YMR179W / SPT21

Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR179W / SPT21

Click on Significant Values for Screen Details ID:SGTC_1|Compound:0258-0098|FD-Score:-4.13|P-value:2.65E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:4.30|P-value:1.62E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:4.55|P-value:5.55E-6 ID:SGTC_179|Compound:1273-0063|FD-Score:3.41|P-value:4.96E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:3.66|P-value:2.07E-4 ID:SGTC_251|Compound:melphalan|FD-Score:5.87|P-value:6.60E-9 ID:SGTC_394|Compound:menadione|FD-Score:3.51|P-value:3.53E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:4.60|P-value:4.28E-6 ID:SGTC_418|Compound:lividomycin a|FD-Score:5.20|P-value:2.42E-7 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.42|P-value:4.84E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:3.55|P-value:3.05E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:5.33|P-value:1.22E-7 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.39|P-value:4.45E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:3.53|P-value:3.25E-4 ID:SGTC_584|Compound:0986-0246|FD-Score:3.80|P-value:1.21E-4 ID:SGTC_593|Compound:1203-0218|FD-Score:3.57|P-value:2.81E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:3.21|P-value:9.84E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:4.01|P-value:5.38E-5 ID:SGTC_733|Compound:4069-0007|FD-Score:3.71|P-value:1.70E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.49|P-value:3.78E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:3.24|P-value:8.85E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:3.23|P-value:9.11E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:3.54|P-value:3.14E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.17|P-value:2.81E-5 ID:SGTC_1213|Compound:1315-0166|FD-Score:3.21|P-value:9.90E-4 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:4.52|P-value:6.20E-6 ID:SGTC_1693|Compound:xanthone|FD-Score:3.47|P-value:4.09E-4 ID:SGTC_1803|Compound:st050068|FD-Score:-3.28|P-value:6.53E-4 ID:SGTC_1810|Compound:st050138|FD-Score:-3.23|P-value:7.68E-4 ID:SGTC_1811|Compound:st050069|FD-Score:-4.79|P-value:1.44E-6 ID:SGTC_1817|Compound:st052116|FD-Score:-3.75|P-value:1.19E-4 ID:SGTC_1843|Compound:st055809|FD-Score:-4.46|P-value:6.31E-6 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.89|P-value:8.60E-5 ID:SGTC_1915|Compound:st067105|FD-Score:3.75|P-value:1.49E-4 ID:SGTC_1932|Compound:st074806|FD-Score:3.23|P-value:9.20E-4 ID:SGTC_1993|Compound:st073995|FD-Score:3.32|P-value:6.78E-4 ID:SGTC_2254|Compound:7938975|FD-Score:-4.08|P-value:3.23E-5 ID:SGTC_2292|Compound:7671323|FD-Score:3.84|P-value:1.04E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-3.39|P-value:4.54E-4 ID:SGTC_2342|Compound:7990637|FD-Score:4.71|P-value:2.58E-6 ID:SGTC_2434|Compound:5670830|FD-Score:3.57|P-value:2.89E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:5.67|P-value:2.08E-8 ID:SGTC_2756|Compound:carbadox|FD-Score:3.81|P-value:1.17E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.77|P-value:1.36E-4 ID:SGTC_3055|Compound:9094636|FD-Score:-3.65|P-value:1.73E-4 ID:SGTC_3136|Compound:9095672|FD-Score:3.30|P-value:7.25E-4 ID:SGTC_3171|Compound:9103192|FD-Score:4.17|P-value:2.76E-5 ID:SGTC_3249|Compound:9135183|FD-Score:3.93|P-value:7.21E-5 ID:SGTC_3356|Compound:9151811|FD-Score:3.35|P-value:6.08E-4 ID:SGTC_1|Compound:0258-0098|FD-Score:-4.13|P-value:2.65E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:4.30|P-value:1.62E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:4.55|P-value:5.55E-6 ID:SGTC_179|Compound:1273-0063|FD-Score:3.41|P-value:4.96E-4 ID:SGTC_198|Compound:3454-2873|FD-Score:3.66|P-value:2.07E-4 ID:SGTC_251|Compound:melphalan|FD-Score:5.87|P-value:6.60E-9 ID:SGTC_394|Compound:menadione|FD-Score:3.51|P-value:3.53E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:4.60|P-value:4.28E-6 ID:SGTC_418|Compound:lividomycin a|FD-Score:5.20|P-value:2.42E-7 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.42|P-value:4.84E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:3.55|P-value:3.05E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:5.33|P-value:1.22E-7 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.39|P-value:4.45E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:3.53|P-value:3.25E-4 ID:SGTC_584|Compound:0986-0246|FD-Score:3.80|P-value:1.21E-4 ID:SGTC_593|Compound:1203-0218|FD-Score:3.57|P-value:2.81E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:3.21|P-value:9.84E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:4.01|P-value:5.38E-5 ID:SGTC_733|Compound:4069-0007|FD-Score:3.71|P-value:1.70E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.49|P-value:3.78E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:3.24|P-value:8.85E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:3.23|P-value:9.11E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:3.54|P-value:3.14E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.17|P-value:2.81E-5 ID:SGTC_1213|Compound:1315-0166|FD-Score:3.21|P-value:9.90E-4 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:4.52|P-value:6.20E-6 ID:SGTC_1693|Compound:xanthone|FD-Score:3.47|P-value:4.09E-4 ID:SGTC_1803|Compound:st050068|FD-Score:-3.28|P-value:6.53E-4 ID:SGTC_1810|Compound:st050138|FD-Score:-3.23|P-value:7.68E-4 ID:SGTC_1811|Compound:st050069|FD-Score:-4.79|P-value:1.44E-6 ID:SGTC_1817|Compound:st052116|FD-Score:-3.75|P-value:1.19E-4 ID:SGTC_1843|Compound:st055809|FD-Score:-4.46|P-value:6.31E-6 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.89|P-value:8.60E-5 ID:SGTC_1915|Compound:st067105|FD-Score:3.75|P-value:1.49E-4 ID:SGTC_1932|Compound:st074806|FD-Score:3.23|P-value:9.20E-4 ID:SGTC_1993|Compound:st073995|FD-Score:3.32|P-value:6.78E-4 ID:SGTC_2254|Compound:7938975|FD-Score:-4.08|P-value:3.23E-5 ID:SGTC_2292|Compound:7671323|FD-Score:3.84|P-value:1.04E-4 ID:SGTC_2322|Compound:7413319|FD-Score:-3.39|P-value:4.54E-4 ID:SGTC_2342|Compound:7990637|FD-Score:4.71|P-value:2.58E-6 ID:SGTC_2434|Compound:5670830|FD-Score:3.57|P-value:2.89E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:5.67|P-value:2.08E-8 ID:SGTC_2756|Compound:carbadox|FD-Score:3.81|P-value:1.17E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.77|P-value:1.36E-4 ID:SGTC_3055|Compound:9094636|FD-Score:-3.65|P-value:1.73E-4 ID:SGTC_3136|Compound:9095672|FD-Score:3.30|P-value:7.25E-4 ID:SGTC_3171|Compound:9103192|FD-Score:4.17|P-value:2.76E-5 ID:SGTC_3249|Compound:9135183|FD-Score:3.93|P-value:7.21E-5 ID:SGTC_3356|Compound:9151811|FD-Score:3.35|P-value:6.08E-4

Top fitness defect scores for YMR179W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_251 melphalan 5.87 456.5 µM 6.60E-9 DNA damage response 7 2.17E-9
2 SGTC_2577 2',4'-dihydroxy-4-methoxychalcone 5.67 15.3 µM 2.08E-8 8 7.30E-9
3 SGTC_514 4466-0038 5.33 2.6 nM 1.22E-7 cell wall 95 4.79E-8
4 SGTC_418 lividomycin a 5.20 100.0 µM 2.42E-7 3 9.88E-8
5 SGTC_2342 7990637 4.71 118.9 µM 2.58E-6 RNA processing & uracil transport 19 1.22E-6
6 SGTC_404 fumagillin 4.60 50.0 µM 4.28E-6 10 2.08E-6
7 SGTC_168 3970-0862 4.55 530.5 µM 5.55E-6 88 2.74E-6
8 SGTC_1539 benzyladenosine 4.52 56.0 µM 6.20E-6 6 3.09E-6
9 SGTC_115 0527-0192 4.30 112.0 µM 1.62E-5 56 8.53E-6
10 SGTC_3171 9103192 4.17 49.5 µM 2.76E-5 5 1.50E-5
11 SGTC_1133 3473-0247 4.17 50.0 µM 2.81E-5 RSC complex & mRNA processing 39 1.53E-5
12 SGTC_673 1082-0474 4.01 110.0 µM 5.38E-5 RSC complex & mRNA processing 63 3.05E-5
13 SGTC_3249 9135183 3.93 49.5 µM 7.21E-5 60S ribosome export 9 4.16E-5
14 SGTC_1844 rapamycin 3.89 4.0 nM 8.60E-5 164 5.02E-5
15 SGTC_2292 7671323 3.84 168.1 µM 1.04E-4 RNA processing & uracil transport 36 6.14E-5
16 SGTC_2756 carbadox 3.81 75.1 µM 1.17E-4 63 6.96E-5
17 SGTC_584 0986-0246 3.80 15.4 µM 1.21E-4 heme requiring 15 7.22E-5
18 SGTC_2857 9024691 3.77 58.4 µM 1.36E-4 27 8.15E-5
19 SGTC_1915 st067105 3.75 30.6 µM 1.49E-4 RNA pol III & RNase P/MRP 52 8.99E-5
20 SGTC_733 4069-0007 3.71 56.1 µM 1.70E-4 ERAD & cell cycle 94 1.04E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.155 2.01E-19 YDR121W DPB4 hom Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
0.131 2.75E-14 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.122 1.56E-12 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.119 4.05E-12 YMR036C MIH1 hom Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.116 1.71E-11 YBR020W GAL1 hom Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
0.114 4.38E-11 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.112 6.94E-11 YBR091C TIM12 het Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
0.111 1.10E-10 YOR091W TMA46 hom Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
0.110 1.46E-10 YPR061C JID1 hom Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
0.106 7.98E-10 YDR049W VMS1 hom Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
0.099 8.69E-9 YML114C TAF8 het TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation
0.099 8.92E-9 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.096 2.94E-8 YGR211W ZPR1 het Essential protein with two zinc fingers; present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p); relative distribution to the nucleus increases upon DNA replication stress
0.096 2.93E-8 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.094 5.06E-8 YBL061C SKT5 hom Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication