SGTC_371|Compound:0195-0006|FD-Score:4.60|P-value:2.56E-7 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:2.88|P-value:7.98E-4 ID:SGTC_480|Compound:am 92016|FD-Score:3.18|P-value:2.56E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:3.04|P-value:4.42E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:-2.87|P-value:9.64E-4 ID:SGTC_849|Compound:0386-0493|FD-Score:-3.03|P-value:5.33E-4 ID:SGTC_859|Compound:0439-0102|FD-Score:2.99|P-value:5.23E-4 ID:SGTC_861|Compound:0443-0273|FD-Score:4.59|P-value:2.66E-7 ID:SGTC_983|Compound:1491-0821|FD-Score:3.03|P-value:4.53E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.14|P-value:2.93E-4 ID:SGTC_1324|Compound:1418-0026|FD-Score:3.59|P-value:4.43E-5 ID:SGTC_1328|Compound:1441-0025|FD-Score:-4.26|P-value:2.06E-6 ID:SGTC_1330|Compound:1469-0054|FD-Score:-3.26|P-value:2.18E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:-4.50|P-value:5.67E-7 ID:SGTC_1333|Compound:1482-0047|FD-Score:-3.88|P-value:1.36E-5 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.02|P-value:4.81E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:-2.93|P-value:7.87E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:-3.17|P-value:3.14E-4 ID:SGTC_1625|Compound:st003711|FD-Score:3.37|P-value:1.13E-4 ID:SGTC_1716|Compound:st033144|FD-Score:3.03|P-value:4.64E-4 ID:SGTC_1795|Compound:st049545|FD-Score:3.99|P-value:6.64E-6 ID:SGTC_2081|Compound:5214970|FD-Score:3.44|P-value:8.51E-5 ID:SGTC_2090|Compound:5301565|FD-Score:-3.21|P-value:2.59E-4 ID:SGTC_2286|Compound:7945697|FD-Score:4.00|P-value:6.29E-6 ID:SGTC_2332|Compound:7982454|FD-Score:-4.59|P-value:3.32E-7 ID:SGTC_2389|Compound:9091542|FD-Score:3.01|P-value:4.94E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:3.17|P-value:2.66E-4 ID:SGTC_2517|Compound:totarol|FD-Score:3.44|P-value:8.46E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.68|P-value:2.89E-5 ID:SGTC_2550|Compound:chrysin dimethyl ether|FD-Score:4.71|P-value:1.35E-7 ID:SGTC_2551|Compound:apiole|FD-Score:3.22|P-value:2.16E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:-3.98|P-value:8.15E-6 ID:SGTC_2561|Compound:acetosyringone|FD-Score:-4.05|P-value:5.91E-6 ID:SGTC_2729|Compound:fluphenazine|FD-Score:-3.60|P-value:5.03E-5 ID:SGTC_2832|Compound:7992762|FD-Score:-5.14|P-value:1.32E-8 ID:SGTC_2835|Compound:9006588|FD-Score:6.18|P-value:7.71E-12 ID:SGTC_2849|Compound:9019321|FD-Score:2.99|P-value:5.35E-4 ID:SGTC_2882|Compound:9058044|FD-Score:3.20|P-value:2.36E-4 ID:SGTC_2908|Compound:7930301|FD-Score:-5.05|P-value:2.25E-8 ID:SGTC_2974|Compound:9094440|FD-Score:-3.17|P-value:3.05E-4 ID:SGTC_2978|Compound:9011784|FD-Score:3.00|P-value:5.22E-4 ID:SGTC_3085|Compound:9117376|FD-Score:2.89|P-value:7.72E-4 ID:SGTC_3159|Compound:9100096|FD-Score:-4.07|P-value:5.32E-6 ID:SGTC_3206|Compound:9114350|FD-Score:3.01|P-value:5.00E-4 ID:SGTC_3239|Compound:9133757|FD-Score:2.84|P-value:9.44E-4 ID:SGTC_3242|Compound:9133940|FD-Score:3.87|P-value:1.20E-5 ID:SGTC_3246|Compound:9135460|FD-Score:3.74|P-value:2.15E-5

Top fitness defect scores for YMR172W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_2835 9006588 6.18 71.4 µM 7.71E-12 6 3.14E-10
2 SGTC_2550 chrysin dimethyl ether 4.71 71.9 µM 1.35E-7 4 1.23E-6
3 SGTC_371 0195-0006 4.60 7.4 µM 2.56E-7 10 2.13E-6
4 SGTC_861 0443-0273 4.59 110.0 µM 2.66E-7 5 2.19E-6
5 SGTC_2286 7945697 4.00 125.0 µM 6.29E-6 55 3.21E-5
6 SGTC_1795 st049545 3.99 62.3 µM 6.64E-6 TSC3-RPN4 10 3.36E-5
7 SGTC_3242 9133940 3.87 49.5 µM 1.20E-5 13 5.54E-5
8 SGTC_3246 9135460 3.74 33.0 µM 2.15E-5 7 9.09E-5
9 SGTC_2519 cryptotanshinone 3.68 88.0 µM 2.89E-5 heme biosynthesis & mitochondrial translocase 20 1.17E-4
10 SGTC_1324 1418-0026 3.59 34.4 µM 4.43E-5 19 1.68E-4
11 SGTC_2517 totarol 3.44 10.0 µM 8.46E-5 35 2.91E-4
12 SGTC_2081 5214970 3.44 32.3 µM 8.51E-5 39 2.93E-4
13 SGTC_1625 st003711 3.37 30.1 µM 1.13E-4 calcium & mitochondrial duress 60 3.73E-4
14 SGTC_2551 apiole 3.22 73.9 µM 2.16E-4 25 6.48E-4
15 SGTC_2882 9058044 3.20 58.4 µM 2.36E-4 37 6.98E-4
16 SGTC_480 am 92016 3.18 50.0 µM 2.56E-4 44 7.48E-4
17 SGTC_2509 methyl 7-deshydroxypyrogallin-4-carboxylate 3.17 54.6 µM 2.66E-4 RPP1 & pyrimidine depletion 56 7.72E-4
18 SGTC_1229 0139-0193 3.14 12.2 µM 2.93E-4 RSC complex & mRNA processing 41 8.37E-4
19 SGTC_554 4483-2211 3.04 187.0 µM 4.42E-4 50 0.00119
20 SGTC_983 1491-0821 3.03 12.2 µM 4.53E-4 42 0.00121

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.116 1.59E-11 YDR056C_p YDR056C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein
0.094 4.50E-8 YPL087W YDC1 hom Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication
0.091 1.35E-7 YOR057W SGT1 het Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress
0.086 7.00E-7 YGL001C ERG26 het C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
0.084 1.10E-6 YBL098W BNA4 hom Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease
0.083 1.70E-6 YEL008W_d YEL008W_d hom Hypothetical protein predicted to be involved in metabolism
0.082 2.01E-6 YKR036C CAF4 hom WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication
0.079 4.87E-6 YOL007C CSI2 hom Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene
0.078 5.75E-6 YPR129W SCD6 hom Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress
0.078 6.46E-6 YHL021C AIM17 hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss
0.077 7.75E-6 YLR056W ERG3 hom C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase
0.076 9.66E-6 YDR314C RAD34 hom Protein involved in nucleotide excision repair (NER); homologous to RAD4
0.076 1.11E-5 YIL101C XBP1 hom Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
0.075 1.25E-5 YJL215C_d YJL215C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.074 1.72E-5 YMR009W ADI1 hom Acireductone dioxygenease involved in the methionine salvage pathway; ortholog of human MTCBP-1; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions