HIPHOP chemogenomics database

YNL084C / END3

EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p

Zygosity: Homozygous strain

fixedexpanded
Profile for YNL084C / END3

Click on Significant Values for Screen Details

Top fitness defect scores for YNL084C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1597	7-benzyloxygramine	16.90	47.5 µM	1.04E-47	calcium & mitochondrial duress	6	2.03E-64
2	SGTC_2399	5884133	16.30	200.0 µM	1.97E-44	cell wall signaling	3	6.02E-60
3	SGTC_2741	miltefosine	15.10	3.0 µM	2.62E-38		2	1.35E-51
4	SGTC_2679	rapamycin	14.50	1.0 nM	1.91E-35		7	1.09E-47
5	SGTC_310	1273-0058	14.30	1.1 µM	1.19E-34	mitochondrial stress	10	1.31E-46
6	SGTC_2007	4011539	12.10	71.8 µM	2.33E-25	cell wall signaling	9	6.11E-34
7	SGTC_663	1486-0733	11.40	13.9 µM	1.03E-22	cell wall signaling	10	2.46E-30
8	SGTC_2582	agaric acid	9.84	100.0 µM	1.95E-17	redox potentiating	66	3.80E-23
9	SGTC_2935	9038016	9.83	30.0 µM	2.05E-17		1	4.07E-23
10	SGTC_2009	4012297	8.08	12.1 µM	2.38E-12	cell wall signaling	14	3.16E-16
11	SGTC_221	trifluoperazine	7.92	10.3 µM	6.25E-12	NEO1-PIK1	86	1.17E-15
12	SGTC_2272	7805186	7.75	180.0 µM	1.68E-11	cell wall signaling	4	4.50E-15
13	SGTC_1635	st007035	7.51	42.4 µM	6.70E-11		103	2.94E-14
14	SGTC_500	h-89	7.44	112.0 µM	1.01E-10	cell wall signaling	6	5.17E-14
15	SGTC_1844	rapamycin	7.30	4.0 nM	2.18E-10		67	1.46E-13
16	SGTC_241	5hr heat shock (37°C) + cantharidin	7.25	155.4 µM	2.84E-10	cell wall signaling	23	2.10E-13
17	SGTC_2666	tannic acid	7.21	100.0 µM	3.61E-10	iron homeostasis	6	2.89E-13
18	SGTC_168	3970-0862	7.15	530.5 µM	4.92E-10		26	4.42E-13
19	SGTC_1750	st081859	6.88	2.4 µM	2.01E-9	endomembrane recycling	90	2.99E-12
20	SGTC_2179	6298986	6.24	200.0 µM	4.87E-8	heme requiring	3	2.25E-10

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.306	1.64E-73	YBL007C	SLA1	hom	Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains
0.305	2.02E-73	YBR260C	RGD1	hom	GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.282	1.38E-62	YNL322C	KRE1	hom	Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.281	9.92E-62	YGR196C	FYV8	hom	Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.269	1.60E-56	YJL095W	BCK1	hom	Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
0.266	1.73E-55	YLR332W	MID2	hom	O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
0.263	2.34E-54	YAL053W	FLC2	hom	Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
0.250	3.79E-49	YAL056C-A_d	YAL056C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.247	9.74E-48	YDL203C	ACK1	hom	Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.236	1.10E-43	YOR035C	SHE4	hom	Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization
0.219	9.82E-38	YMR200W	ROT1	het	Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
0.207	1.16E-33	YJL183W	MNN11	hom	Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
0.204	9.25E-33	YHR030C	SLT2	hom	Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
0.196	1.89E-30	YOR008C	SLG1	hom	Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
0.192	5.31E-29	YGR078C	PAC10	hom	Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding