YNL001W / DOM34

Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL001W / DOM34

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:4.51|P-value:4.56E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.68|P-value:1.18E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:3.54|P-value:2.53E-4 ID:SGTC_88|Compound:0180-0332|FD-Score:-3.26|P-value:5.59E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:6.80|P-value:9.78E-12 ID:SGTC_156|Compound:3474-0145|FD-Score:4.73|P-value:1.63E-6 ID:SGTC_280|Compound:3448-3607|FD-Score:3.67|P-value:1.56E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.48|P-value:2.49E-4 ID:SGTC_393|Compound:acivicin|FD-Score:3.23|P-value:7.52E-4 ID:SGTC_825|Compound:1988-2224|FD-Score:3.70|P-value:1.38E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:4.15|P-value:2.22E-5 ID:SGTC_884|Compound:0851-0618|FD-Score:-3.39|P-value:3.47E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:3.19|P-value:8.70E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:5.10|P-value:2.58E-7 ID:SGTC_1181|Compound:1222-0449|FD-Score:4.07|P-value:3.09E-5 ID:SGTC_1879|Compound:gemcitabine|FD-Score:3.38|P-value:4.54E-4 ID:SGTC_2296|Compound:7971656|FD-Score:-3.51|P-value:2.30E-4 ID:SGTC_2297|Compound:7971671|FD-Score:3.42|P-value:3.98E-4 ID:SGTC_2342|Compound:7990637|FD-Score:3.71|P-value:1.36E-4 ID:SGTC_2349|Compound:9023730|FD-Score:3.36|P-value:4.88E-4 ID:SGTC_2401|Compound:5128683|FD-Score:-4.11|P-value:2.02E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.49|P-value:2.47E-4 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:5.52|P-value:2.75E-8 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.42|P-value:6.95E-6 ID:SGTC_2701|Compound:st077767|FD-Score:-4.30|P-value:8.99E-6 ID:SGTC_2759|Compound:disulfiram|FD-Score:3.33|P-value:5.34E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:3.21|P-value:8.31E-4 ID:SGTC_2896|Compound:9052656|FD-Score:4.30|P-value:1.18E-5 ID:SGTC_3136|Compound:9095672|FD-Score:4.12|P-value:2.61E-5 ID:SGTC_3152|Compound:9099253|FD-Score:-3.58|P-value:1.72E-4 ID:SGTC_3194|Compound:9111903|FD-Score:4.23|P-value:1.56E-5 ID:SGTC_3207|Compound:9114163|FD-Score:3.48|P-value:3.11E-4 ID:SGTC_3211|Compound:9128781|FD-Score:5.49|P-value:3.15E-8 ID:SGTC_3229|Compound:9132204|FD-Score:3.54|P-value:2.51E-4 ID:SGTC_3234|Compound:9132280|FD-Score:3.92|P-value:5.79E-5 ID:SGTC_3251|Compound:9135047|FD-Score:-4.57|P-value:2.57E-6 ID:SGTC_43|Compound:0570-0105|FD-Score:4.51|P-value:4.56E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.68|P-value:1.18E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:3.54|P-value:2.53E-4 ID:SGTC_88|Compound:0180-0332|FD-Score:-3.26|P-value:5.59E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:6.80|P-value:9.78E-12 ID:SGTC_156|Compound:3474-0145|FD-Score:4.73|P-value:1.63E-6 ID:SGTC_280|Compound:3448-3607|FD-Score:3.67|P-value:1.56E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.48|P-value:2.49E-4 ID:SGTC_393|Compound:acivicin|FD-Score:3.23|P-value:7.52E-4 ID:SGTC_825|Compound:1988-2224|FD-Score:3.70|P-value:1.38E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:4.15|P-value:2.22E-5 ID:SGTC_884|Compound:0851-0618|FD-Score:-3.39|P-value:3.47E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:3.19|P-value:8.70E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:5.10|P-value:2.58E-7 ID:SGTC_1181|Compound:1222-0449|FD-Score:4.07|P-value:3.09E-5 ID:SGTC_1879|Compound:gemcitabine|FD-Score:3.38|P-value:4.54E-4 ID:SGTC_2296|Compound:7971656|FD-Score:-3.51|P-value:2.30E-4 ID:SGTC_2297|Compound:7971671|FD-Score:3.42|P-value:3.98E-4 ID:SGTC_2342|Compound:7990637|FD-Score:3.71|P-value:1.36E-4 ID:SGTC_2349|Compound:9023730|FD-Score:3.36|P-value:4.88E-4 ID:SGTC_2401|Compound:5128683|FD-Score:-4.11|P-value:2.02E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.49|P-value:2.47E-4 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:5.52|P-value:2.75E-8 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.42|P-value:6.95E-6 ID:SGTC_2701|Compound:st077767|FD-Score:-4.30|P-value:8.99E-6 ID:SGTC_2759|Compound:disulfiram|FD-Score:3.33|P-value:5.34E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:3.21|P-value:8.31E-4 ID:SGTC_2896|Compound:9052656|FD-Score:4.30|P-value:1.18E-5 ID:SGTC_3136|Compound:9095672|FD-Score:4.12|P-value:2.61E-5 ID:SGTC_3152|Compound:9099253|FD-Score:-3.58|P-value:1.72E-4 ID:SGTC_3194|Compound:9111903|FD-Score:4.23|P-value:1.56E-5 ID:SGTC_3207|Compound:9114163|FD-Score:3.48|P-value:3.11E-4 ID:SGTC_3211|Compound:9128781|FD-Score:5.49|P-value:3.15E-8 ID:SGTC_3229|Compound:9132204|FD-Score:3.54|P-value:2.51E-4 ID:SGTC_3234|Compound:9132280|FD-Score:3.92|P-value:5.79E-5 ID:SGTC_3251|Compound:9135047|FD-Score:-4.57|P-value:2.57E-6

Top fitness defect scores for YNL001W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_146 3448-8650 6.80 53.1 µM 9.78E-12 NEO1-PIK1 41 5.08E-12
2 SGTC_2633 5,7-dihydroxyisoflavone 5.52 100.0 µM 2.75E-8 8 1.72E-8
3 SGTC_3211 9128781 5.49 49.5 µM 3.15E-8 25 1.98E-8
4 SGTC_1129 3453-1384 5.10 223.0 µM 2.58E-7 18 1.71E-7
5 SGTC_156 3474-0145 4.73 5.6 µM 1.63E-6 endomembrane recycling 38 1.13E-6
6 SGTC_43 0570-0105 4.51 72.5 µM 4.56E-6 ERG2 71 3.24E-6
7 SGTC_2688 kanamycin b 4.42 96.7 µM 6.95E-6 calcium & mitochondrial duress 304 4.99E-6
8 SGTC_2896 9052656 4.30 71.4 µM 1.18E-5 endomembrane recycling 88 8.60E-6
9 SGTC_3194 9111903 4.23 49.5 µM 1.56E-5 18 1.15E-5
10 SGTC_827 3253-0474 4.15 693.0 µM 2.22E-5 15 1.64E-5
11 SGTC_3136 9095672 4.12 49.5 µM 2.61E-5 23 1.93E-5
12 SGTC_1181 1222-0449 4.07 220.0 µM 3.09E-5 24 2.31E-5
13 SGTC_3234 9132280 3.92 49.5 µM 5.79E-5 19 4.39E-5
14 SGTC_2342 7990637 3.71 118.9 µM 1.36E-4 RNA processing & uracil transport 44 1.05E-4
15 SGTC_825 1988-2224 3.70 89.6 µM 1.38E-4 cell wall signaling 42 1.07E-4
16 SGTC_280 3448-3607 3.67 3.0 µM 1.56E-4 60S ribosome export 15 1.21E-4
17 SGTC_3229 9132204 3.54 49.5 µM 2.51E-4 8 1.97E-4
18 SGTC_87 0100-0052 3.54 35.5 µM 2.53E-4 146 1.99E-4
19 SGTC_3207 9114163 3.48 49.5 µM 3.11E-4 50 2.46E-4
20 SGTC_2297 7971671 3.42 152.3 µM 3.98E-4 RNA processing & uracil transport 49 3.17E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.223 4.57E-39 YML062C MFT1 hom Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
0.168 1.40E-22 YGR122C-A_d YGR122C-A_d hom Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W
0.144 4.86E-17 YMR015C ERG5 hom C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
0.141 1.95E-16 YDL136W RPL35B hom Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication
0.141 2.02E-16 YDR101C ARX1 hom Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex
0.132 1.95E-14 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.130 4.13E-14 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.128 1.20E-13 YNL032W SIW14 hom Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm
0.127 1.35E-13 YJR088C EMC2 hom Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35
0.127 1.95E-13 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.125 4.32E-13 YBR290W BSD2 hom Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification
0.124 5.60E-13 YLL045C RPL8B hom Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication
0.124 5.65E-13 YDL052C SLC1 hom 1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes
0.122 1.33E-12 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.118 7.69E-12 YLR184W_d YLR184W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data