YMR300C / ADE4

Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR300C / ADE4

Click on Significant Values for Screen Details ID:SGTC_59|Compound:0330-0160|FD-Score:3.47|P-value:3.94E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:3.61|P-value:2.41E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:17.00|P-value:1.20E-61 ID:SGTC_140|Compound:0062-0056|FD-Score:3.46|P-value:4.11E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:-3.36|P-value:5.41E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:5.36|P-value:1.03E-7 ID:SGTC_355|Compound:1311-2252|FD-Score:-3.25|P-value:7.82E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.21|P-value:8.87E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:4.26|P-value:1.85E-5 ID:SGTC_686|Compound:1319-0111|FD-Score:3.81|P-value:1.14E-4 ID:SGTC_869|Compound:0564-0080|FD-Score:3.20|P-value:9.80E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:3.69|P-value:1.75E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:4.33|P-value:1.37E-5 ID:SGTC_1006|Compound:4099-6547|FD-Score:3.23|P-value:8.86E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:4.10|P-value:3.57E-5 ID:SGTC_1175|Compound:0741-0056|FD-Score:-3.83|P-value:9.48E-5 ID:SGTC_1176|Compound:0848-0131|FD-Score:4.91|P-value:9.83E-7 ID:SGTC_1190|Compound:1486-1332|FD-Score:4.66|P-value:3.19E-6 ID:SGTC_1275|Compound:0867-0008|FD-Score:5.77|P-value:1.16E-8 ID:SGTC_1363|Compound:1636-0063|FD-Score:4.55|P-value:5.20E-6 ID:SGTC_1392|Compound:3455-1037|FD-Score:4.64|P-value:3.44E-6 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.26|P-value:8.12E-4 ID:SGTC_1455|Compound:4262-0120|FD-Score:4.33|P-value:1.36E-5 ID:SGTC_1456|Compound:k015-0012|FD-Score:-3.49|P-value:3.35E-4 ID:SGTC_1516|Compound:st013856|FD-Score:-3.81|P-value:1.04E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:4.41|P-value:9.96E-6 ID:SGTC_1640|Compound:st009078|FD-Score:3.64|P-value:2.16E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:3.86|P-value:9.27E-5 ID:SGTC_1812|Compound:st052057|FD-Score:3.84|P-value:9.98E-5 ID:SGTC_2019|Compound:4016004|FD-Score:4.51|P-value:6.20E-6 ID:SGTC_2049|Compound:5262831|FD-Score:-3.27|P-value:7.27E-4 ID:SGTC_2053|Compound:5265679|FD-Score:3.21|P-value:9.51E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.37|P-value:5.48E-4 ID:SGTC_2230|Compound:bromochlorosalicylanilide|FD-Score:3.54|P-value:3.10E-4 ID:SGTC_2235|Compound:salicylic acid|FD-Score:5.43|P-value:7.18E-8 ID:SGTC_2245|Compound:6637752|FD-Score:3.28|P-value:7.59E-4 ID:SGTC_2379|Compound:9035213|FD-Score:3.44|P-value:4.37E-4 ID:SGTC_2385|Compound:9074692|FD-Score:4.08|P-value:3.87E-5 ID:SGTC_2400|Compound:5203494|FD-Score:3.32|P-value:6.61E-4 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:4.81|P-value:1.61E-6 ID:SGTC_2511|Compound:berberine|FD-Score:4.91|P-value:1.00E-6 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.26|P-value:8.14E-4 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:3.57|P-value:2.80E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:4.99|P-value:6.88E-7 ID:SGTC_2561|Compound:acetosyringone|FD-Score:5.71|P-value:1.61E-8 ID:SGTC_2576|Compound:guaiol|FD-Score:6.58|P-value:8.84E-11 ID:SGTC_2586|Compound:curcumin|FD-Score:3.62|P-value:2.31E-4 ID:SGTC_2673|Compound:kobusone|FD-Score:3.55|P-value:3.01E-4 ID:SGTC_2782|Compound:7736369|FD-Score:4.51|P-value:6.16E-6 ID:SGTC_2789|Compound:5281112|FD-Score:5.28|P-value:1.58E-7 ID:SGTC_2982|Compound:9019404|FD-Score:4.53|P-value:5.84E-6 ID:SGTC_3031|Compound:9088762|FD-Score:4.36|P-value:1.23E-5 ID:SGTC_3042|Compound:9091578|FD-Score:4.15|P-value:2.89E-5 ID:SGTC_3065|Compound:9113888|FD-Score:3.49|P-value:3.71E-4 ID:SGTC_3083|Compound:9117465|FD-Score:3.47|P-value:3.91E-4 ID:SGTC_3135|Compound:9127742|FD-Score:3.70|P-value:1.70E-4 ID:SGTC_3181|Compound:9108277|FD-Score:3.62|P-value:2.34E-4 ID:SGTC_3202|Compound:9113761|FD-Score:3.64|P-value:2.14E-4 ID:SGTC_3214|Compound:9129694|FD-Score:4.13|P-value:3.22E-5 ID:SGTC_3216|Compound:9129716|FD-Score:-4.00|P-value:4.92E-5 ID:SGTC_3242|Compound:9133940|FD-Score:4.04|P-value:4.63E-5 ID:SGTC_3244|Compound:9134489|FD-Score:8.17|P-value:1.16E-15 ID:SGTC_59|Compound:0330-0160|FD-Score:3.47|P-value:3.94E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:3.61|P-value:2.41E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:17.00|P-value:1.20E-61 ID:SGTC_140|Compound:0062-0056|FD-Score:3.46|P-value:4.11E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:-3.36|P-value:5.41E-4 ID:SGTC_341|Compound:1486-1430|FD-Score:5.36|P-value:1.03E-7 ID:SGTC_355|Compound:1311-2252|FD-Score:-3.25|P-value:7.82E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:-3.21|P-value:8.87E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:4.26|P-value:1.85E-5 ID:SGTC_686|Compound:1319-0111|FD-Score:3.81|P-value:1.14E-4 ID:SGTC_869|Compound:0564-0080|FD-Score:3.20|P-value:9.80E-4 ID:SGTC_941|Compound:3237-0776|FD-Score:3.69|P-value:1.75E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:4.33|P-value:1.37E-5 ID:SGTC_1006|Compound:4099-6547|FD-Score:3.23|P-value:8.86E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:4.10|P-value:3.57E-5 ID:SGTC_1175|Compound:0741-0056|FD-Score:-3.83|P-value:9.48E-5 ID:SGTC_1176|Compound:0848-0131|FD-Score:4.91|P-value:9.83E-7 ID:SGTC_1190|Compound:1486-1332|FD-Score:4.66|P-value:3.19E-6 ID:SGTC_1275|Compound:0867-0008|FD-Score:5.77|P-value:1.16E-8 ID:SGTC_1363|Compound:1636-0063|FD-Score:4.55|P-value:5.20E-6 ID:SGTC_1392|Compound:3455-1037|FD-Score:4.64|P-value:3.44E-6 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.26|P-value:8.12E-4 ID:SGTC_1455|Compound:4262-0120|FD-Score:4.33|P-value:1.36E-5 ID:SGTC_1456|Compound:k015-0012|FD-Score:-3.49|P-value:3.35E-4 ID:SGTC_1516|Compound:st013856|FD-Score:-3.81|P-value:1.04E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:4.41|P-value:9.96E-6 ID:SGTC_1640|Compound:st009078|FD-Score:3.64|P-value:2.16E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:3.86|P-value:9.27E-5 ID:SGTC_1812|Compound:st052057|FD-Score:3.84|P-value:9.98E-5 ID:SGTC_2019|Compound:4016004|FD-Score:4.51|P-value:6.20E-6 ID:SGTC_2049|Compound:5262831|FD-Score:-3.27|P-value:7.27E-4 ID:SGTC_2053|Compound:5265679|FD-Score:3.21|P-value:9.51E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.37|P-value:5.48E-4 ID:SGTC_2230|Compound:bromochlorosalicylanilide|FD-Score:3.54|P-value:3.10E-4 ID:SGTC_2235|Compound:salicylic acid|FD-Score:5.43|P-value:7.18E-8 ID:SGTC_2245|Compound:6637752|FD-Score:3.28|P-value:7.59E-4 ID:SGTC_2379|Compound:9035213|FD-Score:3.44|P-value:4.37E-4 ID:SGTC_2385|Compound:9074692|FD-Score:4.08|P-value:3.87E-5 ID:SGTC_2400|Compound:5203494|FD-Score:3.32|P-value:6.61E-4 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:4.81|P-value:1.61E-6 ID:SGTC_2511|Compound:berberine|FD-Score:4.91|P-value:1.00E-6 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.26|P-value:8.14E-4 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:3.57|P-value:2.80E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:4.99|P-value:6.88E-7 ID:SGTC_2561|Compound:acetosyringone|FD-Score:5.71|P-value:1.61E-8 ID:SGTC_2576|Compound:guaiol|FD-Score:6.58|P-value:8.84E-11 ID:SGTC_2586|Compound:curcumin|FD-Score:3.62|P-value:2.31E-4 ID:SGTC_2673|Compound:kobusone|FD-Score:3.55|P-value:3.01E-4 ID:SGTC_2782|Compound:7736369|FD-Score:4.51|P-value:6.16E-6 ID:SGTC_2789|Compound:5281112|FD-Score:5.28|P-value:1.58E-7 ID:SGTC_2982|Compound:9019404|FD-Score:4.53|P-value:5.84E-6 ID:SGTC_3031|Compound:9088762|FD-Score:4.36|P-value:1.23E-5 ID:SGTC_3042|Compound:9091578|FD-Score:4.15|P-value:2.89E-5 ID:SGTC_3065|Compound:9113888|FD-Score:3.49|P-value:3.71E-4 ID:SGTC_3083|Compound:9117465|FD-Score:3.47|P-value:3.91E-4 ID:SGTC_3135|Compound:9127742|FD-Score:3.70|P-value:1.70E-4 ID:SGTC_3181|Compound:9108277|FD-Score:3.62|P-value:2.34E-4 ID:SGTC_3202|Compound:9113761|FD-Score:3.64|P-value:2.14E-4 ID:SGTC_3214|Compound:9129694|FD-Score:4.13|P-value:3.22E-5 ID:SGTC_3216|Compound:9129716|FD-Score:-4.00|P-value:4.92E-5 ID:SGTC_3242|Compound:9133940|FD-Score:4.04|P-value:4.63E-5 ID:SGTC_3244|Compound:9134489|FD-Score:8.17|P-value:1.16E-15

Top fitness defect scores for YMR300C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_123 0083-0120 17.00 66.7 µM 1.20E-61 3 2.25E-65
2 SGTC_3244 9134489 8.17 49.5 µM 1.16E-15 6 1.50E-16
3 SGTC_2576 guaiol 6.58 23.1 µM 8.84E-11 5 2.29E-11
4 SGTC_1275 0867-0008 5.77 21.1 µM 1.16E-8 2 4.06E-9
5 SGTC_2561 acetosyringone 5.71 100.0 µM 1.61E-8 6 5.74E-9
6 SGTC_2235 salicylic acid 5.43 500.0 µM 7.18E-8 1 2.81E-8
7 SGTC_341 1486-1430 5.36 230.0 µM 1.03E-7 6 4.12E-8
8 SGTC_2789 5281112 5.28 71.4 µM 1.58E-7 11 6.50E-8
9 SGTC_2553 4-methylesculetin 4.99 85.1 µM 6.88E-7 6 3.09E-7
10 SGTC_1176 0848-0131 4.91 83.3 µM 9.83E-7 Golgi 10 4.52E-7
11 SGTC_2511 berberine 4.91 10.5 µM 1.00E-6 60S ribosome export 8 4.62E-7
12 SGTC_2497 7-hydroxyflavone 4.81 100.0 µM 1.61E-6 8 7.61E-7
13 SGTC_1190 1486-1332 4.66 13.0 µM 3.19E-6 4 1.57E-6
14 SGTC_1392 3455-1037 4.64 33.9 µM 3.44E-6 6 1.71E-6
15 SGTC_1363 1636-0063 4.55 10.4 µM 5.20E-6 Golgi 16 2.65E-6
16 SGTC_2982 9019404 4.53 26.0 µM 5.84E-6 14 2.99E-6
17 SGTC_2782 7736369 4.51 71.4 µM 6.16E-6 21 3.17E-6
18 SGTC_2019 4016004 4.51 108.0 µM 6.20E-6 12 3.19E-6
19 SGTC_1517 deoxycholic acid methyl ester 4.41 49.2 µM 9.96E-6 4 5.27E-6
20 SGTC_3031 9088762 4.36 49.5 µM 1.23E-5 10 6.57E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.416 1.37E-140 YGR204W ADE3 hom Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
0.300 1.19E-70 YGL234W ADE5,7 hom Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities
0.279 4.93E-61 YLR058C SHM2 hom Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis
0.274 6.93E-59 YGR061C ADE6 hom Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
0.229 6.67E-41 YDR408C ADE8 hom Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
0.138 1.14E-15 YKR080W MTD1 hom NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline
0.129 7.63E-14 YMR120C ADE17 hom Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine
0.115 2.83E-11 YJR046W TAH11 het DNA replication licensing factor, required for pre-replication complex assembly
0.105 9.28E-10 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.102 2.87E-9 YLR264W RPS28B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication
0.101 4.16E-9 YGR239C PEX21 hom Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress
0.100 7.33E-9 YGR273C_p YGR273C_p hom Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene
0.090 1.63E-7 YLR140W_d YLR140W_d het Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I
0.085 7.48E-7 YLL044W_d YLL044W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant
0.085 8.77E-7 YHR010W RPL27A hom Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication