YMR176W / ECM5

Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR176W / ECM5

Click on Significant Values for Screen Details ID:SGTC_190|Compound:3970-0790|FD-Score:-6.18|P-value:5.35E-10 ID:SGTC_208|Compound:k072-0098|FD-Score:-3.34|P-value:5.13E-4 ID:SGTC_211|Compound:3455-0679|FD-Score:3.83|P-value:6.50E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:5.46|P-value:2.69E-8 ID:SGTC_368|Compound:fentichlor|FD-Score:4.71|P-value:1.33E-6 ID:SGTC_534|Compound:k292-0788|FD-Score:4.39|P-value:6.03E-6 ID:SGTC_538|Compound:2922-0838|FD-Score:3.65|P-value:1.33E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:3.71|P-value:1.05E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:3.47|P-value:2.59E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:3.80|P-value:7.42E-5 ID:SGTC_921|Compound:1561-0023|FD-Score:-3.24|P-value:7.24E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:4.31|P-value:8.49E-6 ID:SGTC_1053|Compound:3366-3204|FD-Score:-3.33|P-value:5.27E-4 ID:SGTC_1112|Compound:tnp00069|FD-Score:4.56|P-value:2.73E-6 ID:SGTC_1115|Compound:galangin|FD-Score:4.40|P-value:5.81E-6 ID:SGTC_1122|Compound:capsaicin|FD-Score:3.21|P-value:6.64E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-5.13|P-value:2.11E-7 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.51|P-value:2.23E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:3.69|P-value:1.16E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:-3.83|P-value:8.26E-5 ID:SGTC_1569|Compound:carminic acid|FD-Score:-3.74|P-value:1.18E-4 ID:SGTC_1633|Compound:st005664|FD-Score:3.43|P-value:3.09E-4 ID:SGTC_1650|Compound:st011916|FD-Score:4.07|P-value:2.41E-5 ID:SGTC_1708|Compound:st033234|FD-Score:3.10|P-value:9.63E-4 ID:SGTC_1717|Compound:st033143|FD-Score:3.44|P-value:2.92E-4 ID:SGTC_2002|Compound:4023503|FD-Score:-6.03|P-value:1.32E-9 ID:SGTC_2009|Compound:4012297|FD-Score:-5.30|P-value:8.47E-8 ID:SGTC_2066|Compound:5222451|FD-Score:4.20|P-value:1.38E-5 ID:SGTC_2071|Compound:5230951|FD-Score:-3.80|P-value:9.28E-5 ID:SGTC_2260|Compound:7964312|FD-Score:-3.49|P-value:3.02E-4 ID:SGTC_2284|Compound:7945075|FD-Score:4.30|P-value:9.08E-6 ID:SGTC_2290|Compound:7904846|FD-Score:3.53|P-value:2.13E-4 ID:SGTC_2301|Compound:7769476|FD-Score:-3.81|P-value:8.79E-5 ID:SGTC_2322|Compound:7413319|FD-Score:3.60|P-value:1.59E-4 ID:SGTC_2443|Compound:5493264|FD-Score:3.55|P-value:1.94E-4 ID:SGTC_2492|Compound:derrusnin|FD-Score:3.13|P-value:8.74E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:-5.69|P-value:1.01E-8 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:3.40|P-value:3.42E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:-3.32|P-value:5.55E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:4.40|P-value:5.81E-6 ID:SGTC_2661|Compound:dactinomycin|FD-Score:-3.70|P-value:1.36E-4 ID:SGTC_2662|Compound:daunorubicin|FD-Score:3.25|P-value:5.82E-4 ID:SGTC_2671|Compound:danthron|FD-Score:4.04|P-value:2.75E-5 ID:SGTC_2682|Compound:pyrogallin|FD-Score:-3.46|P-value:3.38E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:-3.26|P-value:6.82E-4 ID:SGTC_2841|Compound:9004761|FD-Score:3.86|P-value:5.88E-5 ID:SGTC_2903|Compound:5923464|FD-Score:-3.15|P-value:9.96E-4 ID:SGTC_2906|Compound:7926292|FD-Score:-3.30|P-value:5.94E-4 ID:SGTC_2916|Compound:7875993|FD-Score:3.11|P-value:9.44E-4 ID:SGTC_190|Compound:3970-0790|FD-Score:-6.18|P-value:5.35E-10 ID:SGTC_208|Compound:k072-0098|FD-Score:-3.34|P-value:5.13E-4 ID:SGTC_211|Compound:3455-0679|FD-Score:3.83|P-value:6.50E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:5.46|P-value:2.69E-8 ID:SGTC_368|Compound:fentichlor|FD-Score:4.71|P-value:1.33E-6 ID:SGTC_534|Compound:k292-0788|FD-Score:4.39|P-value:6.03E-6 ID:SGTC_538|Compound:2922-0838|FD-Score:3.65|P-value:1.33E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:3.71|P-value:1.05E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:3.47|P-value:2.59E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:3.80|P-value:7.42E-5 ID:SGTC_921|Compound:1561-0023|FD-Score:-3.24|P-value:7.24E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:4.31|P-value:8.49E-6 ID:SGTC_1053|Compound:3366-3204|FD-Score:-3.33|P-value:5.27E-4 ID:SGTC_1112|Compound:tnp00069|FD-Score:4.56|P-value:2.73E-6 ID:SGTC_1115|Compound:galangin|FD-Score:4.40|P-value:5.81E-6 ID:SGTC_1122|Compound:capsaicin|FD-Score:3.21|P-value:6.64E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:-5.13|P-value:2.11E-7 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.51|P-value:2.23E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:3.69|P-value:1.16E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:-3.83|P-value:8.26E-5 ID:SGTC_1569|Compound:carminic acid|FD-Score:-3.74|P-value:1.18E-4 ID:SGTC_1633|Compound:st005664|FD-Score:3.43|P-value:3.09E-4 ID:SGTC_1650|Compound:st011916|FD-Score:4.07|P-value:2.41E-5 ID:SGTC_1708|Compound:st033234|FD-Score:3.10|P-value:9.63E-4 ID:SGTC_1717|Compound:st033143|FD-Score:3.44|P-value:2.92E-4 ID:SGTC_2002|Compound:4023503|FD-Score:-6.03|P-value:1.32E-9 ID:SGTC_2009|Compound:4012297|FD-Score:-5.30|P-value:8.47E-8 ID:SGTC_2066|Compound:5222451|FD-Score:4.20|P-value:1.38E-5 ID:SGTC_2071|Compound:5230951|FD-Score:-3.80|P-value:9.28E-5 ID:SGTC_2260|Compound:7964312|FD-Score:-3.49|P-value:3.02E-4 ID:SGTC_2284|Compound:7945075|FD-Score:4.30|P-value:9.08E-6 ID:SGTC_2290|Compound:7904846|FD-Score:3.53|P-value:2.13E-4 ID:SGTC_2301|Compound:7769476|FD-Score:-3.81|P-value:8.79E-5 ID:SGTC_2322|Compound:7413319|FD-Score:3.60|P-value:1.59E-4 ID:SGTC_2443|Compound:5493264|FD-Score:3.55|P-value:1.94E-4 ID:SGTC_2492|Compound:derrusnin|FD-Score:3.13|P-value:8.74E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:-5.69|P-value:1.01E-8 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:3.40|P-value:3.42E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:-3.32|P-value:5.55E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:4.40|P-value:5.81E-6 ID:SGTC_2661|Compound:dactinomycin|FD-Score:-3.70|P-value:1.36E-4 ID:SGTC_2662|Compound:daunorubicin|FD-Score:3.25|P-value:5.82E-4 ID:SGTC_2671|Compound:danthron|FD-Score:4.04|P-value:2.75E-5 ID:SGTC_2682|Compound:pyrogallin|FD-Score:-3.46|P-value:3.38E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:-3.26|P-value:6.82E-4 ID:SGTC_2841|Compound:9004761|FD-Score:3.86|P-value:5.88E-5 ID:SGTC_2903|Compound:5923464|FD-Score:-3.15|P-value:9.96E-4 ID:SGTC_2906|Compound:7926292|FD-Score:-3.30|P-value:5.94E-4 ID:SGTC_2916|Compound:7875993|FD-Score:3.11|P-value:9.44E-4

Top fitness defect scores for YMR176W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_362 0310-0100 5.46 9.0 µM 2.69E-8 RPP1 & pyrimidine depletion 5 2.41E-8
2 SGTC_368 fentichlor 4.71 4.6 µM 1.33E-6 14 1.24E-6
3 SGTC_1112 tnp00069 4.56 1.2 µM 2.73E-6 13 2.58E-6
4 SGTC_1115 galangin 4.40 695.7 nM 5.81E-6 RPP1 & pyrimidine depletion 5 5.53E-6
5 SGTC_2593 nobiletin 4.40 86.2 µM 5.81E-6 19 5.53E-6
6 SGTC_534 k292-0788 4.39 63.4 µM 6.03E-6 mitochondrial processes 22 5.74E-6
7 SGTC_1033 k072-0102 4.31 21.3 µM 8.49E-6 60S ribosome export 13 8.11E-6
8 SGTC_2284 7945075 4.30 39.2 µM 9.08E-6 mitochondrial processes 19 8.69E-6
9 SGTC_2066 5222451 4.20 176.0 µM 1.38E-5 heme biosynthesis & mitochondrial translocase 27 1.32E-5
10 SGTC_1650 st011916 4.07 45.8 µM 2.41E-5 12 2.33E-5
11 SGTC_2671 danthron 4.04 100.0 µM 2.75E-5 DNA damage response 38 2.66E-5
12 SGTC_2841 9004761 3.86 39.0 µM 5.88E-5 amide catabolism 31 5.73E-5
13 SGTC_211 3455-0679 3.83 143.5 µM 6.50E-5 24 6.35E-5
14 SGTC_875 0709-0210 3.80 25.7 µM 7.42E-5 fatty acid desaturase (OLE1) 34 7.25E-5
15 SGTC_551 k048-0028 3.71 34.9 µM 1.05E-4 22 1.03E-4
16 SGTC_1445 4239-0156 3.69 97.7 µM 1.16E-4 36 1.14E-4
17 SGTC_538 2922-0838 3.65 53.3 µM 1.33E-4 Golgi 40 1.30E-4
18 SGTC_2322 7413319 3.60 38.2 µM 1.59E-4 ERAD & cell cycle 179 1.56E-4
19 SGTC_2443 5493264 3.55 126.7 µM 1.94E-4 33 1.92E-4
20 SGTC_2290 7904846 3.53 200.0 µM 2.13E-4 40 2.10E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.094 4.57E-8 YJL172W CPS1 hom Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions
0.087 4.18E-7 YKL082C RRP14 het Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family
0.086 5.74E-7 YOR017W PET127 hom Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane
0.085 9.32E-7 YLR003C CMS1 hom Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.083 1.37E-6 YJL148W RPA34 hom RNA polymerase I subunit A34.5
0.081 2.47E-6 YMR195W ICY1 hom Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation
0.079 5.29E-6 YGR204C-A_p YGR204C-A_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.078 5.45E-6 YLR401C DUS3 hom Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress
0.076 1.16E-5 YMR196W_p YMR196W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene
0.073 2.48E-5 YNR028W CPR8 hom Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway
0.073 2.59E-5 YNL006W LST8 het Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat
0.071 3.43E-5 YBL077W_d YBL077W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C
0.069 6.73E-5 YPR176C BET2 het Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
0.069 6.74E-5 YJL042W MHP1 hom Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins
0.068 8.21E-5 YLR126C YLR126C hom Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress