YMR258C / ROY1

GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR258C / ROY1

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:4.51|P-value:4.03E-6 ID:SGTC_68|Compound:0986-0284|FD-Score:3.46|P-value:3.13E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:-3.17|P-value:7.82E-4 ID:SGTC_92|Compound:0063-0024|FD-Score:4.00|P-value:3.82E-5 ID:SGTC_94|Compound:0334-0113|FD-Score:3.22|P-value:7.36E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:-6.03|P-value:9.94E-10 ID:SGTC_103|Compound:0844-0104|FD-Score:7.32|P-value:1.97E-13 ID:SGTC_107|Compound:0358-0022|FD-Score:-3.36|P-value:3.99E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.38|P-value:4.12E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:4.18|P-value:1.77E-5 ID:SGTC_258|Compound:haloperidol|FD-Score:3.16|P-value:8.91E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:4.46|P-value:5.15E-6 ID:SGTC_384|Compound:k007-0144|FD-Score:3.83|P-value:7.49E-5 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.97|P-value:4.23E-5 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.54|P-value:2.29E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:6.64|P-value:2.43E-11 ID:SGTC_794|Compound:k038-0031|FD-Score:3.29|P-value:5.77E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:5.15|P-value:1.70E-7 ID:SGTC_855|Compound:0428-0020|FD-Score:4.02|P-value:3.53E-5 ID:SGTC_928|Compound:2764-0428|FD-Score:4.43|P-value:5.71E-6 ID:SGTC_1026|Compound:k007-1105|FD-Score:5.35|P-value:5.83E-8 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.56|P-value:2.19E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:4.80|P-value:1.03E-6 ID:SGTC_1391|Compound:3442-0225|FD-Score:3.17|P-value:8.67E-4 ID:SGTC_1886|Compound:st057356|FD-Score:3.48|P-value:2.87E-4 ID:SGTC_1904|Compound:st060225|FD-Score:5.11|P-value:2.11E-7 ID:SGTC_1919|Compound:st067113|FD-Score:-3.98|P-value:3.63E-5 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.29|P-value:5.64E-4 ID:SGTC_2028|Compound:5153890|FD-Score:3.43|P-value:3.46E-4 ID:SGTC_2126|Compound:5251174|FD-Score:-5.58|P-value:1.37E-8 ID:SGTC_2129|Compound:girgensonine|FD-Score:8.22|P-value:1.91E-16 ID:SGTC_2240|Compound:6690706|FD-Score:4.70|P-value:1.64E-6 ID:SGTC_2267|Compound:7931562|FD-Score:3.55|P-value:2.26E-4 ID:SGTC_2304|Compound:7443016|FD-Score:3.26|P-value:6.34E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.92|P-value:5.38E-5 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:5.43|P-value:3.72E-8 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:4.04|P-value:3.23E-5 ID:SGTC_2725|Compound:haloperidol|FD-Score:3.60|P-value:1.89E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:4.10|P-value:2.45E-5 ID:SGTC_2844|Compound:9010103|FD-Score:3.85|P-value:7.15E-5 ID:SGTC_2863|Compound:9028737|FD-Score:3.28|P-value:6.02E-4 ID:SGTC_2869|Compound:9037858|FD-Score:4.76|P-value:1.21E-6 ID:SGTC_2910|Compound:7948405|FD-Score:3.38|P-value:4.20E-4 ID:SGTC_2993|Compound:9054257|FD-Score:4.31|P-value:9.98E-6 ID:SGTC_3050|Compound:9093513|FD-Score:3.29|P-value:5.68E-4 ID:SGTC_3056|Compound:9094900|FD-Score:3.33|P-value:5.04E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.44|P-value:3.36E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-4.06|P-value:2.58E-5 ID:SGTC_3204|Compound:9114183|FD-Score:3.19|P-value:8.10E-4 ID:SGTC_3210|Compound:9128157|FD-Score:3.82|P-value:7.77E-5 ID:SGTC_3214|Compound:9129694|FD-Score:4.61|P-value:2.51E-6 ID:SGTC_3276|Compound:9139060|FD-Score:3.21|P-value:7.60E-4 ID:SGTC_3286|Compound:9117135|FD-Score:4.12|P-value:2.32E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:4.51|P-value:4.03E-6 ID:SGTC_68|Compound:0986-0284|FD-Score:3.46|P-value:3.13E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:-3.17|P-value:7.82E-4 ID:SGTC_92|Compound:0063-0024|FD-Score:4.00|P-value:3.82E-5 ID:SGTC_94|Compound:0334-0113|FD-Score:3.22|P-value:7.36E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:-6.03|P-value:9.94E-10 ID:SGTC_103|Compound:0844-0104|FD-Score:7.32|P-value:1.97E-13 ID:SGTC_107|Compound:0358-0022|FD-Score:-3.36|P-value:3.99E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.38|P-value:4.12E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:4.18|P-value:1.77E-5 ID:SGTC_258|Compound:haloperidol|FD-Score:3.16|P-value:8.91E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:4.46|P-value:5.15E-6 ID:SGTC_384|Compound:k007-0144|FD-Score:3.83|P-value:7.49E-5 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.97|P-value:4.23E-5 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.54|P-value:2.29E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:6.64|P-value:2.43E-11 ID:SGTC_794|Compound:k038-0031|FD-Score:3.29|P-value:5.77E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:5.15|P-value:1.70E-7 ID:SGTC_855|Compound:0428-0020|FD-Score:4.02|P-value:3.53E-5 ID:SGTC_928|Compound:2764-0428|FD-Score:4.43|P-value:5.71E-6 ID:SGTC_1026|Compound:k007-1105|FD-Score:5.35|P-value:5.83E-8 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.56|P-value:2.19E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:4.80|P-value:1.03E-6 ID:SGTC_1391|Compound:3442-0225|FD-Score:3.17|P-value:8.67E-4 ID:SGTC_1886|Compound:st057356|FD-Score:3.48|P-value:2.87E-4 ID:SGTC_1904|Compound:st060225|FD-Score:5.11|P-value:2.11E-7 ID:SGTC_1919|Compound:st067113|FD-Score:-3.98|P-value:3.63E-5 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.29|P-value:5.64E-4 ID:SGTC_2028|Compound:5153890|FD-Score:3.43|P-value:3.46E-4 ID:SGTC_2126|Compound:5251174|FD-Score:-5.58|P-value:1.37E-8 ID:SGTC_2129|Compound:girgensonine|FD-Score:8.22|P-value:1.91E-16 ID:SGTC_2240|Compound:6690706|FD-Score:4.70|P-value:1.64E-6 ID:SGTC_2267|Compound:7931562|FD-Score:3.55|P-value:2.26E-4 ID:SGTC_2304|Compound:7443016|FD-Score:3.26|P-value:6.34E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.92|P-value:5.38E-5 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:5.43|P-value:3.72E-8 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:4.04|P-value:3.23E-5 ID:SGTC_2725|Compound:haloperidol|FD-Score:3.60|P-value:1.89E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:4.10|P-value:2.45E-5 ID:SGTC_2844|Compound:9010103|FD-Score:3.85|P-value:7.15E-5 ID:SGTC_2863|Compound:9028737|FD-Score:3.28|P-value:6.02E-4 ID:SGTC_2869|Compound:9037858|FD-Score:4.76|P-value:1.21E-6 ID:SGTC_2910|Compound:7948405|FD-Score:3.38|P-value:4.20E-4 ID:SGTC_2993|Compound:9054257|FD-Score:4.31|P-value:9.98E-6 ID:SGTC_3050|Compound:9093513|FD-Score:3.29|P-value:5.68E-4 ID:SGTC_3056|Compound:9094900|FD-Score:3.33|P-value:5.04E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.44|P-value:3.36E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-4.06|P-value:2.58E-5 ID:SGTC_3204|Compound:9114183|FD-Score:3.19|P-value:8.10E-4 ID:SGTC_3210|Compound:9128157|FD-Score:3.82|P-value:7.77E-5 ID:SGTC_3214|Compound:9129694|FD-Score:4.61|P-value:2.51E-6 ID:SGTC_3276|Compound:9139060|FD-Score:3.21|P-value:7.60E-4 ID:SGTC_3286|Compound:9117135|FD-Score:4.12|P-value:2.32E-5

Top fitness defect scores for YMR258C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2129 girgensonine 8.22 121.0 µM 1.91E-16 heme biosynthesis & mitochondrial translocase 3 1.05E-16
2 SGTC_103 0844-0104 7.32 51.8 µM 1.97E-13 ERAD & cell cycle 56 1.21E-13
3 SGTC_426 0988-0037 6.64 14.6 µM 2.43E-11 ERAD & cell cycle 10 1.61E-11
4 SGTC_2540 epiandrosterone 5.43 100.0 µM 3.72E-8 24 2.77E-8
5 SGTC_1026 k007-1105 5.35 30.6 µM 5.83E-8 ERAD & cell cycle 54 4.37E-8
6 SGTC_805 3852-0218 5.15 479.0 µM 1.70E-7 ERAD & cell cycle 33 1.30E-7
7 SGTC_1904 st060225 5.11 78.0 µM 2.11E-7 8 1.61E-7
8 SGTC_1165 k072-0275 4.80 30.3 µM 1.03E-6 25 8.05E-7
9 SGTC_2869 9037858 4.76 32.5 µM 1.21E-6 5 9.51E-7
10 SGTC_2240 6690706 4.70 185.9 µM 1.64E-6 7 1.30E-6
11 SGTC_3214 9129694 4.61 49.5 µM 2.51E-6 11 2.00E-6
12 SGTC_64 000l-0018 4.51 272.1 µM 4.03E-6 ubiquinone biosynthesis & proteasome 122 3.24E-6
13 SGTC_352 k048-0007 4.46 5.1 µM 5.15E-6 ERAD & cell cycle 63 4.15E-6
14 SGTC_928 2764-0428 4.43 58.7 µM 5.71E-6 cell wall 42 4.61E-6
15 SGTC_2993 9054257 4.31 71.4 µM 9.98E-6 fatty acid desaturase (OLE1) 37 8.14E-6
16 SGTC_157 1348-1695 4.18 65.8 µM 1.77E-5 iron homeostasis 56 1.46E-5
17 SGTC_3286 9117135 4.12 37.2 µM 2.32E-5 9 1.92E-5
18 SGTC_2757 clomiphene 4.10 4.2 µM 2.45E-5 fatty acid desaturase (OLE1) 33 2.03E-5
19 SGTC_2559 tetrahydrosappanone a trimethyl ether 4.04 100.0 µM 3.23E-5 13 2.69E-5
20 SGTC_855 0428-0020 4.02 25.0 µM 3.53E-5 Golgi 24 2.94E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.294 6.28E-68 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.271 1.13E-57 YOL002C IZH2 hom Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
0.263 4.24E-54 YMR029C FAR8 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.261 3.67E-53 YGL055W OLE1 het Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
0.260 6.76E-53 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.241 1.22E-45 YIL030C SSM4 hom Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.236 1.39E-43 YDR199W_d YDR199W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity
0.229 4.54E-41 YMR052C-A_d YMR052C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.196 2.69E-30 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.194 1.18E-29 YMR052W FAR3 hom Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
0.193 2.02E-29 YMR067C UBX4 hom UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress
0.193 2.52E-29 YLR065C ENV10 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene
0.193 2.36E-29 YML014W TRM9 hom tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
0.182 1.97E-26 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.181 3.81E-26 YNL026W SAM50 het Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85