YNL061W / NOP2

Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL061W / NOP2

Click on Significant Values for Screen Details ID:SGTC_66|Compound:0109-0187|FD-Score:3.37|P-value:7.72E-4 ID:SGTC_88|Compound:0180-0332|FD-Score:4.08|P-value:6.30E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:3.45|P-value:5.84E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:4.93|P-value:1.74E-6 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:4.47|P-value:1.31E-5 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:4.31|P-value:2.52E-5 ID:SGTC_490|Compound:phenamil|FD-Score:-4.28|P-value:2.83E-5 ID:SGTC_493|Compound:zm226600|FD-Score:3.72|P-value:2.36E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:6.99|P-value:2.50E-11 ID:SGTC_640|Compound:3381-0960|FD-Score:4.62|P-value:6.96E-6 ID:SGTC_641|Compound:4092-0595|FD-Score:4.50|P-value:1.16E-5 ID:SGTC_652|Compound:0437-0092|FD-Score:3.72|P-value:2.37E-4 ID:SGTC_653|Compound:0203-0016|FD-Score:5.05|P-value:1.04E-6 ID:SGTC_675|Compound:1309-0189|FD-Score:5.20|P-value:5.07E-7 ID:SGTC_800|Compound:3448-7362|FD-Score:3.96|P-value:9.72E-5 ID:SGTC_879|Compound:0831-1030|FD-Score:5.77|P-value:2.94E-8 ID:SGTC_989|Compound:1501-0895|FD-Score:5.22|P-value:4.48E-7 ID:SGTC_1074|Compound:carmofur|FD-Score:5.97|P-value:9.79E-9 ID:SGTC_1269|Compound:0831-0796|FD-Score:-4.20|P-value:3.90E-5 ID:SGTC_1433|Compound:4060-0023|FD-Score:3.78|P-value:1.90E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.32|P-value:8.87E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:-3.44|P-value:6.14E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.59|P-value:3.68E-4 ID:SGTC_1619|Compound:st003326|FD-Score:-3.48|P-value:5.27E-4 ID:SGTC_1649|Compound:st011737|FD-Score:-3.92|P-value:1.14E-4 ID:SGTC_1677|Compound:st019204|FD-Score:4.65|P-value:6.03E-6 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:5.62|P-value:6.45E-8 ID:SGTC_1713|Compound:st033146|FD-Score:5.54|P-value:9.34E-8 ID:SGTC_1714|Compound:st033236|FD-Score:7.47|P-value:1.10E-12 ID:SGTC_1719|Compound:st032227|FD-Score:5.91|P-value:1.37E-8 ID:SGTC_1786|Compound:berberine|FD-Score:-3.76|P-value:2.06E-4 ID:SGTC_1941|Compound:st076221|FD-Score:-4.60|P-value:7.45E-6 ID:SGTC_1945|Compound:st075991|FD-Score:3.62|P-value:3.27E-4 ID:SGTC_1992|Compound:st073643|FD-Score:4.35|P-value:2.12E-5 ID:SGTC_2066|Compound:5222451|FD-Score:-3.35|P-value:8.24E-4 ID:SGTC_2072|Compound:5231554|FD-Score:4.27|P-value:2.98E-5 ID:SGTC_2111|Compound:5486399|FD-Score:-3.52|P-value:4.59E-4 ID:SGTC_2124|Compound:5246320|FD-Score:-3.46|P-value:5.75E-4 ID:SGTC_2180|Compound:6301957|FD-Score:-3.60|P-value:3.51E-4 ID:SGTC_2246|Compound:7293507|FD-Score:-3.90|P-value:1.21E-4 ID:SGTC_2323|Compound:6148992|FD-Score:3.71|P-value:2.39E-4 ID:SGTC_2336|Compound:9001819|FD-Score:-4.00|P-value:8.43E-5 ID:SGTC_2342|Compound:7990637|FD-Score:4.69|P-value:5.17E-6 ID:SGTC_2344|Compound:7990960|FD-Score:-3.32|P-value:9.08E-4 ID:SGTC_2348|Compound:9022543|FD-Score:-3.32|P-value:9.06E-4 ID:SGTC_2350|Compound:9023895|FD-Score:-6.31|P-value:1.50E-9 ID:SGTC_2423|Compound:st077119|FD-Score:3.93|P-value:1.09E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-5.08|P-value:8.98E-7 ID:SGTC_2739|Compound:tacrine|FD-Score:-3.95|P-value:9.99E-5 ID:SGTC_2756|Compound:carbadox|FD-Score:-3.58|P-value:3.78E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:-3.34|P-value:8.30E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-4.99|P-value:1.32E-6 ID:SGTC_2825|Compound:7998094|FD-Score:3.30|P-value:9.69E-4 ID:SGTC_2838|Compound:9002687|FD-Score:4.83|P-value:2.74E-6 ID:SGTC_2930|Compound:9013230|FD-Score:-3.51|P-value:4.82E-4 ID:SGTC_3071|Compound:9117417|FD-Score:3.89|P-value:1.27E-4 ID:SGTC_3072|Compound:9116419|FD-Score:-5.17|P-value:5.77E-7 ID:SGTC_3222|Compound:9128301|FD-Score:-4.71|P-value:4.73E-6 ID:SGTC_3249|Compound:9135183|FD-Score:-4.32|P-value:2.40E-5 ID:SGTC_3287|Compound:9117173|FD-Score:-4.35|P-value:2.15E-5 ID:SGTC_3294|Compound:9122953|FD-Score:5.34|P-value:2.56E-7 ID:SGTC_3334|Compound:9142812|FD-Score:3.31|P-value:9.21E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:3.37|P-value:7.72E-4 ID:SGTC_88|Compound:0180-0332|FD-Score:4.08|P-value:6.30E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:3.45|P-value:5.84E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:4.93|P-value:1.74E-6 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:4.47|P-value:1.31E-5 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:4.31|P-value:2.52E-5 ID:SGTC_490|Compound:phenamil|FD-Score:-4.28|P-value:2.83E-5 ID:SGTC_493|Compound:zm226600|FD-Score:3.72|P-value:2.36E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:6.99|P-value:2.50E-11 ID:SGTC_640|Compound:3381-0960|FD-Score:4.62|P-value:6.96E-6 ID:SGTC_641|Compound:4092-0595|FD-Score:4.50|P-value:1.16E-5 ID:SGTC_652|Compound:0437-0092|FD-Score:3.72|P-value:2.37E-4 ID:SGTC_653|Compound:0203-0016|FD-Score:5.05|P-value:1.04E-6 ID:SGTC_675|Compound:1309-0189|FD-Score:5.20|P-value:5.07E-7 ID:SGTC_800|Compound:3448-7362|FD-Score:3.96|P-value:9.72E-5 ID:SGTC_879|Compound:0831-1030|FD-Score:5.77|P-value:2.94E-8 ID:SGTC_989|Compound:1501-0895|FD-Score:5.22|P-value:4.48E-7 ID:SGTC_1074|Compound:carmofur|FD-Score:5.97|P-value:9.79E-9 ID:SGTC_1269|Compound:0831-0796|FD-Score:-4.20|P-value:3.90E-5 ID:SGTC_1433|Compound:4060-0023|FD-Score:3.78|P-value:1.90E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.32|P-value:8.87E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:-3.44|P-value:6.14E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.59|P-value:3.68E-4 ID:SGTC_1619|Compound:st003326|FD-Score:-3.48|P-value:5.27E-4 ID:SGTC_1649|Compound:st011737|FD-Score:-3.92|P-value:1.14E-4 ID:SGTC_1677|Compound:st019204|FD-Score:4.65|P-value:6.03E-6 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:5.62|P-value:6.45E-8 ID:SGTC_1713|Compound:st033146|FD-Score:5.54|P-value:9.34E-8 ID:SGTC_1714|Compound:st033236|FD-Score:7.47|P-value:1.10E-12 ID:SGTC_1719|Compound:st032227|FD-Score:5.91|P-value:1.37E-8 ID:SGTC_1786|Compound:berberine|FD-Score:-3.76|P-value:2.06E-4 ID:SGTC_1941|Compound:st076221|FD-Score:-4.60|P-value:7.45E-6 ID:SGTC_1945|Compound:st075991|FD-Score:3.62|P-value:3.27E-4 ID:SGTC_1992|Compound:st073643|FD-Score:4.35|P-value:2.12E-5 ID:SGTC_2066|Compound:5222451|FD-Score:-3.35|P-value:8.24E-4 ID:SGTC_2072|Compound:5231554|FD-Score:4.27|P-value:2.98E-5 ID:SGTC_2111|Compound:5486399|FD-Score:-3.52|P-value:4.59E-4 ID:SGTC_2124|Compound:5246320|FD-Score:-3.46|P-value:5.75E-4 ID:SGTC_2180|Compound:6301957|FD-Score:-3.60|P-value:3.51E-4 ID:SGTC_2246|Compound:7293507|FD-Score:-3.90|P-value:1.21E-4 ID:SGTC_2323|Compound:6148992|FD-Score:3.71|P-value:2.39E-4 ID:SGTC_2336|Compound:9001819|FD-Score:-4.00|P-value:8.43E-5 ID:SGTC_2342|Compound:7990637|FD-Score:4.69|P-value:5.17E-6 ID:SGTC_2344|Compound:7990960|FD-Score:-3.32|P-value:9.08E-4 ID:SGTC_2348|Compound:9022543|FD-Score:-3.32|P-value:9.06E-4 ID:SGTC_2350|Compound:9023895|FD-Score:-6.31|P-value:1.50E-9 ID:SGTC_2423|Compound:st077119|FD-Score:3.93|P-value:1.09E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-5.08|P-value:8.98E-7 ID:SGTC_2739|Compound:tacrine|FD-Score:-3.95|P-value:9.99E-5 ID:SGTC_2756|Compound:carbadox|FD-Score:-3.58|P-value:3.78E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:-3.34|P-value:8.30E-4 ID:SGTC_2781|Compound:5567276|FD-Score:-4.99|P-value:1.32E-6 ID:SGTC_2825|Compound:7998094|FD-Score:3.30|P-value:9.69E-4 ID:SGTC_2838|Compound:9002687|FD-Score:4.83|P-value:2.74E-6 ID:SGTC_2930|Compound:9013230|FD-Score:-3.51|P-value:4.82E-4 ID:SGTC_3071|Compound:9117417|FD-Score:3.89|P-value:1.27E-4 ID:SGTC_3072|Compound:9116419|FD-Score:-5.17|P-value:5.77E-7 ID:SGTC_3222|Compound:9128301|FD-Score:-4.71|P-value:4.73E-6 ID:SGTC_3249|Compound:9135183|FD-Score:-4.32|P-value:2.40E-5 ID:SGTC_3287|Compound:9117173|FD-Score:-4.35|P-value:2.15E-5 ID:SGTC_3294|Compound:9122953|FD-Score:5.34|P-value:2.56E-7 ID:SGTC_3334|Compound:9142812|FD-Score:3.31|P-value:9.21E-4

Top fitness defect scores for YNL061W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1714 st033236 7.47 13.6 µM 1.10E-12 1 4.12E-14 1.88
2 SGTC_610 4215-0184 6.99 155.0 µM 2.50E-11 exosome 8 1.40E-12 0.23
3 SGTC_1074 carmofur 5.97 3.8 µM 9.79E-9 exosome 15 1.17E-9 0.07
4 SGTC_1719 st032227 5.91 49.2 µM 1.37E-8 3 1.71E-9 0.74
5 SGTC_879 0831-1030 5.77 162.0 µM 2.94E-8 5 4.05E-9 0.37
6 SGTC_1698 5-fluorocytosine 5.62 1.1 µM 6.45E-8 exosome 11 9.82E-9 0.04
7 SGTC_1713 st033146 5.54 12.8 µM 9.34E-8 2 1.49E-8 0.37
8 SGTC_3294 9122953 5.34 26.3 µM 2.56E-7 tubulin folding & SWR complex 1 4.64E-8 0.89
9 SGTC_989 1501-0895 5.22 10.3 µM 4.48E-7 1 8.72E-8 1.12
10 SGTC_675 1309-0189 5.20 238.0 µM 5.07E-7 2 1.00E-7 1.04
11 SGTC_653 0203-0016 5.05 55.2 µM 1.04E-6 2 2.26E-7 0.78
12 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 4.93 4.6 µM 1.74E-6 heat shock/prefoldin 14 4.03E-7 0.05
13 SGTC_2838 9002687 4.83 71.4 µM 2.74E-6 fatty acid desaturase (OLE1) 9 6.69E-7 0.31
14 SGTC_2342 7990637 4.69 118.9 µM 5.17E-6 RNA processing & uracil transport 4 1.37E-6 0.45
15 SGTC_1677 st019204 4.65 53.9 µM 6.03E-6 3 1.63E-6 0.02
16 SGTC_640 3381-0960 4.62 37.1 µM 6.96E-6 3 1.91E-6 0.27
17 SGTC_641 4092-0595 4.50 30.0 µM 1.16E-5 5 3.41E-6 0.04
18 SGTC_261 5-fluorouridine 4.47 15.4 µM 1.31E-5 exosome 10 3.91E-6 0.14
19 SGTC_1992 st073643 4.35 23.3 µM 2.12E-5 mitochondrial processes 7 6.71E-6 0.13
20 SGTC_423 5-fluorouracil 4.31 457.0 µM 2.52E-5 exosome 24 8.12E-6 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.292 1.08E-66 YOL021C DIS3 het Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
0.277 3.11E-60 YGR195W SKI6 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.256 4.17E-51 YDL111C RRP42 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
0.240 4.37E-45 YGR095C RRP46 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
0.237 5.24E-44 YGR158C MTR3 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
0.217 7.28E-37 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.209 2.53E-34 YDR280W RRP45 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
0.203 1.38E-32 YPL043W NOP4 het Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
0.194 1.37E-29 YOR206W NOC2 het Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
0.193 1.79E-29 YLR009W RLP24 het Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
0.182 3.05E-26 YKL172W EBP2 het Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.179 1.52E-25 YPR142C_d YPR142C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
0.171 1.80E-23 YNL232W CSL4 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
0.162 3.98E-21 YPR143W RRP15 het Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
0.150 3.03E-18 YHR069C RRP4 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)