YMR259C / TRM732

Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR259C / TRM732

Click on Significant Values for Screen Details

Top fitness defect scores for YMR259C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1192 1488-0095 6.10 21.4 µM 2.03E-9 5 5.44E-10
2 SGTC_1888 azatryptophan 4.97 97.5 µM 8.24E-7 7 3.36E-7
3 SGTC_2701 st077767 4.89 14.7 µM 1.18E-6 22 4.95E-7
4 SGTC_1298 1079-1278 4.85 263.0 µM 1.43E-6 DNA damage response 6 6.05E-7
5 SGTC_497 capsazepine 4.49 133.0 µM 7.33E-6 5 3.48E-6
6 SGTC_1597 7-benzyloxygramine 4.43 47.5 µM 9.61E-6 calcium & mitochondrial duress 91 4.66E-6
7 SGTC_3043 9092303 4.41 49.5 µM 1.04E-5 7 5.08E-6
8 SGTC_1042 0350-0096 4.34 82.0 µM 1.44E-5 TSC3-RPN4 18 7.19E-6
9 SGTC_3131 9127911 4.31 49.5 µM 1.65E-5 20 8.29E-6
10 SGTC_2233 sulbentine 4.30 40.4 µM 1.68E-5 44 8.47E-6
11 SGTC_218 alverine citrate 4.26 93.8 µM 1.98E-5 fatty acid desaturase (OLE1) 25 1.01E-5
12 SGTC_113 0099-0221 4.16 2.5 µM 3.07E-5 20 1.61E-5
13 SGTC_1932 st074806 4.06 40.9 µM 4.58E-5 24 2.47E-5
14 SGTC_1903 st060211 4.05 78.3 µM 4.75E-5 27 2.57E-5
15 SGTC_1419 3993-2473 4.05 62.6 µM 4.79E-5 17 2.59E-5
16 SGTC_76 0433-0009 4.04 400.3 µM 4.93E-5 13 2.67E-5
17 SGTC_1056 3448-1962 3.96 115.0 µM 6.64E-5 amide catabolism 23 3.67E-5
18 SGTC_2397 5161689 3.92 200.0 µM 7.96E-5 39 4.46E-5
19 SGTC_2831 7999036 3.87 19.5 µM 9.61E-5 51 5.46E-5
20 SGTC_961 1252-1571 3.85 372.0 µM 1.03E-4 31 5.87E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.116 1.38E-11 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.100 6.39E-9 YGL226W MTC3 hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1
0.100 7.35E-9 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.095 3.47E-8 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.094 4.46E-8 YMR182W-A_p YMR182W-A_p hom Putative protein of unknown function
0.092 8.12E-8 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.089 2.51E-7 YDR321W ASP1 hom Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively
0.086 6.61E-7 YDL186W_p YDL186W_p hom Putative protein of unknown function; YDL186W is not an essential gene
0.083 1.43E-6 YMR129W POM152 hom Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210
0.081 2.76E-6 YNR032C-A HUB1 hom Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
0.080 3.22E-6 YHR147C MRPL6 hom Mitochondrial ribosomal protein of the large subunit
0.080 3.25E-6 YJL149W DAS1 hom Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C
0.078 5.74E-6 YBR085W AAC3 hom Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication
0.074 1.91E-5 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.073 2.22E-5 YML084W_d YML084W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data