YMR288W / HSH155

U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155

Zygosity: Heterozygous strain
fixedexpanded
Profile for YMR288W / HSH155

Click on Significant Values for Screen Details ID:SGTC_41|Compound:0478-0632|FD-Score:3.88|P-value:9.94E-6 ID:SGTC_234|Compound:nsc-17383|FD-Score:2.84|P-value:8.80E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.09|P-value:3.30E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:2.84|P-value:8.81E-4 ID:SGTC_437|Compound:s-farnesyl-l-cysteine methyl ester|FD-Score:-2.94|P-value:8.21E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:3.39|P-value:9.81E-5 ID:SGTC_592|Compound:k241-0073|FD-Score:3.13|P-value:2.79E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:3.74|P-value:2.01E-5 ID:SGTC_629|Compound:3448-6466|FD-Score:2.97|P-value:5.33E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:3.56|P-value:4.65E-5 ID:SGTC_869|Compound:0564-0080|FD-Score:-3.12|P-value:4.11E-4 ID:SGTC_881|Compound:0833-0569|FD-Score:2.97|P-value:5.42E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:-3.28|P-value:2.16E-4 ID:SGTC_1012|Compound:4112-3522|FD-Score:2.94|P-value:6.01E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:-2.98|P-value:7.00E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:-4.01|P-value:8.04E-6 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:-3.43|P-value:1.15E-4 ID:SGTC_1097|Compound:itavastatin|FD-Score:-3.98|P-value:9.23E-6 ID:SGTC_1102|Compound:cgs 12066b|FD-Score:4.38|P-value:7.84E-7 ID:SGTC_1104|Compound:tacrolimus|FD-Score:-3.02|P-value:6.08E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.02|P-value:6.02E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:-4.43|P-value:9.40E-7 ID:SGTC_1129|Compound:3453-1384|FD-Score:4.02|P-value:5.06E-6 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:4.74|P-value:1.04E-7 ID:SGTC_1216|Compound:1165-0504|FD-Score:-2.92|P-value:8.64E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-3.48|P-value:9.22E-5 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.35|P-value:1.16E-4 ID:SGTC_1297|Compound:1068-0111|FD-Score:-3.25|P-value:2.41E-4 ID:SGTC_1672|Compound:st016598|FD-Score:3.92|P-value:8.38E-6 ID:SGTC_1722|Compound:st034304|FD-Score:-3.20|P-value:2.94E-4 ID:SGTC_1893|Compound:st059353|FD-Score:3.52|P-value:5.48E-5 ID:SGTC_1898|Compound:st060199|FD-Score:-3.12|P-value:4.08E-4 ID:SGTC_2035|Compound:5154463|FD-Score:2.91|P-value:6.69E-4 ID:SGTC_2052|Compound:5265182|FD-Score:-2.94|P-value:8.14E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:3.92|P-value:8.45E-6 ID:SGTC_2327|Compound:6189656|FD-Score:3.30|P-value:1.39E-4 ID:SGTC_2441|Compound:5730946|FD-Score:-3.01|P-value:6.30E-4 ID:SGTC_2444|Compound:5485484|FD-Score:4.26|P-value:1.52E-6 ID:SGTC_2620|Compound:irigenin|FD-Score:3.95|P-value:7.10E-6 ID:SGTC_2674|Compound:ligustilide|FD-Score:2.84|P-value:8.68E-4 ID:SGTC_2702|Compound:st077768|FD-Score:-3.08|P-value:4.83E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-4.25|P-value:2.34E-6 ID:SGTC_2978|Compound:9011784|FD-Score:-5.13|P-value:1.65E-8 ID:SGTC_3008|Compound:9078912|FD-Score:3.74|P-value:1.99E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-7.13|P-value:6.36E-15 ID:SGTC_3141|Compound:9094609|FD-Score:4.42|P-value:6.18E-7 ID:SGTC_3143|Compound:9086658|FD-Score:4.45|P-value:5.26E-7 ID:SGTC_3272|Compound:9137413|FD-Score:2.93|P-value:6.23E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:3.88|P-value:9.94E-6 ID:SGTC_234|Compound:nsc-17383|FD-Score:2.84|P-value:8.80E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.09|P-value:3.30E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:2.84|P-value:8.81E-4 ID:SGTC_437|Compound:s-farnesyl-l-cysteine methyl ester|FD-Score:-2.94|P-value:8.21E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:3.39|P-value:9.81E-5 ID:SGTC_592|Compound:k241-0073|FD-Score:3.13|P-value:2.79E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:3.74|P-value:2.01E-5 ID:SGTC_629|Compound:3448-6466|FD-Score:2.97|P-value:5.33E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:3.56|P-value:4.65E-5 ID:SGTC_869|Compound:0564-0080|FD-Score:-3.12|P-value:4.11E-4 ID:SGTC_881|Compound:0833-0569|FD-Score:2.97|P-value:5.42E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:-3.28|P-value:2.16E-4 ID:SGTC_1012|Compound:4112-3522|FD-Score:2.94|P-value:6.01E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:-2.98|P-value:7.00E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:-4.01|P-value:8.04E-6 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:-3.43|P-value:1.15E-4 ID:SGTC_1097|Compound:itavastatin|FD-Score:-3.98|P-value:9.23E-6 ID:SGTC_1102|Compound:cgs 12066b|FD-Score:4.38|P-value:7.84E-7 ID:SGTC_1104|Compound:tacrolimus|FD-Score:-3.02|P-value:6.08E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.02|P-value:6.02E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:-4.43|P-value:9.40E-7 ID:SGTC_1129|Compound:3453-1384|FD-Score:4.02|P-value:5.06E-6 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:4.74|P-value:1.04E-7 ID:SGTC_1216|Compound:1165-0504|FD-Score:-2.92|P-value:8.64E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-3.48|P-value:9.22E-5 ID:SGTC_1243|Compound:0416-0003|FD-Score:3.35|P-value:1.16E-4 ID:SGTC_1297|Compound:1068-0111|FD-Score:-3.25|P-value:2.41E-4 ID:SGTC_1672|Compound:st016598|FD-Score:3.92|P-value:8.38E-6 ID:SGTC_1722|Compound:st034304|FD-Score:-3.20|P-value:2.94E-4 ID:SGTC_1893|Compound:st059353|FD-Score:3.52|P-value:5.48E-5 ID:SGTC_1898|Compound:st060199|FD-Score:-3.12|P-value:4.08E-4 ID:SGTC_2035|Compound:5154463|FD-Score:2.91|P-value:6.69E-4 ID:SGTC_2052|Compound:5265182|FD-Score:-2.94|P-value:8.14E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:3.92|P-value:8.45E-6 ID:SGTC_2327|Compound:6189656|FD-Score:3.30|P-value:1.39E-4 ID:SGTC_2441|Compound:5730946|FD-Score:-3.01|P-value:6.30E-4 ID:SGTC_2444|Compound:5485484|FD-Score:4.26|P-value:1.52E-6 ID:SGTC_2620|Compound:irigenin|FD-Score:3.95|P-value:7.10E-6 ID:SGTC_2674|Compound:ligustilide|FD-Score:2.84|P-value:8.68E-4 ID:SGTC_2702|Compound:st077768|FD-Score:-3.08|P-value:4.83E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-4.25|P-value:2.34E-6 ID:SGTC_2978|Compound:9011784|FD-Score:-5.13|P-value:1.65E-8 ID:SGTC_3008|Compound:9078912|FD-Score:3.74|P-value:1.99E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-7.13|P-value:6.36E-15 ID:SGTC_3141|Compound:9094609|FD-Score:4.42|P-value:6.18E-7 ID:SGTC_3143|Compound:9086658|FD-Score:4.45|P-value:5.26E-7 ID:SGTC_3272|Compound:9137413|FD-Score:2.93|P-value:6.23E-4

Top fitness defect scores for YMR288W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1135 dihydrocapsaicin 4.74 536.7 nM 1.04E-7 4 1.09E-6 0.49
2 SGTC_3143 9086658 4.45 49.5 µM 5.26E-7 7 4.27E-6 0.04
3 SGTC_3141 9094609 4.42 49.5 µM 6.18E-7 iron homeostasis 10 4.89E-6 0.15
4 SGTC_1102 cgs 12066b 4.38 26.1 µM 7.84E-7 endomembrane recycling 2 5.99E-6 0.28
5 SGTC_2444 5485484 4.26 36.4 µM 1.52E-6 7 1.04E-5 0.03
6 SGTC_1129 3453-1384 4.02 223.0 µM 5.06E-6 16 2.89E-5 0.01
7 SGTC_2620 irigenin 3.95 100.0 µM 7.10E-6 15 3.85E-5 0.11
8 SGTC_1672 st016598 3.92 6.5 µM 8.38E-6 endomembrane recycling 19 4.43E-5 0.03
9 SGTC_2247 cerulenin 3.92 830.0 nM 8.45E-6 5 4.47E-5 0.12
10 SGTC_41 0478-0632 3.88 82.6 µM 9.94E-6 13 5.12E-5 0.05
11 SGTC_3008 9078912 3.74 71.4 µM 1.99E-5 4 9.21E-5 0.34
12 SGTC_618 k072-0232 3.74 33.2 µM 2.01E-5 60S ribosome export 8 9.28E-5 0.10
13 SGTC_845 0330-0138 3.56 4.5 µM 4.65E-5 8 1.89E-4 0.08
14 SGTC_1893 st059353 3.52 36.1 µM 5.48E-5 9 2.17E-4 0.10
15 SGTC_551 k048-0028 3.39 34.9 µM 9.81E-5 15 3.55E-4 0.04
16 SGTC_1243 0416-0003 3.35 5.7 µM 1.16E-4 PDR1 11 4.09E-4 0.04
17 SGTC_2327 6189656 3.30 200.0 µM 1.39E-4 14 4.77E-4 0.01
18 SGTC_592 k241-0073 3.13 46.5 µM 2.79E-4 9 8.59E-4 0.01
19 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 3.09 4.6 µM 3.30E-4 heat shock/prefoldin 36 9.91E-4 0.07
20 SGTC_629 3448-6466 2.97 76.7 µM 5.33E-4 15 0.00149 0.13

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.148 8.78E-18 YNL161W CBK1 het Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
0.145 3.51E-17 YKL021C MAK11 het Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats
0.140 4.94E-16 YKL083W_d YKL083W_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
0.135 4.78E-15 YKL042W SPC42 het Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
0.126 2.51E-13 YBR193C MED8 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.119 4.51E-12 YER018C SPC25 het Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
0.119 4.83E-12 YMR270C RRN9 het Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I
0.118 8.39E-12 YCR013C_d YCR013C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant
0.117 9.47E-12 YDL007W RPT2 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
0.106 8.46E-10 YHR118C ORC6 het Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p
0.105 1.03E-9 YPL151C PRP46 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
0.104 1.35E-9 YER126C NSA2 het Protein constituent of 66S pre-ribosomal particles, contributes to processing of the 27S pre-rRNA
0.104 1.41E-9 YER172C BRR2 het RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
0.103 2.27E-9 YKL125W RRN3 het Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
0.102 3.67E-9 YGL142C GPI10 het Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog