YMR247C / RKR1

RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR247C / RKR1

Click on Significant Values for Screen Details ID:SGTC_105|Compound:0111-0055|FD-Score:3.75|P-value:2.47E-5 ID:SGTC_107|Compound:0358-0022|FD-Score:5.37|P-value:3.06E-9 ID:SGTC_113|Compound:0099-0221|FD-Score:5.12|P-value:1.56E-8 ID:SGTC_119|Compound:0121-0043|FD-Score:-3.08|P-value:4.30E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:5.24|P-value:7.10E-9 ID:SGTC_140|Compound:0062-0056|FD-Score:3.37|P-value:1.36E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:3.54|P-value:6.52E-5 ID:SGTC_413|Compound:propylparaben|FD-Score:-4.08|P-value:4.93E-6 ID:SGTC_520|Compound:3584-0023|FD-Score:5.25|P-value:6.74E-9 ID:SGTC_534|Compound:k292-0788|FD-Score:3.71|P-value:3.02E-5 ID:SGTC_538|Compound:2922-0838|FD-Score:4.35|P-value:1.27E-6 ID:SGTC_556|Compound:4058-0063|FD-Score:3.27|P-value:2.07E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:3.12|P-value:3.70E-4 ID:SGTC_631|Compound:k072-0230|FD-Score:3.32|P-value:1.63E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:-4.38|P-value:1.02E-6 ID:SGTC_731|Compound:3966-0296|FD-Score:4.35|P-value:1.28E-6 ID:SGTC_884|Compound:0851-0618|FD-Score:2.90|P-value:8.46E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:2.94|P-value:7.51E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:3.16|P-value:3.22E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:3.50|P-value:7.82E-5 ID:SGTC_1031|Compound:k035-0009|FD-Score:7.01|P-value:1.70E-14 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.33|P-value:1.62E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.43|P-value:1.06E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.62|P-value:4.55E-5 ID:SGTC_1266|Compound:0828-0227|FD-Score:-3.32|P-value:1.64E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:4.32|P-value:1.52E-6 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:4.36|P-value:1.19E-6 ID:SGTC_1690|Compound:st023502|FD-Score:-3.05|P-value:4.69E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.39|P-value:1.22E-4 ID:SGTC_1860|Compound:st056228|FD-Score:2.96|P-value:6.91E-4 ID:SGTC_1889|Compound:st059080|FD-Score:-3.47|P-value:8.48E-5 ID:SGTC_2052|Compound:5265182|FD-Score:6.42|P-value:1.87E-12 ID:SGTC_2057|Compound:5236571|FD-Score:9.07|P-value:5.02E-23 ID:SGTC_2059|Compound:5238311|FD-Score:-4.10|P-value:4.55E-6 ID:SGTC_2146|Compound:5545642|FD-Score:2.97|P-value:6.62E-4 ID:SGTC_2201|Compound:6605497|FD-Score:3.02|P-value:5.39E-4 ID:SGTC_2348|Compound:9022543|FD-Score:3.72|P-value:2.84E-5 ID:SGTC_2354|Compound:9060492|FD-Score:-3.32|P-value:1.60E-4 ID:SGTC_2357|Compound:9067248|FD-Score:3.89|P-value:1.29E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:-4.29|P-value:1.68E-6 ID:SGTC_2416|Compound:tofa|FD-Score:-3.11|P-value:3.72E-4 ID:SGTC_2421|Compound:5690543|FD-Score:2.87|P-value:9.70E-4 ID:SGTC_2428|Compound:5615643|FD-Score:7.37|P-value:7.44E-16 ID:SGTC_2431|Compound:5490079|FD-Score:5.78|P-value:2.00E-10 ID:SGTC_2469|Compound:5655019|FD-Score:3.41|P-value:1.12E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:-3.73|P-value:2.61E-5 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:4.71|P-value:1.79E-7 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:3.04|P-value:5.00E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.64|P-value:4.04E-5 ID:SGTC_2587|Compound:piperic acid|FD-Score:-3.24|P-value:2.27E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:5.03|P-value:2.71E-8 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:2.99|P-value:6.24E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:-3.03|P-value:5.05E-4 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-4.04|P-value:6.13E-6 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.34|P-value:1.35E-6 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.52|P-value:6.91E-5 ID:SGTC_2754|Compound:granisetron|FD-Score:4.36|P-value:1.21E-6 ID:SGTC_2809|Compound:7964133|FD-Score:3.44|P-value:1.01E-4 ID:SGTC_2819|Compound:7996655|FD-Score:4.39|P-value:1.04E-6 ID:SGTC_2824|Compound:7998404|FD-Score:-3.28|P-value:1.91E-4 ID:SGTC_2829|Compound:7992743|FD-Score:3.29|P-value:1.87E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.69|P-value:3.28E-5 ID:SGTC_2869|Compound:9037858|FD-Score:-2.88|P-value:9.12E-4 ID:SGTC_3033|Compound:9090887|FD-Score:3.05|P-value:4.90E-4 ID:SGTC_3198|Compound:9112019|FD-Score:-3.53|P-value:6.42E-5 ID:SGTC_3314|Compound:9135860|FD-Score:5.66|P-value:4.70E-10 ID:SGTC_105|Compound:0111-0055|FD-Score:3.75|P-value:2.47E-5 ID:SGTC_107|Compound:0358-0022|FD-Score:5.37|P-value:3.06E-9 ID:SGTC_113|Compound:0099-0221|FD-Score:5.12|P-value:1.56E-8 ID:SGTC_119|Compound:0121-0043|FD-Score:-3.08|P-value:4.30E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:5.24|P-value:7.10E-9 ID:SGTC_140|Compound:0062-0056|FD-Score:3.37|P-value:1.36E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:3.54|P-value:6.52E-5 ID:SGTC_413|Compound:propylparaben|FD-Score:-4.08|P-value:4.93E-6 ID:SGTC_520|Compound:3584-0023|FD-Score:5.25|P-value:6.74E-9 ID:SGTC_534|Compound:k292-0788|FD-Score:3.71|P-value:3.02E-5 ID:SGTC_538|Compound:2922-0838|FD-Score:4.35|P-value:1.27E-6 ID:SGTC_556|Compound:4058-0063|FD-Score:3.27|P-value:2.07E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:3.12|P-value:3.70E-4 ID:SGTC_631|Compound:k072-0230|FD-Score:3.32|P-value:1.63E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:-4.38|P-value:1.02E-6 ID:SGTC_731|Compound:3966-0296|FD-Score:4.35|P-value:1.28E-6 ID:SGTC_884|Compound:0851-0618|FD-Score:2.90|P-value:8.46E-4 ID:SGTC_934|Compound:2950-0684|FD-Score:2.94|P-value:7.51E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:3.16|P-value:3.22E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:3.50|P-value:7.82E-5 ID:SGTC_1031|Compound:k035-0009|FD-Score:7.01|P-value:1.70E-14 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.33|P-value:1.62E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.43|P-value:1.06E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.62|P-value:4.55E-5 ID:SGTC_1266|Compound:0828-0227|FD-Score:-3.32|P-value:1.64E-4 ID:SGTC_1479|Compound:0549-0158|FD-Score:4.32|P-value:1.52E-6 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:4.36|P-value:1.19E-6 ID:SGTC_1690|Compound:st023502|FD-Score:-3.05|P-value:4.69E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.39|P-value:1.22E-4 ID:SGTC_1860|Compound:st056228|FD-Score:2.96|P-value:6.91E-4 ID:SGTC_1889|Compound:st059080|FD-Score:-3.47|P-value:8.48E-5 ID:SGTC_2052|Compound:5265182|FD-Score:6.42|P-value:1.87E-12 ID:SGTC_2057|Compound:5236571|FD-Score:9.07|P-value:5.02E-23 ID:SGTC_2059|Compound:5238311|FD-Score:-4.10|P-value:4.55E-6 ID:SGTC_2146|Compound:5545642|FD-Score:2.97|P-value:6.62E-4 ID:SGTC_2201|Compound:6605497|FD-Score:3.02|P-value:5.39E-4 ID:SGTC_2348|Compound:9022543|FD-Score:3.72|P-value:2.84E-5 ID:SGTC_2354|Compound:9060492|FD-Score:-3.32|P-value:1.60E-4 ID:SGTC_2357|Compound:9067248|FD-Score:3.89|P-value:1.29E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:-4.29|P-value:1.68E-6 ID:SGTC_2416|Compound:tofa|FD-Score:-3.11|P-value:3.72E-4 ID:SGTC_2421|Compound:5690543|FD-Score:2.87|P-value:9.70E-4 ID:SGTC_2428|Compound:5615643|FD-Score:7.37|P-value:7.44E-16 ID:SGTC_2431|Compound:5490079|FD-Score:5.78|P-value:2.00E-10 ID:SGTC_2469|Compound:5655019|FD-Score:3.41|P-value:1.12E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:-3.73|P-value:2.61E-5 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:4.71|P-value:1.79E-7 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:3.04|P-value:5.00E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.64|P-value:4.04E-5 ID:SGTC_2587|Compound:piperic acid|FD-Score:-3.24|P-value:2.27E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:5.03|P-value:2.71E-8 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:2.99|P-value:6.24E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:-3.03|P-value:5.05E-4 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-4.04|P-value:6.13E-6 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.34|P-value:1.35E-6 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.52|P-value:6.91E-5 ID:SGTC_2754|Compound:granisetron|FD-Score:4.36|P-value:1.21E-6 ID:SGTC_2809|Compound:7964133|FD-Score:3.44|P-value:1.01E-4 ID:SGTC_2819|Compound:7996655|FD-Score:4.39|P-value:1.04E-6 ID:SGTC_2824|Compound:7998404|FD-Score:-3.28|P-value:1.91E-4 ID:SGTC_2829|Compound:7992743|FD-Score:3.29|P-value:1.87E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.69|P-value:3.28E-5 ID:SGTC_2869|Compound:9037858|FD-Score:-2.88|P-value:9.12E-4 ID:SGTC_3033|Compound:9090887|FD-Score:3.05|P-value:4.90E-4 ID:SGTC_3198|Compound:9112019|FD-Score:-3.53|P-value:6.42E-5 ID:SGTC_3314|Compound:9135860|FD-Score:5.66|P-value:4.70E-10

Top fitness defect scores for YMR247C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2057 5236571 9.07 38.3 µM 5.02E-23 RPP1 & pyrimidine depletion 2 6.01E-20
2 SGTC_2428 5615643 7.37 7.8 µM 7.44E-16 4 8.24E-14
3 SGTC_1031 k035-0009 7.01 16.0 µM 1.70E-14 1 1.20E-12
4 SGTC_2052 5265182 6.42 7.1 µM 1.87E-12 1 6.73E-11
5 SGTC_2431 5490079 5.78 200.0 µM 2.00E-10 8 3.70E-9
6 SGTC_3314 9135860 5.66 59.4 µM 4.70E-10 6 7.68E-9
7 SGTC_107 0358-0022 5.37 2.7 µM 3.06E-9 SWF1 & branched chain AA biosynthesis 19 3.83E-8
8 SGTC_520 3584-0023 5.25 682.0 µM 6.74E-9 8 7.55E-8
9 SGTC_135 0933-0004 5.24 26.4 µM 7.10E-9 60S ribosome export 7 7.89E-8
10 SGTC_113 0099-0221 5.12 2.5 µM 1.56E-8 9 1.56E-7
11 SGTC_2588 indole-3-carbinol 5.03 100.0 µM 2.71E-8 20 2.49E-7
12 SGTC_2499 avocadenofuran 4.71 51.5 µM 1.79E-7 19 1.26E-6
13 SGTC_2819 7996655 4.39 46.4 µM 1.04E-6 13 5.73E-6
14 SGTC_1585 2',4'-dihydroxychalcone 4.36 10.4 µM 1.19E-6 19 6.40E-6
15 SGTC_2754 granisetron 4.36 64.0 µM 1.21E-6 24 6.53E-6
16 SGTC_538 2922-0838 4.35 53.3 µM 1.27E-6 Golgi 24 6.80E-6
17 SGTC_731 3966-0296 4.35 387.0 µM 1.28E-6 9 6.83E-6
18 SGTC_2636 apigenin dimethyl ether 4.34 100.0 µM 1.35E-6 31 7.15E-6
19 SGTC_1479 0549-0158 4.32 134.0 µM 1.52E-6 13 7.94E-6
20 SGTC_2357 9067248 3.89 200.0 µM 1.29E-5 16 4.99E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.128 1.22E-13 YNL118C DCP2 het Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress
0.107 4.98E-10 YBR257W POP4 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.104 1.52E-9 YDL140C RPO21 het RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
0.098 1.08E-8 YKR086W PRP16 het DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step
0.095 3.62E-8 YHR106W TRR2 hom Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication
0.095 4.19E-8 YHR066W SSF1 hom Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication
0.093 6.71E-8 YDR075W PPH3 hom Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes
0.089 2.41E-7 YGR023W MTL1 hom Putative plasma membrane sensor, involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p
0.088 3.57E-7 YML027W YOX1 hom Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication
0.087 4.59E-7 YOR181W LAS17 het Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)
0.086 5.23E-7 YNL206C RTT106 hom Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition
0.086 5.88E-7 YGR278W CWC22 het Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein
0.085 9.12E-7 YDR128W MTC5 hom Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress
0.084 9.63E-7 YEL068C_p YEL068C_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.082 1.77E-6 YGR227W DIE2 hom Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1