HIPHOP chemogenomics database

YMR200W / ROT1

Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation

Zygosity: Heterozygous strain

fixedexpanded
Profile for YMR200W / ROT1

Click on Significant Values for Screen Details

Top fitness defect scores for YMR200W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_800	3448-7362	11.20	155.0 µM	4.21E-35	translation	4	1.48E-29	2.06
2	SGTC_663	1486-0733	10.60	13.9 µM	1.82E-31	cell wall signaling	2	1.61E-26	2.93
3	SGTC_1982	st072467	6.70	71.9 µM	9.13E-14		1	1.01E-11	1.33
4	SGTC_2453	5731132	5.80	22.2 µM	9.58E-11		1	3.38E-9	0.87
5	SGTC_2399	5884133	5.73	200.0 µM	1.52E-10	cell wall signaling	8	4.99E-9	0.27
6	SGTC_1844	rapamycin	5.52	4.0 nM	6.49E-10		27	1.68E-8	0.12
7	SGTC_2009	4012297	5.22	12.1 µM	4.85E-9	cell wall signaling	5	9.03E-8	0.42
8	SGTC_2289	7946404	5.01	165.1 µM	1.86E-8		11	2.78E-7	6.93E-4
9	SGTC_708	3448-5400	4.91	146.0 µM	3.33E-8		8	4.53E-7	0.09
10	SGTC_2007	4011539	4.77	71.8 µM	7.68E-8	cell wall signaling	5	9.11E-7	0.41
11	SGTC_933	2914-0560	4.77	114.0 µM	7.70E-8		3	9.13E-7	0.57
12	SGTC_1482	3346-2049	4.61	8.5 µM	2.02E-7		4	2.05E-6	0.26
13	SGTC_1506	4226-2149	4.45	212.0 µM	4.77E-7		2	4.20E-6	0.30
14	SGTC_2602	kawain	4.20	100.0 µM	1.92E-6		5	1.35E-5	0.60
15	SGTC_2910	7948405	3.94	26.0 µM	7.21E-6	plasma membrane duress	4	4.09E-5	0.43
16	SGTC_390	dec-rvkr-cmk	3.88	16.7 µM	9.52E-6	calcium & mitochondrial duress	6	5.16E-5	0.08
17	SGTC_1434	4073-0095	3.79	163.0 µM	1.50E-5		3	7.54E-5	0.33
18	SGTC_2595	hexamethylquercetagetin	3.76	100.0 µM	1.76E-5		7	8.66E-5	0.01
19	SGTC_1979	st071809	3.73	45.3 µM	1.98E-5	RPP1 & pyrimidine depletion	9	9.54E-5	0.40
20	SGTC_1731	st035964	3.70	63.0 µM	2.30E-5		16	1.08E-4	0.02

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.330	5.87E-86	YNL322C	KRE1	hom	Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.318	8.45E-80	YAL056C-A_d	YAL056C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.316	1.12E-78	YOR085W	OST3	hom	Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.305	4.80E-73	YAL058W	CNE1	hom	Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
0.290	6.41E-66	YBR260C	RGD1	hom	GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.283	7.89E-63	YDL096C_d	OPI6_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.275	3.03E-59	YAL053W	FLC2	hom	Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
0.273	2.36E-58	YGR196C	FYV8	hom	Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.228	5.72E-41	YDL203C	ACK1	hom	Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.222	8.27E-39	YKL096C-B_p	YKL096C-B_p	hom	Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.219	9.82E-38	YNL084C	END3	hom	EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
0.212	2.63E-35	YBR229C	ROT2	hom	Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
0.206	2.05E-33	YJL139C	YUR1	hom	Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication
0.199	3.62E-31	YJL183W	MNN11	hom	Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
0.194	1.07E-29	YGL250W	RMR1	hom	Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus