YMR281W / GPI12

ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp

Zygosity: Heterozygous strain
fixedexpanded
Profile for YMR281W / GPI12

Click on Significant Values for Screen Details ID:SGTC_114|Compound:0364-0001|FD-Score:3.09|P-value:6.04E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:3.24|P-value:3.33E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.12|P-value:5.42E-4 ID:SGTC_337|Compound:1315-0038|FD-Score:4.57|P-value:8.11E-7 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-4.62|P-value:5.81E-7 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-3.70|P-value:5.01E-5 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:3.04|P-value:7.19E-4 ID:SGTC_417|Compound:st075190|FD-Score:3.30|P-value:2.66E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:3.42|P-value:1.65E-4 ID:SGTC_565|Compound:1418-0001|FD-Score:9.07|P-value:8.27E-22 ID:SGTC_570|Compound:0987-0065|FD-Score:3.24|P-value:3.37E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:-5.00|P-value:7.12E-8 ID:SGTC_594|Compound:1487-1584|FD-Score:4.23|P-value:4.45E-6 ID:SGTC_597|Compound:k018-0003|FD-Score:-3.01|P-value:7.82E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:3.46|P-value:1.41E-4 ID:SGTC_685|Compound:1278-0033|FD-Score:3.37|P-value:2.06E-4 ID:SGTC_697|Compound:0508-1955|FD-Score:4.64|P-value:5.71E-7 ID:SGTC_702|Compound:1488-0190|FD-Score:4.13|P-value:7.27E-6 ID:SGTC_826|Compound:1683-5028|FD-Score:4.01|P-value:1.28E-5 ID:SGTC_865|Compound:0488-0499|FD-Score:3.07|P-value:6.48E-4 ID:SGTC_870|Compound:0573-2213|FD-Score:-4.56|P-value:8.32E-7 ID:SGTC_874|Compound:0599-0792|FD-Score:3.05|P-value:6.77E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:3.14|P-value:4.99E-4 ID:SGTC_1216|Compound:1165-0504|FD-Score:3.06|P-value:6.68E-4 ID:SGTC_1219|Compound:1469-0211|FD-Score:4.48|P-value:1.30E-6 ID:SGTC_1229|Compound:0139-0193|FD-Score:-3.18|P-value:4.06E-4 ID:SGTC_1236|Compound:0330-0013|FD-Score:3.79|P-value:3.50E-5 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.44|P-value:1.51E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:3.61|P-value:7.59E-5 ID:SGTC_1458|Compound:k015-0034|FD-Score:3.34|P-value:2.33E-4 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:4.50|P-value:1.15E-6 ID:SGTC_1750|Compound:st081859|FD-Score:3.04|P-value:7.22E-4 ID:SGTC_1766|Compound:st045414|FD-Score:3.03|P-value:7.33E-4 ID:SGTC_1772|Compound:st048439|FD-Score:-3.21|P-value:3.69E-4 ID:SGTC_1794|Compound:st048918|FD-Score:3.11|P-value:5.49E-4 ID:SGTC_1941|Compound:st076221|FD-Score:4.40|P-value:1.96E-6 ID:SGTC_2271|Compound:7849410|FD-Score:-3.20|P-value:3.80E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:3.66|P-value:6.19E-5 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-3.09|P-value:5.85E-4 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.49|P-value:1.26E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:5.37|P-value:8.45E-9 ID:SGTC_2794|Compound:7785382|FD-Score:4.23|P-value:4.49E-6 ID:SGTC_2804|Compound:7954240|FD-Score:6.37|P-value:1.14E-11 ID:SGTC_2810|Compound:7959871|FD-Score:4.21|P-value:5.03E-6 ID:SGTC_2874|Compound:9043904|FD-Score:2.97|P-value:9.33E-4 ID:SGTC_2907|Compound:7721985|FD-Score:-3.35|P-value:2.13E-4 ID:SGTC_3028|Compound:9087778|FD-Score:-3.44|P-value:1.50E-4 ID:SGTC_3068|Compound:9116768|FD-Score:3.19|P-value:4.04E-4 ID:SGTC_3123|Compound:9126162|FD-Score:-3.40|P-value:1.77E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-3.05|P-value:6.59E-4 ID:SGTC_3208|Compound:9114115|FD-Score:4.95|P-value:1.03E-7 ID:SGTC_3348|Compound:9149464|FD-Score:-3.07|P-value:6.11E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:3.09|P-value:6.04E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:3.24|P-value:3.33E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.12|P-value:5.42E-4 ID:SGTC_337|Compound:1315-0038|FD-Score:4.57|P-value:8.11E-7 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-4.62|P-value:5.81E-7 ID:SGTC_399|Compound:oxolinic acid|FD-Score:-3.70|P-value:5.01E-5 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:3.04|P-value:7.19E-4 ID:SGTC_417|Compound:st075190|FD-Score:3.30|P-value:2.66E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:3.42|P-value:1.65E-4 ID:SGTC_565|Compound:1418-0001|FD-Score:9.07|P-value:8.27E-22 ID:SGTC_570|Compound:0987-0065|FD-Score:3.24|P-value:3.37E-4 ID:SGTC_573|Compound:3702-0553|FD-Score:-5.00|P-value:7.12E-8 ID:SGTC_594|Compound:1487-1584|FD-Score:4.23|P-value:4.45E-6 ID:SGTC_597|Compound:k018-0003|FD-Score:-3.01|P-value:7.82E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:3.46|P-value:1.41E-4 ID:SGTC_685|Compound:1278-0033|FD-Score:3.37|P-value:2.06E-4 ID:SGTC_697|Compound:0508-1955|FD-Score:4.64|P-value:5.71E-7 ID:SGTC_702|Compound:1488-0190|FD-Score:4.13|P-value:7.27E-6 ID:SGTC_826|Compound:1683-5028|FD-Score:4.01|P-value:1.28E-5 ID:SGTC_865|Compound:0488-0499|FD-Score:3.07|P-value:6.48E-4 ID:SGTC_870|Compound:0573-2213|FD-Score:-4.56|P-value:8.32E-7 ID:SGTC_874|Compound:0599-0792|FD-Score:3.05|P-value:6.77E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:3.14|P-value:4.99E-4 ID:SGTC_1216|Compound:1165-0504|FD-Score:3.06|P-value:6.68E-4 ID:SGTC_1219|Compound:1469-0211|FD-Score:4.48|P-value:1.30E-6 ID:SGTC_1229|Compound:0139-0193|FD-Score:-3.18|P-value:4.06E-4 ID:SGTC_1236|Compound:0330-0013|FD-Score:3.79|P-value:3.50E-5 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.44|P-value:1.51E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:3.61|P-value:7.59E-5 ID:SGTC_1458|Compound:k015-0034|FD-Score:3.34|P-value:2.33E-4 ID:SGTC_1599|Compound:8-chlro-1-tetrahydronorharmanone|FD-Score:4.50|P-value:1.15E-6 ID:SGTC_1750|Compound:st081859|FD-Score:3.04|P-value:7.22E-4 ID:SGTC_1766|Compound:st045414|FD-Score:3.03|P-value:7.33E-4 ID:SGTC_1772|Compound:st048439|FD-Score:-3.21|P-value:3.69E-4 ID:SGTC_1794|Compound:st048918|FD-Score:3.11|P-value:5.49E-4 ID:SGTC_1941|Compound:st076221|FD-Score:4.40|P-value:1.96E-6 ID:SGTC_2271|Compound:7849410|FD-Score:-3.20|P-value:3.80E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:3.66|P-value:6.19E-5 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:-3.09|P-value:5.85E-4 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.49|P-value:1.26E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:5.37|P-value:8.45E-9 ID:SGTC_2794|Compound:7785382|FD-Score:4.23|P-value:4.49E-6 ID:SGTC_2804|Compound:7954240|FD-Score:6.37|P-value:1.14E-11 ID:SGTC_2810|Compound:7959871|FD-Score:4.21|P-value:5.03E-6 ID:SGTC_2874|Compound:9043904|FD-Score:2.97|P-value:9.33E-4 ID:SGTC_2907|Compound:7721985|FD-Score:-3.35|P-value:2.13E-4 ID:SGTC_3028|Compound:9087778|FD-Score:-3.44|P-value:1.50E-4 ID:SGTC_3068|Compound:9116768|FD-Score:3.19|P-value:4.04E-4 ID:SGTC_3123|Compound:9126162|FD-Score:-3.40|P-value:1.77E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-3.05|P-value:6.59E-4 ID:SGTC_3208|Compound:9114115|FD-Score:4.95|P-value:1.03E-7 ID:SGTC_3348|Compound:9149464|FD-Score:-3.07|P-value:6.11E-4

Top fitness defect scores for YMR281W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_565 1418-0001 9.07 44.5 µM 8.27E-22 1 5.90E-20 3.55
2 SGTC_2804 7954240 6.37 19.5 µM 1.14E-11 3 9.48E-11 0.09
3 SGTC_2765 myclobutanil 5.37 7.7 µM 8.45E-9 azole & statin 2 3.85E-8 0.47
4 SGTC_3208 9114115 4.95 49.5 µM 1.03E-7 3 3.74E-7 0.27
5 SGTC_697 0508-1955 4.64 564.0 µM 5.71E-7 2 1.78E-6 0.92
6 SGTC_337 1315-0038 4.57 354.0 µM 8.11E-7 amide catabolism 2 2.45E-6 0.26
7 SGTC_1599 8-chlro-1-tetrahydronorharmanone 4.50 90.6 µM 1.15E-6 7 3.35E-6 0.52
8 SGTC_1219 1469-0211 4.48 33.1 µM 1.30E-6 2 3.77E-6 0.18
9 SGTC_1941 st076221 4.40 61.8 µM 1.96E-6 sphingolipid biosynthesis & PDR1 8 5.45E-6 0.48
10 SGTC_594 1487-1584 4.23 10.8 µM 4.45E-6 7 1.15E-5 0.33
11 SGTC_2794 7785382 4.23 27.3 µM 4.49E-6 11 1.16E-5 0.05
12 SGTC_2810 7959871 4.21 45.5 µM 5.03E-6 copper-dependent oxidative stress 15 1.29E-5 0.12
13 SGTC_702 1488-0190 4.13 94.0 µM 7.27E-6 amide catabolism 1 1.80E-5 0.54
14 SGTC_826 1683-5028 4.01 118.0 µM 1.28E-5 4 3.01E-5 0.54
15 SGTC_1236 0330-0013 3.79 258.0 µM 3.50E-5 3 7.55E-5 0.53
16 SGTC_2544 sphondin 3.66 68.5 µM 6.19E-5 RNA pol III & RNase P/MRP 42 1.27E-4 0.10
17 SGTC_1331 1472-0049 3.61 76.2 µM 7.59E-5 9 1.53E-4 0.27
18 SGTC_2764 methyldopa 3.49 94.7 µM 1.26E-4 7 2.42E-4 0.11
19 SGTC_604 1000-0114 3.46 314.0 µM 1.41E-4 5 2.70E-4 0.28
20 SGTC_1281 0884-0031 3.44 11.8 µM 1.51E-4 fatty acid desaturase (OLE1) 6 2.87E-4 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.145 3.54E-17 YJL203W PRP21 het Subunit of the SF3a splicing factor complex, required for spliceosome assembly
0.124 5.63E-13 YNL142W MEP2 hom Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation
0.109 2.35E-10 YNL262W POL2 het Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
0.101 4.27E-9 YOR371C GPB1 hom Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication
0.096 2.35E-8 YJL078C PRY3 hom Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p
0.091 1.18E-7 YJL067W_d YJL067W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.081 2.51E-6 YBR212W NGR1 hom RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase
0.078 5.54E-6 YBR040W FIG1 hom Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating
0.078 6.65E-6 YOL141W PPM2 hom AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p
0.076 9.30E-6 YPR201W ARR3 hom Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite
0.076 9.68E-6 YDR043C NRG1 hom Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; NRG1 has a paralog, NRG2, that arose from the whole genome duplication
0.076 1.08E-5 YBR084C-A RPL19A hom Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication
0.076 1.18E-5 YML083C YML083C hom Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions
0.075 1.42E-5 YBL043W ECM13 hom Non-essential protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; ECM13 has a paralog, YJR115W, that arose from the whole genome duplication
0.074 2.01E-5 YNL290W RFC3 het Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon