YMR238W / DFG5

Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR238W / DFG5

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:4.69|P-value:6.89E-6 ID:SGTC_65|Compound:0798-0905|FD-Score:3.82|P-value:2.04E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.36|P-value:2.71E-5 ID:SGTC_201|Compound:4262-0159|FD-Score:3.74|P-value:2.74E-4 ID:SGTC_349|Compound:k015-0014|FD-Score:-4.86|P-value:2.37E-6 ID:SGTC_350|Compound:k064-0020|FD-Score:-3.59|P-value:3.42E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.27|P-value:3.86E-5 ID:SGTC_358|Compound:0075-0058|FD-Score:7.02|P-value:3.31E-11 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.38|P-value:8.99E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:-4.05|P-value:6.67E-5 ID:SGTC_639|Compound:0833-0042|FD-Score:-3.71|P-value:2.32E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:13.40|P-value:2.64E-36 ID:SGTC_664|Compound:1222-0054|FD-Score:-3.71|P-value:2.27E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:3.59|P-value:4.55E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:3.81|P-value:2.08E-4 ID:SGTC_834|Compound:0312-0008|FD-Score:3.69|P-value:3.17E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:4.37|P-value:2.53E-5 ID:SGTC_892|Compound:0929-0076|FD-Score:3.52|P-value:5.60E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:4.42|P-value:2.13E-5 ID:SGTC_916|Compound:4466-0038|FD-Score:3.39|P-value:8.61E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:4.13|P-value:6.64E-5 ID:SGTC_1007|Compound:4101-0031|FD-Score:3.44|P-value:7.47E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:6.18|P-value:4.67E-9 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.75|P-value:2.64E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:3.55|P-value:5.10E-4 ID:SGTC_1253|Compound:0550-0034|FD-Score:-3.43|P-value:5.90E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:3.44|P-value:7.28E-4 ID:SGTC_1272|Compound:0839-0021|FD-Score:4.84|P-value:3.61E-6 ID:SGTC_1331|Compound:1472-0049|FD-Score:3.63|P-value:3.91E-4 ID:SGTC_1392|Compound:3455-1037|FD-Score:4.09|P-value:7.74E-5 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.74|P-value:2.66E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.93|P-value:1.35E-4 ID:SGTC_1647|Compound:st011722|FD-Score:3.42|P-value:7.78E-4 ID:SGTC_1672|Compound:st016598|FD-Score:3.90|P-value:1.55E-4 ID:SGTC_1682|Compound:st012968|FD-Score:-3.35|P-value:7.59E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.49|P-value:1.60E-5 ID:SGTC_1747|Compound:st037496|FD-Score:6.29|P-value:2.60E-9 ID:SGTC_1767|Compound:st044992|FD-Score:3.59|P-value:4.52E-4 ID:SGTC_1794|Compound:st048918|FD-Score:3.41|P-value:8.00E-4 ID:SGTC_1804|Compound:st050076|FD-Score:3.51|P-value:5.78E-4 ID:SGTC_1812|Compound:st052057|FD-Score:3.50|P-value:6.09E-4 ID:SGTC_1939|Compound:st074455|FD-Score:3.56|P-value:5.05E-4 ID:SGTC_1952|Compound:st077036|FD-Score:3.93|P-value:1.38E-4 ID:SGTC_2007|Compound:4011539|FD-Score:5.15|P-value:8.98E-7 ID:SGTC_2132|Compound:5317594|FD-Score:3.71|P-value:2.95E-4 ID:SGTC_2155|Compound:5570423|FD-Score:4.38|P-value:2.47E-5 ID:SGTC_2164|Compound:5685496|FD-Score:5.16|P-value:8.22E-7 ID:SGTC_2166|Compound:5933061|FD-Score:-3.32|P-value:8.49E-4 ID:SGTC_2241|Compound:6772625|FD-Score:3.67|P-value:3.42E-4 ID:SGTC_2321|Compound:7390090|FD-Score:3.83|P-value:1.95E-4 ID:SGTC_2340|Compound:7987427|FD-Score:3.78|P-value:2.31E-4 ID:SGTC_2346|Compound:9031566|FD-Score:3.89|P-value:1.59E-4 ID:SGTC_2354|Compound:9060492|FD-Score:4.93|P-value:2.39E-6 ID:SGTC_2399|Compound:5884133|FD-Score:10.80|P-value:5.00E-24 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:4.10|P-value:7.22E-5 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:4.69|P-value:6.75E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:3.38|P-value:8.98E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:4.17|P-value:5.53E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.11|P-value:6.85E-9 ID:SGTC_2792|Compound:7535235|FD-Score:-5.16|P-value:5.84E-7 ID:SGTC_2797|Compound:7859098|FD-Score:4.60|P-value:9.96E-6 ID:SGTC_2803|Compound:7707263|FD-Score:5.45|P-value:2.06E-7 ID:SGTC_2922|Compound:7999257|FD-Score:6.15|P-value:5.51E-9 ID:SGTC_2927|Compound:9011802|FD-Score:4.45|P-value:1.85E-5 ID:SGTC_2928|Compound:9011484|FD-Score:4.59|P-value:1.06E-5 ID:SGTC_3014|Compound:9081533|FD-Score:5.57|P-value:1.17E-7 ID:SGTC_3233|Compound:9132340|FD-Score:3.91|P-value:1.45E-4 ID:SGTC_3236|Compound:9132701|FD-Score:6.06|P-value:8.86E-9 ID:SGTC_3326|Compound:9140064|FD-Score:5.10|P-value:1.12E-6 ID:SGTC_57|Compound:miconazole|FD-Score:4.69|P-value:6.89E-6 ID:SGTC_65|Compound:0798-0905|FD-Score:3.82|P-value:2.04E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.36|P-value:2.71E-5 ID:SGTC_201|Compound:4262-0159|FD-Score:3.74|P-value:2.74E-4 ID:SGTC_349|Compound:k015-0014|FD-Score:-4.86|P-value:2.37E-6 ID:SGTC_350|Compound:k064-0020|FD-Score:-3.59|P-value:3.42E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.27|P-value:3.86E-5 ID:SGTC_358|Compound:0075-0058|FD-Score:7.02|P-value:3.31E-11 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.38|P-value:8.99E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:-4.05|P-value:6.67E-5 ID:SGTC_639|Compound:0833-0042|FD-Score:-3.71|P-value:2.32E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:13.40|P-value:2.64E-36 ID:SGTC_664|Compound:1222-0054|FD-Score:-3.71|P-value:2.27E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:3.59|P-value:4.55E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:3.81|P-value:2.08E-4 ID:SGTC_834|Compound:0312-0008|FD-Score:3.69|P-value:3.17E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:4.37|P-value:2.53E-5 ID:SGTC_892|Compound:0929-0076|FD-Score:3.52|P-value:5.60E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:4.42|P-value:2.13E-5 ID:SGTC_916|Compound:4466-0038|FD-Score:3.39|P-value:8.61E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:4.13|P-value:6.64E-5 ID:SGTC_1007|Compound:4101-0031|FD-Score:3.44|P-value:7.47E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:6.18|P-value:4.67E-9 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.75|P-value:2.64E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:3.55|P-value:5.10E-4 ID:SGTC_1253|Compound:0550-0034|FD-Score:-3.43|P-value:5.90E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:3.44|P-value:7.28E-4 ID:SGTC_1272|Compound:0839-0021|FD-Score:4.84|P-value:3.61E-6 ID:SGTC_1331|Compound:1472-0049|FD-Score:3.63|P-value:3.91E-4 ID:SGTC_1392|Compound:3455-1037|FD-Score:4.09|P-value:7.74E-5 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.74|P-value:2.66E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.93|P-value:1.35E-4 ID:SGTC_1647|Compound:st011722|FD-Score:3.42|P-value:7.78E-4 ID:SGTC_1672|Compound:st016598|FD-Score:3.90|P-value:1.55E-4 ID:SGTC_1682|Compound:st012968|FD-Score:-3.35|P-value:7.59E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.49|P-value:1.60E-5 ID:SGTC_1747|Compound:st037496|FD-Score:6.29|P-value:2.60E-9 ID:SGTC_1767|Compound:st044992|FD-Score:3.59|P-value:4.52E-4 ID:SGTC_1794|Compound:st048918|FD-Score:3.41|P-value:8.00E-4 ID:SGTC_1804|Compound:st050076|FD-Score:3.51|P-value:5.78E-4 ID:SGTC_1812|Compound:st052057|FD-Score:3.50|P-value:6.09E-4 ID:SGTC_1939|Compound:st074455|FD-Score:3.56|P-value:5.05E-4 ID:SGTC_1952|Compound:st077036|FD-Score:3.93|P-value:1.38E-4 ID:SGTC_2007|Compound:4011539|FD-Score:5.15|P-value:8.98E-7 ID:SGTC_2132|Compound:5317594|FD-Score:3.71|P-value:2.95E-4 ID:SGTC_2155|Compound:5570423|FD-Score:4.38|P-value:2.47E-5 ID:SGTC_2164|Compound:5685496|FD-Score:5.16|P-value:8.22E-7 ID:SGTC_2166|Compound:5933061|FD-Score:-3.32|P-value:8.49E-4 ID:SGTC_2241|Compound:6772625|FD-Score:3.67|P-value:3.42E-4 ID:SGTC_2321|Compound:7390090|FD-Score:3.83|P-value:1.95E-4 ID:SGTC_2340|Compound:7987427|FD-Score:3.78|P-value:2.31E-4 ID:SGTC_2346|Compound:9031566|FD-Score:3.89|P-value:1.59E-4 ID:SGTC_2354|Compound:9060492|FD-Score:4.93|P-value:2.39E-6 ID:SGTC_2399|Compound:5884133|FD-Score:10.80|P-value:5.00E-24 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:4.10|P-value:7.22E-5 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:4.69|P-value:6.75E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:3.38|P-value:8.98E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:4.17|P-value:5.53E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.11|P-value:6.85E-9 ID:SGTC_2792|Compound:7535235|FD-Score:-5.16|P-value:5.84E-7 ID:SGTC_2797|Compound:7859098|FD-Score:4.60|P-value:9.96E-6 ID:SGTC_2803|Compound:7707263|FD-Score:5.45|P-value:2.06E-7 ID:SGTC_2922|Compound:7999257|FD-Score:6.15|P-value:5.51E-9 ID:SGTC_2927|Compound:9011802|FD-Score:4.45|P-value:1.85E-5 ID:SGTC_2928|Compound:9011484|FD-Score:4.59|P-value:1.06E-5 ID:SGTC_3014|Compound:9081533|FD-Score:5.57|P-value:1.17E-7 ID:SGTC_3233|Compound:9132340|FD-Score:3.91|P-value:1.45E-4 ID:SGTC_3236|Compound:9132701|FD-Score:6.06|P-value:8.86E-9 ID:SGTC_3326|Compound:9140064|FD-Score:5.10|P-value:1.12E-6

Top fitness defect scores for YMR238W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_663 1486-0733 13.40 13.9 µM 2.64E-36 cell wall signaling 7 1.85E-41
2 SGTC_2399 5884133 10.80 200.0 µM 5.00E-24 cell wall signaling 11 2.19E-27
3 SGTC_358 0075-0058 7.02 9.4 µM 3.31E-11 10 1.10E-12
4 SGTC_1747 st037496 6.29 66.6 µM 2.60E-9 3 1.64E-10
5 SGTC_1019 4262-0212 6.18 3.4 µM 4.67E-9 ergosterol depletion effects on membrane 22 3.21E-10
6 SGTC_2922 7999257 6.15 32.5 µM 5.51E-9 1 3.87E-10
7 SGTC_2688 kanamycin b 6.11 96.7 µM 6.85E-9 calcium & mitochondrial duress 153 4.97E-10
8 SGTC_3236 9132701 6.06 49.5 µM 8.86E-9 TSC3-RPN4 4 6.67E-10
9 SGTC_3014 9081533 5.57 49.5 µM 1.17E-7 3 1.29E-8
10 SGTC_2803 7707263 5.45 71.4 µM 2.06E-7 10 2.47E-8
11 SGTC_2164 5685496 5.16 200.0 µM 8.22E-7 9 1.20E-7
12 SGTC_2007 4011539 5.15 71.8 µM 8.98E-7 cell wall signaling 30 1.33E-7
13 SGTC_3326 9140064 5.10 16.4 µM 1.12E-6 11 1.72E-7
14 SGTC_2354 9060492 4.93 162.0 µM 2.39E-6 6 4.09E-7
15 SGTC_1272 0839-0021 4.84 27.0 µM 3.61E-6 9 6.56E-7
16 SGTC_2609 2,3,4-trihydroxy-4'-ethoxybenzophenone 4.69 70.0 µM 6.75E-6 redox potentiating 54 1.35E-6
17 SGTC_57 miconazole 4.69 162.5 nM 6.89E-6 ergosterol depletion effects on membrane 19 1.38E-6
18 SGTC_2797 7859098 4.60 81.8 µM 9.96E-6 18 2.10E-6
19 SGTC_2928 9011484 4.59 9.8 µM 1.06E-5 heme biosynthesis & mitochondrial translocase 28 2.26E-6
20 SGTC_1712 st032288 4.49 53.4 µM 1.60E-5 calcium & mitochondrial duress 144 3.62E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.372 7.63E-111 YAL053W FLC2 hom Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
0.252 1.07E-49 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.236 8.11E-44 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.191 8.07E-29 YNL322C KRE1 hom Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.186 1.65E-27 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.181 3.64E-26 YAL056C-A_d YAL056C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.178 3.54E-25 YNL084C END3 hom EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
0.176 1.25E-24 YJL183W MNN11 hom Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
0.171 1.77E-23 YMR200W ROT1 het Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
0.169 6.24E-23 YKR042W UTH1 hom Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
0.166 4.20E-22 YLR332W MID2 hom O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
0.164 1.36E-21 YAL013W DEP1 hom Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation
0.149 3.89E-18 YEL036C ANP1 hom Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol
0.138 1.50E-15 YHR030C SLT2 hom Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
0.136 2.26E-15 YKL096C-B_p YKL096C-B_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching