YNL087W / TCB2

ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL087W / TCB2

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.42|P-value:4.27E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:5.07|P-value:3.88E-7 ID:SGTC_67|Compound:0335-0847|FD-Score:4.00|P-value:4.78E-5 ID:SGTC_88|Compound:0180-0332|FD-Score:3.48|P-value:3.44E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-3.98|P-value:5.85E-5 ID:SGTC_340|Compound:k038-0059|FD-Score:3.41|P-value:4.44E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:3.40|P-value:4.63E-4 ID:SGTC_991|Compound:3996-0057|FD-Score:-3.28|P-value:7.41E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:3.95|P-value:5.96E-5 ID:SGTC_1213|Compound:1315-0166|FD-Score:4.00|P-value:4.85E-5 ID:SGTC_1242|Compound:0402-0103|FD-Score:-4.66|P-value:3.24E-6 ID:SGTC_1250|Compound:0485-0349|FD-Score:-3.20|P-value:9.71E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:3.27|P-value:7.04E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.90|P-value:7.06E-5 ID:SGTC_1594|Compound:piperine|FD-Score:-3.62|P-value:2.31E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.59|P-value:2.58E-4 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:-4.65|P-value:3.28E-6 ID:SGTC_1606|Compound:st000094|FD-Score:3.93|P-value:6.42E-5 ID:SGTC_1609|Compound:st001574|FD-Score:4.48|P-value:6.31E-6 ID:SGTC_1712|Compound:st032288|FD-Score:-3.50|P-value:3.54E-4 ID:SGTC_1758|Compound:st042942|FD-Score:3.25|P-value:7.72E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.34|P-value:5.59E-4 ID:SGTC_1901|Compound:st060216|FD-Score:5.54|P-value:3.40E-8 ID:SGTC_1913|Compound:st060213|FD-Score:-3.74|P-value:1.47E-4 ID:SGTC_1975|Compound:st070967|FD-Score:3.19|P-value:9.25E-4 ID:SGTC_2069|Compound:5228469|FD-Score:4.57|P-value:4.27E-6 ID:SGTC_2077|Compound:5212015|FD-Score:4.02|P-value:4.41E-5 ID:SGTC_2141|Compound:5621638|FD-Score:3.37|P-value:5.09E-4 ID:SGTC_2477|Compound:5967686|FD-Score:3.23|P-value:8.02E-4 ID:SGTC_2587|Compound:piperic acid|FD-Score:3.55|P-value:2.64E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.19|P-value:9.26E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:-3.66|P-value:2.01E-4 ID:SGTC_2831|Compound:7999036|FD-Score:-3.21|P-value:9.53E-4 ID:SGTC_2869|Compound:9037858|FD-Score:4.18|P-value:2.34E-5 ID:SGTC_2942|Compound:9052777|FD-Score:4.51|P-value:5.63E-6 ID:SGTC_2962|Compound:9082311|FD-Score:3.29|P-value:6.67E-4 ID:SGTC_3001|Compound:9076041|FD-Score:-3.23|P-value:8.79E-4 ID:SGTC_3055|Compound:9094636|FD-Score:-3.30|P-value:7.04E-4 ID:SGTC_3095|Compound:9117207|FD-Score:-3.62|P-value:2.26E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.42|P-value:4.27E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:5.07|P-value:3.88E-7 ID:SGTC_67|Compound:0335-0847|FD-Score:4.00|P-value:4.78E-5 ID:SGTC_88|Compound:0180-0332|FD-Score:3.48|P-value:3.44E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-3.98|P-value:5.85E-5 ID:SGTC_340|Compound:k038-0059|FD-Score:3.41|P-value:4.44E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:3.40|P-value:4.63E-4 ID:SGTC_991|Compound:3996-0057|FD-Score:-3.28|P-value:7.41E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:3.95|P-value:5.96E-5 ID:SGTC_1213|Compound:1315-0166|FD-Score:4.00|P-value:4.85E-5 ID:SGTC_1242|Compound:0402-0103|FD-Score:-4.66|P-value:3.24E-6 ID:SGTC_1250|Compound:0485-0349|FD-Score:-3.20|P-value:9.71E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:3.27|P-value:7.04E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.90|P-value:7.06E-5 ID:SGTC_1594|Compound:piperine|FD-Score:-3.62|P-value:2.31E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.59|P-value:2.58E-4 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:-4.65|P-value:3.28E-6 ID:SGTC_1606|Compound:st000094|FD-Score:3.93|P-value:6.42E-5 ID:SGTC_1609|Compound:st001574|FD-Score:4.48|P-value:6.31E-6 ID:SGTC_1712|Compound:st032288|FD-Score:-3.50|P-value:3.54E-4 ID:SGTC_1758|Compound:st042942|FD-Score:3.25|P-value:7.72E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.34|P-value:5.59E-4 ID:SGTC_1901|Compound:st060216|FD-Score:5.54|P-value:3.40E-8 ID:SGTC_1913|Compound:st060213|FD-Score:-3.74|P-value:1.47E-4 ID:SGTC_1975|Compound:st070967|FD-Score:3.19|P-value:9.25E-4 ID:SGTC_2069|Compound:5228469|FD-Score:4.57|P-value:4.27E-6 ID:SGTC_2077|Compound:5212015|FD-Score:4.02|P-value:4.41E-5 ID:SGTC_2141|Compound:5621638|FD-Score:3.37|P-value:5.09E-4 ID:SGTC_2477|Compound:5967686|FD-Score:3.23|P-value:8.02E-4 ID:SGTC_2587|Compound:piperic acid|FD-Score:3.55|P-value:2.64E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.19|P-value:9.26E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:-3.66|P-value:2.01E-4 ID:SGTC_2831|Compound:7999036|FD-Score:-3.21|P-value:9.53E-4 ID:SGTC_2869|Compound:9037858|FD-Score:4.18|P-value:2.34E-5 ID:SGTC_2942|Compound:9052777|FD-Score:4.51|P-value:5.63E-6 ID:SGTC_2962|Compound:9082311|FD-Score:3.29|P-value:6.67E-4 ID:SGTC_3001|Compound:9076041|FD-Score:-3.23|P-value:8.79E-4 ID:SGTC_3055|Compound:9094636|FD-Score:-3.30|P-value:7.04E-4 ID:SGTC_3095|Compound:9117207|FD-Score:-3.62|P-value:2.26E-4

Top fitness defect scores for YNL087W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1901 st060216 5.54 58.6 µM 3.40E-8 9 1.50E-8
2 SGTC_64 000l-0018 5.07 272.1 µM 3.88E-7 ubiquinone biosynthesis & proteasome 106 1.96E-7
3 SGTC_2069 5228469 4.57 200.0 µM 4.27E-6 5 2.45E-6
4 SGTC_2942 9052777 4.51 62.2 µM 5.63E-6 19 3.28E-6
5 SGTC_1609 st001574 4.48 46.4 µM 6.31E-6 5 3.71E-6
6 SGTC_2869 9037858 4.18 32.5 µM 2.34E-5 10 1.47E-5
7 SGTC_2077 5212015 4.02 200.0 µM 4.41E-5 67 2.88E-5
8 SGTC_67 0335-0847 4.00 266.0 µM 4.78E-5 ubiquinone biosynthesis & proteasome 118 3.13E-5
9 SGTC_1213 1315-0166 4.00 115.0 µM 4.85E-5 28 3.18E-5
10 SGTC_999 4022-0066 3.95 22.7 µM 5.96E-5 Golgi 44 3.95E-5
11 SGTC_1606 st000094 3.93 49.2 µM 6.42E-5 60S ribosome export 19 4.27E-5
12 SGTC_1583 clindamycin 3.90 39.6 µM 7.06E-5 mitochondrial processes 14 4.72E-5
13 SGTC_2587 piperic acid 3.55 92.2 µM 2.64E-4 34 1.89E-4
14 SGTC_88 0180-0332 3.48 8.7 µM 3.44E-4 35 2.50E-4
15 SGTC_6 0844-0013 3.42 19.8 µM 4.27E-4 147 3.14E-4
16 SGTC_340 k038-0059 3.41 454.0 µM 4.44E-4 ubiquinone biosynthesis & proteasome 60 3.27E-4
17 SGTC_966 1300-0360 3.40 30.2 µM 4.63E-4 heme requiring 45 3.42E-4
18 SGTC_2141 5621638 3.37 7.7 µM 5.09E-4 tubulin folding & SWR complex 45 3.78E-4
19 SGTC_1873 rapamycin 3.34 2.0 nM 5.59E-4 93 4.17E-4
20 SGTC_2962 9082311 3.29 13.2 µM 6.67E-4 47 5.02E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.160 8.95E-21 YMR110C HFD1 hom Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
0.149 3.33E-18 YLR199C PBA1 hom Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly
0.143 8.80E-17 YBR003W COQ1 hom Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
0.143 8.91E-17 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.134 6.34E-15 YPR146C_d YPR146C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.130 4.63E-14 YJL168C SET2 hom Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
0.129 8.09E-14 YPR024W YME1 hom Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover
0.120 3.49E-12 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.116 1.48E-11 YDR204W COQ4 hom Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.113 5.00E-11 YOR155C ISN1 hom Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.109 2.25E-10 YDL183C YDL183C hom Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene
0.107 4.36E-10 YDR170C SEC7 het Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles
0.107 4.82E-10 YNL105W_d RRT16_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription
0.107 5.67E-10 YOR125C CAT5 hom Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
0.105 1.09E-9 YER087C-A_d YER087C-A_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase