HIPHOP chemogenomics database
YMR281W / GPI12 
ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp
Zygosity: Heterozygous strain
fixedexpanded
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Top fitness defect scores for YMR281W deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Cofit Genes
Download Cofitness data (tab-delimited text) (excel)| Correlation | pval | ORF | Gene | Zygosity | Description |
|---|---|---|---|---|---|
| 0.145 | 3.54E-17 | YJL203W | PRP21 | het | Subunit of the SF3a splicing factor complex, required for spliceosome assembly |
| 0.124 | 5.63E-13 | YNL142W | MEP2 | hom | Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation |
| 0.109 | 2.35E-10 | YNL262W | POL2 | het | Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p |
| 0.101 | 4.27E-9 | YOR371C | GPB1 | hom | Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication |
| 0.096 | 2.35E-8 | YJL078C | PRY3 | hom | Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p |
| 0.091 | 1.18E-7 | YJL067W_d | YJL067W_d | hom | Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data |
| 0.081 | 2.51E-6 | YBR212W | NGR1 | hom | RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase |
| 0.078 | 5.54E-6 | YBR040W | FIG1 | hom | Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating |
| 0.078 | 6.65E-6 | YOL141W | PPM2 | hom | AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p |
| 0.076 | 9.30E-6 | YPR201W | ARR3 | hom | Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite |
| 0.076 | 9.68E-6 | YDR043C | NRG1 | hom | Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; NRG1 has a paralog, NRG2, that arose from the whole genome duplication |
| 0.076 | 1.08E-5 | YBR084C-A | RPL19A | hom | Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication |
| 0.076 | 1.18E-5 | YML083C | YML083C | hom | Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions |
| 0.075 | 1.42E-5 | YBL043W | ECM13 | hom | Non-essential protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; ECM13 has a paralog, YJR115W, that arose from the whole genome duplication |
| 0.074 | 2.01E-5 | YNL290W | RFC3 | het | Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon |