HIPHOP chemogenomics database

YMR314W / PRE5

Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain

fixedexpanded
Profile for YMR314W / PRE5

Click on Significant Values for Screen Details

Top fitness defect scores for YMR314W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_64	000l-0018	21.80	272.1 µM	1.16E-97	ubiquinone biosynthesis & proteasome	2	1.38E-105	2.27
2	SGTC_1597	7-benzyloxygramine	14.10	47.5 µM	2.88E-42	calcium & mitochondrial duress	10	1.46E-45	0.55
3	SGTC_2250	alverine citrate	10.90	130.0 µM	6.02E-26		8	6.85E-28	1.29
4	SGTC_187	1488-2104	8.03	32.7 µM	5.30E-15	calcium & mitochondrial duress	2	4.74E-16	1.51
5	SGTC_1712	st032288	7.90	53.4 µM	1.40E-14	calcium & mitochondrial duress	8	1.35E-15	0.24
6	SGTC_1880	MG-132	6.78	525.0 µM	3.24E-11		5	5.85E-12	0.02
7	SGTC_137	0438-0332	6.71	85.4 µM	5.24E-11	ubiquinone biosynthesis & proteasome	18	9.83E-12	0.82
8	SGTC_2249	haloperidol	6.67	116.4 µM	6.63E-11	ergosterol depletion effects on membrane	11	1.27E-11	0.04
9	SGTC_165	k038-0023	6.40	64.4 µM	3.66E-10	ubiquinone biosynthesis & proteasome	7	8.02E-11	0.13
10	SGTC_161	4065-0411	6.10	162.2 µM	2.07E-9		6	5.21E-10	0.32
11	SGTC_1266	0828-0227	6.06	32.9 µM	2.59E-9		3	6.63E-10	0.88
12	SGTC_216	k038-0035	6.01	28.4 µM	3.54E-9	ubiquinone biosynthesis & proteasome	4	9.31E-10	0.22
13	SGTC_467	ro 31-8220	5.39	72.8 µM	1.01E-7	calcium & mitochondrial duress	9	3.47E-8	0.35
14	SGTC_3018	9082602	5.22	49.5 µM	2.45E-7		3	8.99E-8	0.68
15	SGTC_1454	4239-0054	5.21	42.6 µM	2.52E-7		2	9.28E-8	0.62
16	SGTC_539	4859-0462	5.20	2.5 µM	2.67E-7	RNA pol III & RNase P/MRP	11	9.86E-8	0.16
17	SGTC_2658	polymyxin b sulfate	5.18	77.0 µM	2.93E-7	NEO1	8	1.09E-7	0.48
18	SGTC_294	k038-0071	5.09	157.0 µM	4.68E-7	ubiquinone biosynthesis & proteasome	10	1.81E-7	0.94
19	SGTC_312	4092-0653	4.90	6.8 µM	1.15E-6	ergosterol depletion effects on membrane	21	4.75E-7	0.00
20	SGTC_268	camptothecin	4.88	39.6 µM	1.26E-6	DNA damage response	32	5.26E-7	0.19

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.581	4.21E-302	YER094C	PUP3	het	Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
0.527	1.06E-238	YOL038W	PRE6	het	Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
0.516	2.54E-227	YFR050C	PRE4	het	Beta 7 subunit of the 20S proteasome
0.493	8.04E-205	YML092C	PRE8	het	Alpha 2 subunit of the 20S proteasome
0.485	1.65E-197	YOR362C	PRE10	het	Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.482	4.99E-195	YGL011C	SCL1	het	Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
0.445	4.84E-163	YER012W	PRE1	het	Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
0.442	1.11E-159	YBL041W	PRE7	het	Beta 6 subunit of the 20S proteasome
0.434	7.54E-154	YGR253C	PUP2	het	Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.394	1.32E-124	YLR021W	IRC25	hom	Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.339	8.05E-91	YNL111C	CYB5	hom	Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
0.332	2.91E-87	YJL001W	PRE3	het	Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
0.295	2.52E-68	YPR103W	PRE2	het	Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
0.290	1.13E-65	YGL119W	COQ8	hom	Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.286	6.72E-64	YJL165C	HAL5	hom	Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication