YMR229C / RRP5

RNA binding protein involved in synthesis of both 18S and 5.8S rRNAs; component of both the ribosomal small subunit (SSU) processosome and the 90S preribosome; has binding preference for single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YMR229C / RRP5

Click on Significant Values for Screen Details ID:SGTC_118|Compound:0083-0097|FD-Score:-2.97|P-value:4.36E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:-2.90|P-value:5.90E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:6.12|P-value:1.15E-12 ID:SGTC_359|Compound:0081-0058|FD-Score:6.30|P-value:2.58E-13 ID:SGTC_362|Compound:0310-0100|FD-Score:2.80|P-value:6.09E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-4.55|P-value:1.50E-7 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-2.90|P-value:5.85E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-5.18|P-value:2.66E-9 ID:SGTC_545|Compound:4218-0007|FD-Score:3.34|P-value:5.73E-5 ID:SGTC_668|Compound:1682-0499|FD-Score:-3.69|P-value:1.74E-5 ID:SGTC_790|Compound:1575-0012|FD-Score:2.73|P-value:7.88E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:-2.78|P-value:9.23E-4 ID:SGTC_844|Compound:0330-0132|FD-Score:4.34|P-value:3.11E-7 ID:SGTC_846|Compound:0335-0850|FD-Score:2.72|P-value:8.21E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:-2.78|P-value:9.51E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:4.93|P-value:7.68E-9 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.29|P-value:7.44E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:-3.51|P-value:4.03E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.98|P-value:2.34E-6 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.02|P-value:3.59E-4 ID:SGTC_1168|Compound:k815-0004|FD-Score:-3.14|P-value:2.16E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:-2.82|P-value:7.89E-4 ID:SGTC_1224|Compound:0823-0416|FD-Score:3.44|P-value:3.62E-5 ID:SGTC_1228|Compound:0124-0032|FD-Score:-3.90|P-value:5.73E-6 ID:SGTC_1235|Compound:benzylparaben|FD-Score:-3.32|P-value:9.73E-5 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:3.50|P-value:2.75E-5 ID:SGTC_1353|Compound:1498-1206|FD-Score:-2.86|P-value:6.91E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.97|P-value:2.50E-6 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.98|P-value:2.35E-6 ID:SGTC_1457|Compound:k015-0032|FD-Score:-3.53|P-value:3.65E-5 ID:SGTC_1629|Compound:st005085|FD-Score:-3.75|P-value:1.28E-5 ID:SGTC_1638|Compound:st008370|FD-Score:-5.05|P-value:6.47E-9 ID:SGTC_1730|Compound:st033808|FD-Score:-3.58|P-value:2.97E-5 ID:SGTC_1763|Compound:st044482|FD-Score:2.89|P-value:4.22E-4 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.25|P-value:8.77E-5 ID:SGTC_1895|Compound:st058434|FD-Score:2.70|P-value:8.81E-4 ID:SGTC_2013|Compound:4024628|FD-Score:3.06|P-value:2.07E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-2.81|P-value:8.20E-4 ID:SGTC_2029|Compound:5175081|FD-Score:2.69|P-value:9.25E-4 ID:SGTC_2098|Compound:5376439|FD-Score:-3.52|P-value:3.79E-5 ID:SGTC_2233|Compound:sulbentine|FD-Score:3.50|P-value:2.76E-5 ID:SGTC_2346|Compound:9031566|FD-Score:2.67|P-value:9.86E-4 ID:SGTC_2486|Compound:5283202|FD-Score:-3.01|P-value:3.67E-4 ID:SGTC_2647|Compound:atorvastatin|FD-Score:2.74|P-value:7.50E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:-2.97|P-value:4.36E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:-2.90|P-value:5.90E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:6.12|P-value:1.15E-12 ID:SGTC_359|Compound:0081-0058|FD-Score:6.30|P-value:2.58E-13 ID:SGTC_362|Compound:0310-0100|FD-Score:2.80|P-value:6.09E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:-4.55|P-value:1.50E-7 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-2.90|P-value:5.85E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-5.18|P-value:2.66E-9 ID:SGTC_545|Compound:4218-0007|FD-Score:3.34|P-value:5.73E-5 ID:SGTC_668|Compound:1682-0499|FD-Score:-3.69|P-value:1.74E-5 ID:SGTC_790|Compound:1575-0012|FD-Score:2.73|P-value:7.88E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:-2.78|P-value:9.23E-4 ID:SGTC_844|Compound:0330-0132|FD-Score:4.34|P-value:3.11E-7 ID:SGTC_846|Compound:0335-0850|FD-Score:2.72|P-value:8.21E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:-2.78|P-value:9.51E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:4.93|P-value:7.68E-9 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.29|P-value:7.44E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:-3.51|P-value:4.03E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.98|P-value:2.34E-6 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.02|P-value:3.59E-4 ID:SGTC_1168|Compound:k815-0004|FD-Score:-3.14|P-value:2.16E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:-2.82|P-value:7.89E-4 ID:SGTC_1224|Compound:0823-0416|FD-Score:3.44|P-value:3.62E-5 ID:SGTC_1228|Compound:0124-0032|FD-Score:-3.90|P-value:5.73E-6 ID:SGTC_1235|Compound:benzylparaben|FD-Score:-3.32|P-value:9.73E-5 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:3.50|P-value:2.75E-5 ID:SGTC_1353|Compound:1498-1206|FD-Score:-2.86|P-value:6.91E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.97|P-value:2.50E-6 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.98|P-value:2.35E-6 ID:SGTC_1457|Compound:k015-0032|FD-Score:-3.53|P-value:3.65E-5 ID:SGTC_1629|Compound:st005085|FD-Score:-3.75|P-value:1.28E-5 ID:SGTC_1638|Compound:st008370|FD-Score:-5.05|P-value:6.47E-9 ID:SGTC_1730|Compound:st033808|FD-Score:-3.58|P-value:2.97E-5 ID:SGTC_1763|Compound:st044482|FD-Score:2.89|P-value:4.22E-4 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.25|P-value:8.77E-5 ID:SGTC_1895|Compound:st058434|FD-Score:2.70|P-value:8.81E-4 ID:SGTC_2013|Compound:4024628|FD-Score:3.06|P-value:2.07E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-2.81|P-value:8.20E-4 ID:SGTC_2029|Compound:5175081|FD-Score:2.69|P-value:9.25E-4 ID:SGTC_2098|Compound:5376439|FD-Score:-3.52|P-value:3.79E-5 ID:SGTC_2233|Compound:sulbentine|FD-Score:3.50|P-value:2.76E-5 ID:SGTC_2346|Compound:9031566|FD-Score:2.67|P-value:9.86E-4 ID:SGTC_2486|Compound:5283202|FD-Score:-3.01|P-value:3.67E-4 ID:SGTC_2647|Compound:atorvastatin|FD-Score:2.74|P-value:7.50E-4

Top fitness defect scores for YMR229C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_359 0081-0058 6.30 15.9 µM 2.58E-13 60S ribosome export 3 1.47E-10 0.47
2 SGTC_309 k408-0051 6.12 1.5 µM 1.15E-12 9 4.67E-10 0.59
3 SGTC_902 1120-0002 4.93 2.1 µM 7.68E-9 azole & statin 13 4.17E-7 0.02
4 SGTC_844 0330-0132 4.34 15.3 µM 3.11E-7 4 7.27E-6 0.81
5 SGTC_1044 1222-0021 3.98 1.8 µM 2.34E-6 heme biosynthesis & mitochondrial translocase 35 3.47E-5 0.31
6 SGTC_1453 4237-0541 3.98 195.0 µM 2.35E-6 heme biosynthesis & mitochondrial translocase 15 3.47E-5 0.14
7 SGTC_1451 4152-0210 3.97 104.0 µM 2.50E-6 2 3.64E-5 0.20
8 SGTC_1292 0kpi-0043 3.50 232.0 µM 2.75E-5 5 2.33E-4 0.08
9 SGTC_2233 sulbentine 3.50 40.4 µM 2.76E-5 43 2.33E-4 0.01
10 SGTC_1224 0823-0416 3.44 294.0 µM 3.62E-5 4 2.89E-4 0.17
11 SGTC_545 4218-0007 3.34 221.0 µM 5.73E-5 11 4.11E-4 0.06
12 SGTC_1038 k292-0204 3.29 154.0 µM 7.44E-5 11 5.04E-4 0.13
13 SGTC_1890 3',4',7',8'-tetrahydroxyflavone 3.25 14.9 µM 8.77E-5 13 5.72E-4 0.03
14 SGTC_2013 4024628 3.06 200.0 µM 2.07E-4 17 0.00111 0.06
15 SGTC_1763 st044482 2.89 94.5 µM 4.22E-4 copper-dependent oxidative stress 18 0.00194 0.01
16 SGTC_362 0310-0100 2.80 9.0 µM 6.09E-4 RPP1 & pyrimidine depletion 24 0.00258 0.21
17 SGTC_2647 atorvastatin 2.74 60.2 µM 7.50E-4 azole & statin 12 0.00303 0.07
18 SGTC_790 1575-0012 2.73 79.1 µM 7.88E-4 TSC3-RPN4 20 0.00315 0.01
19 SGTC_846 0335-0850 2.72 148.0 µM 8.21E-4 ubiquinone biosynthesis & proteasome 19 0.00325 0.13
20 SGTC_1895 st058434 2.70 18.3 µM 8.81E-4 25 0.00343 0.12

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.212 2.58E-35 YJL153C INO1 hom Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element
0.195 5.00E-30 YOL053W AIM39 hom Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss
0.134 5.74E-15 YKR032W_d YKR032W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.111 1.36E-10 YOR329C SCD5 het Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
0.107 4.81E-10 YOR291W YPK9 hom Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
0.106 8.79E-10 YBR083W TEC1 hom Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member
0.104 1.83E-9 YOR275C RIM20 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
0.102 3.61E-9 YGR100W MDR1 hom Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function
0.101 4.77E-9 YDL205C HEM3 het Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
0.101 4.82E-9 YPL019C VTC3 hom Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion
0.100 6.06E-9 YPL064C CWC27 hom Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress
0.097 2.05E-8 YGR174W-A_p YGR174W-A_p hom Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis
0.094 4.26E-8 YJR115W_p YJR115W_p hom Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication
0.092 8.00E-8 YJR017C ESS1 het Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3
0.091 1.18E-7 YLR307C-A_p YLR307C-A_p hom Putative protein of unknown function