YNL076W / MKS1

Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL076W / MKS1

Click on Significant Values for Screen Details ID:SGTC_48|Compound:0382-0018|FD-Score:3.04|P-value:6.01E-4 ID:SGTC_51|Compound:1013-0126|FD-Score:3.08|P-value:5.28E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.75|P-value:2.02E-7 ID:SGTC_151|Compound:3013-0144|FD-Score:2.95|P-value:8.57E-4 ID:SGTC_254|Compound:nsc-64875|FD-Score:-3.75|P-value:2.69E-5 ID:SGTC_258|Compound:haloperidol|FD-Score:-3.91|P-value:1.30E-5 ID:SGTC_269|Compound:exalamide|FD-Score:4.32|P-value:2.00E-6 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:3.23|P-value:2.93E-4 ID:SGTC_500|Compound:h-89|FD-Score:3.61|P-value:6.09E-5 ID:SGTC_668|Compound:1682-0499|FD-Score:-3.56|P-value:6.33E-5 ID:SGTC_670|Compound:4204-0025|FD-Score:2.97|P-value:7.88E-4 ID:SGTC_680|Compound:0417-1641|FD-Score:-3.13|P-value:3.74E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:3.18|P-value:3.49E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:-4.02|P-value:7.55E-6 ID:SGTC_976|Compound:1348-1067|FD-Score:3.19|P-value:3.34E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:-2.92|P-value:8.30E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.98|P-value:9.33E-6 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.15|P-value:3.90E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.10|P-value:4.84E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:3.09|P-value:5.00E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:-2.90|P-value:8.99E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:3.15|P-value:3.96E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:5.70|P-value:6.00E-10 ID:SGTC_1596|Compound:piperlongumine|FD-Score:-3.00|P-value:6.17E-4 ID:SGTC_1757|Compound:st041620|FD-Score:-3.63|P-value:4.61E-5 ID:SGTC_1777|Compound:st045864|FD-Score:-3.63|P-value:4.76E-5 ID:SGTC_1965|Compound:Pectolinarigenin|FD-Score:2.99|P-value:7.22E-4 ID:SGTC_1976|Compound:st070609|FD-Score:4.25|P-value:2.93E-6 ID:SGTC_2038|Compound:4100038|FD-Score:3.89|P-value:1.67E-5 ID:SGTC_2266|Compound:7929956|FD-Score:-2.97|P-value:6.80E-4 ID:SGTC_2318|Compound:6029776|FD-Score:3.73|P-value:3.49E-5 ID:SGTC_2400|Compound:5203494|FD-Score:3.29|P-value:2.27E-4 ID:SGTC_2488|Compound:5268135|FD-Score:3.73|P-value:3.55E-5 ID:SGTC_2687|Compound:chenodiol|FD-Score:4.15|P-value:4.91E-6 ID:SGTC_2755|Compound:lomerizine|FD-Score:-5.85|P-value:1.60E-10 ID:SGTC_2757|Compound:clomiphene|FD-Score:5.82|P-value:2.50E-10 ID:SGTC_3057|Compound:9095946|FD-Score:3.24|P-value:2.78E-4 ID:SGTC_3137|Compound:9097352|FD-Score:2.97|P-value:7.89E-4 ID:SGTC_3177|Compound:9107506|FD-Score:5.07|P-value:3.23E-8 ID:SGTC_3186|Compound:9110464|FD-Score:3.39|P-value:1.49E-4 ID:SGTC_3343|Compound:9148379|FD-Score:3.68|P-value:4.29E-5 ID:SGTC_48|Compound:0382-0018|FD-Score:3.04|P-value:6.01E-4 ID:SGTC_51|Compound:1013-0126|FD-Score:3.08|P-value:5.28E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.75|P-value:2.02E-7 ID:SGTC_151|Compound:3013-0144|FD-Score:2.95|P-value:8.57E-4 ID:SGTC_254|Compound:nsc-64875|FD-Score:-3.75|P-value:2.69E-5 ID:SGTC_258|Compound:haloperidol|FD-Score:-3.91|P-value:1.30E-5 ID:SGTC_269|Compound:exalamide|FD-Score:4.32|P-value:2.00E-6 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:3.23|P-value:2.93E-4 ID:SGTC_500|Compound:h-89|FD-Score:3.61|P-value:6.09E-5 ID:SGTC_668|Compound:1682-0499|FD-Score:-3.56|P-value:6.33E-5 ID:SGTC_670|Compound:4204-0025|FD-Score:2.97|P-value:7.88E-4 ID:SGTC_680|Compound:0417-1641|FD-Score:-3.13|P-value:3.74E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:3.18|P-value:3.49E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:-4.02|P-value:7.55E-6 ID:SGTC_976|Compound:1348-1067|FD-Score:3.19|P-value:3.34E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:-2.92|P-value:8.30E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.98|P-value:9.33E-6 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.15|P-value:3.90E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.10|P-value:4.84E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:3.09|P-value:5.00E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:-2.90|P-value:8.99E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:3.15|P-value:3.96E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:5.70|P-value:6.00E-10 ID:SGTC_1596|Compound:piperlongumine|FD-Score:-3.00|P-value:6.17E-4 ID:SGTC_1757|Compound:st041620|FD-Score:-3.63|P-value:4.61E-5 ID:SGTC_1777|Compound:st045864|FD-Score:-3.63|P-value:4.76E-5 ID:SGTC_1965|Compound:Pectolinarigenin|FD-Score:2.99|P-value:7.22E-4 ID:SGTC_1976|Compound:st070609|FD-Score:4.25|P-value:2.93E-6 ID:SGTC_2038|Compound:4100038|FD-Score:3.89|P-value:1.67E-5 ID:SGTC_2266|Compound:7929956|FD-Score:-2.97|P-value:6.80E-4 ID:SGTC_2318|Compound:6029776|FD-Score:3.73|P-value:3.49E-5 ID:SGTC_2400|Compound:5203494|FD-Score:3.29|P-value:2.27E-4 ID:SGTC_2488|Compound:5268135|FD-Score:3.73|P-value:3.55E-5 ID:SGTC_2687|Compound:chenodiol|FD-Score:4.15|P-value:4.91E-6 ID:SGTC_2755|Compound:lomerizine|FD-Score:-5.85|P-value:1.60E-10 ID:SGTC_2757|Compound:clomiphene|FD-Score:5.82|P-value:2.50E-10 ID:SGTC_3057|Compound:9095946|FD-Score:3.24|P-value:2.78E-4 ID:SGTC_3137|Compound:9097352|FD-Score:2.97|P-value:7.89E-4 ID:SGTC_3177|Compound:9107506|FD-Score:5.07|P-value:3.23E-8 ID:SGTC_3186|Compound:9110464|FD-Score:3.39|P-value:1.49E-4 ID:SGTC_3343|Compound:9148379|FD-Score:3.68|P-value:4.29E-5

Top fitness defect scores for YNL076W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2757 clomiphene 5.82 4.2 µM 2.50E-10 fatty acid desaturase (OLE1) 10 2.86E-9
2 SGTC_1595 6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin 5.70 67.5 µM 6.00E-10 11 6.17E-9
3 SGTC_3177 9107506 5.07 49.5 µM 3.23E-8 ERG2 4 2.04E-7
4 SGTC_59 0330-0160 4.75 1.3 µM 2.02E-7 74 1.02E-6
5 SGTC_269 exalamide 4.32 29.0 µM 2.00E-6 ergosterol biosynthesis 15 7.69E-6
6 SGTC_1976 st070609 4.25 63.5 µM 2.93E-6 7 1.07E-5
7 SGTC_2687 chenodiol 4.15 100.0 µM 4.91E-6 18 1.69E-5
8 SGTC_2038 4100038 3.89 31.0 µM 1.67E-5 plasma membrane duress 54 4.98E-5
9 SGTC_2318 6029776 3.73 164.0 µM 3.49E-5 36 9.54E-5
10 SGTC_2488 5268135 3.73 45.7 µM 3.55E-5 58 9.67E-5
11 SGTC_3343 9148379 3.68 19.0 µM 4.29E-5 7 1.14E-4
12 SGTC_500 h-89 3.61 112.0 µM 6.09E-5 cell wall signaling 37 1.56E-4
13 SGTC_3186 9110464 3.39 49.5 µM 1.49E-4 RPP1 & pyrimidine depletion 27 3.44E-4
14 SGTC_2400 5203494 3.29 178.7 µM 2.27E-4 RSC complex & mRNA processing 42 4.98E-4
15 SGTC_3057 9095946 3.24 49.5 µM 2.78E-4 Golgi 30 5.95E-4
16 SGTC_390 dec-rvkr-cmk 3.23 16.7 µM 2.93E-4 calcium & mitochondrial duress 62 6.25E-4
17 SGTC_976 1348-1067 3.19 47.6 µM 3.34E-4 60S ribosome export 63 7.01E-4
18 SGTC_759 3486-0358 3.18 89.3 µM 3.49E-4 41 7.28E-4
19 SGTC_1424 4048-1941 3.15 275.0 µM 3.90E-4 61 8.04E-4
20 SGTC_1581 himbacine 3.15 57.9 µM 3.96E-4 Golgi 75 8.13E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.198 7.08E-31 YLR211C_p YLR211C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron
0.144 6.38E-17 YGL230C_p YGL230C_p hom Putative protein of unknown function; non-essential gene
0.126 2.07E-13 YNL260C LTO1 het Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
0.092 8.86E-8 YDL184C RPL41A hom Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication
0.088 3.61E-7 YGL082W_p YGL082W_p hom Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene
0.084 1.25E-6 YER093C TSC11 het Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain
0.083 1.71E-6 YGR177C ATF2 hom Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking
0.081 2.34E-6 YNL312W RFA2 het Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
0.076 1.14E-5 YNL088W TOP2 het Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation
0.075 1.33E-5 YLR064W PER33 hom Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress
0.075 1.37E-5 YBR225W_p YBR225W_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components
0.073 2.58E-5 YLL012W YEH1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.072 2.75E-5 YLR035C MLH2 hom Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p
0.072 3.13E-5 YOR087W YVC1 hom Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock
0.072 3.22E-5 YNL078W NIS1 hom Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network