YNL026W / SAM50

Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL026W / SAM50

Click on Significant Values for Screen Details ID:SGTC_103|Compound:0844-0104|FD-Score:4.27|P-value:3.80E-6 ID:SGTC_149|Compound:3970-0822|FD-Score:3.08|P-value:6.42E-4 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:3.93|P-value:1.92E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.03|P-value:1.01E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:5.39|P-value:7.92E-9 ID:SGTC_366|Compound:0181-0206|FD-Score:3.38|P-value:1.99E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.26|P-value:2.76E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:4.13|P-value:7.55E-6 ID:SGTC_384|Compound:k007-0144|FD-Score:3.46|P-value:1.42E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:4.69|P-value:4.33E-7 ID:SGTC_575|Compound:k072-0246|FD-Score:3.15|P-value:4.79E-4 ID:SGTC_593|Compound:1203-0218|FD-Score:-3.09|P-value:5.44E-4 ID:SGTC_594|Compound:1487-1584|FD-Score:3.33|P-value:2.45E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:-3.61|P-value:6.69E-5 ID:SGTC_616|Compound:4092-0450|FD-Score:3.51|P-value:1.20E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:3.82|P-value:3.18E-5 ID:SGTC_663|Compound:1486-0733|FD-Score:4.37|P-value:2.26E-6 ID:SGTC_733|Compound:4069-0007|FD-Score:3.97|P-value:1.55E-5 ID:SGTC_893|Compound:0958-0369|FD-Score:-8.23|P-value:2.01E-18 ID:SGTC_960|Compound:1222-0186|FD-Score:3.37|P-value:2.08E-4 ID:SGTC_971|Compound:1315-0384|FD-Score:3.05|P-value:6.99E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:6.67|P-value:1.21E-12 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.86|P-value:2.23E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.14|P-value:5.04E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:4.74|P-value:3.24E-7 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.33|P-value:2.43E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:3.56|P-value:9.51E-5 ID:SGTC_1443|Compound:4112-3303|FD-Score:3.50|P-value:1.24E-4 ID:SGTC_1602|Compound:st001878|FD-Score:3.62|P-value:7.47E-5 ID:SGTC_1658|Compound:st012944|FD-Score:3.29|P-value:2.82E-4 ID:SGTC_1675|Compound:st018485|FD-Score:3.26|P-value:3.19E-4 ID:SGTC_1676|Compound:st018489|FD-Score:3.55|P-value:9.91E-5 ID:SGTC_1799|Compound:st045822|FD-Score:-3.46|P-value:1.28E-4 ID:SGTC_1831|Compound:st055328|FD-Score:4.81|P-value:2.22E-7 ID:SGTC_2380|Compound:9035439|FD-Score:-3.13|P-value:4.59E-4 ID:SGTC_2385|Compound:9074692|FD-Score:3.98|P-value:1.49E-5 ID:SGTC_2440|Compound:5628896|FD-Score:3.88|P-value:2.43E-5 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:4.34|P-value:2.63E-6 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.74|P-value:4.49E-5 ID:SGTC_2583|Compound:asiatic acid|FD-Score:4.68|P-value:4.68E-7 ID:SGTC_2620|Compound:irigenin|FD-Score:-6.65|P-value:1.15E-12 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-5.14|P-value:2.78E-8 ID:SGTC_2629|Compound:bisabolol|FD-Score:-4.00|P-value:1.19E-5 ID:SGTC_2630|Compound:antimycin a|FD-Score:-4.17|P-value:5.16E-6 ID:SGTC_2683|Compound:piperine|FD-Score:-3.17|P-value:3.93E-4 ID:SGTC_2794|Compound:7785382|FD-Score:-3.29|P-value:2.48E-4 ID:SGTC_2918|Compound:7977171|FD-Score:3.10|P-value:5.81E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-3.17|P-value:3.99E-4 ID:SGTC_2944|Compound:9067346|FD-Score:2.96|P-value:9.61E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-3.29|P-value:2.46E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-3.39|P-value:1.65E-4 ID:SGTC_2980|Compound:9014342|FD-Score:3.30|P-value:2.79E-4 ID:SGTC_3107|Compound:9121808|FD-Score:3.21|P-value:3.92E-4 ID:SGTC_3116|Compound:9124943|FD-Score:3.03|P-value:7.61E-4 ID:SGTC_3313|Compound:9133968|FD-Score:-4.17|P-value:5.25E-6 ID:SGTC_103|Compound:0844-0104|FD-Score:4.27|P-value:3.80E-6 ID:SGTC_149|Compound:3970-0822|FD-Score:3.08|P-value:6.42E-4 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:3.93|P-value:1.92E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.03|P-value:1.01E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:5.39|P-value:7.92E-9 ID:SGTC_366|Compound:0181-0206|FD-Score:3.38|P-value:1.99E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.26|P-value:2.76E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:4.13|P-value:7.55E-6 ID:SGTC_384|Compound:k007-0144|FD-Score:3.46|P-value:1.42E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:4.69|P-value:4.33E-7 ID:SGTC_575|Compound:k072-0246|FD-Score:3.15|P-value:4.79E-4 ID:SGTC_593|Compound:1203-0218|FD-Score:-3.09|P-value:5.44E-4 ID:SGTC_594|Compound:1487-1584|FD-Score:3.33|P-value:2.45E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:-3.61|P-value:6.69E-5 ID:SGTC_616|Compound:4092-0450|FD-Score:3.51|P-value:1.20E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:3.82|P-value:3.18E-5 ID:SGTC_663|Compound:1486-0733|FD-Score:4.37|P-value:2.26E-6 ID:SGTC_733|Compound:4069-0007|FD-Score:3.97|P-value:1.55E-5 ID:SGTC_893|Compound:0958-0369|FD-Score:-8.23|P-value:2.01E-18 ID:SGTC_960|Compound:1222-0186|FD-Score:3.37|P-value:2.08E-4 ID:SGTC_971|Compound:1315-0384|FD-Score:3.05|P-value:6.99E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:6.67|P-value:1.21E-12 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.86|P-value:2.23E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.14|P-value:5.04E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:4.74|P-value:3.24E-7 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.33|P-value:2.43E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:3.56|P-value:9.51E-5 ID:SGTC_1443|Compound:4112-3303|FD-Score:3.50|P-value:1.24E-4 ID:SGTC_1602|Compound:st001878|FD-Score:3.62|P-value:7.47E-5 ID:SGTC_1658|Compound:st012944|FD-Score:3.29|P-value:2.82E-4 ID:SGTC_1675|Compound:st018485|FD-Score:3.26|P-value:3.19E-4 ID:SGTC_1676|Compound:st018489|FD-Score:3.55|P-value:9.91E-5 ID:SGTC_1799|Compound:st045822|FD-Score:-3.46|P-value:1.28E-4 ID:SGTC_1831|Compound:st055328|FD-Score:4.81|P-value:2.22E-7 ID:SGTC_2380|Compound:9035439|FD-Score:-3.13|P-value:4.59E-4 ID:SGTC_2385|Compound:9074692|FD-Score:3.98|P-value:1.49E-5 ID:SGTC_2440|Compound:5628896|FD-Score:3.88|P-value:2.43E-5 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:4.34|P-value:2.63E-6 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.74|P-value:4.49E-5 ID:SGTC_2583|Compound:asiatic acid|FD-Score:4.68|P-value:4.68E-7 ID:SGTC_2620|Compound:irigenin|FD-Score:-6.65|P-value:1.15E-12 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-5.14|P-value:2.78E-8 ID:SGTC_2629|Compound:bisabolol|FD-Score:-4.00|P-value:1.19E-5 ID:SGTC_2630|Compound:antimycin a|FD-Score:-4.17|P-value:5.16E-6 ID:SGTC_2683|Compound:piperine|FD-Score:-3.17|P-value:3.93E-4 ID:SGTC_2794|Compound:7785382|FD-Score:-3.29|P-value:2.48E-4 ID:SGTC_2918|Compound:7977171|FD-Score:3.10|P-value:5.81E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-3.17|P-value:3.99E-4 ID:SGTC_2944|Compound:9067346|FD-Score:2.96|P-value:9.61E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-3.29|P-value:2.46E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-3.39|P-value:1.65E-4 ID:SGTC_2980|Compound:9014342|FD-Score:3.30|P-value:2.79E-4 ID:SGTC_3107|Compound:9121808|FD-Score:3.21|P-value:3.92E-4 ID:SGTC_3116|Compound:9124943|FD-Score:3.03|P-value:7.61E-4 ID:SGTC_3313|Compound:9133968|FD-Score:-4.17|P-value:5.25E-6

Top fitness defect scores for YNL026W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1026 k007-1105 6.67 30.6 µM 1.21E-12 ERAD & cell cycle 10 1.24E-11 0.14
2 SGTC_352 k048-0007 5.39 5.1 µM 7.92E-9 ERAD & cell cycle 4 3.59E-8 0.03
3 SGTC_1831 st055328 4.81 12.0 µM 2.22E-7 heme biosynthesis & mitochondrial translocase 8 7.41E-7 0.16
4 SGTC_1165 k072-0275 4.74 30.3 µM 3.24E-7 4 1.05E-6 0.17
5 SGTC_426 0988-0037 4.69 14.6 µM 4.33E-7 ERAD & cell cycle 5 1.36E-6 0.03
6 SGTC_2583 asiatic acid 4.68 100.0 µM 4.68E-7 4 1.46E-6 0.21
7 SGTC_663 1486-0733 4.37 13.9 µM 2.26E-6 cell wall signaling 7 6.10E-6 0.44
8 SGTC_2541 muurolladie-3-one 4.34 83.1 µM 2.63E-6 excess fatty acid 17 7.01E-6 0.29
9 SGTC_103 0844-0104 4.27 51.8 µM 3.80E-6 ERAD & cell cycle 45 9.79E-6 0.14
10 SGTC_373 0844-0104 4.13 25.9 µM 7.55E-6 ERAD & cell cycle 9 1.83E-5 0.02
11 SGTC_2385 9074692 3.98 200.0 µM 1.49E-5 4 3.40E-5 0.02
12 SGTC_733 4069-0007 3.97 56.1 µM 1.55E-5 ERAD & cell cycle 20 3.53E-5 0.05
13 SGTC_226 1,3-diallylurea 3.93 8.3 mM 1.92E-5 12 4.28E-5 0.35
14 SGTC_2440 5628896 3.88 157.8 µM 2.43E-5 7 5.31E-5 0.26
15 SGTC_659 k018-0002 3.82 107.0 µM 3.18E-5 7 6.78E-5 0.02
16 SGTC_2564 betulinic acid 3.74 100.0 µM 4.49E-5 10 9.28E-5 0.21
17 SGTC_1602 st001878 3.62 87.6 µM 7.47E-5 10 1.48E-4 0.17
18 SGTC_1425 4048-4433 3.56 7.3 µM 9.51E-5 copper-dependent oxidative stress 8 1.84E-4 0.21
19 SGTC_1676 st018489 3.55 23.4 µM 9.91E-5 60S ribosome export 8 1.91E-4 0.13
20 SGTC_616 4092-0450 3.51 108.0 µM 1.20E-4 5 2.27E-4 0.10

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.338 1.75E-90 YOL002C IZH2 hom Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
0.336 1.59E-89 YIL030C SSM4 hom Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.294 3.75E-68 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.283 1.05E-62 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.251 2.03E-49 YMR052C-A_d YMR052C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.248 3.50E-48 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.226 2.88E-40 YDR199W_d YDR199W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity
0.224 2.83E-39 YLR065C ENV10 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene
0.207 6.11E-34 YMR029C FAR8 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.207 8.59E-34 YMR052W FAR3 hom Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
0.205 2.66E-33 YDL100C GET3 hom Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
0.195 4.90E-30 YML014W TRM9 hom tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
0.181 3.81E-26 YMR258C ROY1 hom GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p
0.169 5.37E-23 YGL055W OLE1 het Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
0.167 1.59E-22 YKR014C YPT52 hom Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress