YNL077W / APJ1

Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL077W / APJ1

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:-3.62|P-value:8.00E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:-3.55|P-value:9.75E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-4.41|P-value:6.03E-5 ID:SGTC_81|Compound:0411-0046|FD-Score:4.00|P-value:2.51E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:4.17|P-value:1.45E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:3.60|P-value:8.91E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:10.60|P-value:1.27E-20 ID:SGTC_419|Compound:nsc-638432|FD-Score:3.66|P-value:7.40E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.25|P-value:2.34E-6 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:6.94|P-value:8.04E-10 ID:SGTC_568|Compound:1193-0097|FD-Score:7.43|P-value:5.25E-11 ID:SGTC_649|Compound:1123-0311|FD-Score:3.76|P-value:5.37E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:4.30|P-value:9.31E-5 ID:SGTC_777|Compound:1315-0167|FD-Score:5.93|P-value:1.29E-7 ID:SGTC_787|Compound:4152-0075|FD-Score:4.24|P-value:1.13E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:4.48|P-value:4.94E-5 ID:SGTC_874|Compound:0599-0792|FD-Score:-4.73|P-value:1.83E-5 ID:SGTC_1030|Compound:k029-0042|FD-Score:-3.81|P-value:4.44E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:3.83|P-value:4.38E-4 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.58|P-value:9.45E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:3.93|P-value:3.23E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:3.57|P-value:9.70E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:3.73|P-value:5.87E-4 ID:SGTC_1675|Compound:st018485|FD-Score:3.68|P-value:6.85E-4 ID:SGTC_1677|Compound:st019204|FD-Score:3.72|P-value:6.10E-4 ID:SGTC_1729|Compound:st035615|FD-Score:4.18|P-value:1.42E-4 ID:SGTC_1754|Compound:st042065|FD-Score:5.59|P-value:5.84E-7 ID:SGTC_1853|Compound:st056710|FD-Score:3.57|P-value:9.57E-4 ID:SGTC_1930|Compound:st074634|FD-Score:-4.45|P-value:5.23E-5 ID:SGTC_1935|Compound:st074532|FD-Score:-3.89|P-value:3.43E-4 ID:SGTC_1943|Compound:st076331|FD-Score:3.82|P-value:4.56E-4 ID:SGTC_2095|Compound:5432005|FD-Score:3.58|P-value:9.38E-4 ID:SGTC_2167|Compound:5876490|FD-Score:-4.27|P-value:9.71E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.43|P-value:6.00E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.77|P-value:5.33E-4 ID:SGTC_2305|Compound:7443522|FD-Score:4.59|P-value:3.26E-5 ID:SGTC_2307|Compound:7538926|FD-Score:3.64|P-value:7.91E-4 ID:SGTC_2398|Compound:5228213|FD-Score:6.03|P-value:8.05E-8 ID:SGTC_2417|Compound:cerulenin|FD-Score:-5.28|P-value:2.12E-6 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:4.88|P-value:1.09E-5 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:6.32|P-value:2.00E-8 ID:SGTC_2582|Compound:agaric acid|FD-Score:-6.19|P-value:3.49E-8 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.90|P-value:1.02E-5 ID:SGTC_2785|Compound:7756463|FD-Score:-3.87|P-value:3.70E-4 ID:SGTC_2849|Compound:9019321|FD-Score:3.85|P-value:4.10E-4 ID:SGTC_2867|Compound:9039098|FD-Score:5.57|P-value:6.39E-7 ID:SGTC_2922|Compound:7999257|FD-Score:5.77|P-value:2.67E-7 ID:SGTC_2971|Compound:9092186|FD-Score:4.69|P-value:2.30E-5 ID:SGTC_3037|Compound:9091026|FD-Score:4.79|P-value:1.54E-5 ID:SGTC_57|Compound:miconazole|FD-Score:-3.62|P-value:8.00E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:-3.55|P-value:9.75E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-4.41|P-value:6.03E-5 ID:SGTC_81|Compound:0411-0046|FD-Score:4.00|P-value:2.51E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:4.17|P-value:1.45E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:3.60|P-value:8.91E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:10.60|P-value:1.27E-20 ID:SGTC_419|Compound:nsc-638432|FD-Score:3.66|P-value:7.40E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.25|P-value:2.34E-6 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:6.94|P-value:8.04E-10 ID:SGTC_568|Compound:1193-0097|FD-Score:7.43|P-value:5.25E-11 ID:SGTC_649|Compound:1123-0311|FD-Score:3.76|P-value:5.37E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:4.30|P-value:9.31E-5 ID:SGTC_777|Compound:1315-0167|FD-Score:5.93|P-value:1.29E-7 ID:SGTC_787|Compound:4152-0075|FD-Score:4.24|P-value:1.13E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:4.48|P-value:4.94E-5 ID:SGTC_874|Compound:0599-0792|FD-Score:-4.73|P-value:1.83E-5 ID:SGTC_1030|Compound:k029-0042|FD-Score:-3.81|P-value:4.44E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:3.83|P-value:4.38E-4 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.58|P-value:9.45E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:3.93|P-value:3.23E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:3.57|P-value:9.70E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:3.73|P-value:5.87E-4 ID:SGTC_1675|Compound:st018485|FD-Score:3.68|P-value:6.85E-4 ID:SGTC_1677|Compound:st019204|FD-Score:3.72|P-value:6.10E-4 ID:SGTC_1729|Compound:st035615|FD-Score:4.18|P-value:1.42E-4 ID:SGTC_1754|Compound:st042065|FD-Score:5.59|P-value:5.84E-7 ID:SGTC_1853|Compound:st056710|FD-Score:3.57|P-value:9.57E-4 ID:SGTC_1930|Compound:st074634|FD-Score:-4.45|P-value:5.23E-5 ID:SGTC_1935|Compound:st074532|FD-Score:-3.89|P-value:3.43E-4 ID:SGTC_1943|Compound:st076331|FD-Score:3.82|P-value:4.56E-4 ID:SGTC_2095|Compound:5432005|FD-Score:3.58|P-value:9.38E-4 ID:SGTC_2167|Compound:5876490|FD-Score:-4.27|P-value:9.71E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.43|P-value:6.00E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.77|P-value:5.33E-4 ID:SGTC_2305|Compound:7443522|FD-Score:4.59|P-value:3.26E-5 ID:SGTC_2307|Compound:7538926|FD-Score:3.64|P-value:7.91E-4 ID:SGTC_2398|Compound:5228213|FD-Score:6.03|P-value:8.05E-8 ID:SGTC_2417|Compound:cerulenin|FD-Score:-5.28|P-value:2.12E-6 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:4.88|P-value:1.09E-5 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:6.32|P-value:2.00E-8 ID:SGTC_2582|Compound:agaric acid|FD-Score:-6.19|P-value:3.49E-8 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.90|P-value:1.02E-5 ID:SGTC_2785|Compound:7756463|FD-Score:-3.87|P-value:3.70E-4 ID:SGTC_2849|Compound:9019321|FD-Score:3.85|P-value:4.10E-4 ID:SGTC_2867|Compound:9039098|FD-Score:5.57|P-value:6.39E-7 ID:SGTC_2922|Compound:7999257|FD-Score:5.77|P-value:2.67E-7 ID:SGTC_2971|Compound:9092186|FD-Score:4.69|P-value:2.30E-5 ID:SGTC_3037|Compound:9091026|FD-Score:4.79|P-value:1.54E-5

Top fitness defect scores for YNL077W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_221 trifluoperazine 10.60 10.3 µM 1.27E-20 NEO1-PIK1 52 1.22E-26
2 SGTC_568 1193-0097 7.43 7.5 µM 5.25E-11 ERAD & cell cycle 42 5.52E-14
3 SGTC_457 grayanotoxin iii 6.94 135.0 µM 8.04E-10 15 1.99E-12
4 SGTC_2496 diffractaic acid 6.32 79.8 µM 2.00E-8 3 1.35E-10
5 SGTC_2398 5228213 6.03 48.8 µM 8.05E-8 iron homeostasis 8 8.39E-10
6 SGTC_777 1315-0167 5.93 517.0 µM 1.29E-7 Golgi 6 1.56E-9
7 SGTC_2922 7999257 5.77 32.5 µM 2.67E-7 3 4.04E-9
8 SGTC_1754 st042065 5.59 16.1 µM 5.84E-7 NEO1-PIK1 106 1.13E-8
9 SGTC_2867 9039098 5.57 71.4 µM 6.39E-7 iron homeostasis 8 1.27E-8
10 SGTC_2688 kanamycin b 4.90 96.7 µM 1.02E-5 calcium & mitochondrial duress 252 4.75E-7
11 SGTC_2420 aureobasidin a 4.88 50.0 nM 1.09E-5 20 5.18E-7
12 SGTC_3037 9091026 4.79 49.5 µM 1.54E-5 NEO1-PIK1 83 8.19E-7
13 SGTC_2971 9092186 4.69 9.9 µM 2.30E-5 ergosterol biosynthesis 16 1.38E-6
14 SGTC_2305 7443522 4.59 69.2 µM 3.26E-5 7 2.17E-6
15 SGTC_827 3253-0474 4.48 693.0 µM 4.94E-5 10 3.74E-6
16 SGTC_2249 haloperidol 4.43 116.4 µM 6.00E-5 ergosterol depletion effects on membrane 85 4.81E-6
17 SGTC_768 0384-0033 4.30 77.3 µM 9.31E-5 fatty acid desaturase (OLE1) 74 8.54E-6
18 SGTC_787 4152-0075 4.24 138.0 µM 1.13E-4 18 1.10E-5
19 SGTC_1729 st035615 4.18 46.0 µM 1.42E-4 32 1.49E-5
20 SGTC_127 0180-0333 4.17 59.1 µM 1.45E-4 mitochondrial response to ROS 35 1.52E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.231 5.50E-42 YGL077C HNM1 hom Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol
0.224 2.63E-39 YBR291C CTP1 hom Mitochondrial inner membrane citrate transporter, member of the mitochondrial carrier family
0.223 3.99E-39 YDL025C RTK1 hom Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress
0.221 2.03E-38 YBR245C ISW1 hom ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions
0.220 5.38E-38 YDR317W HIM1 hom Protein of unknown function involved in DNA repair
0.206 1.36E-33 YGR202C PCT1 hom Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
0.206 1.89E-33 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.203 1.44E-32 YOL144W NOP8 het Nucleolar protein required for 60S ribosomal subunit biogenesis
0.201 5.20E-32 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.197 1.45E-30 YGR060W ERG25 het C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
0.196 2.57E-30 YCR079W PTC6 hom Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase
0.195 3.22E-30 YNR044W AGA1 hom Anchorage subunit of a-agglutinin of a-cells, highly O-glycosylated protein with N-terminal secretion signal and C-terminal signal for addition of GPI anchor to cell wall, linked to adhesion subunit Aga2p via two disulfide bonds
0.189 2.88E-28 YOR115C TRS33 hom One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.185 2.92E-27 YGR166W TRS65 hom Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.184 9.22E-27 YNL295W_p YNL295W_p hom Putative protein of unknown function