YMR200W / ROT1

Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YMR200W / ROT1

Click on Significant Values for Screen Details ID:SGTC_257|Compound:daunorubicin|FD-Score:3.56|P-value:4.30E-5 ID:SGTC_377|Compound:3970-0964|FD-Score:2.91|P-value:6.46E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:3.88|P-value:9.52E-6 ID:SGTC_394|Compound:menadione|FD-Score:-4.24|P-value:2.09E-6 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.18|P-value:2.22E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:3.38|P-value:9.75E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:3.22|P-value:1.92E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:3.62|P-value:3.27E-5 ID:SGTC_651|Compound:3253-0966|FD-Score:3.29|P-value:1.46E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:10.60|P-value:1.82E-31 ID:SGTC_708|Compound:3448-5400|FD-Score:4.91|P-value:3.33E-8 ID:SGTC_778|Compound:3456-2434|FD-Score:-4.82|P-value:8.21E-8 ID:SGTC_800|Compound:3448-7362|FD-Score:11.20|P-value:4.21E-35 ID:SGTC_876|Compound:0720-0963|FD-Score:2.94|P-value:5.95E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:2.90|P-value:6.87E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:4.77|P-value:7.70E-8 ID:SGTC_1062|Compound:mesoridazine|FD-Score:-3.25|P-value:2.14E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:2.84|P-value:8.42E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:3.79|P-value:1.50E-5 ID:SGTC_1482|Compound:3346-2049|FD-Score:4.61|P-value:2.02E-7 ID:SGTC_1506|Compound:4226-2149|FD-Score:4.45|P-value:4.77E-7 ID:SGTC_1524|Compound:corynanthine|FD-Score:-2.93|P-value:7.56E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:3.25|P-value:1.72E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:-3.14|P-value:3.29E-4 ID:SGTC_1713|Compound:st033146|FD-Score:-3.62|P-value:4.30E-5 ID:SGTC_1716|Compound:st033144|FD-Score:-5.95|P-value:4.67E-11 ID:SGTC_1721|Compound:st082152|FD-Score:-4.86|P-value:6.51E-8 ID:SGTC_1731|Compound:st035964|FD-Score:3.70|P-value:2.30E-5 ID:SGTC_1762|Compound:st045503|FD-Score:2.92|P-value:6.32E-4 ID:SGTC_1784|Compound:st039334|FD-Score:-2.94|P-value:7.39E-4 ID:SGTC_1838|Compound:st055369|FD-Score:-3.25|P-value:2.18E-4 ID:SGTC_1842|Compound:st055362|FD-Score:-3.19|P-value:2.75E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:5.52|P-value:6.49E-10 ID:SGTC_1979|Compound:st071809|FD-Score:3.73|P-value:1.98E-5 ID:SGTC_1982|Compound:st072467|FD-Score:6.70|P-value:9.13E-14 ID:SGTC_1986|Compound:st071812|FD-Score:2.82|P-value:9.16E-4 ID:SGTC_1996|Compound:st072960|FD-Score:3.34|P-value:1.15E-4 ID:SGTC_2007|Compound:4011539|FD-Score:4.77|P-value:7.68E-8 ID:SGTC_2009|Compound:4012297|FD-Score:5.22|P-value:4.85E-9 ID:SGTC_2125|Compound:5246583|FD-Score:3.18|P-value:2.30E-4 ID:SGTC_2240|Compound:6690706|FD-Score:-3.19|P-value:2.73E-4 ID:SGTC_2289|Compound:7946404|FD-Score:5.01|P-value:1.86E-8 ID:SGTC_2330|Compound:7979373|FD-Score:3.14|P-value:2.69E-4 ID:SGTC_2345|Compound:9052315|FD-Score:-3.17|P-value:2.95E-4 ID:SGTC_2399|Compound:5884133|FD-Score:5.73|P-value:1.52E-10 ID:SGTC_2424|Compound:5537928|FD-Score:2.80|P-value:9.94E-4 ID:SGTC_2448|Compound:5185112|FD-Score:-3.56|P-value:5.60E-5 ID:SGTC_2452|Compound:5863136|FD-Score:-3.43|P-value:1.00E-4 ID:SGTC_2453|Compound:5731132|FD-Score:5.80|P-value:9.58E-11 ID:SGTC_2460|Compound:5552655|FD-Score:2.83|P-value:8.83E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.30|P-value:1.37E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.76|P-value:1.76E-5 ID:SGTC_2602|Compound:kawain|FD-Score:4.20|P-value:1.92E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:-2.95|P-value:7.14E-4 ID:SGTC_2910|Compound:7948405|FD-Score:3.94|P-value:7.21E-6 ID:SGTC_257|Compound:daunorubicin|FD-Score:3.56|P-value:4.30E-5 ID:SGTC_377|Compound:3970-0964|FD-Score:2.91|P-value:6.46E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:3.88|P-value:9.52E-6 ID:SGTC_394|Compound:menadione|FD-Score:-4.24|P-value:2.09E-6 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.18|P-value:2.22E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:3.38|P-value:9.75E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:3.22|P-value:1.92E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:3.62|P-value:3.27E-5 ID:SGTC_651|Compound:3253-0966|FD-Score:3.29|P-value:1.46E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:10.60|P-value:1.82E-31 ID:SGTC_708|Compound:3448-5400|FD-Score:4.91|P-value:3.33E-8 ID:SGTC_778|Compound:3456-2434|FD-Score:-4.82|P-value:8.21E-8 ID:SGTC_800|Compound:3448-7362|FD-Score:11.20|P-value:4.21E-35 ID:SGTC_876|Compound:0720-0963|FD-Score:2.94|P-value:5.95E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:2.90|P-value:6.87E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:4.77|P-value:7.70E-8 ID:SGTC_1062|Compound:mesoridazine|FD-Score:-3.25|P-value:2.14E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:2.84|P-value:8.42E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:3.79|P-value:1.50E-5 ID:SGTC_1482|Compound:3346-2049|FD-Score:4.61|P-value:2.02E-7 ID:SGTC_1506|Compound:4226-2149|FD-Score:4.45|P-value:4.77E-7 ID:SGTC_1524|Compound:corynanthine|FD-Score:-2.93|P-value:7.56E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:3.25|P-value:1.72E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:-3.14|P-value:3.29E-4 ID:SGTC_1713|Compound:st033146|FD-Score:-3.62|P-value:4.30E-5 ID:SGTC_1716|Compound:st033144|FD-Score:-5.95|P-value:4.67E-11 ID:SGTC_1721|Compound:st082152|FD-Score:-4.86|P-value:6.51E-8 ID:SGTC_1731|Compound:st035964|FD-Score:3.70|P-value:2.30E-5 ID:SGTC_1762|Compound:st045503|FD-Score:2.92|P-value:6.32E-4 ID:SGTC_1784|Compound:st039334|FD-Score:-2.94|P-value:7.39E-4 ID:SGTC_1838|Compound:st055369|FD-Score:-3.25|P-value:2.18E-4 ID:SGTC_1842|Compound:st055362|FD-Score:-3.19|P-value:2.75E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:5.52|P-value:6.49E-10 ID:SGTC_1979|Compound:st071809|FD-Score:3.73|P-value:1.98E-5 ID:SGTC_1982|Compound:st072467|FD-Score:6.70|P-value:9.13E-14 ID:SGTC_1986|Compound:st071812|FD-Score:2.82|P-value:9.16E-4 ID:SGTC_1996|Compound:st072960|FD-Score:3.34|P-value:1.15E-4 ID:SGTC_2007|Compound:4011539|FD-Score:4.77|P-value:7.68E-8 ID:SGTC_2009|Compound:4012297|FD-Score:5.22|P-value:4.85E-9 ID:SGTC_2125|Compound:5246583|FD-Score:3.18|P-value:2.30E-4 ID:SGTC_2240|Compound:6690706|FD-Score:-3.19|P-value:2.73E-4 ID:SGTC_2289|Compound:7946404|FD-Score:5.01|P-value:1.86E-8 ID:SGTC_2330|Compound:7979373|FD-Score:3.14|P-value:2.69E-4 ID:SGTC_2345|Compound:9052315|FD-Score:-3.17|P-value:2.95E-4 ID:SGTC_2399|Compound:5884133|FD-Score:5.73|P-value:1.52E-10 ID:SGTC_2424|Compound:5537928|FD-Score:2.80|P-value:9.94E-4 ID:SGTC_2448|Compound:5185112|FD-Score:-3.56|P-value:5.60E-5 ID:SGTC_2452|Compound:5863136|FD-Score:-3.43|P-value:1.00E-4 ID:SGTC_2453|Compound:5731132|FD-Score:5.80|P-value:9.58E-11 ID:SGTC_2460|Compound:5552655|FD-Score:2.83|P-value:8.83E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.30|P-value:1.37E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.76|P-value:1.76E-5 ID:SGTC_2602|Compound:kawain|FD-Score:4.20|P-value:1.92E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:-2.95|P-value:7.14E-4 ID:SGTC_2910|Compound:7948405|FD-Score:3.94|P-value:7.21E-6

Top fitness defect scores for YMR200W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_800 3448-7362 11.20 155.0 µM 4.21E-35 translation 4 1.48E-29 2.06
2 SGTC_663 1486-0733 10.60 13.9 µM 1.82E-31 cell wall signaling 2 1.61E-26 2.93
3 SGTC_1982 st072467 6.70 71.9 µM 9.13E-14 1 1.01E-11 1.33
4 SGTC_2453 5731132 5.80 22.2 µM 9.58E-11 1 3.38E-9 0.87
5 SGTC_2399 5884133 5.73 200.0 µM 1.52E-10 cell wall signaling 8 4.99E-9 0.27
6 SGTC_1844 rapamycin 5.52 4.0 nM 6.49E-10 27 1.68E-8 0.12
7 SGTC_2009 4012297 5.22 12.1 µM 4.85E-9 cell wall signaling 5 9.03E-8 0.42
8 SGTC_2289 7946404 5.01 165.1 µM 1.86E-8 11 2.78E-7 6.93E-4
9 SGTC_708 3448-5400 4.91 146.0 µM 3.33E-8 8 4.53E-7 0.09
10 SGTC_2007 4011539 4.77 71.8 µM 7.68E-8 cell wall signaling 5 9.11E-7 0.41
11 SGTC_933 2914-0560 4.77 114.0 µM 7.70E-8 3 9.13E-7 0.57
12 SGTC_1482 3346-2049 4.61 8.5 µM 2.02E-7 4 2.05E-6 0.26
13 SGTC_1506 4226-2149 4.45 212.0 µM 4.77E-7 2 4.20E-6 0.30
14 SGTC_2602 kawain 4.20 100.0 µM 1.92E-6 5 1.35E-5 0.60
15 SGTC_2910 7948405 3.94 26.0 µM 7.21E-6 plasma membrane duress 4 4.09E-5 0.43
16 SGTC_390 dec-rvkr-cmk 3.88 16.7 µM 9.52E-6 calcium & mitochondrial duress 6 5.16E-5 0.08
17 SGTC_1434 4073-0095 3.79 163.0 µM 1.50E-5 3 7.54E-5 0.33
18 SGTC_2595 hexamethylquercetagetin 3.76 100.0 µM 1.76E-5 7 8.66E-5 0.01
19 SGTC_1979 st071809 3.73 45.3 µM 1.98E-5 RPP1 & pyrimidine depletion 9 9.54E-5 0.40
20 SGTC_1731 st035964 3.70 63.0 µM 2.30E-5 16 1.08E-4 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.330 5.87E-86 YNL322C KRE1 hom Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.318 8.45E-80 YAL056C-A_d YAL056C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.316 1.12E-78 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.305 4.80E-73 YAL058W CNE1 hom Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
0.290 6.41E-66 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.283 7.89E-63 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.275 3.03E-59 YAL053W FLC2 hom Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
0.273 2.36E-58 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.228 5.72E-41 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.222 8.27E-39 YKL096C-B_p YKL096C-B_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.219 9.82E-38 YNL084C END3 hom EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
0.212 2.63E-35 YBR229C ROT2 hom Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
0.206 2.05E-33 YJL139C YUR1 hom Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication
0.199 3.62E-31 YJL183W MNN11 hom Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
0.194 1.07E-29 YGL250W RMR1 hom Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus