YMR296C / LCB1

Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine

Zygosity: Heterozygous strain
fixedexpanded
Profile for YMR296C / LCB1

Click on Significant Values for Screen Details ID:SGTC_287|Compound:k060-0039|FD-Score:-3.19|P-value:2.86E-4 ID:SGTC_306|Compound:1672-3436|FD-Score:3.67|P-value:3.24E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:-2.93|P-value:7.67E-4 ID:SGTC_406|Compound:estrone|FD-Score:3.48|P-value:7.71E-5 ID:SGTC_409|Compound:arachidonic acid|FD-Score:3.60|P-value:4.49E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:4.64|P-value:2.33E-7 ID:SGTC_525|Compound:k781-2675|FD-Score:3.00|P-value:5.43E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:-2.91|P-value:8.19E-4 ID:SGTC_583|Compound:1488-1091|FD-Score:-2.99|P-value:6.08E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:3.08|P-value:3.95E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:3.16|P-value:2.92E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:3.68|P-value:3.15E-5 ID:SGTC_671|Compound:0560-0003|FD-Score:10.80|P-value:6.44E-32 ID:SGTC_699|Compound:0375-0592|FD-Score:3.34|P-value:1.37E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:3.69|P-value:3.04E-5 ID:SGTC_868|Compound:0527-0199|FD-Score:-2.92|P-value:7.91E-4 ID:SGTC_870|Compound:0573-2213|FD-Score:-3.05|P-value:4.78E-4 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:-3.43|P-value:1.03E-4 ID:SGTC_1080|Compound:nisoldipine|FD-Score:5.36|P-value:2.83E-9 ID:SGTC_1147|Compound:3809-1045|FD-Score:-3.10|P-value:4.08E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:3.13|P-value:3.24E-4 ID:SGTC_1253|Compound:0550-0034|FD-Score:-3.50|P-value:7.52E-5 ID:SGTC_1254|Compound:0550-0035|FD-Score:4.74|P-value:1.29E-7 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:-2.95|P-value:7.20E-4 ID:SGTC_1297|Compound:1068-0111|FD-Score:-4.10|P-value:4.62E-6 ID:SGTC_1304|Compound:1165-0502|FD-Score:3.45|P-value:8.76E-5 ID:SGTC_1374|Compound:2889-5571|FD-Score:-3.00|P-value:5.96E-4 ID:SGTC_1724|Compound:st037281|FD-Score:-3.06|P-value:4.69E-4 ID:SGTC_1782|Compound:st039423|FD-Score:-3.31|P-value:1.72E-4 ID:SGTC_1864|Compound:st057645|FD-Score:-3.79|P-value:2.06E-5 ID:SGTC_1876|Compound:myriocin|FD-Score:18.60|P-value:5.90E-91 ID:SGTC_2018|Compound:5133068|FD-Score:-3.04|P-value:4.98E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-3.17|P-value:3.09E-4 ID:SGTC_2196|Compound:6573537|FD-Score:3.11|P-value:3.60E-4 ID:SGTC_2200|Compound:6600604|FD-Score:4.12|P-value:3.77E-6 ID:SGTC_2246|Compound:7293507|FD-Score:3.12|P-value:3.41E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:-4.05|P-value:6.00E-6 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-3.70|P-value:3.18E-5 ID:SGTC_2398|Compound:5228213|FD-Score:3.07|P-value:4.12E-4 ID:SGTC_2426|Compound:5582046|FD-Score:4.24|P-value:2.02E-6 ID:SGTC_2447|Compound:5741742|FD-Score:3.25|P-value:2.02E-4 ID:SGTC_2451|Compound:5856223|FD-Score:2.92|P-value:7.31E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.22|P-value:2.23E-6 ID:SGTC_2584|Compound:androsterone|FD-Score:2.90|P-value:7.98E-4 ID:SGTC_2850|Compound:9015429|FD-Score:-3.03|P-value:5.30E-4 ID:SGTC_2956|Compound:9076196|FD-Score:-3.01|P-value:5.58E-4 ID:SGTC_2959|Compound:9079155|FD-Score:3.84|P-value:1.48E-5 ID:SGTC_2991|Compound:9074669|FD-Score:3.16|P-value:2.96E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-5.47|P-value:1.58E-9 ID:SGTC_3138|Compound:9097216|FD-Score:-4.15|P-value:3.59E-6 ID:SGTC_3340|Compound:9146634|FD-Score:2.90|P-value:7.84E-4 ID:SGTC_287|Compound:k060-0039|FD-Score:-3.19|P-value:2.86E-4 ID:SGTC_306|Compound:1672-3436|FD-Score:3.67|P-value:3.24E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:-2.93|P-value:7.67E-4 ID:SGTC_406|Compound:estrone|FD-Score:3.48|P-value:7.71E-5 ID:SGTC_409|Compound:arachidonic acid|FD-Score:3.60|P-value:4.49E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:4.64|P-value:2.33E-7 ID:SGTC_525|Compound:k781-2675|FD-Score:3.00|P-value:5.43E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:-2.91|P-value:8.19E-4 ID:SGTC_583|Compound:1488-1091|FD-Score:-2.99|P-value:6.08E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:3.08|P-value:3.95E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:3.16|P-value:2.92E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:3.68|P-value:3.15E-5 ID:SGTC_671|Compound:0560-0003|FD-Score:10.80|P-value:6.44E-32 ID:SGTC_699|Compound:0375-0592|FD-Score:3.34|P-value:1.37E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:3.69|P-value:3.04E-5 ID:SGTC_868|Compound:0527-0199|FD-Score:-2.92|P-value:7.91E-4 ID:SGTC_870|Compound:0573-2213|FD-Score:-3.05|P-value:4.78E-4 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:-3.43|P-value:1.03E-4 ID:SGTC_1080|Compound:nisoldipine|FD-Score:5.36|P-value:2.83E-9 ID:SGTC_1147|Compound:3809-1045|FD-Score:-3.10|P-value:4.08E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:3.13|P-value:3.24E-4 ID:SGTC_1253|Compound:0550-0034|FD-Score:-3.50|P-value:7.52E-5 ID:SGTC_1254|Compound:0550-0035|FD-Score:4.74|P-value:1.29E-7 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:-2.95|P-value:7.20E-4 ID:SGTC_1297|Compound:1068-0111|FD-Score:-4.10|P-value:4.62E-6 ID:SGTC_1304|Compound:1165-0502|FD-Score:3.45|P-value:8.76E-5 ID:SGTC_1374|Compound:2889-5571|FD-Score:-3.00|P-value:5.96E-4 ID:SGTC_1724|Compound:st037281|FD-Score:-3.06|P-value:4.69E-4 ID:SGTC_1782|Compound:st039423|FD-Score:-3.31|P-value:1.72E-4 ID:SGTC_1864|Compound:st057645|FD-Score:-3.79|P-value:2.06E-5 ID:SGTC_1876|Compound:myriocin|FD-Score:18.60|P-value:5.90E-91 ID:SGTC_2018|Compound:5133068|FD-Score:-3.04|P-value:4.98E-4 ID:SGTC_2023|Compound:5144124|FD-Score:-3.17|P-value:3.09E-4 ID:SGTC_2196|Compound:6573537|FD-Score:3.11|P-value:3.60E-4 ID:SGTC_2200|Compound:6600604|FD-Score:4.12|P-value:3.77E-6 ID:SGTC_2246|Compound:7293507|FD-Score:3.12|P-value:3.41E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:-4.05|P-value:6.00E-6 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-3.70|P-value:3.18E-5 ID:SGTC_2398|Compound:5228213|FD-Score:3.07|P-value:4.12E-4 ID:SGTC_2426|Compound:5582046|FD-Score:4.24|P-value:2.02E-6 ID:SGTC_2447|Compound:5741742|FD-Score:3.25|P-value:2.02E-4 ID:SGTC_2451|Compound:5856223|FD-Score:2.92|P-value:7.31E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.22|P-value:2.23E-6 ID:SGTC_2584|Compound:androsterone|FD-Score:2.90|P-value:7.98E-4 ID:SGTC_2850|Compound:9015429|FD-Score:-3.03|P-value:5.30E-4 ID:SGTC_2956|Compound:9076196|FD-Score:-3.01|P-value:5.58E-4 ID:SGTC_2959|Compound:9079155|FD-Score:3.84|P-value:1.48E-5 ID:SGTC_2991|Compound:9074669|FD-Score:3.16|P-value:2.96E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-5.47|P-value:1.58E-9 ID:SGTC_3138|Compound:9097216|FD-Score:-4.15|P-value:3.59E-6 ID:SGTC_3340|Compound:9146634|FD-Score:2.90|P-value:7.84E-4

Top fitness defect scores for YMR296C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1876 myriocin 18.60 605.0 nM 5.90E-91 1 1.54E-77 10.70
2 SGTC_671 0560-0003 10.80 407.0 µM 6.44E-32 1 2.27E-27 4.99
3 SGTC_1080 nisoldipine 5.36 24.4 µM 2.83E-9 TSC3-RPN4 1 4.13E-8 0.69
4 SGTC_1254 0550-0035 4.74 264.0 µM 1.29E-7 5 1.07E-6 0.37
5 SGTC_457 grayanotoxin iii 4.64 135.0 µM 2.33E-7 17 1.78E-6 0.59
6 SGTC_2426 5582046 4.24 32.2 µM 2.02E-6 6 1.12E-5 0.16
7 SGTC_2567 aureobasidin a 4.22 60.0 nM 2.23E-6 39 1.22E-5 0.02
8 SGTC_2200 6600604 4.12 21.9 µM 3.77E-6 tubulin folding & SWR complex 5 1.91E-5 0.08
9 SGTC_2959 9079155 3.84 11.0 µM 1.48E-5 10 6.16E-5 0.14
10 SGTC_833 3852-0053 3.69 558.0 µM 3.04E-5 6 1.14E-4 0.05
11 SGTC_621 3584-0052 3.68 32.1 µM 3.15E-5 5 1.17E-4 0.16
12 SGTC_306 1672-3436 3.67 11.6 µM 3.24E-5 Golgi 4 1.20E-4 0.13
13 SGTC_409 arachidonic acid 3.60 100.0 µM 4.49E-5 4 1.59E-4 0.20
14 SGTC_406 estrone 3.48 100.0 µM 7.71E-5 2 2.52E-4 0.05
15 SGTC_1304 1165-0502 3.45 251.0 µM 8.76E-5 10 2.81E-4 0.13
16 SGTC_699 0375-0592 3.34 111.0 µM 1.37E-4 iron homeostasis 6 4.11E-4 0.16
17 SGTC_2447 5741742 3.25 33.8 µM 2.02E-4 14 5.74E-4 0.00
18 SGTC_618 k072-0232 3.16 33.2 µM 2.92E-4 60S ribosome export 13 7.89E-4 0.00
19 SGTC_2991 9074669 3.16 71.4 µM 2.96E-4 azole & statin 40 7.97E-4 0.03
20 SGTC_1218 1348-1416 3.13 30.7 µM 3.24E-4 SWF1 & branched chain AA biosynthesis 11 8.62E-4 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.554 2.13E-269 YGR212W SLI1 hom N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin
0.340 1.80E-91 YBR125C PTC4 hom Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity
0.293 1.56E-67 YDR071C PAA1 hom Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication
0.251 2.13E-49 YDR062W LCB2 het Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
0.234 4.31E-43 YGR213C RTA1 hom Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein
0.178 2.39E-25 YER130C_p YER130C_p hom Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress
0.175 1.82E-24 YJL213W YJL213W hom Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p
0.155 1.43E-19 YHR041C SRB2 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance
0.155 1.56E-19 YHR059W FYV4 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.144 4.48E-17 YDR094W_d YDR094W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2
0.139 5.64E-16 YJL134W LCB3 hom Long-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication
0.130 4.05E-14 YGL018C JAC1 het Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix
0.125 3.29E-13 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.122 1.51E-12 YGR104C SRB5 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance
0.121 1.88E-12 YGL213C SKI8 hom Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype