YMR218C / TRS130

One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy

Zygosity: Heterozygous strain
fixedexpanded
Profile for YMR218C / TRS130

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:6.31|P-value:6.54E-12 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.43|P-value:1.16E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-4.83|P-value:1.05E-7 ID:SGTC_178|Compound:4092-0806|FD-Score:3.73|P-value:3.17E-5 ID:SGTC_207|Compound:4185-0010|FD-Score:3.49|P-value:8.96E-5 ID:SGTC_252|Compound:miconazole|FD-Score:3.37|P-value:1.48E-4 ID:SGTC_278|Compound:4092-0821|FD-Score:2.94|P-value:8.13E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:3.63|P-value:4.99E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:9.53|P-value:7.30E-25 ID:SGTC_320|Compound:4092-0846|FD-Score:5.51|P-value:1.65E-9 ID:SGTC_372|Compound:0155-0182|FD-Score:-5.00|P-value:3.87E-8 ID:SGTC_383|Compound:2324-0395|FD-Score:-3.98|P-value:9.40E-6 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:3.36|P-value:1.58E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-6.86|P-value:8.90E-14 ID:SGTC_519|Compound:k048-0011|FD-Score:3.21|P-value:2.84E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:-4.18|P-value:3.50E-6 ID:SGTC_539|Compound:4859-0462|FD-Score:3.08|P-value:4.81E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:3.64|P-value:4.67E-5 ID:SGTC_581|Compound:0958-0271|FD-Score:4.33|P-value:1.73E-6 ID:SGTC_769|Compound:0302-0231|FD-Score:3.54|P-value:7.43E-5 ID:SGTC_882|Compound:0843-0131|FD-Score:2.98|P-value:6.88E-4 ID:SGTC_918|Compound:glabranin|FD-Score:3.28|P-value:2.15E-4 ID:SGTC_961|Compound:1252-1571|FD-Score:3.02|P-value:5.99E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:3.90|P-value:1.42E-5 ID:SGTC_968|Compound:1309-0633|FD-Score:3.26|P-value:2.33E-4 ID:SGTC_987|Compound:1497-1013|FD-Score:3.08|P-value:4.77E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:3.67|P-value:4.11E-5 ID:SGTC_1017|Compound:4239-0030|FD-Score:-3.55|P-value:6.95E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:-2.96|P-value:7.47E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.22|P-value:2.68E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:5.65|P-value:6.88E-10 ID:SGTC_1091|Compound:telmisartan|FD-Score:4.27|P-value:2.27E-6 ID:SGTC_1096|Compound:rimcazole|FD-Score:3.23|P-value:2.66E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.66|P-value:4.38E-5 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:3.08|P-value:4.75E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:2.91|P-value:9.04E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:5.27|P-value:7.70E-9 ID:SGTC_1235|Compound:benzylparaben|FD-Score:-3.02|P-value:5.79E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-5.31|P-value:5.82E-9 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.19|P-value:3.09E-4 ID:SGTC_1397|Compound:3532-0190|FD-Score:2.92|P-value:8.64E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.32|P-value:1.85E-4 ID:SGTC_1627|Compound:st003713|FD-Score:3.89|P-value:1.53E-5 ID:SGTC_1633|Compound:st005664|FD-Score:3.10|P-value:4.43E-4 ID:SGTC_1637|Compound:st007924|FD-Score:-3.23|P-value:2.63E-4 ID:SGTC_1727|Compound:st034309|FD-Score:2.96|P-value:7.38E-4 ID:SGTC_1784|Compound:st039334|FD-Score:-4.54|P-value:5.52E-7 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.97|P-value:4.97E-8 ID:SGTC_1818|Compound:st052136|FD-Score:2.95|P-value:7.69E-4 ID:SGTC_1958|Compound:st076513|FD-Score:4.16|P-value:4.06E-6 ID:SGTC_1960|Compound:st011366|FD-Score:-2.90|P-value:9.12E-4 ID:SGTC_2036|Compound:5160441|FD-Score:3.65|P-value:4.53E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-5.10|P-value:2.16E-8 ID:SGTC_2055|Compound:5235184|FD-Score:4.87|P-value:8.61E-8 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.34|P-value:1.73E-4 ID:SGTC_2287|Compound:7945887|FD-Score:-3.29|P-value:2.07E-4 ID:SGTC_2288|Compound:7946289|FD-Score:3.95|P-value:1.14E-5 ID:SGTC_2289|Compound:7946404|FD-Score:5.49|P-value:1.94E-9 ID:SGTC_2311|Compound:7694451|FD-Score:3.42|P-value:1.23E-4 ID:SGTC_2440|Compound:5628896|FD-Score:-3.29|P-value:2.02E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:3.74|P-value:2.95E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:8.62|P-value:1.11E-20 ID:SGTC_3062|Compound:9077494|FD-Score:-4.76|P-value:1.58E-7 ID:SGTC_3308|Compound:9128332|FD-Score:3.23|P-value:2.65E-4 ID:SGTC_57|Compound:miconazole|FD-Score:6.31|P-value:6.54E-12 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.43|P-value:1.16E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-4.83|P-value:1.05E-7 ID:SGTC_178|Compound:4092-0806|FD-Score:3.73|P-value:3.17E-5 ID:SGTC_207|Compound:4185-0010|FD-Score:3.49|P-value:8.96E-5 ID:SGTC_252|Compound:miconazole|FD-Score:3.37|P-value:1.48E-4 ID:SGTC_278|Compound:4092-0821|FD-Score:2.94|P-value:8.13E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:3.63|P-value:4.99E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:9.53|P-value:7.30E-25 ID:SGTC_320|Compound:4092-0846|FD-Score:5.51|P-value:1.65E-9 ID:SGTC_372|Compound:0155-0182|FD-Score:-5.00|P-value:3.87E-8 ID:SGTC_383|Compound:2324-0395|FD-Score:-3.98|P-value:9.40E-6 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:3.36|P-value:1.58E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-6.86|P-value:8.90E-14 ID:SGTC_519|Compound:k048-0011|FD-Score:3.21|P-value:2.84E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:-4.18|P-value:3.50E-6 ID:SGTC_539|Compound:4859-0462|FD-Score:3.08|P-value:4.81E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:3.64|P-value:4.67E-5 ID:SGTC_581|Compound:0958-0271|FD-Score:4.33|P-value:1.73E-6 ID:SGTC_769|Compound:0302-0231|FD-Score:3.54|P-value:7.43E-5 ID:SGTC_882|Compound:0843-0131|FD-Score:2.98|P-value:6.88E-4 ID:SGTC_918|Compound:glabranin|FD-Score:3.28|P-value:2.15E-4 ID:SGTC_961|Compound:1252-1571|FD-Score:3.02|P-value:5.99E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:3.90|P-value:1.42E-5 ID:SGTC_968|Compound:1309-0633|FD-Score:3.26|P-value:2.33E-4 ID:SGTC_987|Compound:1497-1013|FD-Score:3.08|P-value:4.77E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:3.67|P-value:4.11E-5 ID:SGTC_1017|Compound:4239-0030|FD-Score:-3.55|P-value:6.95E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:-2.96|P-value:7.47E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.22|P-value:2.68E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:5.65|P-value:6.88E-10 ID:SGTC_1091|Compound:telmisartan|FD-Score:4.27|P-value:2.27E-6 ID:SGTC_1096|Compound:rimcazole|FD-Score:3.23|P-value:2.66E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.66|P-value:4.38E-5 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:3.08|P-value:4.75E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:2.91|P-value:9.04E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:5.27|P-value:7.70E-9 ID:SGTC_1235|Compound:benzylparaben|FD-Score:-3.02|P-value:5.79E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-5.31|P-value:5.82E-9 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.19|P-value:3.09E-4 ID:SGTC_1397|Compound:3532-0190|FD-Score:2.92|P-value:8.64E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.32|P-value:1.85E-4 ID:SGTC_1627|Compound:st003713|FD-Score:3.89|P-value:1.53E-5 ID:SGTC_1633|Compound:st005664|FD-Score:3.10|P-value:4.43E-4 ID:SGTC_1637|Compound:st007924|FD-Score:-3.23|P-value:2.63E-4 ID:SGTC_1727|Compound:st034309|FD-Score:2.96|P-value:7.38E-4 ID:SGTC_1784|Compound:st039334|FD-Score:-4.54|P-value:5.52E-7 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.97|P-value:4.97E-8 ID:SGTC_1818|Compound:st052136|FD-Score:2.95|P-value:7.69E-4 ID:SGTC_1958|Compound:st076513|FD-Score:4.16|P-value:4.06E-6 ID:SGTC_1960|Compound:st011366|FD-Score:-2.90|P-value:9.12E-4 ID:SGTC_2036|Compound:5160441|FD-Score:3.65|P-value:4.53E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-5.10|P-value:2.16E-8 ID:SGTC_2055|Compound:5235184|FD-Score:4.87|P-value:8.61E-8 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.34|P-value:1.73E-4 ID:SGTC_2287|Compound:7945887|FD-Score:-3.29|P-value:2.07E-4 ID:SGTC_2288|Compound:7946289|FD-Score:3.95|P-value:1.14E-5 ID:SGTC_2289|Compound:7946404|FD-Score:5.49|P-value:1.94E-9 ID:SGTC_2311|Compound:7694451|FD-Score:3.42|P-value:1.23E-4 ID:SGTC_2440|Compound:5628896|FD-Score:-3.29|P-value:2.02E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:3.74|P-value:2.95E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:8.62|P-value:1.11E-20 ID:SGTC_3062|Compound:9077494|FD-Score:-4.76|P-value:1.58E-7 ID:SGTC_3308|Compound:9128332|FD-Score:3.23|P-value:2.65E-4

Top fitness defect scores for YMR218C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_312 4092-0653 9.53 6.8 µM 7.30E-25 ergosterol depletion effects on membrane 7 7.70E-22 1.51
2 SGTC_2778 tioconazole 8.62 65.1 nM 1.11E-20 ergosterol depletion effects on membrane 6 3.33E-18 0.15
3 SGTC_57 miconazole 6.31 162.5 nM 6.54E-12 ergosterol depletion effects on membrane 14 1.42E-10 0.49
4 SGTC_1072 oxiconazole 5.65 14.9 nM 6.88E-10 ergosterol depletion effects on membrane 11 8.20E-9 0.15
5 SGTC_320 4092-0846 5.51 5.3 µM 1.65E-9 ergosterol depletion effects on membrane 13 1.76E-8 0.36
6 SGTC_2289 7946404 5.49 165.1 µM 1.94E-9 10 2.02E-8 0.48
7 SGTC_1202 1988-0068 5.27 29.6 µM 7.70E-9 RNA processing & uracil transport 4 6.72E-8 0.15
8 SGTC_1785 bathophenanthroline 4.97 24.0 µM 4.97E-8 heme requiring 13 3.42E-7 0.23
9 SGTC_2055 5235184 4.87 200.0 µM 8.61E-8 ERG2 3 5.52E-7 0.38
10 SGTC_581 0958-0271 4.33 211.0 µM 1.73E-6 3 7.56E-6 0.12
11 SGTC_1091 telmisartan 4.27 47.6 µM 2.27E-6 1 9.60E-6 0.35
12 SGTC_1958 st076513 4.16 41.9 µM 4.06E-6 60S ribosome export 4 1.60E-5 0.16
13 SGTC_2288 7946289 3.95 167.3 µM 1.14E-5 5 3.94E-5 0.14
14 SGTC_966 1300-0360 3.90 30.2 µM 1.42E-5 heme requiring 18 4.77E-5 0.02
15 SGTC_1627 st003713 3.89 25.6 µM 1.53E-5 calcium & mitochondrial duress 7 5.08E-5 0.19
16 SGTC_2498 4'-Methoxychalcone 3.74 54.6 µM 2.95E-5 RPP1 & pyrimidine depletion 4 9.04E-5 0.14
17 SGTC_178 4092-0806 3.73 87.5 µM 3.17E-5 ergosterol depletion effects on membrane 19 9.62E-5 0.01
18 SGTC_1011 4112-3381 3.67 17.4 µM 4.11E-5 2 1.21E-4 0.11
19 SGTC_1098 clofazimine 3.66 15.1 µM 4.38E-5 heme biosynthesis & mitochondrial translocase 31 1.28E-4 0.02
20 SGTC_2036 5160441 3.65 105.0 µM 4.53E-5 NEO1 24 1.31E-4 0.07

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.408 8.67E-135 YIL068C SEC6 het Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
0.352 2.40E-98 YPR055W SEC8 het Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
0.348 4.78E-96 YGL233W SEC15 het Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
0.330 5.02E-86 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.302 2.18E-71 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.298 9.81E-70 YBL010C_p YBL010C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
0.285 1.19E-63 YDR407C TRS120 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.271 1.71E-57 YDR166C SEC5 het Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
0.251 3.56E-49 YMR071C TVP18 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
0.245 8.72E-47 YER008C SEC3 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
0.243 4.46E-46 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.225 1.40E-39 YGL116W CDC20 het Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
0.214 5.09E-36 YLR169W_d YLR169W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.212 1.83E-35 YHL019C APM2 hom Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
0.206 1.58E-33 YJL085W EXO70 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress