YMR191W / SPG5

Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR191W / SPG5

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:5.43|P-value:7.08E-8 ID:SGTC_323|Compound:4013-0906|FD-Score:3.52|P-value:3.28E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:3.95|P-value:6.64E-5 ID:SGTC_422|Compound:cantharidin|FD-Score:3.23|P-value:8.75E-4 ID:SGTC_814|Compound:3342-0569|FD-Score:4.70|P-value:2.63E-6 ID:SGTC_872|Compound:0588-0139|FD-Score:3.52|P-value:3.30E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:3.24|P-value:8.56E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:-3.30|P-value:6.56E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.44|P-value:4.40E-4 ID:SGTC_917|Compound:harmaline|FD-Score:3.71|P-value:1.65E-4 ID:SGTC_1323|Compound:1348-1351|FD-Score:4.02|P-value:4.87E-5 ID:SGTC_1504|Compound:k297-0033|FD-Score:-3.21|P-value:8.78E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:3.37|P-value:5.58E-4 ID:SGTC_1674|Compound:st016616|FD-Score:4.07|P-value:4.11E-5 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-3.22|P-value:8.57E-4 ID:SGTC_1856|Compound:st057054|FD-Score:3.52|P-value:3.25E-4 ID:SGTC_1949|Compound:st076024|FD-Score:4.29|P-value:1.66E-5 ID:SGTC_2072|Compound:5231554|FD-Score:3.43|P-value:4.52E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.31|P-value:6.68E-4 ID:SGTC_2367|Compound:9070478|FD-Score:3.66|P-value:1.99E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.65|P-value:2.08E-4 ID:SGTC_2516|Compound:gossypol|FD-Score:3.30|P-value:7.08E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:4.17|P-value:2.65E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:-3.19|P-value:9.50E-4 ID:SGTC_2804|Compound:7954240|FD-Score:3.77|P-value:1.29E-4 ID:SGTC_2812|Compound:7970281|FD-Score:3.48|P-value:3.71E-4 ID:SGTC_2994|Compound:9053520|FD-Score:-4.29|P-value:1.51E-5 ID:SGTC_3023|Compound:9089636|FD-Score:3.39|P-value:5.11E-4 ID:SGTC_3030|Compound:9087619|FD-Score:-3.50|P-value:3.32E-4 ID:SGTC_3211|Compound:9128781|FD-Score:3.87|P-value:8.83E-5 ID:SGTC_3325|Compound:9138760|FD-Score:3.83|P-value:1.03E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:5.43|P-value:7.08E-8 ID:SGTC_323|Compound:4013-0906|FD-Score:3.52|P-value:3.28E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:3.95|P-value:6.64E-5 ID:SGTC_422|Compound:cantharidin|FD-Score:3.23|P-value:8.75E-4 ID:SGTC_814|Compound:3342-0569|FD-Score:4.70|P-value:2.63E-6 ID:SGTC_872|Compound:0588-0139|FD-Score:3.52|P-value:3.30E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:3.24|P-value:8.56E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:-3.30|P-value:6.56E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.44|P-value:4.40E-4 ID:SGTC_917|Compound:harmaline|FD-Score:3.71|P-value:1.65E-4 ID:SGTC_1323|Compound:1348-1351|FD-Score:4.02|P-value:4.87E-5 ID:SGTC_1504|Compound:k297-0033|FD-Score:-3.21|P-value:8.78E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:3.37|P-value:5.58E-4 ID:SGTC_1674|Compound:st016616|FD-Score:4.07|P-value:4.11E-5 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-3.22|P-value:8.57E-4 ID:SGTC_1856|Compound:st057054|FD-Score:3.52|P-value:3.25E-4 ID:SGTC_1949|Compound:st076024|FD-Score:4.29|P-value:1.66E-5 ID:SGTC_2072|Compound:5231554|FD-Score:3.43|P-value:4.52E-4 ID:SGTC_2293|Compound:7624732|FD-Score:3.31|P-value:6.68E-4 ID:SGTC_2367|Compound:9070478|FD-Score:3.66|P-value:1.99E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.65|P-value:2.08E-4 ID:SGTC_2516|Compound:gossypol|FD-Score:3.30|P-value:7.08E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:4.17|P-value:2.65E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:-3.19|P-value:9.50E-4 ID:SGTC_2804|Compound:7954240|FD-Score:3.77|P-value:1.29E-4 ID:SGTC_2812|Compound:7970281|FD-Score:3.48|P-value:3.71E-4 ID:SGTC_2994|Compound:9053520|FD-Score:-4.29|P-value:1.51E-5 ID:SGTC_3023|Compound:9089636|FD-Score:3.39|P-value:5.11E-4 ID:SGTC_3030|Compound:9087619|FD-Score:-3.50|P-value:3.32E-4 ID:SGTC_3211|Compound:9128781|FD-Score:3.87|P-value:8.83E-5 ID:SGTC_3325|Compound:9138760|FD-Score:3.83|P-value:1.03E-4

Top fitness defect scores for YMR191W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_6 0844-0013 5.43 19.8 µM 7.08E-8 25 2.80E-8
2 SGTC_814 3342-0569 4.70 96.0 µM 2.63E-6 12 1.30E-6
3 SGTC_1949 st076024 4.29 69.1 µM 1.66E-5 13 9.13E-6
4 SGTC_2571 chrysanthemic acid 4.17 100.0 µM 2.65E-5 10 1.50E-5
5 SGTC_1674 st016616 4.07 23.6 µM 4.11E-5 24 2.39E-5
6 SGTC_1323 1348-1351 4.02 11.2 µM 4.87E-5 6 2.86E-5
7 SGTC_374 0880-0231 3.95 82.3 µM 6.64E-5 iron homeostasis 20 3.97E-5
8 SGTC_3211 9128781 3.87 49.5 µM 8.83E-5 61 5.37E-5
9 SGTC_3325 9138760 3.83 55.2 µM 1.03E-4 Golgi 33 6.34E-5
10 SGTC_2804 7954240 3.77 19.5 µM 1.29E-4 39 8.06E-5
11 SGTC_917 harmaline 3.71 728.1 nM 1.65E-4 ergosterol biosynthesis 32 1.04E-4
12 SGTC_2367 9070478 3.66 135.4 µM 1.99E-4 cell wall 42 1.27E-4
13 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.65 3.7 µM 2.08E-4 107 1.34E-4
14 SGTC_1856 st057054 3.52 55.7 µM 3.25E-4 37 2.14E-4
15 SGTC_323 4013-0906 3.52 34.3 µM 3.28E-4 ERG2 16 2.16E-4
16 SGTC_872 0588-0139 3.52 234.0 µM 3.30E-4 45 2.18E-4
17 SGTC_2812 7970281 3.48 58.4 µM 3.71E-4 52 2.46E-4
18 SGTC_915 methyl methanesulfonate (MMS) 3.44 110.0 mM 4.40E-4 DNA damage response 61 2.95E-4
19 SGTC_2072 5231554 3.43 129.0 µM 4.52E-4 21 3.04E-4
20 SGTC_3023 9089636 3.39 49.5 µM 5.11E-4 8 3.45E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.190 1.17E-28 YOR214C_p YOR214C_p hom Putative protein of unknown function; YOR214C is not an essential gene
0.158 2.85E-20 YDL018C ERP3 hom Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.146 1.48E-17 YHR021W-A_p ECM12_p hom Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.144 6.61E-17 YMR080C NAM7 hom ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress
0.141 1.80E-16 YKR013W PRY2 hom Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication
0.140 3.77E-16 YLR288C MEC3 hom DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
0.140 4.52E-16 YJR046W TAH11 het DNA replication licensing factor, required for pre-replication complex assembly
0.137 2.02E-15 YNL242W ATG2 hom Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.133 8.52E-15 YBR265W TSC10 het 3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family
0.129 6.22E-14 YPL004C LSP1 hom Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family
0.126 2.06E-13 YDL029W ARP2 het Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
0.126 2.25E-13 YOR155C ISN1 hom Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.122 1.15E-12 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.118 6.02E-12 YJL138C TIF2 hom Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication
0.117 9.78E-12 YLR398C SKI2 hom Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay