YNL062C / GCD10

Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL062C / GCD10

Click on Significant Values for Screen Details ID:SGTC_127|Compound:0180-0333|FD-Score:-5.62|P-value:7.59E-7 ID:SGTC_129|Compound:0088-0017|FD-Score:4.11|P-value:2.23E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:-3.67|P-value:8.38E-4 ID:SGTC_132|Compound:0417-1715|FD-Score:5.62|P-value:7.65E-7 ID:SGTC_195|Compound:4234-0266|FD-Score:6.74|P-value:4.22E-9 ID:SGTC_306|Compound:1672-3436|FD-Score:4.10|P-value:2.29E-4 ID:SGTC_347|Compound:4534-1311|FD-Score:3.84|P-value:5.11E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:3.75|P-value:6.79E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-5.26|P-value:3.33E-6 ID:SGTC_690|Compound:glyoxal|FD-Score:4.49|P-value:6.12E-5 ID:SGTC_736|Compound:0958-0254|FD-Score:3.70|P-value:7.72E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:-3.67|P-value:8.37E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:4.65|P-value:3.51E-5 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-4.07|P-value:2.48E-4 ID:SGTC_1452|Compound:4194-0072|FD-Score:3.88|P-value:4.60E-4 ID:SGTC_1675|Compound:st018485|FD-Score:-4.28|P-value:1.23E-4 ID:SGTC_1786|Compound:berberine|FD-Score:8.20|P-value:1.16E-12 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.91|P-value:4.09E-4 ID:SGTC_1977|Compound:st070720|FD-Score:4.28|P-value:1.28E-4 ID:SGTC_2010|Compound:4012422|FD-Score:-4.60|P-value:4.02E-5 ID:SGTC_2011|Compound:4012611|FD-Score:-3.64|P-value:9.20E-4 ID:SGTC_2071|Compound:5230951|FD-Score:-3.61|P-value:9.80E-4 ID:SGTC_2160|Compound:5660466|FD-Score:4.22|P-value:1.55E-4 ID:SGTC_2162|Compound:5663267|FD-Score:7.26|P-value:2.61E-10 ID:SGTC_2396|Compound:5342950|FD-Score:5.67|P-value:6.35E-7 ID:SGTC_2477|Compound:5967686|FD-Score:3.75|P-value:6.82E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:4.47|P-value:6.74E-5 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.67|P-value:8.59E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:8.46|P-value:2.25E-13 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.01|P-value:9.26E-6 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-3.91|P-value:4.04E-4 ID:SGTC_2795|Compound:7780010|FD-Score:-3.62|P-value:9.69E-4 ID:SGTC_2806|Compound:7952793|FD-Score:3.65|P-value:9.02E-4 ID:SGTC_2810|Compound:7959871|FD-Score:5.35|P-value:2.38E-6 ID:SGTC_2834|Compound:9001755|FD-Score:-3.73|P-value:6.97E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.45|P-value:6.95E-5 ID:SGTC_2966|Compound:9084041|FD-Score:3.94|P-value:3.85E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-4.36|P-value:9.49E-5 ID:SGTC_2975|Compound:9084847|FD-Score:4.30|P-value:1.19E-4 ID:SGTC_3085|Compound:9117376|FD-Score:3.71|P-value:7.69E-4 ID:SGTC_3199|Compound:9111318|FD-Score:-6.69|P-value:5.07E-9 ID:SGTC_3212|Compound:9128307|FD-Score:4.30|P-value:1.18E-4 ID:SGTC_3254|Compound:9136651|FD-Score:3.85|P-value:4.95E-4 ID:SGTC_3351|Compound:9150312|FD-Score:-4.47|P-value:6.36E-5 ID:SGTC_127|Compound:0180-0333|FD-Score:-5.62|P-value:7.59E-7 ID:SGTC_129|Compound:0088-0017|FD-Score:4.11|P-value:2.23E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:-3.67|P-value:8.38E-4 ID:SGTC_132|Compound:0417-1715|FD-Score:5.62|P-value:7.65E-7 ID:SGTC_195|Compound:4234-0266|FD-Score:6.74|P-value:4.22E-9 ID:SGTC_306|Compound:1672-3436|FD-Score:4.10|P-value:2.29E-4 ID:SGTC_347|Compound:4534-1311|FD-Score:3.84|P-value:5.11E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:3.75|P-value:6.79E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:-5.26|P-value:3.33E-6 ID:SGTC_690|Compound:glyoxal|FD-Score:4.49|P-value:6.12E-5 ID:SGTC_736|Compound:0958-0254|FD-Score:3.70|P-value:7.72E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:-3.67|P-value:8.37E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:4.65|P-value:3.51E-5 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-4.07|P-value:2.48E-4 ID:SGTC_1452|Compound:4194-0072|FD-Score:3.88|P-value:4.60E-4 ID:SGTC_1675|Compound:st018485|FD-Score:-4.28|P-value:1.23E-4 ID:SGTC_1786|Compound:berberine|FD-Score:8.20|P-value:1.16E-12 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.91|P-value:4.09E-4 ID:SGTC_1977|Compound:st070720|FD-Score:4.28|P-value:1.28E-4 ID:SGTC_2010|Compound:4012422|FD-Score:-4.60|P-value:4.02E-5 ID:SGTC_2011|Compound:4012611|FD-Score:-3.64|P-value:9.20E-4 ID:SGTC_2071|Compound:5230951|FD-Score:-3.61|P-value:9.80E-4 ID:SGTC_2160|Compound:5660466|FD-Score:4.22|P-value:1.55E-4 ID:SGTC_2162|Compound:5663267|FD-Score:7.26|P-value:2.61E-10 ID:SGTC_2396|Compound:5342950|FD-Score:5.67|P-value:6.35E-7 ID:SGTC_2477|Compound:5967686|FD-Score:3.75|P-value:6.82E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:4.47|P-value:6.74E-5 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.67|P-value:8.59E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:8.46|P-value:2.25E-13 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.01|P-value:9.26E-6 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-3.91|P-value:4.04E-4 ID:SGTC_2795|Compound:7780010|FD-Score:-3.62|P-value:9.69E-4 ID:SGTC_2806|Compound:7952793|FD-Score:3.65|P-value:9.02E-4 ID:SGTC_2810|Compound:7959871|FD-Score:5.35|P-value:2.38E-6 ID:SGTC_2834|Compound:9001755|FD-Score:-3.73|P-value:6.97E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.45|P-value:6.95E-5 ID:SGTC_2966|Compound:9084041|FD-Score:3.94|P-value:3.85E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-4.36|P-value:9.49E-5 ID:SGTC_2975|Compound:9084847|FD-Score:4.30|P-value:1.19E-4 ID:SGTC_3085|Compound:9117376|FD-Score:3.71|P-value:7.69E-4 ID:SGTC_3199|Compound:9111318|FD-Score:-6.69|P-value:5.07E-9 ID:SGTC_3212|Compound:9128307|FD-Score:4.30|P-value:1.18E-4 ID:SGTC_3254|Compound:9136651|FD-Score:3.85|P-value:4.95E-4 ID:SGTC_3351|Compound:9150312|FD-Score:-4.47|P-value:6.36E-5

Top fitness defect scores for YNL062C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2567 aureobasidin a 8.46 60.0 nM 2.25E-13 15 1.28E-17 0.25
2 SGTC_1786 berberine 8.20 21.6 µM 1.16E-12 60S ribosome export 5 1.19E-16 1.54
3 SGTC_2162 5663267 7.26 200.0 µM 2.61E-10 1 1.87E-13 1.62
4 SGTC_195 4234-0266 6.74 16.6 µM 4.22E-9 4 8.19E-12 0.69
5 SGTC_2396 5342950 5.67 42.8 µM 6.35E-7 RSC complex & mRNA processing 14 7.31E-9 0.31
6 SGTC_132 0417-1715 5.62 25.8 µM 7.65E-7 RPP1 & pyrimidine depletion 5 9.42E-9 0.60
7 SGTC_2810 7959871 5.35 45.5 µM 2.38E-6 copper-dependent oxidative stress 9 4.37E-8 0.03
8 SGTC_2679 rapamycin 5.01 1.0 nM 9.26E-6 36 2.74E-7 0.02
9 SGTC_1105 epirubicin 4.65 18.1 µM 3.51E-5 anthracycline transcription coupled DNA repair 6 1.66E-6 0.20
10 SGTC_690 glyoxal 4.49 20.3 µM 6.12E-5 21 3.50E-6 0.06
11 SGTC_2513 baicalein 4.47 25.6 µM 6.74E-5 calcium & mitochondrial duress 3 3.99E-6 0.39
12 SGTC_3212 9128307 4.30 49.5 µM 1.18E-4 7 8.46E-6 0.12
13 SGTC_2975 9084847 4.30 53.7 µM 1.19E-4 5 8.62E-6 0.00
14 SGTC_1977 st070720 4.28 65.3 µM 1.28E-4 2 9.48E-6 0.82
15 SGTC_2160 5660466 4.22 132.9 µM 1.55E-4 60S ribosome export 14 1.22E-5 0.26
16 SGTC_129 0088-0017 4.11 176.6 µM 2.23E-4 2 2.00E-5 0.25
17 SGTC_306 1672-3436 4.10 11.6 µM 2.29E-4 Golgi 3 2.07E-5 0.43
18 SGTC_2966 9084041 3.94 48.0 µM 3.85E-4 2 4.16E-5 0.07
19 SGTC_1452 4194-0072 3.88 190.0 µM 4.60E-4 5 5.28E-5 0.23
20 SGTC_3254 9136651 3.85 49.5 µM 4.95E-4 DNA damage response 7 5.82E-5 0.25

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.205 4.90E-33 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.114 3.81E-11 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.111 1.04E-10 YGR008C STF2 hom Protein involved in resistance to dessication stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to the F0 sector of mitochondrial F1F0 ATPase in vitro and is proposed to modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress
0.109 2.25E-10 YJL211C_d YJL211C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2
0.108 3.25E-10 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.106 6.42E-10 YBR110W ALG1 het Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog
0.104 1.84E-9 YPR182W SMX3 het Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F
0.101 4.19E-9 YJR013W GPI14 het Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.095 4.23E-8 YPR025C CCL1 het Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters
0.092 8.40E-8 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.091 1.41E-7 YMR211W DML1 het Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
0.088 3.72E-7 YPL264C_p YPL264C_p hom Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene
0.087 5.06E-7 YHL003C LAG1 hom Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress
0.086 6.30E-7 YIL025C_d YIL025C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.084 9.80E-7 YBL086C_p YBL086C_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery