HIPHOP chemogenomics database

YNL006W / LST8

Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat

Zygosity: Heterozygous strain

fixedexpanded
Profile for YNL006W / LST8

Click on Significant Values for Screen Details

Top fitness defect scores for YNL006W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_2249	haloperidol	6.56	116.4 µM	2.68E-13	ergosterol depletion effects on membrane	15	2.71E-11	0.75
2	SGTC_593	1203-0218	5.44	61.7 µM	1.03E-9		1	2.63E-8	0.92
3	SGTC_538	2922-0838	5.28	53.3 µM	2.99E-9	Golgi	2	6.38E-8	1.06
4	SGTC_913	3487-1245	5.00	167.0 µM	1.81E-8		5	2.86E-7	0.10
5	SGTC_910	3454-3003	4.99	490.0 µM	1.93E-8		4	3.02E-7	1.15
6	SGTC_2967	9089538	4.92	49.9 µM	2.91E-8		4	4.25E-7	0.02
7	SGTC_908	3448-7491	4.81	47.7 µM	5.77E-8	mitochondrial stress	5	7.53E-7	0.30
8	SGTC_2241	6772625	4.63	37.8 µM	1.64E-7		5	1.80E-6	0.47
9	SGTC_173	1273-0061	4.49	2.7 µM	3.66E-7	mitochondrial stress	18	3.52E-6	0.11
10	SGTC_2443	5493264	4.45	126.7 µM	4.66E-7		3	4.30E-6	0.35
11	SGTC_548	1436-0017	4.34	110.0 µM	8.77E-7	TSC3-RPN4	4	7.29E-6	0.33
12	SGTC_2640	pentamidine	3.98	10.4 µM	5.62E-6	mitochondrial processes	15	3.44E-5	0.01
13	SGTC_3318	9139014	3.93	38.6 µM	7.13E-6		10	4.19E-5	0.03
14	SGTC_2246	7293507	3.81	91.6 µM	1.31E-5		11	6.94E-5	0.04
15	SGTC_662	k832-1615	3.68	104.0 µM	2.40E-5		6	1.16E-4	0.02
16	SGTC_2401	5128683	3.58	200.0 µM	3.91E-5		13	1.74E-4	0.05
17	SGTC_12	0849-0099	3.56	9.3 µM	4.24E-5		8	1.86E-4	0.22
18	SGTC_524	r073-0010	3.46	27.8 µM	6.70E-5		22	2.72E-4	0.05
19	SGTC_2849	9019321	3.45	13.0 µM	7.05E-5		11	2.84E-4	0.11
20	SGTC_3334	9142812	3.40	21.9 µM	8.46E-5		10	3.31E-4	0.03

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.116	1.40E-11	YGL068W	MNP1	het	Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth
0.111	1.15E-10	YPL085W	SEC16	het	COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p
0.111	1.29E-10	YKL037W	AIM26	hom	Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT
0.109	2.36E-10	YMR043W	MCM1	het	Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes
0.107	4.92E-10	YPL189W	GUP2	hom	Probable membrane protein with a possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; Gup1p homolog
0.103	2.46E-9	YEL025C_p	YEL025C_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
0.093	7.29E-8	YOL028C	YAP7	hom	Putative basic leucine zipper (bZIP) transcription factor; YAP7 has a paralog, YAP5, that arose from the whole genome duplication
0.092	1.06E-7	YDR499W	LCD1	het	Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress
0.091	1.14E-7	YJL159W	HSP150	hom	O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.090	1.63E-7	YIL109C	SEC24	het	Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p
0.090	2.01E-7	YML121W	GTR1	hom	Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.089	2.80E-7	YLR283W_p	YLR283W_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene
0.088	3.43E-7	YNL044W	YIP3	hom	Protein localized to COPII vesicles, proposed to be involved in ER to Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p
0.086	5.56E-7	YLR368W	MDM30	hom	F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains
0.085	7.73E-7	YPR099C_d	YPR099C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W