YMR246W / FAA4

Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR246W / FAA4

Click on Significant Values for Screen Details ID:SGTC_23|Compound:0213-0011|FD-Score:-3.28|P-value:4.35E-4 ID:SGTC_25|Compound:0118-0223|FD-Score:-3.43|P-value:2.45E-4 ID:SGTC_51|Compound:1013-0126|FD-Score:3.05|P-value:9.24E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:-5.30|P-value:3.51E-8 ID:SGTC_147|Compound:4466-0538|FD-Score:-3.55|P-value:1.57E-4 ID:SGTC_235|Compound:0330-0087|FD-Score:3.71|P-value:7.97E-5 ID:SGTC_344|Compound:k064-0035|FD-Score:3.78|P-value:5.90E-5 ID:SGTC_348|Compound:k064-0012|FD-Score:-5.08|P-value:1.20E-7 ID:SGTC_356|Compound:1634-0012|FD-Score:3.15|P-value:6.66E-4 ID:SGTC_401|Compound:neomycin|FD-Score:3.16|P-value:6.33E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:3.13|P-value:7.12E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.40|P-value:2.67E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.77|P-value:6.01E-5 ID:SGTC_655|Compound:k060-0012|FD-Score:3.56|P-value:1.44E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:3.67|P-value:9.26E-5 ID:SGTC_667|Compound:1498-1044|FD-Score:3.59|P-value:1.29E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:4.69|P-value:8.73E-7 ID:SGTC_780|Compound:0109-0199|FD-Score:3.51|P-value:1.70E-4 ID:SGTC_853|Compound:0419-0306|FD-Score:3.10|P-value:7.85E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:3.07|P-value:8.84E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.10|P-value:7.77E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:3.40|P-value:2.62E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:-3.32|P-value:3.78E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:-5.30|P-value:3.67E-8 ID:SGTC_1828|Compound:st055811|FD-Score:4.28|P-value:6.36E-6 ID:SGTC_1835|Compound:st008666|FD-Score:3.58|P-value:1.31E-4 ID:SGTC_1939|Compound:st074455|FD-Score:5.08|P-value:1.17E-7 ID:SGTC_1941|Compound:st076221|FD-Score:5.46|P-value:1.34E-8 ID:SGTC_1942|Compound:st076330|FD-Score:-3.10|P-value:8.18E-4 ID:SGTC_1950|Compound:st077106|FD-Score:4.28|P-value:6.58E-6 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:4.55|P-value:1.82E-6 ID:SGTC_2132|Compound:5317594|FD-Score:3.71|P-value:7.75E-5 ID:SGTC_2147|Compound:5545932|FD-Score:4.74|P-value:6.79E-7 ID:SGTC_2148|Compound:5546263|FD-Score:3.48|P-value:1.98E-4 ID:SGTC_2252|Compound:omeprazole|FD-Score:3.71|P-value:7.73E-5 ID:SGTC_2253|Compound:7938677|FD-Score:4.37|P-value:4.29E-6 ID:SGTC_2270|Compound:7924575|FD-Score:5.46|P-value:1.34E-8 ID:SGTC_2276|Compound:7947513|FD-Score:8.36|P-value:8.20E-18 ID:SGTC_2305|Compound:7443522|FD-Score:3.61|P-value:1.15E-4 ID:SGTC_2310|Compound:7668677|FD-Score:-4.19|P-value:1.01E-5 ID:SGTC_2315|Compound:7733312|FD-Score:-4.64|P-value:1.20E-6 ID:SGTC_2477|Compound:5967686|FD-Score:4.18|P-value:1.02E-5 ID:SGTC_2480|Compound:5767542|FD-Score:4.23|P-value:8.04E-6 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.64|P-value:1.06E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:-3.07|P-value:8.98E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-6.67|P-value:5.96E-12 ID:SGTC_2815|Compound:7968870|FD-Score:4.78|P-value:5.59E-7 ID:SGTC_2829|Compound:7992743|FD-Score:-3.78|P-value:6.16E-5 ID:SGTC_2928|Compound:9011484|FD-Score:4.15|P-value:1.15E-5 ID:SGTC_2963|Compound:9082507|FD-Score:3.47|P-value:2.04E-4 ID:SGTC_2967|Compound:9089538|FD-Score:3.33|P-value:3.39E-4 ID:SGTC_2989|Compound:9073649|FD-Score:-3.63|P-value:1.15E-4 ID:SGTC_2998|Compound:9066762|FD-Score:-3.64|P-value:1.07E-4 ID:SGTC_3039|Compound:9090270|FD-Score:3.34|P-value:3.26E-4 ID:SGTC_3300|Compound:9124615|FD-Score:4.62|P-value:1.28E-6 ID:SGTC_3350|Compound:9150499|FD-Score:3.56|P-value:1.41E-4 ID:SGTC_23|Compound:0213-0011|FD-Score:-3.28|P-value:4.35E-4 ID:SGTC_25|Compound:0118-0223|FD-Score:-3.43|P-value:2.45E-4 ID:SGTC_51|Compound:1013-0126|FD-Score:3.05|P-value:9.24E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:-5.30|P-value:3.51E-8 ID:SGTC_147|Compound:4466-0538|FD-Score:-3.55|P-value:1.57E-4 ID:SGTC_235|Compound:0330-0087|FD-Score:3.71|P-value:7.97E-5 ID:SGTC_344|Compound:k064-0035|FD-Score:3.78|P-value:5.90E-5 ID:SGTC_348|Compound:k064-0012|FD-Score:-5.08|P-value:1.20E-7 ID:SGTC_356|Compound:1634-0012|FD-Score:3.15|P-value:6.66E-4 ID:SGTC_401|Compound:neomycin|FD-Score:3.16|P-value:6.33E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:3.13|P-value:7.12E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.40|P-value:2.67E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:3.77|P-value:6.01E-5 ID:SGTC_655|Compound:k060-0012|FD-Score:3.56|P-value:1.44E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:3.67|P-value:9.26E-5 ID:SGTC_667|Compound:1498-1044|FD-Score:3.59|P-value:1.29E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:4.69|P-value:8.73E-7 ID:SGTC_780|Compound:0109-0199|FD-Score:3.51|P-value:1.70E-4 ID:SGTC_853|Compound:0419-0306|FD-Score:3.10|P-value:7.85E-4 ID:SGTC_965|Compound:1291-5240|FD-Score:3.07|P-value:8.84E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.10|P-value:7.77E-4 ID:SGTC_1164|Compound:k060-0068|FD-Score:3.40|P-value:2.62E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:-3.32|P-value:3.78E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:-5.30|P-value:3.67E-8 ID:SGTC_1828|Compound:st055811|FD-Score:4.28|P-value:6.36E-6 ID:SGTC_1835|Compound:st008666|FD-Score:3.58|P-value:1.31E-4 ID:SGTC_1939|Compound:st074455|FD-Score:5.08|P-value:1.17E-7 ID:SGTC_1941|Compound:st076221|FD-Score:5.46|P-value:1.34E-8 ID:SGTC_1942|Compound:st076330|FD-Score:-3.10|P-value:8.18E-4 ID:SGTC_1950|Compound:st077106|FD-Score:4.28|P-value:6.58E-6 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:4.55|P-value:1.82E-6 ID:SGTC_2132|Compound:5317594|FD-Score:3.71|P-value:7.75E-5 ID:SGTC_2147|Compound:5545932|FD-Score:4.74|P-value:6.79E-7 ID:SGTC_2148|Compound:5546263|FD-Score:3.48|P-value:1.98E-4 ID:SGTC_2252|Compound:omeprazole|FD-Score:3.71|P-value:7.73E-5 ID:SGTC_2253|Compound:7938677|FD-Score:4.37|P-value:4.29E-6 ID:SGTC_2270|Compound:7924575|FD-Score:5.46|P-value:1.34E-8 ID:SGTC_2276|Compound:7947513|FD-Score:8.36|P-value:8.20E-18 ID:SGTC_2305|Compound:7443522|FD-Score:3.61|P-value:1.15E-4 ID:SGTC_2310|Compound:7668677|FD-Score:-4.19|P-value:1.01E-5 ID:SGTC_2315|Compound:7733312|FD-Score:-4.64|P-value:1.20E-6 ID:SGTC_2477|Compound:5967686|FD-Score:4.18|P-value:1.02E-5 ID:SGTC_2480|Compound:5767542|FD-Score:4.23|P-value:8.04E-6 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.64|P-value:1.06E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:-3.07|P-value:8.98E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-6.67|P-value:5.96E-12 ID:SGTC_2815|Compound:7968870|FD-Score:4.78|P-value:5.59E-7 ID:SGTC_2829|Compound:7992743|FD-Score:-3.78|P-value:6.16E-5 ID:SGTC_2928|Compound:9011484|FD-Score:4.15|P-value:1.15E-5 ID:SGTC_2963|Compound:9082507|FD-Score:3.47|P-value:2.04E-4 ID:SGTC_2967|Compound:9089538|FD-Score:3.33|P-value:3.39E-4 ID:SGTC_2989|Compound:9073649|FD-Score:-3.63|P-value:1.15E-4 ID:SGTC_2998|Compound:9066762|FD-Score:-3.64|P-value:1.07E-4 ID:SGTC_3039|Compound:9090270|FD-Score:3.34|P-value:3.26E-4 ID:SGTC_3300|Compound:9124615|FD-Score:4.62|P-value:1.28E-6 ID:SGTC_3350|Compound:9150499|FD-Score:3.56|P-value:1.41E-4

Top fitness defect scores for YMR246W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2276 7947513 8.36 42.1 µM 8.20E-18 1 3.01E-17
2 SGTC_1941 st076221 5.46 61.8 µM 1.34E-8 sphingolipid biosynthesis & PDR1 36 2.38E-8
3 SGTC_2270 7924575 5.46 90.9 µM 1.34E-8 RPP1 & pyrimidine depletion 4 2.39E-8
4 SGTC_1939 st074455 5.08 53.6 µM 1.17E-7 11 1.94E-7
5 SGTC_2815 7968870 4.78 71.4 µM 5.59E-7 10 8.75E-7
6 SGTC_2147 5545932 4.74 89.7 µM 6.79E-7 ergosterol biosynthesis 6 1.06E-6
7 SGTC_756 0508-1999 4.69 28.3 µM 8.73E-7 RPP1 & pyrimidine depletion 10 1.35E-6
8 SGTC_3300 9124615 4.62 50.5 µM 1.28E-6 9 1.94E-6
9 SGTC_1951 sophoraflavanone G 4.55 9.2 µM 1.82E-6 RNA processing & uracil transport 11 2.74E-6
10 SGTC_2253 7938677 4.37 135.8 µM 4.29E-6 6 6.28E-6
11 SGTC_1828 st055811 4.28 49.0 µM 6.36E-6 4 9.17E-6
12 SGTC_1950 st077106 4.28 7.2 µM 6.58E-6 RNA processing & uracil transport 20 9.48E-6
13 SGTC_2480 5767542 4.23 37.2 µM 8.04E-6 8 1.15E-5
14 SGTC_2477 5967686 4.18 25.2 µM 1.02E-5 21 1.45E-5
15 SGTC_2928 9011484 4.15 9.8 µM 1.15E-5 heme biosynthesis & mitochondrial translocase 33 1.63E-5
16 SGTC_344 k064-0035 3.78 6.4 µM 5.90E-5 60S ribosome export 23 7.89E-5
17 SGTC_451 chloral hydrate 3.77 100.0 µM 6.01E-5 60 8.03E-5
18 SGTC_2252 omeprazole 3.71 400.0 µM 7.73E-5 13 1.03E-4
19 SGTC_2132 5317594 3.71 122.7 µM 7.75E-5 SWF1 & branched chain AA biosynthesis 29 1.03E-4
20 SGTC_235 0330-0087 3.71 5.0 mM 7.97E-5 TSC3-RPN4 33 1.06E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.094 5.37E-8 YNL237W YTP1 hom Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins
0.089 2.32E-7 YGR216C GPI1 het Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs
0.084 1.20E-6 YCL050C APA1 hom AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication
0.081 2.89E-6 YMR078C CTF18 hom Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint
0.080 3.56E-6 YJL070C_p YJL070C_p hom Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication
0.076 1.10E-5 YPL043W NOP4 het Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
0.073 2.33E-5 YMR238W DFG5 hom Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p
0.072 3.05E-5 YPL201C YIG1 hom Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol
0.070 4.95E-5 YPL096W PNG1 hom Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p
0.070 5.37E-5 YFR054C_d YFR054C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.069 6.81E-5 YDR255C RMD5 hom Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain
0.069 7.09E-5 YLR409C UTP21 het Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma
0.068 8.82E-5 YOL162W_p YOL162W_p hom Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family
0.066 1.27E-4 YKL174C TPO5 hom Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles
0.066 1.38E-4 YBR182C-A_p YBR182C-A_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching