HIPHOP chemogenomics database

YMR316W / DIA1

Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern

Zygosity: Homozygous strain

fixedexpanded
Profile for YMR316W / DIA1

Click on Significant Values for Screen Details

Top fitness defect scores for YMR316W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_58	0957-0393	12.50	9.0 µM	5.46E-33	cell wall	46	5.13E-36
2	SGTC_767	0929-0023	7.27	86.0 µM	2.16E-12	TRP & mitochondrial translation	15	1.82E-13
3	SGTC_138	0850-0259	6.86	40.9 µM	3.15E-11	cell wall	79	3.43E-12
4	SGTC_729	4092-0585	6.22	94.1 µM	1.57E-9		2	2.47E-10
5	SGTC_1680	diphenhydramine	5.98	100.0 µM	6.34E-9	NEO1	6	1.14E-9
6	SGTC_1942	st076330	5.97	65.2 µM	6.48E-9		3	1.17E-9
7	SGTC_2605	digitonin	5.96	300.0 nM	6.87E-9		3	1.25E-9
8	SGTC_1661	st013063	5.81	9.7 µM	1.62E-8		6	3.20E-9
9	SGTC_1683	st010134	5.19	710.0 nM	4.00E-7		9	1.07E-7
10	SGTC_2697	mebhydrolin	5.00	72.4 µM	9.70E-7	NEO1	45	2.82E-7
11	SGTC_190	3970-0790	4.99	3.7 µM	1.04E-6		1	3.03E-7
12	SGTC_763	3453-1109	4.96	128.0 µM	1.17E-6		6	3.45E-7
13	SGTC_513	4245-1575	4.69	41.7 µM	4.06E-6	unfolded protein response	85	1.35E-6
14	SGTC_3129	9127257	4.66	49.5 µM	4.61E-6		9	1.55E-6
15	SGTC_1105	epirubicin	4.37	18.1 µM	1.65E-5	anthracycline transcription coupled DNA repair	14	6.28E-6
16	SGTC_759	3486-0358	4.36	89.3 µM	1.67E-5		14	6.36E-6
17	SGTC_1715	st032160	4.36	10.2 µM	1.71E-5		35	6.54E-6
18	SGTC_1385	3091-4834	4.28	14.1 µM	2.39E-5	RPP1 & pyrimidine depletion	14	9.43E-6
19	SGTC_1543	azetidyl-2-carboxylic acid	4.24	198.0 µM	2.82E-5		7	1.13E-5
20	SGTC_3235	9132602	4.23	49.5 µM	2.87E-5		17	1.15E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.265	3.90E-55	YAL015C	NTG1	hom	DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.252	1.39E-49	YDR205W	MSC2	hom	Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.237	3.91E-44	YOR231W	MKK1	hom	MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.233	1.79E-42	YJR054W	KCH1	hom	Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.230	1.29E-41	YLR331C_d	JIP3_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.225	1.13E-39	YLR150W	STM1	hom	Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.214	4.71E-36	YGR058W	PEF1	hom	Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.210	6.58E-35	YKL076C_d	PSY1_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.205	2.84E-33	YNL314W	DAL82	hom	Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.202	3.38E-32	YML057C-A_d	YML057C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W
0.196	2.24E-30	YJL059W	YHC3	hom	Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis)
0.192	2.53E-29	YBR091C	TIM12	het	Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
0.191	5.64E-29	YKL033W-A_p	YKL033W-A_p	hom	Putative protein of unknown function; similar to uncharacterized proteins from other fungi
0.189	2.66E-28	YIL160C	POT1	hom	3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.187	8.89E-28	YIL148W	RPL40A	hom	Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress