YMR223W / UBP8

Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR223W / UBP8

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.28|P-value:2.42E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:5.17|P-value:2.03E-8 ID:SGTC_168|Compound:3970-0862|FD-Score:3.03|P-value:6.22E-4 ID:SGTC_231|Compound:b-estradiol|FD-Score:-3.08|P-value:6.30E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.33|P-value:2.01E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:3.24|P-value:2.83E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.42|P-value:1.36E-4 ID:SGTC_272|Compound:doxorubicin|FD-Score:3.05|P-value:5.84E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:2.98|P-value:7.54E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:4.55|P-value:6.69E-7 ID:SGTC_420|Compound:mitomycin C|FD-Score:-3.29|P-value:2.76E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.57|P-value:8.90E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:4.92|P-value:8.77E-8 ID:SGTC_524|Compound:r073-0010|FD-Score:3.52|P-value:9.18E-5 ID:SGTC_544|Compound:k015-0020|FD-Score:2.93|P-value:9.04E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:3.61|P-value:6.07E-5 ID:SGTC_686|Compound:1319-0111|FD-Score:4.81|P-value:1.68E-7 ID:SGTC_831|Compound:k061-1017|FD-Score:3.26|P-value:2.58E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.22|P-value:3.02E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:2.92|P-value:9.47E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:-3.91|P-value:2.07E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:3.50|P-value:9.77E-5 ID:SGTC_1051|Compound:3253-3903|FD-Score:3.00|P-value:7.10E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.06|P-value:5.61E-4 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.26|P-value:2.64E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:-3.17|P-value:4.45E-4 ID:SGTC_1710|Compound:st031758|FD-Score:3.45|P-value:1.23E-4 ID:SGTC_1774|Compound:st048371|FD-Score:3.10|P-value:4.81E-4 ID:SGTC_1828|Compound:st055811|FD-Score:3.22|P-value:3.09E-4 ID:SGTC_2126|Compound:5251174|FD-Score:4.03|P-value:9.09E-6 ID:SGTC_2338|Compound:7999974|FD-Score:3.46|P-value:1.16E-4 ID:SGTC_2399|Compound:5884133|FD-Score:-5.45|P-value:5.15E-9 ID:SGTC_2402|Compound:5553605|FD-Score:4.64|P-value:4.16E-7 ID:SGTC_2460|Compound:5552655|FD-Score:-2.97|P-value:9.28E-4 ID:SGTC_2475|Compound:5812728|FD-Score:-3.36|P-value:2.10E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:-3.59|P-value:8.40E-5 ID:SGTC_2552|Compound:juarezic acid|FD-Score:-3.33|P-value:2.42E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:-2.99|P-value:8.63E-4 ID:SGTC_2561|Compound:acetosyringone|FD-Score:-4.06|P-value:9.99E-6 ID:SGTC_2612|Compound:dictamnine|FD-Score:-3.23|P-value:3.58E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:2.92|P-value:9.35E-4 ID:SGTC_2713|Compound:st079149|FD-Score:3.09|P-value:5.09E-4 ID:SGTC_2801|Compound:7907800|FD-Score:-3.72|P-value:4.76E-5 ID:SGTC_2807|Compound:7945238|FD-Score:3.49|P-value:1.03E-4 ID:SGTC_2835|Compound:9006588|FD-Score:-4.85|P-value:1.74E-7 ID:SGTC_2851|Compound:9015922|FD-Score:3.41|P-value:1.45E-4 ID:SGTC_2985|Compound:9022375|FD-Score:3.15|P-value:4.05E-4 ID:SGTC_3138|Compound:9097216|FD-Score:-5.26|P-value:1.59E-8 ID:SGTC_3152|Compound:9099253|FD-Score:3.85|P-value:2.15E-5 ID:SGTC_3232|Compound:9132874|FD-Score:-2.97|P-value:9.43E-4 ID:SGTC_3249|Compound:9135183|FD-Score:3.03|P-value:6.20E-4 ID:SGTC_3266|Compound:9136860|FD-Score:3.28|P-value:2.44E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.28|P-value:2.42E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:5.17|P-value:2.03E-8 ID:SGTC_168|Compound:3970-0862|FD-Score:3.03|P-value:6.22E-4 ID:SGTC_231|Compound:b-estradiol|FD-Score:-3.08|P-value:6.30E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.33|P-value:2.01E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:3.24|P-value:2.83E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.42|P-value:1.36E-4 ID:SGTC_272|Compound:doxorubicin|FD-Score:3.05|P-value:5.84E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:2.98|P-value:7.54E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:4.55|P-value:6.69E-7 ID:SGTC_420|Compound:mitomycin C|FD-Score:-3.29|P-value:2.76E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.57|P-value:8.90E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:4.92|P-value:8.77E-8 ID:SGTC_524|Compound:r073-0010|FD-Score:3.52|P-value:9.18E-5 ID:SGTC_544|Compound:k015-0020|FD-Score:2.93|P-value:9.04E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:3.61|P-value:6.07E-5 ID:SGTC_686|Compound:1319-0111|FD-Score:4.81|P-value:1.68E-7 ID:SGTC_831|Compound:k061-1017|FD-Score:3.26|P-value:2.58E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.22|P-value:3.02E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:2.92|P-value:9.47E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:-3.91|P-value:2.07E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:3.50|P-value:9.77E-5 ID:SGTC_1051|Compound:3253-3903|FD-Score:3.00|P-value:7.10E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.06|P-value:5.61E-4 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.26|P-value:2.64E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:-3.17|P-value:4.45E-4 ID:SGTC_1710|Compound:st031758|FD-Score:3.45|P-value:1.23E-4 ID:SGTC_1774|Compound:st048371|FD-Score:3.10|P-value:4.81E-4 ID:SGTC_1828|Compound:st055811|FD-Score:3.22|P-value:3.09E-4 ID:SGTC_2126|Compound:5251174|FD-Score:4.03|P-value:9.09E-6 ID:SGTC_2338|Compound:7999974|FD-Score:3.46|P-value:1.16E-4 ID:SGTC_2399|Compound:5884133|FD-Score:-5.45|P-value:5.15E-9 ID:SGTC_2402|Compound:5553605|FD-Score:4.64|P-value:4.16E-7 ID:SGTC_2460|Compound:5552655|FD-Score:-2.97|P-value:9.28E-4 ID:SGTC_2475|Compound:5812728|FD-Score:-3.36|P-value:2.10E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:-3.59|P-value:8.40E-5 ID:SGTC_2552|Compound:juarezic acid|FD-Score:-3.33|P-value:2.42E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:-2.99|P-value:8.63E-4 ID:SGTC_2561|Compound:acetosyringone|FD-Score:-4.06|P-value:9.99E-6 ID:SGTC_2612|Compound:dictamnine|FD-Score:-3.23|P-value:3.58E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:2.92|P-value:9.35E-4 ID:SGTC_2713|Compound:st079149|FD-Score:3.09|P-value:5.09E-4 ID:SGTC_2801|Compound:7907800|FD-Score:-3.72|P-value:4.76E-5 ID:SGTC_2807|Compound:7945238|FD-Score:3.49|P-value:1.03E-4 ID:SGTC_2835|Compound:9006588|FD-Score:-4.85|P-value:1.74E-7 ID:SGTC_2851|Compound:9015922|FD-Score:3.41|P-value:1.45E-4 ID:SGTC_2985|Compound:9022375|FD-Score:3.15|P-value:4.05E-4 ID:SGTC_3138|Compound:9097216|FD-Score:-5.26|P-value:1.59E-8 ID:SGTC_3152|Compound:9099253|FD-Score:3.85|P-value:2.15E-5 ID:SGTC_3232|Compound:9132874|FD-Score:-2.97|P-value:9.43E-4 ID:SGTC_3249|Compound:9135183|FD-Score:3.03|P-value:6.20E-4 ID:SGTC_3266|Compound:9136860|FD-Score:3.28|P-value:2.44E-4

Top fitness defect scores for YMR223W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 5.17 9.0 µM 2.03E-8 cell wall 265 1.17E-7
2 SGTC_514 4466-0038 4.92 2.6 nM 8.77E-8 cell wall 112 4.32E-7
3 SGTC_686 1319-0111 4.81 510.0 µM 1.68E-7 60S ribosome export 13 7.73E-7
4 SGTC_2402 5553605 4.64 200.0 µM 4.16E-7 11 1.74E-6
5 SGTC_403 arphamenine a 4.55 50.0 µM 6.69E-7 60S ribosome export 9 2.66E-6
6 SGTC_2126 5251174 4.03 33.9 µM 9.09E-6 31 2.76E-5
7 SGTC_3152 9099253 3.85 49.5 µM 2.15E-5 NEO1-PIK1 135 5.96E-5
8 SGTC_576 1073-0113 3.61 22.7 µM 6.07E-5 redox potentiating 75 1.51E-4
9 SGTC_524 r073-0010 3.52 27.8 µM 9.18E-5 65 2.19E-4
10 SGTC_1036 k216-0215 3.50 33.5 µM 9.77E-5 mitochondrial response to ROS 72 2.31E-4
11 SGTC_2807 7945238 3.49 52.0 µM 1.03E-4 41 2.42E-4
12 SGTC_2338 7999974 3.46 200.0 µM 1.16E-4 37 2.70E-4
13 SGTC_1710 st031758 3.45 61.5 µM 1.23E-4 19 2.84E-4
14 SGTC_268 camptothecin 3.42 39.6 µM 1.36E-4 DNA damage response 104 3.11E-4
15 SGTC_2851 9015922 3.41 71.4 µM 1.45E-4 66 3.29E-4
16 SGTC_238 5hr heat shock (37°C) + nsc-638432 3.33 8.9 µM 2.01E-4 heat shock/prefoldin 71 4.41E-4
17 SGTC_6 0844-0013 3.28 19.8 µM 2.42E-4 164 5.21E-4
18 SGTC_3266 9136860 3.28 49.5 µM 2.44E-4 cell wall 68 5.26E-4
19 SGTC_831 k061-1017 3.26 217.0 µM 2.58E-4 translation 105 5.53E-4
20 SGTC_1313 1220-0047 3.26 237.0 µM 2.64E-4 69 5.63E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.232 2.44E-42 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.186 1.73E-27 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.162 4.63E-21 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.161 7.04E-21 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.160 1.09E-20 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.160 1.25E-20 YIL148W RPL40A hom Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.157 6.66E-20 YDR335W MSN5 hom Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm
0.154 3.35E-19 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.151 1.16E-18 YPL047W SGF11 hom Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation
0.148 6.85E-18 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.147 1.40E-17 YNL115C_p YNL115C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene
0.146 1.92E-17 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.146 1.96E-17 YFL056C AAD6 hom Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin
0.146 2.10E-17 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.140 3.58E-16 YKL053W_d YKL053W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1