HIPHOP chemogenomics database

YMR250W / GAD1

Glutamate decarboxylase, converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress

Zygosity: Homozygous strain

fixedexpanded
Profile for YMR250W / GAD1

Click on Significant Values for Screen Details

Top fitness defect scores for YMR250W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1658	st012944	6.41	20.0 µM	1.92E-10	iron homeostasis	25	7.38E-11
2	SGTC_2069	5228469	6.30	200.0 µM	3.77E-10		1	1.50E-10
3	SGTC_1868	st057644	5.56	42.7 µM	2.80E-8		13	1.35E-8
4	SGTC_2130	NSC 7	5.37	2.5 µM	7.85E-8	copper-dependent oxidative stress	9	3.96E-8
5	SGTC_430	0958-0115	5.24	16.4 µM	1.52E-7		9	7.90E-8
6	SGTC_2070	5230947	4.79	200.0 µM	1.47E-6		2	8.49E-7
7	SGTC_121	0557-0495	4.61	61.3 µM	3.43E-6	calcium & mitochondrial duress	37	2.05E-6
8	SGTC_550	4123-0385	4.48	25.7 µM	6.00E-6	TSC3-RPN4	16	3.68E-6
9	SGTC_2068	5226020	4.41	147.0 µM	8.15E-6		22	5.07E-6
10	SGTC_560	2332-1943	4.25	155.0 µM	1.69E-5		5	1.08E-5
11	SGTC_2146	5545642	4.22	200.0 µM	1.88E-5		9	1.22E-5
12	SGTC_2699	st077704	4.18	54.6 µM	2.24E-5	endomembrane recycling	43	1.45E-5
13	SGTC_2314	7727897	4.18	200.0 µM	2.25E-5		17	1.47E-5
14	SGTC_581	0958-0271	4.16	211.0 µM	2.46E-5		43	1.61E-5
15	SGTC_2189	6047310	4.15	154.4 µM	2.54E-5	RPP1 & pyrimidine depletion	15	1.66E-5
16	SGTC_1335	1486-0900	4.03	202.0 µM	4.23E-5		23	2.83E-5
17	SGTC_1095	3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran	4.02	15.5 µM	4.30E-5		24	2.88E-5
18	SGTC_1352	1496-0991	3.97	99.0 µM	5.28E-5	tubulin folding & SWR complex	34	3.57E-5
19	SGTC_3057	9095946	3.95	49.5 µM	5.68E-5	Golgi	14	3.85E-5
20	SGTC_3272	9137413	3.79	49.5 µM	1.07E-4		18	7.49E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.099	8.98E-9	YDR322C-A	TIM11	hom	Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase
0.099	8.89E-9	YLR120C	YPS1	hom	Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
0.096	2.59E-8	YDR032C	PST2	hom	Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
0.092	1.01E-7	YGL051W	MST27	hom	Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles
0.090	1.81E-7	YPR010C	RPA135	het	RNA polymerase I second largest subunit A135
0.082	1.90E-6	YBL106C	SRO77	hom	Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication
0.081	2.80E-6	YDR299W	BFR2	het	Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
0.079	4.68E-6	YBR204C	LDH1	hom	Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif)
0.077	7.67E-6	YMR057C_d	YMR057C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1
0.074	1.87E-5	YBR229C	ROT2	hom	Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
0.072	3.00E-5	YER176W	ECM32	hom	DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes
0.070	4.43E-5	YPL064C	CWC27	hom	Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress
0.070	4.75E-5	YBR247C	ENP1	het	Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus
0.069	5.78E-5	YPR050C_d	YPR050C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W
0.069	6.84E-5	YGL201C	MCM6	het	Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p