YMR275C / BUL1

Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR275C / BUL1

Click on Significant Values for Screen Details ID:SGTC_114|Compound:0364-0001|FD-Score:-4.46|P-value:9.93E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:-4.29|P-value:2.02E-5 ID:SGTC_123|Compound:0083-0120|FD-Score:5.25|P-value:2.95E-7 ID:SGTC_126|Compound:0417-1624|FD-Score:3.79|P-value:1.59E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.64|P-value:5.08E-6 ID:SGTC_210|Compound:3551-0445|FD-Score:4.25|P-value:2.67E-5 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.66|P-value:2.55E-4 ID:SGTC_263|Compound:alizarin|FD-Score:4.10|P-value:4.91E-5 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:3.86|P-value:1.22E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:4.56|P-value:7.14E-6 ID:SGTC_367|Compound:0335-0881|FD-Score:-4.18|P-value:3.23E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:3.38|P-value:6.56E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:3.57|P-value:3.41E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:11.10|P-value:2.92E-26 ID:SGTC_863|Compound:0466-0191|FD-Score:4.54|P-value:7.83E-6 ID:SGTC_871|Compound:0574-0004|FD-Score:4.07|P-value:5.45E-5 ID:SGTC_879|Compound:0831-1030|FD-Score:4.93|P-value:1.36E-6 ID:SGTC_964|Compound:1287-0107|FD-Score:4.14|P-value:4.14E-5 ID:SGTC_975|Compound:1335-0046|FD-Score:3.45|P-value:5.26E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:-3.31|P-value:7.56E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.57|P-value:3.42E-4 ID:SGTC_1307|Compound:1187-1599|FD-Score:3.46|P-value:4.95E-4 ID:SGTC_1363|Compound:1636-0063|FD-Score:4.98|P-value:1.09E-6 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:4.35|P-value:1.76E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.91|P-value:9.02E-5 ID:SGTC_1752|Compound:st044819|FD-Score:3.30|P-value:8.67E-4 ID:SGTC_1759|Compound:st043059|FD-Score:3.57|P-value:3.47E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.40|P-value:1.43E-5 ID:SGTC_1848|Compound:st056003|FD-Score:3.64|P-value:2.70E-4 ID:SGTC_1923|Compound:st058458|FD-Score:4.07|P-value:5.40E-5 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:3.44|P-value:5.44E-4 ID:SGTC_2092|Compound:5303694|FD-Score:4.17|P-value:3.64E-5 ID:SGTC_2231|Compound:micatex|FD-Score:-3.72|P-value:1.88E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:4.88|P-value:1.75E-6 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-4.11|P-value:4.23E-5 ID:SGTC_2388|Compound:9080168|FD-Score:3.35|P-value:7.18E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:4.26|P-value:2.51E-5 ID:SGTC_2461|Compound:5755302|FD-Score:3.31|P-value:8.17E-4 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:3.80|P-value:1.50E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-3.47|P-value:4.47E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:-3.46|P-value:4.59E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:6.57|P-value:2.01E-10 ID:SGTC_2619|Compound:obliquin|FD-Score:3.96|P-value:8.16E-5 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:6.93|P-value:2.04E-11 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:6.30|P-value:1.02E-9 ID:SGTC_2638|Compound:epoxomicin|FD-Score:3.77|P-value:1.71E-4 ID:SGTC_2747|Compound:doxorubicin|FD-Score:3.36|P-value:7.07E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:-4.81|P-value:2.09E-6 ID:SGTC_2833|Compound:9001315|FD-Score:-3.77|P-value:1.55E-4 ID:SGTC_2884|Compound:9058502|FD-Score:4.43|P-value:1.27E-5 ID:SGTC_2912|Compound:7964298|FD-Score:3.59|P-value:3.20E-4 ID:SGTC_2944|Compound:9067346|FD-Score:-4.41|P-value:1.24E-5 ID:SGTC_2948|Compound:9070189|FD-Score:5.36|P-value:1.71E-7 ID:SGTC_2950|Compound:9072895|FD-Score:-4.34|P-value:1.67E-5 ID:SGTC_2991|Compound:9074669|FD-Score:-3.87|P-value:1.07E-4 ID:SGTC_3044|Compound:9093193|FD-Score:3.93|P-value:9.28E-5 ID:SGTC_3046|Compound:9092212|FD-Score:3.47|P-value:4.78E-4 ID:SGTC_3114|Compound:9122766|FD-Score:3.97|P-value:8.12E-5 ID:SGTC_3148|Compound:9097893|FD-Score:5.24|P-value:3.04E-7 ID:SGTC_3210|Compound:9128157|FD-Score:3.34|P-value:7.61E-4 ID:SGTC_3266|Compound:9136860|FD-Score:3.70|P-value:2.15E-4 ID:SGTC_3311|Compound:9131146|FD-Score:7.70|P-value:1.13E-13 ID:SGTC_3321|Compound:9137793|FD-Score:4.10|P-value:4.92E-5 ID:SGTC_3350|Compound:9150499|FD-Score:3.55|P-value:3.70E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:-4.46|P-value:9.93E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:-4.29|P-value:2.02E-5 ID:SGTC_123|Compound:0083-0120|FD-Score:5.25|P-value:2.95E-7 ID:SGTC_126|Compound:0417-1624|FD-Score:3.79|P-value:1.59E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:4.64|P-value:5.08E-6 ID:SGTC_210|Compound:3551-0445|FD-Score:4.25|P-value:2.67E-5 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.66|P-value:2.55E-4 ID:SGTC_263|Compound:alizarin|FD-Score:4.10|P-value:4.91E-5 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:3.86|P-value:1.22E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:4.56|P-value:7.14E-6 ID:SGTC_367|Compound:0335-0881|FD-Score:-4.18|P-value:3.23E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:3.38|P-value:6.56E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:3.57|P-value:3.41E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:11.10|P-value:2.92E-26 ID:SGTC_863|Compound:0466-0191|FD-Score:4.54|P-value:7.83E-6 ID:SGTC_871|Compound:0574-0004|FD-Score:4.07|P-value:5.45E-5 ID:SGTC_879|Compound:0831-1030|FD-Score:4.93|P-value:1.36E-6 ID:SGTC_964|Compound:1287-0107|FD-Score:4.14|P-value:4.14E-5 ID:SGTC_975|Compound:1335-0046|FD-Score:3.45|P-value:5.26E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:-3.31|P-value:7.56E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.57|P-value:3.42E-4 ID:SGTC_1307|Compound:1187-1599|FD-Score:3.46|P-value:4.95E-4 ID:SGTC_1363|Compound:1636-0063|FD-Score:4.98|P-value:1.09E-6 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:4.35|P-value:1.76E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.91|P-value:9.02E-5 ID:SGTC_1752|Compound:st044819|FD-Score:3.30|P-value:8.67E-4 ID:SGTC_1759|Compound:st043059|FD-Score:3.57|P-value:3.47E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.40|P-value:1.43E-5 ID:SGTC_1848|Compound:st056003|FD-Score:3.64|P-value:2.70E-4 ID:SGTC_1923|Compound:st058458|FD-Score:4.07|P-value:5.40E-5 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:3.44|P-value:5.44E-4 ID:SGTC_2092|Compound:5303694|FD-Score:4.17|P-value:3.64E-5 ID:SGTC_2231|Compound:micatex|FD-Score:-3.72|P-value:1.88E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:4.88|P-value:1.75E-6 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-4.11|P-value:4.23E-5 ID:SGTC_2388|Compound:9080168|FD-Score:3.35|P-value:7.18E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:4.26|P-value:2.51E-5 ID:SGTC_2461|Compound:5755302|FD-Score:3.31|P-value:8.17E-4 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:3.80|P-value:1.50E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-3.47|P-value:4.47E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:-3.46|P-value:4.59E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:6.57|P-value:2.01E-10 ID:SGTC_2619|Compound:obliquin|FD-Score:3.96|P-value:8.16E-5 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:6.93|P-value:2.04E-11 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:6.30|P-value:1.02E-9 ID:SGTC_2638|Compound:epoxomicin|FD-Score:3.77|P-value:1.71E-4 ID:SGTC_2747|Compound:doxorubicin|FD-Score:3.36|P-value:7.07E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:-4.81|P-value:2.09E-6 ID:SGTC_2833|Compound:9001315|FD-Score:-3.77|P-value:1.55E-4 ID:SGTC_2884|Compound:9058502|FD-Score:4.43|P-value:1.27E-5 ID:SGTC_2912|Compound:7964298|FD-Score:3.59|P-value:3.20E-4 ID:SGTC_2944|Compound:9067346|FD-Score:-4.41|P-value:1.24E-5 ID:SGTC_2948|Compound:9070189|FD-Score:5.36|P-value:1.71E-7 ID:SGTC_2950|Compound:9072895|FD-Score:-4.34|P-value:1.67E-5 ID:SGTC_2991|Compound:9074669|FD-Score:-3.87|P-value:1.07E-4 ID:SGTC_3044|Compound:9093193|FD-Score:3.93|P-value:9.28E-5 ID:SGTC_3046|Compound:9092212|FD-Score:3.47|P-value:4.78E-4 ID:SGTC_3114|Compound:9122766|FD-Score:3.97|P-value:8.12E-5 ID:SGTC_3148|Compound:9097893|FD-Score:5.24|P-value:3.04E-7 ID:SGTC_3210|Compound:9128157|FD-Score:3.34|P-value:7.61E-4 ID:SGTC_3266|Compound:9136860|FD-Score:3.70|P-value:2.15E-4 ID:SGTC_3311|Compound:9131146|FD-Score:7.70|P-value:1.13E-13 ID:SGTC_3321|Compound:9137793|FD-Score:4.10|P-value:4.92E-5 ID:SGTC_3350|Compound:9150499|FD-Score:3.55|P-value:3.70E-4

Top fitness defect scores for YMR275C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_690 glyoxal 11.10 20.3 µM 2.92E-26 15 9.92E-29
2 SGTC_3311 9131146 7.70 2.9 µM 1.13E-13 cell wall 36 6.86E-15
3 SGTC_2635 hydroquinidine 6.93 100.0 µM 2.04E-11 1 2.07E-12
4 SGTC_2584 androsterone 6.57 48.7 µM 2.01E-10 cell wall 44 2.54E-11
5 SGTC_2637 3-alpha-acetoxydihydrodeoxygedunin 6.30 100.0 µM 1.02E-9 2 1.51E-10
6 SGTC_2948 9070189 5.36 9.4 µM 1.71E-7 plasma membrane duress 28 4.22E-8
7 SGTC_123 0083-0120 5.25 66.7 µM 2.95E-7 25 7.68E-8
8 SGTC_3148 9097893 5.24 49.5 µM 3.04E-7 19 7.92E-8
9 SGTC_1363 1636-0063 4.98 10.4 µM 1.09E-6 Golgi 10 3.24E-7
10 SGTC_879 0831-1030 4.93 162.0 µM 1.36E-6 24 4.11E-7
11 SGTC_2233 sulbentine 4.88 40.4 µM 1.75E-6 37 5.44E-7
12 SGTC_138 0850-0259 4.64 40.9 µM 5.08E-6 cell wall 160 1.75E-6
13 SGTC_304 1310-0298 4.56 97.0 µM 7.14E-6 cell wall 38 2.54E-6
14 SGTC_863 0466-0191 4.54 129.0 µM 7.83E-6 cell wall 53 2.81E-6
15 SGTC_2884 9058502 4.43 52.0 µM 1.27E-5 18 4.75E-6
16 SGTC_1844 rapamycin 4.40 4.0 nM 1.43E-5 133 5.43E-6
17 SGTC_1420 4'-methoxyflavone 4.35 42.0 µM 1.76E-5 8 6.83E-6
18 SGTC_2410 aloe-emodin-8-o-glycoside 4.26 46.3 µM 2.51E-5 mitochondrial processes 23 1.01E-5
19 SGTC_210 3551-0445 4.25 56.8 µM 2.67E-5 56 1.08E-5
20 SGTC_2092 5303694 4.17 80.7 µM 3.64E-5 TSC3-RPN4 37 1.51E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.318 1.17E-79 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.287 1.66E-64 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.264 1.85E-54 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.261 3.12E-53 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.255 4.96E-51 YPL115C BEM3 hom Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.244 1.24E-46 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.236 7.19E-44 YJL073W JEM1 hom DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2
0.212 1.77E-35 YML117W NAB6 hom Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
0.209 1.37E-34 YGR163W GTR2 hom Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.205 3.17E-33 YBL062W_d YBL062W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.205 4.39E-33 YPR165W RHO1 het GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
0.204 5.52E-33 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.204 6.07E-33 YNL229C URE2 hom Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
0.203 1.09E-32 YNR040W_p YNR040W_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.200 1.02E-31 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2