YNL073W / MSK1

Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys)

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL073W / MSK1

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:4.57|P-value:8.46E-6 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:3.34|P-value:8.17E-4 ID:SGTC_63|Compound:0099-0242|FD-Score:5.53|P-value:9.95E-8 ID:SGTC_68|Compound:0986-0284|FD-Score:3.83|P-value:1.57E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:4.50|P-value:1.16E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:3.75|P-value:2.04E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:5.11|P-value:7.73E-7 ID:SGTC_236|Compound:1509-0011|FD-Score:8.31|P-value:2.94E-15 ID:SGTC_275|Compound:trichlorophene|FD-Score:3.59|P-value:3.55E-4 ID:SGTC_283|Compound:0987-0079|FD-Score:3.82|P-value:1.58E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.74|P-value:4.80E-6 ID:SGTC_309|Compound:k408-0051|FD-Score:-4.58|P-value:9.55E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:12.00|P-value:9.53E-30 ID:SGTC_339|Compound:3091-4833|FD-Score:-3.80|P-value:1.93E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:-4.12|P-value:5.95E-5 ID:SGTC_374|Compound:0880-0231|FD-Score:-4.11|P-value:6.23E-5 ID:SGTC_384|Compound:k007-0144|FD-Score:-4.40|P-value:1.96E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-6.51|P-value:5.90E-10 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.30|P-value:2.95E-5 ID:SGTC_735|Compound:3076-0345|FD-Score:3.87|P-value:1.33E-4 ID:SGTC_767|Compound:0929-0023|FD-Score:3.38|P-value:7.20E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:4.31|P-value:2.49E-5 ID:SGTC_908|Compound:3448-7491|FD-Score:4.19|P-value:3.94E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:4.89|P-value:2.07E-6 ID:SGTC_965|Compound:1291-5240|FD-Score:-3.75|P-value:2.37E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:4.68|P-value:5.35E-6 ID:SGTC_1074|Compound:carmofur|FD-Score:5.51|P-value:1.11E-7 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:3.93|P-value:1.07E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.53|P-value:4.35E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:4.22|P-value:3.59E-5 ID:SGTC_1244|Compound:0416-0015|FD-Score:-3.48|P-value:5.87E-4 ID:SGTC_1627|Compound:st003713|FD-Score:3.51|P-value:4.68E-4 ID:SGTC_1695|Compound:st026914|FD-Score:3.31|P-value:9.10E-4 ID:SGTC_1742|Compound:st038235|FD-Score:5.53|P-value:1.01E-7 ID:SGTC_1960|Compound:st011366|FD-Score:3.89|P-value:1.23E-4 ID:SGTC_2008|Compound:4012253|FD-Score:3.90|P-value:1.22E-4 ID:SGTC_2166|Compound:5933061|FD-Score:-3.42|P-value:7.24E-4 ID:SGTC_2278|Compound:7948257|FD-Score:4.12|P-value:5.23E-5 ID:SGTC_2295|Compound:7971645|FD-Score:3.54|P-value:4.32E-4 ID:SGTC_2460|Compound:5552655|FD-Score:3.57|P-value:3.91E-4 ID:SGTC_2518|Compound:prenyletin|FD-Score:3.43|P-value:6.11E-4 ID:SGTC_2523|Compound:spermine|FD-Score:3.37|P-value:7.59E-4 ID:SGTC_2526|Compound:patulin|FD-Score:6.08|P-value:5.45E-9 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.60|P-value:3.51E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:3.49|P-value:5.04E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:3.95|P-value:9.91E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:5.14|P-value:6.66E-7 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-3.38|P-value:8.25E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:-3.82|P-value:1.81E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.92|P-value:1.14E-4 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:3.60|P-value:3.43E-4 ID:SGTC_2646|Compound:retinol|FD-Score:3.88|P-value:1.29E-4 ID:SGTC_2682|Compound:pyrogallin|FD-Score:4.39|P-value:1.80E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.09|P-value:5.19E-9 ID:SGTC_2811|Compound:7967822|FD-Score:-5.39|P-value:2.37E-7 ID:SGTC_2812|Compound:7970281|FD-Score:3.44|P-value:5.99E-4 ID:SGTC_2815|Compound:7968870|FD-Score:-3.85|P-value:1.62E-4 ID:SGTC_2940|Compound:9060638|FD-Score:3.35|P-value:7.96E-4 ID:SGTC_3104|Compound:9120930|FD-Score:-3.88|P-value:1.49E-4 ID:SGTC_10|Compound:0279-0212|FD-Score:4.57|P-value:8.46E-6 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:3.34|P-value:8.17E-4 ID:SGTC_63|Compound:0099-0242|FD-Score:5.53|P-value:9.95E-8 ID:SGTC_68|Compound:0986-0284|FD-Score:3.83|P-value:1.57E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:4.50|P-value:1.16E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:3.75|P-value:2.04E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:5.11|P-value:7.73E-7 ID:SGTC_236|Compound:1509-0011|FD-Score:8.31|P-value:2.94E-15 ID:SGTC_275|Compound:trichlorophene|FD-Score:3.59|P-value:3.55E-4 ID:SGTC_283|Compound:0987-0079|FD-Score:3.82|P-value:1.58E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.74|P-value:4.80E-6 ID:SGTC_309|Compound:k408-0051|FD-Score:-4.58|P-value:9.55E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:12.00|P-value:9.53E-30 ID:SGTC_339|Compound:3091-4833|FD-Score:-3.80|P-value:1.93E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:-4.12|P-value:5.95E-5 ID:SGTC_374|Compound:0880-0231|FD-Score:-4.11|P-value:6.23E-5 ID:SGTC_384|Compound:k007-0144|FD-Score:-4.40|P-value:1.96E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-6.51|P-value:5.90E-10 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.30|P-value:2.95E-5 ID:SGTC_735|Compound:3076-0345|FD-Score:3.87|P-value:1.33E-4 ID:SGTC_767|Compound:0929-0023|FD-Score:3.38|P-value:7.20E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:4.31|P-value:2.49E-5 ID:SGTC_908|Compound:3448-7491|FD-Score:4.19|P-value:3.94E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:4.89|P-value:2.07E-6 ID:SGTC_965|Compound:1291-5240|FD-Score:-3.75|P-value:2.37E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:4.68|P-value:5.35E-6 ID:SGTC_1074|Compound:carmofur|FD-Score:5.51|P-value:1.11E-7 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:3.93|P-value:1.07E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.53|P-value:4.35E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:4.22|P-value:3.59E-5 ID:SGTC_1244|Compound:0416-0015|FD-Score:-3.48|P-value:5.87E-4 ID:SGTC_1627|Compound:st003713|FD-Score:3.51|P-value:4.68E-4 ID:SGTC_1695|Compound:st026914|FD-Score:3.31|P-value:9.10E-4 ID:SGTC_1742|Compound:st038235|FD-Score:5.53|P-value:1.01E-7 ID:SGTC_1960|Compound:st011366|FD-Score:3.89|P-value:1.23E-4 ID:SGTC_2008|Compound:4012253|FD-Score:3.90|P-value:1.22E-4 ID:SGTC_2166|Compound:5933061|FD-Score:-3.42|P-value:7.24E-4 ID:SGTC_2278|Compound:7948257|FD-Score:4.12|P-value:5.23E-5 ID:SGTC_2295|Compound:7971645|FD-Score:3.54|P-value:4.32E-4 ID:SGTC_2460|Compound:5552655|FD-Score:3.57|P-value:3.91E-4 ID:SGTC_2518|Compound:prenyletin|FD-Score:3.43|P-value:6.11E-4 ID:SGTC_2523|Compound:spermine|FD-Score:3.37|P-value:7.59E-4 ID:SGTC_2526|Compound:patulin|FD-Score:6.08|P-value:5.45E-9 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.60|P-value:3.51E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:3.49|P-value:5.04E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:3.95|P-value:9.91E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:5.14|P-value:6.66E-7 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-3.38|P-value:8.25E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:-3.82|P-value:1.81E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.92|P-value:1.14E-4 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:3.60|P-value:3.43E-4 ID:SGTC_2646|Compound:retinol|FD-Score:3.88|P-value:1.29E-4 ID:SGTC_2682|Compound:pyrogallin|FD-Score:4.39|P-value:1.80E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.09|P-value:5.19E-9 ID:SGTC_2811|Compound:7967822|FD-Score:-5.39|P-value:2.37E-7 ID:SGTC_2812|Compound:7970281|FD-Score:3.44|P-value:5.99E-4 ID:SGTC_2815|Compound:7968870|FD-Score:-3.85|P-value:1.62E-4 ID:SGTC_2940|Compound:9060638|FD-Score:3.35|P-value:7.96E-4 ID:SGTC_3104|Compound:9120930|FD-Score:-3.88|P-value:1.49E-4

Top fitness defect scores for YNL073W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_310 1273-0058 12.00 1.1 µM 9.53E-30 mitochondrial stress 22 1.73E-33
2 SGTC_236 1509-0011 8.31 5.3 µM 2.94E-15 240 4.79E-17
3 SGTC_2688 kanamycin b 6.09 96.7 µM 5.19E-9 calcium & mitochondrial duress 154 5.69E-10
4 SGTC_2526 patulin 6.08 37.6 µM 5.45E-9 25 6.00E-10
5 SGTC_63 0099-0242 5.53 26.7 µM 9.95E-8 mitochondrial stress 36 1.60E-8
6 SGTC_1742 st038235 5.53 28.9 µM 1.01E-7 mitochondrial stress 60 1.63E-8
7 SGTC_1074 carmofur 5.51 3.8 µM 1.11E-7 exosome 6 1.81E-8
8 SGTC_2574 2',4'-dihydroxychalcone 5.14 14.6 µM 6.66E-7 10 1.37E-7
9 SGTC_173 1273-0061 5.11 2.7 µM 7.73E-7 mitochondrial stress 142 1.62E-7
10 SGTC_932 2857-0803 4.89 154.0 µM 2.07E-6 16 4.95E-7
11 SGTC_1063 disulfiram 4.68 1.8 µM 5.35E-6 10 1.44E-6
12 SGTC_10 0279-0212 4.57 42.6 µM 8.46E-6 TRP & mitochondrial translation 88 2.42E-6
13 SGTC_70 0141-0289 4.50 43.3 µM 1.16E-5 mitochondrial stress 138 3.45E-6
14 SGTC_2682 pyrogallin 4.39 40.0 µM 1.80E-5 copper-dependent oxidative stress 18 5.67E-6
15 SGTC_832 4083-0019 4.31 164.0 µM 2.49E-5 translation 69 8.18E-6
16 SGTC_1240 0356-0016 4.22 42.1 µM 3.59E-5 mitochondrial response to ROS 31 1.23E-5
17 SGTC_908 3448-7491 4.19 47.7 µM 3.94E-5 mitochondrial stress 109 1.37E-5
18 SGTC_2278 7948257 4.12 134.3 µM 5.23E-5 20 1.89E-5
19 SGTC_2573 2',4'-dihydroxychalcone 3.95 15.0 µM 9.91E-5 49 3.87E-5
20 SGTC_1106 tegaserod maleate 3.93 11.7 µM 1.07E-4 16 4.22E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.420 7.88E-144 YLL033W IRC19 hom Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci
0.408 1.17E-134 YCR046C IMG1 hom Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome
0.404 4.74E-132 YNL005C MRP7 hom Mitochondrial ribosomal protein of the large subunit
0.398 6.60E-128 YMR228W MTF1 hom Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.397 2.36E-127 YER087W AIM10 hom Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss
0.396 3.64E-126 YGR215W RSM27 hom Mitochondrial ribosomal protein of the small subunit
0.396 4.18E-126 YMR193W MRPL24 hom Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
0.394 8.07E-125 YCR071C IMG2 hom Mitochondrial ribosomal protein of the large subunit
0.394 2.19E-124 YMR287C DSS1 hom 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.392 3.87E-123 YLR260W LCB5 hom Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules
0.389 1.42E-121 YLR439W MRPL4 hom Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit
0.385 5.23E-119 YDR347W MRP1 hom Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein
0.384 2.48E-118 YNL177C MRPL22 hom Mitochondrial ribosomal protein of the large subunit
0.381 3.30E-116 YLR069C MEF1 hom Mitochondrial elongation factor involved in translational elongation
0.379 8.63E-115 YNL081C SWS2 hom Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency