YMR250W / GAD1

Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR250W / GAD1

Click on Significant Values for Screen Details ID:SGTC_20|Compound:0481-0013|FD-Score:3.18|P-value:9.44E-4 ID:SGTC_74|Compound:000s-0487|FD-Score:3.65|P-value:1.87E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:3.33|P-value:5.76E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:4.61|P-value:3.43E-6 ID:SGTC_192|Compound:3455-0609|FD-Score:3.74|P-value:1.33E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:-3.32|P-value:5.72E-4 ID:SGTC_222|Compound:nsc-310342|FD-Score:-3.31|P-value:5.77E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:-3.25|P-value:7.28E-4 ID:SGTC_375|Compound:3970-0961|FD-Score:3.30|P-value:6.36E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-4.33|P-value:1.07E-5 ID:SGTC_430|Compound:0958-0115|FD-Score:5.24|P-value:1.52E-7 ID:SGTC_444|Compound:curcumin|FD-Score:3.61|P-value:2.15E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:4.48|P-value:6.00E-6 ID:SGTC_552|Compound:k242-0005|FD-Score:-3.24|P-value:7.46E-4 ID:SGTC_560|Compound:2332-1943|FD-Score:4.25|P-value:1.69E-5 ID:SGTC_581|Compound:0958-0271|FD-Score:4.16|P-value:2.46E-5 ID:SGTC_751|Compound:0833-0568|FD-Score:3.67|P-value:1.69E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:3.65|P-value:1.87E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:4.02|P-value:4.30E-5 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.44|P-value:3.96E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:4.03|P-value:4.23E-5 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.50|P-value:3.15E-4 ID:SGTC_1352|Compound:1496-0991|FD-Score:3.97|P-value:5.28E-5 ID:SGTC_1358|Compound:1509-0022|FD-Score:3.73|P-value:1.35E-4 ID:SGTC_1609|Compound:st001574|FD-Score:3.19|P-value:9.32E-4 ID:SGTC_1658|Compound:st012944|FD-Score:6.41|P-value:1.92E-10 ID:SGTC_1868|Compound:st057644|FD-Score:5.56|P-value:2.80E-8 ID:SGTC_1909|Compound:st060837|FD-Score:-3.50|P-value:2.99E-4 ID:SGTC_1914|Compound:st062367|FD-Score:3.40|P-value:4.54E-4 ID:SGTC_1949|Compound:st076024|FD-Score:3.43|P-value:4.10E-4 ID:SGTC_2063|Compound:5246145|FD-Score:3.75|P-value:1.24E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.65|P-value:1.86E-4 ID:SGTC_2068|Compound:5226020|FD-Score:4.41|P-value:8.15E-6 ID:SGTC_2069|Compound:5228469|FD-Score:6.30|P-value:3.77E-10 ID:SGTC_2070|Compound:5230947|FD-Score:4.79|P-value:1.47E-6 ID:SGTC_2130|Compound:NSC 7|FD-Score:5.37|P-value:7.85E-8 ID:SGTC_2146|Compound:5545642|FD-Score:4.22|P-value:1.88E-5 ID:SGTC_2189|Compound:6047310|FD-Score:4.15|P-value:2.54E-5 ID:SGTC_2314|Compound:7727897|FD-Score:4.18|P-value:2.25E-5 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:3.19|P-value:9.16E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:3.34|P-value:5.49E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:3.70|P-value:1.53E-4 ID:SGTC_2646|Compound:retinol|FD-Score:3.18|P-value:9.49E-4 ID:SGTC_2699|Compound:st077704|FD-Score:4.18|P-value:2.24E-5 ID:SGTC_2948|Compound:9070189|FD-Score:3.66|P-value:1.75E-4 ID:SGTC_3047|Compound:9092122|FD-Score:-3.49|P-value:3.05E-4 ID:SGTC_3057|Compound:9095946|FD-Score:3.95|P-value:5.68E-5 ID:SGTC_3272|Compound:9137413|FD-Score:3.79|P-value:1.07E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:3.18|P-value:9.44E-4 ID:SGTC_74|Compound:000s-0487|FD-Score:3.65|P-value:1.87E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:3.33|P-value:5.76E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:4.61|P-value:3.43E-6 ID:SGTC_192|Compound:3455-0609|FD-Score:3.74|P-value:1.33E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:-3.32|P-value:5.72E-4 ID:SGTC_222|Compound:nsc-310342|FD-Score:-3.31|P-value:5.77E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:-3.25|P-value:7.28E-4 ID:SGTC_375|Compound:3970-0961|FD-Score:3.30|P-value:6.36E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-4.33|P-value:1.07E-5 ID:SGTC_430|Compound:0958-0115|FD-Score:5.24|P-value:1.52E-7 ID:SGTC_444|Compound:curcumin|FD-Score:3.61|P-value:2.15E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:4.48|P-value:6.00E-6 ID:SGTC_552|Compound:k242-0005|FD-Score:-3.24|P-value:7.46E-4 ID:SGTC_560|Compound:2332-1943|FD-Score:4.25|P-value:1.69E-5 ID:SGTC_581|Compound:0958-0271|FD-Score:4.16|P-value:2.46E-5 ID:SGTC_751|Compound:0833-0568|FD-Score:3.67|P-value:1.69E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:3.65|P-value:1.87E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:4.02|P-value:4.30E-5 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.44|P-value:3.96E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:4.03|P-value:4.23E-5 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.50|P-value:3.15E-4 ID:SGTC_1352|Compound:1496-0991|FD-Score:3.97|P-value:5.28E-5 ID:SGTC_1358|Compound:1509-0022|FD-Score:3.73|P-value:1.35E-4 ID:SGTC_1609|Compound:st001574|FD-Score:3.19|P-value:9.32E-4 ID:SGTC_1658|Compound:st012944|FD-Score:6.41|P-value:1.92E-10 ID:SGTC_1868|Compound:st057644|FD-Score:5.56|P-value:2.80E-8 ID:SGTC_1909|Compound:st060837|FD-Score:-3.50|P-value:2.99E-4 ID:SGTC_1914|Compound:st062367|FD-Score:3.40|P-value:4.54E-4 ID:SGTC_1949|Compound:st076024|FD-Score:3.43|P-value:4.10E-4 ID:SGTC_2063|Compound:5246145|FD-Score:3.75|P-value:1.24E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.65|P-value:1.86E-4 ID:SGTC_2068|Compound:5226020|FD-Score:4.41|P-value:8.15E-6 ID:SGTC_2069|Compound:5228469|FD-Score:6.30|P-value:3.77E-10 ID:SGTC_2070|Compound:5230947|FD-Score:4.79|P-value:1.47E-6 ID:SGTC_2130|Compound:NSC 7|FD-Score:5.37|P-value:7.85E-8 ID:SGTC_2146|Compound:5545642|FD-Score:4.22|P-value:1.88E-5 ID:SGTC_2189|Compound:6047310|FD-Score:4.15|P-value:2.54E-5 ID:SGTC_2314|Compound:7727897|FD-Score:4.18|P-value:2.25E-5 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:3.19|P-value:9.16E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:3.34|P-value:5.49E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:3.70|P-value:1.53E-4 ID:SGTC_2646|Compound:retinol|FD-Score:3.18|P-value:9.49E-4 ID:SGTC_2699|Compound:st077704|FD-Score:4.18|P-value:2.24E-5 ID:SGTC_2948|Compound:9070189|FD-Score:3.66|P-value:1.75E-4 ID:SGTC_3047|Compound:9092122|FD-Score:-3.49|P-value:3.05E-4 ID:SGTC_3057|Compound:9095946|FD-Score:3.95|P-value:5.68E-5 ID:SGTC_3272|Compound:9137413|FD-Score:3.79|P-value:1.07E-4

Top fitness defect scores for YMR250W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1658 st012944 6.41 20.0 µM 1.92E-10 iron homeostasis 25 7.38E-11
2 SGTC_2069 5228469 6.30 200.0 µM 3.77E-10 1 1.50E-10
3 SGTC_1868 st057644 5.56 42.7 µM 2.80E-8 13 1.35E-8
4 SGTC_2130 NSC 7 5.37 2.5 µM 7.85E-8 copper-dependent oxidative stress 9 3.96E-8
5 SGTC_430 0958-0115 5.24 16.4 µM 1.52E-7 9 7.90E-8
6 SGTC_2070 5230947 4.79 200.0 µM 1.47E-6 2 8.49E-7
7 SGTC_121 0557-0495 4.61 61.3 µM 3.43E-6 calcium & mitochondrial duress 37 2.05E-6
8 SGTC_550 4123-0385 4.48 25.7 µM 6.00E-6 TSC3-RPN4 16 3.68E-6
9 SGTC_2068 5226020 4.41 147.0 µM 8.15E-6 22 5.07E-6
10 SGTC_560 2332-1943 4.25 155.0 µM 1.69E-5 5 1.08E-5
11 SGTC_2146 5545642 4.22 200.0 µM 1.88E-5 9 1.22E-5
12 SGTC_2699 st077704 4.18 54.6 µM 2.24E-5 endomembrane recycling 43 1.45E-5
13 SGTC_2314 7727897 4.18 200.0 µM 2.25E-5 17 1.47E-5
14 SGTC_581 0958-0271 4.16 211.0 µM 2.46E-5 43 1.61E-5
15 SGTC_2189 6047310 4.15 154.4 µM 2.54E-5 RPP1 & pyrimidine depletion 15 1.66E-5
16 SGTC_1335 1486-0900 4.03 202.0 µM 4.23E-5 23 2.83E-5
17 SGTC_1095 3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran 4.02 15.5 µM 4.30E-5 24 2.88E-5
18 SGTC_1352 1496-0991 3.97 99.0 µM 5.28E-5 tubulin folding & SWR complex 34 3.57E-5
19 SGTC_3057 9095946 3.95 49.5 µM 5.68E-5 Golgi 14 3.85E-5
20 SGTC_3272 9137413 3.79 49.5 µM 1.07E-4 18 7.49E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.099 8.98E-9 YDR322C-A TIM11 hom Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase
0.099 8.89E-9 YLR120C YPS1 hom Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
0.096 2.59E-8 YDR032C PST2 hom Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
0.092 1.01E-7 YGL051W MST27 hom Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles
0.090 1.81E-7 YPR010C RPA135 het RNA polymerase I second largest subunit A135
0.082 1.90E-6 YBL106C SRO77 hom Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication
0.081 2.80E-6 YDR299W BFR2 het Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
0.079 4.68E-6 YBR204C LDH1 hom Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif)
0.077 7.67E-6 YMR057C_d YMR057C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1
0.074 1.87E-5 YBR229C ROT2 hom Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
0.072 3.00E-5 YER176W ECM32 hom DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes
0.070 4.43E-5 YPL064C CWC27 hom Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress
0.070 4.75E-5 YBR247C ENP1 het Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus
0.069 5.78E-5 YPR050C_d YPR050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W
0.069 6.84E-5 YGL201C MCM6 het Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p