Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
Zygosity: Heterozygous strain
fixedexpanded
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Top fitness defect scores for YMR200W deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.330 | 5.87E-86 | YNL322C | KRE1 | hom | Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor |
0.318 | 8.45E-80 | YAL056C-A_d | YAL056C-A_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data |
0.316 | 1.12E-78 | YOR085W | OST3 | hom | Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins |
0.305 | 4.80E-73 | YAL058W | CNE1 | hom | Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast |
0.290 | 6.41E-66 | YBR260C | RGD1 | hom | GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization |
0.283 | 7.89E-63 | YDL096C_d | OPI6_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential |
0.275 | 3.03E-59 | YAL053W | FLC2 | hom | Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication |
0.273 | 2.36E-58 | YGR196C | FYV8 | hom | Protein of unknown function, required for survival upon exposure to K1 killer toxin |
0.228 | 5.72E-41 | YDL203C | ACK1 | hom | Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria |
0.222 | 8.27E-39 | YKL096C-B_p | YKL096C-B_p | hom | Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching |
0.219 | 9.82E-38 | YNL084C | END3 | hom | EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p |
0.212 | 2.63E-35 | YBR229C | ROT2 | hom | Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations |
0.206 | 2.05E-33 | YJL139C | YUR1 | hom | Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication |
0.199 | 3.62E-31 | YJL183W | MNN11 | hom | Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p |
0.194 | 1.07E-29 | YGL250W | RMR1 | hom | Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus |