YMR276W / DSK2

Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR276W / DSK2

Click on Significant Values for Screen Details ID:SGTC_38|Compound:0929-0051|FD-Score:4.01|P-value:4.84E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:11.60|P-value:1.80E-28 ID:SGTC_145|Compound:3737-0113|FD-Score:4.08|P-value:3.70E-5 ID:SGTC_163|Compound:1327-0036|FD-Score:3.65|P-value:1.86E-4 ID:SGTC_357|Compound:1325-0123|FD-Score:3.25|P-value:7.36E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:4.01|P-value:4.93E-5 ID:SGTC_517|Compound:1365-0151|FD-Score:-4.02|P-value:1.27E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:3.82|P-value:9.92E-5 ID:SGTC_728|Compound:3030-5151|FD-Score:3.41|P-value:4.28E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:-3.52|P-value:7.20E-4 ID:SGTC_821|Compound:1114-0266|FD-Score:-3.50|P-value:7.64E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-3.93|P-value:1.77E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:3.35|P-value:5.22E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:5.55|P-value:4.42E-8 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.39|P-value:4.65E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:4.00|P-value:4.96E-5 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:3.70|P-value:1.56E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.50|P-value:3.18E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:3.65|P-value:1.83E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:4.56|P-value:5.07E-6 ID:SGTC_1390|Compound:3413-2614|FD-Score:4.00|P-value:4.95E-5 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:3.38|P-value:4.80E-4 ID:SGTC_1467|Compound:k284-1082|FD-Score:3.17|P-value:9.53E-4 ID:SGTC_1646|Compound:st011709|FD-Score:4.03|P-value:4.48E-5 ID:SGTC_1676|Compound:st018489|FD-Score:4.11|P-value:3.26E-5 ID:SGTC_1681|Compound:tripelennamine|FD-Score:3.47|P-value:3.55E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.22|P-value:7.92E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.56|P-value:2.56E-4 ID:SGTC_2184|Compound:6075246|FD-Score:4.09|P-value:3.47E-5 ID:SGTC_2245|Compound:6637752|FD-Score:-4.65|P-value:1.04E-5 ID:SGTC_2251|Compound:lansoprazole|FD-Score:-4.28|P-value:4.73E-5 ID:SGTC_2443|Compound:5493264|FD-Score:3.43|P-value:3.97E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:4.02|P-value:4.60E-5 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-4.71|P-value:8.17E-6 ID:SGTC_2593|Compound:nobiletin|FD-Score:-3.82|P-value:2.58E-4 ID:SGTC_2773|Compound:thioridazine|FD-Score:3.97|P-value:5.58E-5 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:4.17|P-value:2.59E-5 ID:SGTC_2856|Compound:9022925|FD-Score:3.63|P-value:1.97E-4 ID:SGTC_2882|Compound:9058044|FD-Score:3.97|P-value:5.66E-5 ID:SGTC_2941|Compound:9056145|FD-Score:-4.01|P-value:1.32E-4 ID:SGTC_2946|Compound:9065502|FD-Score:3.64|P-value:1.92E-4 ID:SGTC_2949|Compound:9072542|FD-Score:4.11|P-value:3.30E-5 ID:SGTC_3009|Compound:9080819|FD-Score:3.23|P-value:7.72E-4 ID:SGTC_3202|Compound:9113761|FD-Score:3.46|P-value:3.65E-4 ID:SGTC_38|Compound:0929-0051|FD-Score:4.01|P-value:4.84E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:11.60|P-value:1.80E-28 ID:SGTC_145|Compound:3737-0113|FD-Score:4.08|P-value:3.70E-5 ID:SGTC_163|Compound:1327-0036|FD-Score:3.65|P-value:1.86E-4 ID:SGTC_357|Compound:1325-0123|FD-Score:3.25|P-value:7.36E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:4.01|P-value:4.93E-5 ID:SGTC_517|Compound:1365-0151|FD-Score:-4.02|P-value:1.27E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:3.82|P-value:9.92E-5 ID:SGTC_728|Compound:3030-5151|FD-Score:3.41|P-value:4.28E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:-3.52|P-value:7.20E-4 ID:SGTC_821|Compound:1114-0266|FD-Score:-3.50|P-value:7.64E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-3.93|P-value:1.77E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:3.35|P-value:5.22E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:5.55|P-value:4.42E-8 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.39|P-value:4.65E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:4.00|P-value:4.96E-5 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:3.70|P-value:1.56E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.50|P-value:3.18E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:3.65|P-value:1.83E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:4.56|P-value:5.07E-6 ID:SGTC_1390|Compound:3413-2614|FD-Score:4.00|P-value:4.95E-5 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:3.38|P-value:4.80E-4 ID:SGTC_1467|Compound:k284-1082|FD-Score:3.17|P-value:9.53E-4 ID:SGTC_1646|Compound:st011709|FD-Score:4.03|P-value:4.48E-5 ID:SGTC_1676|Compound:st018489|FD-Score:4.11|P-value:3.26E-5 ID:SGTC_1681|Compound:tripelennamine|FD-Score:3.47|P-value:3.55E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.22|P-value:7.92E-4 ID:SGTC_2131|Compound:toluylene red|FD-Score:3.56|P-value:2.56E-4 ID:SGTC_2184|Compound:6075246|FD-Score:4.09|P-value:3.47E-5 ID:SGTC_2245|Compound:6637752|FD-Score:-4.65|P-value:1.04E-5 ID:SGTC_2251|Compound:lansoprazole|FD-Score:-4.28|P-value:4.73E-5 ID:SGTC_2443|Compound:5493264|FD-Score:3.43|P-value:3.97E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:4.02|P-value:4.60E-5 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-4.71|P-value:8.17E-6 ID:SGTC_2593|Compound:nobiletin|FD-Score:-3.82|P-value:2.58E-4 ID:SGTC_2773|Compound:thioridazine|FD-Score:3.97|P-value:5.58E-5 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:4.17|P-value:2.59E-5 ID:SGTC_2856|Compound:9022925|FD-Score:3.63|P-value:1.97E-4 ID:SGTC_2882|Compound:9058044|FD-Score:3.97|P-value:5.66E-5 ID:SGTC_2941|Compound:9056145|FD-Score:-4.01|P-value:1.32E-4 ID:SGTC_2946|Compound:9065502|FD-Score:3.64|P-value:1.92E-4 ID:SGTC_2949|Compound:9072542|FD-Score:4.11|P-value:3.30E-5 ID:SGTC_3009|Compound:9080819|FD-Score:3.23|P-value:7.72E-4 ID:SGTC_3202|Compound:9113761|FD-Score:3.46|P-value:3.65E-4

Top fitness defect scores for YMR276W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_103 0844-0104 11.60 51.8 µM 1.80E-28 ERAD & cell cycle 22 3.33E-31
2 SGTC_1016 4226-1401 5.55 38.0 µM 4.42E-8 RNA pol III & RNase P/MRP 4 1.42E-8
3 SGTC_1284 0929-0043 4.56 16.0 µM 5.07E-6 14 2.59E-6
4 SGTC_2775 cyproheptadine 4.17 69.6 µM 2.59E-5 15 1.54E-5
5 SGTC_1676 st018489 4.11 23.4 µM 3.26E-5 60S ribosome export 30 1.98E-5
6 SGTC_2949 9072542 4.11 2.2 µM 3.30E-5 TSC3-RPN4 33 2.00E-5
7 SGTC_2184 6075246 4.09 200.0 µM 3.47E-5 20 2.11E-5
8 SGTC_145 3737-0113 4.08 38.4 µM 3.70E-5 DNA intercalators 69 2.27E-5
9 SGTC_1646 st011709 4.03 75.6 µM 4.48E-5 6 2.79E-5
10 SGTC_2513 baicalein 4.02 25.6 µM 4.60E-5 calcium & mitochondrial duress 26 2.88E-5
11 SGTC_38 0929-0051 4.01 58.3 µM 4.84E-5 15 3.04E-5
12 SGTC_513 4245-1575 4.01 41.7 µM 4.93E-5 unfolded protein response 113 3.10E-5
13 SGTC_1390 3413-2614 4.00 145.0 µM 4.95E-5 fatty acid desaturase (OLE1) 26 3.11E-5
14 SGTC_1134 3477-0009 4.00 42.5 µM 4.96E-5 43 3.12E-5
15 SGTC_2773 thioridazine 3.97 9.1 µM 5.58E-5 8 3.55E-5
16 SGTC_2882 9058044 3.97 58.4 µM 5.66E-5 16 3.60E-5
17 SGTC_568 1193-0097 3.82 7.5 µM 9.92E-5 ERAD & cell cycle 161 6.64E-5
18 SGTC_1135 dihydrocapsaicin 3.70 536.7 nM 1.56E-4 44 1.09E-4
19 SGTC_1271 0832-2808 3.65 169.0 µM 1.83E-4 20 1.29E-4
20 SGTC_163 1327-0036 3.65 7.5 µM 1.86E-4 127 1.32E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.332 2.89E-87 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.256 2.66E-51 YOL065C INP54 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
0.222 9.92E-39 YDL126C CDC48 het AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP
0.210 8.43E-35 YJR117W STE24 hom Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
0.208 4.34E-34 YOR363C PIP2 hom Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication
0.194 7.49E-30 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.192 4.47E-29 YMR029C FAR8 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.189 2.60E-28 YER041W YEN1 hom Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p
0.187 7.36E-28 YOL002C IZH2 hom Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
0.187 1.00E-27 YDL122W UBP1 hom Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains
0.177 5.01E-25 YNL179C_d YNL179C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance
0.175 1.49E-24 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.173 6.11E-24 YAL051W OAF1 hom Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication
0.171 2.16E-23 YPL210C SRP72 het Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane
0.169 6.57E-23 YDR199W_d YDR199W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity