YMR240C / CUS1

Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YMR240C / CUS1

Click on Significant Values for Screen Details ID:SGTC_61|Compound:0978-8327|FD-Score:-4.77|P-value:6.81E-7 ID:SGTC_62|Compound:0180-0423|FD-Score:-4.49|P-value:2.79E-6 ID:SGTC_86|Compound:0417-1630|FD-Score:3.70|P-value:1.02E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:4.87|P-value:5.10E-7 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-3.60|P-value:1.31E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:3.21|P-value:6.59E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:3.83|P-value:6.22E-5 ID:SGTC_653|Compound:0203-0016|FD-Score:3.67|P-value:1.15E-4 ID:SGTC_718|Compound:1130-0073|FD-Score:3.11|P-value:9.10E-4 ID:SGTC_823|Compound:0417-1665|FD-Score:-3.25|P-value:4.95E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:3.46|P-value:2.62E-4 ID:SGTC_990|Compound:3985-0011|FD-Score:5.21|P-value:8.29E-8 ID:SGTC_1029|Compound:k015-0030|FD-Score:3.12|P-value:8.98E-4 ID:SGTC_1085|Compound:doxorubicin|FD-Score:3.15|P-value:8.13E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.44|P-value:2.83E-4 ID:SGTC_1408|Compound:3909-7976|FD-Score:3.42|P-value:3.06E-4 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.91|P-value:4.34E-5 ID:SGTC_1578|Compound:josamycin|FD-Score:4.06|P-value:2.31E-5 ID:SGTC_1683|Compound:st010134|FD-Score:3.44|P-value:2.79E-4 ID:SGTC_1887|Compound:st057648|FD-Score:3.76|P-value:8.10E-5 ID:SGTC_1889|Compound:st059080|FD-Score:3.29|P-value:4.90E-4 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.78|P-value:7.63E-5 ID:SGTC_1912|Compound:st060209|FD-Score:3.78|P-value:7.65E-5 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.82|P-value:6.51E-5 ID:SGTC_1954|Compound:st077032|FD-Score:4.32|P-value:7.35E-6 ID:SGTC_2054|Compound:5233173|FD-Score:-3.51|P-value:1.87E-4 ID:SGTC_2337|Compound:9009472|FD-Score:3.54|P-value:1.96E-4 ID:SGTC_2361|Compound:9034121|FD-Score:3.99|P-value:3.19E-5 ID:SGTC_2440|Compound:5628896|FD-Score:3.19|P-value:6.88E-4 ID:SGTC_2450|Compound:5739737|FD-Score:3.72|P-value:9.54E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.55|P-value:1.90E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:5.12|P-value:1.41E-7 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-3.70|P-value:8.97E-5 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-3.19|P-value:6.10E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.55|P-value:1.90E-4 ID:SGTC_2713|Compound:st079149|FD-Score:-3.61|P-value:1.25E-4 ID:SGTC_2763|Compound:lisinopril|FD-Score:4.06|P-value:2.38E-5 ID:SGTC_2782|Compound:7736369|FD-Score:3.20|P-value:6.87E-4 ID:SGTC_2785|Compound:7756463|FD-Score:-3.74|P-value:7.44E-5 ID:SGTC_2791|Compound:5663188|FD-Score:8.96|P-value:9.83E-20 ID:SGTC_2835|Compound:9006588|FD-Score:-4.33|P-value:5.70E-6 ID:SGTC_2856|Compound:9022925|FD-Score:3.48|P-value:2.45E-4 ID:SGTC_3130|Compound:9127414|FD-Score:-4.79|P-value:6.26E-7 ID:SGTC_3137|Compound:9097352|FD-Score:-3.42|P-value:2.67E-4 ID:SGTC_3138|Compound:9097216|FD-Score:-4.54|P-value:2.21E-6 ID:SGTC_3144|Compound:9087057|FD-Score:3.55|P-value:1.90E-4 ID:SGTC_3178|Compound:9107506|FD-Score:3.32|P-value:4.33E-4 ID:SGTC_3328|Compound:9140624|FD-Score:3.09|P-value:9.86E-4 ID:SGTC_61|Compound:0978-8327|FD-Score:-4.77|P-value:6.81E-7 ID:SGTC_62|Compound:0180-0423|FD-Score:-4.49|P-value:2.79E-6 ID:SGTC_86|Compound:0417-1630|FD-Score:3.70|P-value:1.02E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:4.87|P-value:5.10E-7 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-3.60|P-value:1.31E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:3.21|P-value:6.59E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:3.83|P-value:6.22E-5 ID:SGTC_653|Compound:0203-0016|FD-Score:3.67|P-value:1.15E-4 ID:SGTC_718|Compound:1130-0073|FD-Score:3.11|P-value:9.10E-4 ID:SGTC_823|Compound:0417-1665|FD-Score:-3.25|P-value:4.95E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:3.46|P-value:2.62E-4 ID:SGTC_990|Compound:3985-0011|FD-Score:5.21|P-value:8.29E-8 ID:SGTC_1029|Compound:k015-0030|FD-Score:3.12|P-value:8.98E-4 ID:SGTC_1085|Compound:doxorubicin|FD-Score:3.15|P-value:8.13E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:3.44|P-value:2.83E-4 ID:SGTC_1408|Compound:3909-7976|FD-Score:3.42|P-value:3.06E-4 ID:SGTC_1489|Compound:4065-0444|FD-Score:3.91|P-value:4.34E-5 ID:SGTC_1578|Compound:josamycin|FD-Score:4.06|P-value:2.31E-5 ID:SGTC_1683|Compound:st010134|FD-Score:3.44|P-value:2.79E-4 ID:SGTC_1887|Compound:st057648|FD-Score:3.76|P-value:8.10E-5 ID:SGTC_1889|Compound:st059080|FD-Score:3.29|P-value:4.90E-4 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.78|P-value:7.63E-5 ID:SGTC_1912|Compound:st060209|FD-Score:3.78|P-value:7.65E-5 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.82|P-value:6.51E-5 ID:SGTC_1954|Compound:st077032|FD-Score:4.32|P-value:7.35E-6 ID:SGTC_2054|Compound:5233173|FD-Score:-3.51|P-value:1.87E-4 ID:SGTC_2337|Compound:9009472|FD-Score:3.54|P-value:1.96E-4 ID:SGTC_2361|Compound:9034121|FD-Score:3.99|P-value:3.19E-5 ID:SGTC_2440|Compound:5628896|FD-Score:3.19|P-value:6.88E-4 ID:SGTC_2450|Compound:5739737|FD-Score:3.72|P-value:9.54E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.55|P-value:1.90E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:5.12|P-value:1.41E-7 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-3.70|P-value:8.97E-5 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-3.19|P-value:6.10E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.55|P-value:1.90E-4 ID:SGTC_2713|Compound:st079149|FD-Score:-3.61|P-value:1.25E-4 ID:SGTC_2763|Compound:lisinopril|FD-Score:4.06|P-value:2.38E-5 ID:SGTC_2782|Compound:7736369|FD-Score:3.20|P-value:6.87E-4 ID:SGTC_2785|Compound:7756463|FD-Score:-3.74|P-value:7.44E-5 ID:SGTC_2791|Compound:5663188|FD-Score:8.96|P-value:9.83E-20 ID:SGTC_2835|Compound:9006588|FD-Score:-4.33|P-value:5.70E-6 ID:SGTC_2856|Compound:9022925|FD-Score:3.48|P-value:2.45E-4 ID:SGTC_3130|Compound:9127414|FD-Score:-4.79|P-value:6.26E-7 ID:SGTC_3137|Compound:9097352|FD-Score:-3.42|P-value:2.67E-4 ID:SGTC_3138|Compound:9097216|FD-Score:-4.54|P-value:2.21E-6 ID:SGTC_3144|Compound:9087057|FD-Score:3.55|P-value:1.90E-4 ID:SGTC_3178|Compound:9107506|FD-Score:3.32|P-value:4.33E-4 ID:SGTC_3328|Compound:9140624|FD-Score:3.09|P-value:9.86E-4

Top fitness defect scores for YMR240C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2791 5663188 8.96 71.4 µM 9.83E-20 RPP1 & pyrimidine depletion 2 1.55E-19 0.04
2 SGTC_990 3985-0011 5.21 41.3 µM 8.29E-8 amide catabolism 3 9.28E-8 0.60
3 SGTC_2564 betulinic acid 5.12 100.0 µM 1.41E-7 4 1.56E-7 0.07
4 SGTC_116 0155-0182 4.87 31.8 µM 5.10E-7 mitochondrial response to ROS 1 5.59E-7 0.63
5 SGTC_1954 st077032 4.32 68.9 µM 7.35E-6 4 7.83E-6 0.12
6 SGTC_1578 josamycin 4.06 24.2 µM 2.31E-5 5 2.43E-5 0.43
7 SGTC_2763 lisinopril 4.06 49.3 µM 2.38E-5 2 2.50E-5 0.52
8 SGTC_2361 9034121 3.99 116.0 µM 3.19E-5 RSC complex & mRNA processing 20 3.34E-5 0.01
9 SGTC_1489 4065-0444 3.91 182.0 µM 4.34E-5 4 4.54E-5 0.62
10 SGTC_554 4483-2211 3.83 187.0 µM 6.22E-5 2 6.48E-5 0.14
11 SGTC_1918 oxolan-2-ylmethyl 3-bromobenzoate 3.82 70.1 µM 6.51E-5 7 6.77E-5 0.41
12 SGTC_1890 3',4',7',8'-tetrahydroxyflavone 3.78 14.9 µM 7.63E-5 6 7.93E-5 0.04
13 SGTC_1912 st060209 3.78 90.8 µM 7.65E-5 14 7.96E-5 0.07
14 SGTC_1887 st057648 3.76 70.8 µM 8.10E-5 6 8.42E-5 0.12
15 SGTC_2450 5739737 3.72 12.9 µM 9.54E-5 6 9.89E-5 0.17
16 SGTC_86 0417-1630 3.70 471.9 µM 1.02E-4 ubiquinone biosynthesis & proteasome 34 1.06E-4 0.01
17 SGTC_653 0203-0016 3.67 55.2 µM 1.15E-4 4 1.19E-4 0.32
18 SGTC_2627 acetyl isogambogic acid 3.55 100.0 µM 1.90E-4 TSC3-RPN4 9 1.95E-4 0.07
19 SGTC_3144 9087057 3.55 49.5 µM 1.90E-4 6 1.95E-4 0.19
20 SGTC_2519 cryptotanshinone 3.55 88.0 µM 1.90E-4 heme biosynthesis & mitochondrial translocase 26 1.96E-4 0.31

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.104 1.37E-9 YMR133W REC114 hom Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division
0.089 2.19E-7 YOR083W WHI5 hom Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1
0.088 2.98E-7 YLR063W_p YLR063W_p hom Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene
0.088 3.31E-7 YBL049W MOH1 hom Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase
0.085 7.21E-7 YMR023C MSS1 hom Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3
0.081 2.85E-6 YBR070C ALG14 het Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
0.080 3.66E-6 YBR079C RPG1 het eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation
0.079 5.06E-6 YNL279W PRM1 hom Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p
0.078 5.62E-6 YER101C AST2 hom Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication
0.078 5.71E-6 YDR397C NCB2 het Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta
0.078 6.29E-6 YPR182W SMX3 het Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F
0.078 6.29E-6 YCR088W ABP1 hom Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences
0.078 6.43E-6 YBL057C PTH2 hom One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth
0.078 6.74E-6 YDL225W SHS1 hom Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress
0.077 7.33E-6 YJL196C ELO1 hom Elongase I, medium-chain acyl elongase; catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog, FEN1, that arose from the whole genome duplication