YMR298W / LIP1

Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis

Zygosity: Heterozygous strain
fixedexpanded
Profile for YMR298W / LIP1

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:-4.15|P-value:3.51E-6 ID:SGTC_62|Compound:0180-0423|FD-Score:3.91|P-value:1.25E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:3.42|P-value:1.16E-4 ID:SGTC_128|Compound:0906-2981|FD-Score:3.42|P-value:1.13E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:4.89|P-value:6.69E-8 ID:SGTC_139|Compound:0099-0334|FD-Score:4.91|P-value:5.74E-8 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.04|P-value:4.98E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:2.99|P-value:6.30E-4 ID:SGTC_230|Compound:MPP+|FD-Score:-3.27|P-value:1.96E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:-2.92|P-value:7.95E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:3.87|P-value:1.53E-5 ID:SGTC_361|Compound:000s-0510|FD-Score:4.97|P-value:4.13E-8 ID:SGTC_366|Compound:0181-0206|FD-Score:2.93|P-value:7.99E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-8.98|P-value:1.36E-22 ID:SGTC_550|Compound:4123-0385|FD-Score:3.03|P-value:5.38E-4 ID:SGTC_596|Compound:1155-0152|FD-Score:3.26|P-value:2.23E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:-4.08|P-value:5.12E-6 ID:SGTC_720|Compound:r052-1644|FD-Score:3.15|P-value:3.40E-4 ID:SGTC_739|Compound:1486-1183|FD-Score:3.17|P-value:3.13E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:2.96|P-value:7.18E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:-3.26|P-value:2.04E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:2.91|P-value:8.47E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:-2.93|P-value:7.63E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.65|P-value:4.22E-5 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.34|P-value:1.58E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:3.02|P-value:5.56E-4 ID:SGTC_1554|Compound:st069299|FD-Score:3.66|P-value:3.98E-5 ID:SGTC_1824|Compound:st053723|FD-Score:3.19|P-value:2.87E-4 ID:SGTC_1851|Compound:st056236|FD-Score:3.49|P-value:8.41E-5 ID:SGTC_1865|Compound:st057639|FD-Score:-5.05|P-value:2.38E-8 ID:SGTC_1868|Compound:st057644|FD-Score:3.01|P-value:5.91E-4 ID:SGTC_1875|Compound:nocodazole|FD-Score:3.28|P-value:2.05E-4 ID:SGTC_1882|Compound:5615643|FD-Score:6.39|P-value:2.77E-12 ID:SGTC_1974|Compound:st069448|FD-Score:2.93|P-value:7.91E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-4.61|P-value:3.14E-7 ID:SGTC_2237|Compound:6667747|FD-Score:3.26|P-value:2.16E-4 ID:SGTC_2286|Compound:7945697|FD-Score:5.20|P-value:9.88E-9 ID:SGTC_2319|Compound:6033012|FD-Score:-2.98|P-value:6.35E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.64|P-value:4.31E-5 ID:SGTC_2461|Compound:5755302|FD-Score:3.20|P-value:2.84E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.07|P-value:4.67E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:2.88|P-value:9.53E-4 ID:SGTC_2618|Compound:avocadyne|FD-Score:3.34|P-value:1.58E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:4.16|P-value:3.70E-6 ID:SGTC_2796|Compound:7949634|FD-Score:-3.31|P-value:1.73E-4 ID:SGTC_2800|Compound:7887935|FD-Score:3.36|P-value:1.44E-4 ID:SGTC_2936|Compound:9038235|FD-Score:-3.06|P-value:4.56E-4 ID:SGTC_3129|Compound:9127257|FD-Score:3.30|P-value:1.88E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:-4.15|P-value:3.51E-6 ID:SGTC_62|Compound:0180-0423|FD-Score:3.91|P-value:1.25E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:3.42|P-value:1.16E-4 ID:SGTC_128|Compound:0906-2981|FD-Score:3.42|P-value:1.13E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:4.89|P-value:6.69E-8 ID:SGTC_139|Compound:0099-0334|FD-Score:4.91|P-value:5.74E-8 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.04|P-value:4.98E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:2.99|P-value:6.30E-4 ID:SGTC_230|Compound:MPP+|FD-Score:-3.27|P-value:1.96E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:-2.92|P-value:7.95E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:3.87|P-value:1.53E-5 ID:SGTC_361|Compound:000s-0510|FD-Score:4.97|P-value:4.13E-8 ID:SGTC_366|Compound:0181-0206|FD-Score:2.93|P-value:7.99E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-8.98|P-value:1.36E-22 ID:SGTC_550|Compound:4123-0385|FD-Score:3.03|P-value:5.38E-4 ID:SGTC_596|Compound:1155-0152|FD-Score:3.26|P-value:2.23E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:-4.08|P-value:5.12E-6 ID:SGTC_720|Compound:r052-1644|FD-Score:3.15|P-value:3.40E-4 ID:SGTC_739|Compound:1486-1183|FD-Score:3.17|P-value:3.13E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:2.96|P-value:7.18E-4 ID:SGTC_1016|Compound:4226-1401|FD-Score:-3.26|P-value:2.04E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:2.91|P-value:8.47E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:-2.93|P-value:7.63E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.65|P-value:4.22E-5 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.34|P-value:1.58E-4 ID:SGTC_1507|Compound:4075-3230|FD-Score:3.02|P-value:5.56E-4 ID:SGTC_1554|Compound:st069299|FD-Score:3.66|P-value:3.98E-5 ID:SGTC_1824|Compound:st053723|FD-Score:3.19|P-value:2.87E-4 ID:SGTC_1851|Compound:st056236|FD-Score:3.49|P-value:8.41E-5 ID:SGTC_1865|Compound:st057639|FD-Score:-5.05|P-value:2.38E-8 ID:SGTC_1868|Compound:st057644|FD-Score:3.01|P-value:5.91E-4 ID:SGTC_1875|Compound:nocodazole|FD-Score:3.28|P-value:2.05E-4 ID:SGTC_1882|Compound:5615643|FD-Score:6.39|P-value:2.77E-12 ID:SGTC_1974|Compound:st069448|FD-Score:2.93|P-value:7.91E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-4.61|P-value:3.14E-7 ID:SGTC_2237|Compound:6667747|FD-Score:3.26|P-value:2.16E-4 ID:SGTC_2286|Compound:7945697|FD-Score:5.20|P-value:9.88E-9 ID:SGTC_2319|Compound:6033012|FD-Score:-2.98|P-value:6.35E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.64|P-value:4.31E-5 ID:SGTC_2461|Compound:5755302|FD-Score:3.20|P-value:2.84E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.07|P-value:4.67E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:2.88|P-value:9.53E-4 ID:SGTC_2618|Compound:avocadyne|FD-Score:3.34|P-value:1.58E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:4.16|P-value:3.70E-6 ID:SGTC_2796|Compound:7949634|FD-Score:-3.31|P-value:1.73E-4 ID:SGTC_2800|Compound:7887935|FD-Score:3.36|P-value:1.44E-4 ID:SGTC_2936|Compound:9038235|FD-Score:-3.06|P-value:4.56E-4 ID:SGTC_3129|Compound:9127257|FD-Score:3.30|P-value:1.88E-4

Top fitness defect scores for YMR298W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1882 5615643 6.39 20.0 µM 2.77E-12 TRP & mitochondrial translation 2 8.52E-11 1.63
2 SGTC_2286 7945697 5.20 125.0 µM 9.88E-9 4 9.78E-8 0.40
3 SGTC_361 000s-0510 4.97 1.4 µM 4.13E-8 4 3.36E-7 0.64
4 SGTC_139 0099-0334 4.91 718.6 µM 5.74E-8 2 4.45E-7 0.54
5 SGTC_130 0136-0219 4.89 337.7 µM 6.69E-8 3 5.09E-7 0.04
6 SGTC_2774 tamoxifen 4.16 10.9 µM 3.70E-6 6 1.62E-5 0.23
7 SGTC_62 0180-0423 3.91 3.2 µM 1.25E-5 60S ribosome export 11 4.63E-5 0.08
8 SGTC_352 k048-0007 3.87 5.1 µM 1.53E-5 ERAD & cell cycle 12 5.52E-5 0.12
9 SGTC_1554 st069299 3.66 38.7 µM 3.98E-5 6 1.27E-4 0.38
10 SGTC_1298 1079-1278 3.65 263.0 µM 4.22E-5 DNA damage response 7 1.33E-4 0.19
11 SGTC_2415 st077333 3.64 57.7 µM 4.31E-5 19 1.36E-4 0.17
12 SGTC_1851 st056236 3.49 18.9 µM 8.41E-5 7 2.42E-4 0.03
13 SGTC_128 0906-2981 3.42 13.4 µM 1.13E-4 15 3.11E-4 0.03
14 SGTC_103 0844-0104 3.42 51.8 µM 1.16E-4 ERAD & cell cycle 63 3.19E-4 0.03
15 SGTC_2800 7887935 3.36 81.8 µM 1.44E-4 RPP1 & pyrimidine depletion 14 3.86E-4 0.01
16 SGTC_2618 avocadyne 3.34 43.4 µM 1.58E-4 6 4.17E-4 0.16
17 SGTC_1333 1482-0047 3.34 286.0 µM 1.58E-4 10 4.18E-4 0.01
18 SGTC_3129 9127257 3.30 49.5 µM 1.88E-4 13 4.86E-4 0.16
19 SGTC_1875 nocodazole 3.28 6.0 µM 2.05E-4 tubulin folding & SWR complex 24 5.23E-4 0.21
20 SGTC_2237 6667747 3.26 47.2 µM 2.16E-4 5 5.48E-4 0.15

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.205 4.24E-33 YOR304W ISW2 hom ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth
0.145 2.96E-17 YML006C GIS4 hom CAAX box containing protein of unknown function, proposed to be involved in the RAS/cAMP signaling pathway
0.122 1.25E-12 YJR116W_p TDA4_p hom Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele
0.111 1.14E-10 YPR179C HDA3 hom Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p
0.108 3.19E-10 YGR047C TFC4 het One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102
0.108 3.56E-10 YGL094C PAN2 hom Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
0.107 4.97E-10 YDR295C HDA2 hom Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance
0.105 1.15E-9 YKL008C LAC1 hom Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p
0.101 4.52E-9 YOL065C INP54 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
0.101 4.81E-9 YER085C_p YER085C_p hom Putative protein of unknown function
0.096 2.72E-8 YHR065C RRP3 het Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity
0.088 2.98E-7 YMR135W-A_d YMR135W-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.087 4.69E-7 YGR116W SPT6 het Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
0.086 5.72E-7 YNL140C_p YNL140C_p hom Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C
0.084 9.91E-7 YOR363C PIP2 hom Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication