HIPHOP chemogenomics database

YNL061W / NOP2

Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles

Zygosity: Heterozygous strain

fixedexpanded
Profile for YNL061W / NOP2

Click on Significant Values for Screen Details

Top fitness defect scores for YNL061W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_1714	st033236	7.47	13.6 µM	1.10E-12		1	4.12E-14	1.88
2	SGTC_610	4215-0184	6.99	155.0 µM	2.50E-11	exosome	8	1.40E-12	0.23
3	SGTC_1074	carmofur	5.97	3.8 µM	9.79E-9	exosome	15	1.17E-9	0.07
4	SGTC_1719	st032227	5.91	49.2 µM	1.37E-8		3	1.71E-9	0.74
5	SGTC_879	0831-1030	5.77	162.0 µM	2.94E-8		5	4.05E-9	0.37
6	SGTC_1698	5-fluorocytosine	5.62	1.1 µM	6.45E-8	exosome	11	9.82E-9	0.04
7	SGTC_1713	st033146	5.54	12.8 µM	9.34E-8		2	1.49E-8	0.37
8	SGTC_3294	9122953	5.34	26.3 µM	2.56E-7	tubulin folding & SWR complex	1	4.64E-8	0.89
9	SGTC_989	1501-0895	5.22	10.3 µM	4.48E-7		1	8.72E-8	1.12
10	SGTC_675	1309-0189	5.20	238.0 µM	5.07E-7		2	1.00E-7	1.04
11	SGTC_653	0203-0016	5.05	55.2 µM	1.04E-6		2	2.26E-7	0.78
12	SGTC_242	5hr heat shock (37°C) + 5-fluorouracil	4.93	4.6 µM	1.74E-6	heat shock/prefoldin	14	4.03E-7	0.05
13	SGTC_2838	9002687	4.83	71.4 µM	2.74E-6	fatty acid desaturase (OLE1)	9	6.69E-7	0.31
14	SGTC_2342	7990637	4.69	118.9 µM	5.17E-6	RNA processing & uracil transport	4	1.37E-6	0.45
15	SGTC_1677	st019204	4.65	53.9 µM	6.03E-6		3	1.63E-6	0.02
16	SGTC_640	3381-0960	4.62	37.1 µM	6.96E-6		3	1.91E-6	0.27
17	SGTC_641	4092-0595	4.50	30.0 µM	1.16E-5		5	3.41E-6	0.04
18	SGTC_261	5-fluorouridine	4.47	15.4 µM	1.31E-5	exosome	10	3.91E-6	0.14
19	SGTC_1992	st073643	4.35	23.3 µM	2.12E-5	mitochondrial processes	7	6.71E-6	0.13
20	SGTC_423	5-fluorouracil	4.31	457.0 µM	2.52E-5	exosome	24	8.12E-6	0.05

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.292	1.08E-66	YOL021C	DIS3	het	Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
0.277	3.11E-60	YGR195W	SKI6	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.256	4.17E-51	YDL111C	RRP42	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
0.240	4.37E-45	YGR095C	RRP46	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
0.237	5.24E-44	YGR158C	MTR3	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
0.217	7.28E-37	YPL044C_d	YPL044C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.209	2.53E-34	YDR280W	RRP45	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
0.203	1.38E-32	YPL043W	NOP4	het	Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
0.194	1.37E-29	YOR206W	NOC2	het	Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
0.193	1.79E-29	YLR009W	RLP24	het	Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
0.182	3.05E-26	YKL172W	EBP2	het	Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.179	1.52E-25	YPR142C_d	YPR142C_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
0.171	1.80E-23	YNL232W	CSL4	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
0.162	3.98E-21	YPR143W	RRP15	het	Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
0.150	3.03E-18	YHR069C	RRP4	het	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)