YNL053W / MSG5

Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL053W / MSG5

Click on Significant Values for Screen Details ID:SGTC_132|Compound:0417-1715|FD-Score:5.00|P-value:6.86E-7 ID:SGTC_140|Compound:0062-0056|FD-Score:-4.96|P-value:7.35E-7 ID:SGTC_354|Compound:2783-3500|FD-Score:3.67|P-value:1.96E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:3.85|P-value:9.86E-5 ID:SGTC_493|Compound:zm226600|FD-Score:3.34|P-value:6.40E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:8.38|P-value:2.70E-16 ID:SGTC_572|Compound:0960-0087|FD-Score:3.97|P-value:6.42E-5 ID:SGTC_791|Compound:1348-1292|FD-Score:-3.34|P-value:5.93E-4 ID:SGTC_825|Compound:1988-2224|FD-Score:3.32|P-value:6.65E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:4.29|P-value:1.72E-5 ID:SGTC_1000|Compound:4048-0884|FD-Score:3.54|P-value:3.17E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:-3.74|P-value:1.43E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:-3.88|P-value:8.38E-5 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.21|P-value:9.58E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.24|P-value:8.96E-4 ID:SGTC_1486|Compound:3970-0793|FD-Score:4.22|P-value:2.27E-5 ID:SGTC_1603|Compound:st002045|FD-Score:3.47|P-value:4.03E-4 ID:SGTC_1745|Compound:st081495|FD-Score:3.63|P-value:2.24E-4 ID:SGTC_1800|Compound:st050079|FD-Score:4.14|P-value:3.12E-5 ID:SGTC_1825|Compound:st052143|FD-Score:-3.24|P-value:8.27E-4 ID:SGTC_1837|Compound:st055472|FD-Score:3.79|P-value:1.25E-4 ID:SGTC_1842|Compound:st055362|FD-Score:3.61|P-value:2.43E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.57|P-value:2.89E-4 ID:SGTC_1961|Compound:st076550|FD-Score:3.29|P-value:7.39E-4 ID:SGTC_1963|Compound:st076565|FD-Score:3.34|P-value:6.32E-4 ID:SGTC_1967|Compound:st070177|FD-Score:-3.23|P-value:8.54E-4 ID:SGTC_1971|Compound:st069429|FD-Score:5.71|P-value:1.73E-8 ID:SGTC_1973|Compound:st070275|FD-Score:3.74|P-value:1.50E-4 ID:SGTC_2119|Compound:5528445|FD-Score:3.77|P-value:1.38E-4 ID:SGTC_2370|Compound:9071495|FD-Score:-3.89|P-value:8.02E-5 ID:SGTC_2384|Compound:9036245|FD-Score:-3.72|P-value:1.53E-4 ID:SGTC_2438|Compound:5259590|FD-Score:4.01|P-value:5.35E-5 ID:SGTC_2442|Compound:5158906|FD-Score:3.28|P-value:7.62E-4 ID:SGTC_2461|Compound:5755302|FD-Score:-3.34|P-value:5.89E-4 ID:SGTC_2475|Compound:5812728|FD-Score:-4.21|P-value:2.23E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.63|P-value:3.53E-6 ID:SGTC_2584|Compound:androsterone|FD-Score:3.54|P-value:3.17E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.27|P-value:8.07E-4 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:-4.41|P-value:9.20E-6 ID:SGTC_2649|Compound:anisomycin|FD-Score:-3.76|P-value:1.33E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.94|P-value:8.36E-7 ID:SGTC_2676|Compound:harmalol|FD-Score:5.63|P-value:2.58E-8 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.38|P-value:5.51E-4 ID:SGTC_2833|Compound:9001315|FD-Score:3.35|P-value:6.17E-4 ID:SGTC_2928|Compound:9011484|FD-Score:3.49|P-value:3.79E-4 ID:SGTC_2985|Compound:9022375|FD-Score:-3.77|P-value:1.25E-4 ID:SGTC_2989|Compound:9073649|FD-Score:3.52|P-value:3.41E-4 ID:SGTC_3083|Compound:9117465|FD-Score:-4.64|P-value:3.41E-6 ID:SGTC_3153|Compound:9100258|FD-Score:-4.86|P-value:1.20E-6 ID:SGTC_132|Compound:0417-1715|FD-Score:5.00|P-value:6.86E-7 ID:SGTC_140|Compound:0062-0056|FD-Score:-4.96|P-value:7.35E-7 ID:SGTC_354|Compound:2783-3500|FD-Score:3.67|P-value:1.96E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:3.85|P-value:9.86E-5 ID:SGTC_493|Compound:zm226600|FD-Score:3.34|P-value:6.40E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:8.38|P-value:2.70E-16 ID:SGTC_572|Compound:0960-0087|FD-Score:3.97|P-value:6.42E-5 ID:SGTC_791|Compound:1348-1292|FD-Score:-3.34|P-value:5.93E-4 ID:SGTC_825|Compound:1988-2224|FD-Score:3.32|P-value:6.65E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:4.29|P-value:1.72E-5 ID:SGTC_1000|Compound:4048-0884|FD-Score:3.54|P-value:3.17E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:-3.74|P-value:1.43E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:-3.88|P-value:8.38E-5 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.21|P-value:9.58E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.24|P-value:8.96E-4 ID:SGTC_1486|Compound:3970-0793|FD-Score:4.22|P-value:2.27E-5 ID:SGTC_1603|Compound:st002045|FD-Score:3.47|P-value:4.03E-4 ID:SGTC_1745|Compound:st081495|FD-Score:3.63|P-value:2.24E-4 ID:SGTC_1800|Compound:st050079|FD-Score:4.14|P-value:3.12E-5 ID:SGTC_1825|Compound:st052143|FD-Score:-3.24|P-value:8.27E-4 ID:SGTC_1837|Compound:st055472|FD-Score:3.79|P-value:1.25E-4 ID:SGTC_1842|Compound:st055362|FD-Score:3.61|P-value:2.43E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:3.57|P-value:2.89E-4 ID:SGTC_1961|Compound:st076550|FD-Score:3.29|P-value:7.39E-4 ID:SGTC_1963|Compound:st076565|FD-Score:3.34|P-value:6.32E-4 ID:SGTC_1967|Compound:st070177|FD-Score:-3.23|P-value:8.54E-4 ID:SGTC_1971|Compound:st069429|FD-Score:5.71|P-value:1.73E-8 ID:SGTC_1973|Compound:st070275|FD-Score:3.74|P-value:1.50E-4 ID:SGTC_2119|Compound:5528445|FD-Score:3.77|P-value:1.38E-4 ID:SGTC_2370|Compound:9071495|FD-Score:-3.89|P-value:8.02E-5 ID:SGTC_2384|Compound:9036245|FD-Score:-3.72|P-value:1.53E-4 ID:SGTC_2438|Compound:5259590|FD-Score:4.01|P-value:5.35E-5 ID:SGTC_2442|Compound:5158906|FD-Score:3.28|P-value:7.62E-4 ID:SGTC_2461|Compound:5755302|FD-Score:-3.34|P-value:5.89E-4 ID:SGTC_2475|Compound:5812728|FD-Score:-4.21|P-value:2.23E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.63|P-value:3.53E-6 ID:SGTC_2584|Compound:androsterone|FD-Score:3.54|P-value:3.17E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.27|P-value:8.07E-4 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:-4.41|P-value:9.20E-6 ID:SGTC_2649|Compound:anisomycin|FD-Score:-3.76|P-value:1.33E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:-4.94|P-value:8.36E-7 ID:SGTC_2676|Compound:harmalol|FD-Score:5.63|P-value:2.58E-8 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.38|P-value:5.51E-4 ID:SGTC_2833|Compound:9001315|FD-Score:3.35|P-value:6.17E-4 ID:SGTC_2928|Compound:9011484|FD-Score:3.49|P-value:3.79E-4 ID:SGTC_2985|Compound:9022375|FD-Score:-3.77|P-value:1.25E-4 ID:SGTC_2989|Compound:9073649|FD-Score:3.52|P-value:3.41E-4 ID:SGTC_3083|Compound:9117465|FD-Score:-4.64|P-value:3.41E-6 ID:SGTC_3153|Compound:9100258|FD-Score:-4.86|P-value:1.20E-6

Top fitness defect scores for YNL053W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_514 4466-0038 8.38 2.6 nM 2.70E-16 cell wall 31 2.69E-17
2 SGTC_1971 st069429 5.71 63.5 µM 1.73E-8 60S ribosome export 2 5.73E-9
3 SGTC_2676 harmalol 5.63 100.0 µM 2.58E-8 mitochondrial processes 1 8.76E-9
4 SGTC_132 0417-1715 5.00 25.8 µM 6.86E-7 RPP1 & pyrimidine depletion 8 2.91E-7
5 SGTC_999 4022-0066 4.29 22.7 µM 1.72E-5 Golgi 31 9.01E-6
6 SGTC_1486 3970-0793 4.22 4.5 µM 2.27E-5 6 1.21E-5
7 SGTC_1800 st050079 4.14 29.1 µM 3.12E-5 NEO1-PIK1 111 1.70E-5
8 SGTC_2438 5259590 4.01 200.0 µM 5.35E-5 17 3.02E-5
9 SGTC_572 0960-0087 3.97 17.7 µM 6.42E-5 27 3.67E-5
10 SGTC_367 0335-0881 3.85 7.1 µM 9.86E-5 ubiquinone biosynthesis & proteasome 84 5.79E-5
11 SGTC_1837 st055472 3.79 66.5 µM 1.25E-4 23 7.43E-5
12 SGTC_2119 5528445 3.77 120.6 µM 1.38E-4 25 8.31E-5
13 SGTC_1973 st070275 3.74 50.2 µM 1.50E-4 13 9.06E-5
14 SGTC_354 2783-3500 3.67 4.9 µM 1.96E-4 cell wall 55 1.20E-4
15 SGTC_1745 st081495 3.63 74.0 µM 2.24E-4 RNA processing & uracil transport 84 1.39E-4
16 SGTC_1842 st055362 3.61 61.2 µM 2.43E-4 21 1.52E-4
17 SGTC_1918 oxolan-2-ylmethyl 3-bromobenzoate 3.57 70.1 µM 2.89E-4 42 1.82E-4
18 SGTC_1000 4048-0884 3.54 8.7 µM 3.17E-4 copper-dependent oxidative stress 43 2.01E-4
19 SGTC_2584 androsterone 3.54 48.7 µM 3.17E-4 cell wall 155 2.01E-4
20 SGTC_2989 9073649 3.52 71.4 µM 3.41E-4 58 2.17E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.221 2.85E-38 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.172 1.15E-23 YMR036C MIH1 hom Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.168 1.23E-22 YDR121W DPB4 hom Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
0.158 2.85E-20 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.150 2.71E-18 YMR106C YKU80 hom Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair
0.144 4.05E-17 YLR102C APC9 hom Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.139 6.68E-16 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.130 4.95E-14 YDR170C SEC7 het Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles
0.128 1.11E-13 YDR389W SAC7 hom GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate
0.125 3.71E-13 YOR015W_d YOR015W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.123 7.59E-13 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.120 3.25E-12 YML119W_p YML119W_p hom Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate
0.118 5.71E-12 YDR348C PAL1 hom Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.112 7.35E-11 YPL017C IRC15 hom Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci
0.112 8.72E-11 YPR143W RRP15 het Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs