HIPHOP chemogenomics database

YMR202W / ERG2

C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis

Zygosity: Homozygous strain

fixedexpanded
Profile for YMR202W / ERG2

Click on Significant Values for Screen Details

Top fitness defect scores for YMR202W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2322	7413319	34.90	38.2 µM	2.76E-109	ERAD & cell cycle	1	6.24E-267
2	SGTC_1244	0416-0015	34.80	52.5 µM	6.78E-109	ERG2	1	5.71E-266
3	SGTC_2144	5628481	32.40	200.0 µM	1.86E-94	ERG2	1	1.99E-230
4	SGTC_54	0159-0055	29.80	9.0 µM	2.39E-80	ERG2	1	1.08E-195
5	SGTC_1206	3978-0017	28.90	132.0 µM	2.33E-75	ERG2	1	2.07E-183
6	SGTC_1247	0448-0043	28.40	5.8 µM	4.27E-73	ERG2	1	7.76E-178
7	SGTC_2658	polymyxin b sulfate	28.40	77.0 µM	5.51E-73	NEO1	1	1.45E-177
8	SGTC_1826	st055655	27.90	41.6 µM	1.74E-70	ERG2	1	2.06E-171
9	SGTC_234	nsc-17383	27.80	10.7 µM	2.82E-70	ERG2	1	6.77E-171
10	SGTC_2055	5235184	27.60	200.0 µM	5.69E-69	ERG2	1	1.11E-167
11	SGTC_1750	st081859	27.00	2.4 µM	4.07E-66	endomembrane recycling	6	1.18E-160
12	SGTC_1360	1598-0022	26.00	39.8 µM	1.36E-61	ERG2	1	1.64E-149
13	SGTC_2699	st077704	23.90	54.6 µM	3.19E-52	endomembrane recycling	1	1.93E-126
14	SGTC_2300	7976697	23.10	71.6 µM	3.97E-49	ERG2	1	8.09E-119
15	SGTC_2858	9025781	22.80	9.0 µM	8.29E-48	sphingolipid biosynthesis & PDR1	3	1.44E-115
16	SGTC_724	3910-0327	22.50	137.0 µM	1.92E-46	ERG2	1	3.29E-112
17	SGTC_841	0251-0215	21.80	91.2 µM	9.20E-44		1	1.32E-105
18	SGTC_2862	9027634	21.70	71.4 µM	2.00E-43	ERG2	1	8.96E-105
19	SGTC_2523	spermine	21.00	47.7 µM	1.07E-40	calcium & mitochondrial duress	2	4.63E-98
20	SGTC_221	trifluoperazine	20.60	10.3 µM	2.50E-39	NEO1-PIK1	11	1.10E-94

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.383	2.75E-117	YKL212W	SAC1	hom	Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
0.360	3.65E-103	YML007C-A_p	YML007C-A_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria
0.347	3.73E-95	YNL323W	LEM3	hom	Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane
0.312	2.11E-76	YIL154C	IMP2'	hom	Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
0.304	1.19E-72	YKL126W	YPK1	hom	Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
0.294	1.38E-67	YGL054C	ERV14	hom	COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
0.257	9.42E-52	YGR285C	ZUO1	hom	Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
0.256	3.15E-51	YGL012W	ERG4	hom	C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
0.242	6.78E-46	YLR087C	CSF1	hom	Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.238	2.05E-44	YMR015C	ERG5	hom	C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
0.220	4.26E-38	YBL079W	NUP170	hom	Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
0.210	1.57E-34	YOR039W	CKB2	hom	Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
0.201	7.28E-32	YJL204C	RCY1	hom	F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
0.199	3.86E-31	YIL048W	NEO1	het	Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
0.198	8.01E-31	YDR159W	SAC3	hom	Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP)