C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
Zygosity: Homozygous strain
fixedexpanded
![]() ![]() Click on Significant Values for Screen Details |
Top fitness defect scores for YMR202W deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.383 | 2.75E-117 | YKL212W | SAC1 | hom | Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism |
0.360 | 3.65E-103 | YML007C-A_p | YML007C-A_p | hom | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria |
0.347 | 3.73E-95 | YNL323W | LEM3 | hom | Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane |
0.312 | 2.11E-76 | YIL154C | IMP2' | hom | Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat |
0.304 | 1.19E-72 | YKL126W | YPK1 | hom | Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication |
0.294 | 1.38E-67 | YGL054C | ERV14 | hom | COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication |
0.257 | 9.42E-52 | YGR285C | ZUO1 | hom | Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p |
0.256 | 3.15E-51 | YGL012W | ERG4 | hom | C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol |
0.242 | 6.78E-46 | YLR087C | CSF1 | hom | Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
0.238 | 2.05E-44 | YMR015C | ERG5 | hom | C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs |
0.220 | 4.26E-38 | YBL079W | NUP170 | hom | Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication |
0.210 | 1.57E-34 | YOR039W | CKB2 | hom | Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase |
0.201 | 7.28E-32 | YJL204C | RCY1 | hom | F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth |
0.199 | 3.86E-31 | YIL048W | NEO1 | het | Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus |
0.198 | 8.01E-31 | YDR159W | SAC3 | hom | Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) |