YNL088W / TOP2

Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL088W / TOP2

Click on Significant Values for Screen Details ID:SGTC_116|Compound:0155-0182|FD-Score:3.53|P-value:1.10E-4 ID:SGTC_210|Compound:3551-0445|FD-Score:-2.94|P-value:9.63E-4 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:-3.26|P-value:2.90E-4 ID:SGTC_254|Compound:nsc-64875|FD-Score:-4.78|P-value:2.29E-7 ID:SGTC_260|Compound:nsc-65238|FD-Score:-4.33|P-value:2.47E-6 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:-2.98|P-value:8.27E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:4.55|P-value:9.14E-7 ID:SGTC_270|Compound:tamoxifen|FD-Score:-3.33|P-value:2.21E-4 ID:SGTC_295|Compound:k015-0027|FD-Score:3.17|P-value:4.40E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:3.08|P-value:6.34E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.15|P-value:4.86E-4 ID:SGTC_597|Compound:k018-0003|FD-Score:-3.11|P-value:5.09E-4 ID:SGTC_658|Compound:3253-0951|FD-Score:3.66|P-value:6.20E-5 ID:SGTC_748|Compound:0136-0228|FD-Score:-2.93|P-value:9.99E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:4.25|P-value:4.12E-6 ID:SGTC_803|Compound:4488-1585|FD-Score:3.83|P-value:3.01E-5 ID:SGTC_1024|Compound:4507-0395|FD-Score:4.51|P-value:1.12E-6 ID:SGTC_1082|Compound:camptothecin|FD-Score:-4.10|P-value:7.56E-6 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.11|P-value:5.68E-4 ID:SGTC_1226|Compound:3909-7979|FD-Score:-2.94|P-value:9.43E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.73|P-value:4.68E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.13|P-value:5.14E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.87|P-value:2.50E-5 ID:SGTC_1483|Compound:3453-0561|FD-Score:5.43|P-value:6.11E-9 ID:SGTC_1494|Compound:4358-1428|FD-Score:3.36|P-value:2.14E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:-3.01|P-value:7.48E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:3.41|P-value:1.77E-4 ID:SGTC_1523|Compound:st012844|FD-Score:3.01|P-value:8.10E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:4.32|P-value:2.93E-6 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:4.39|P-value:2.08E-6 ID:SGTC_1707|Compound:st032323|FD-Score:-5.63|P-value:1.49E-9 ID:SGTC_1770|Compound:st043884|FD-Score:3.68|P-value:5.66E-5 ID:SGTC_1772|Compound:st048439|FD-Score:3.00|P-value:8.41E-4 ID:SGTC_1830|Compound:st055982|FD-Score:3.01|P-value:8.11E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.05|P-value:6.98E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:3.11|P-value:5.54E-4 ID:SGTC_1923|Compound:st058458|FD-Score:2.98|P-value:9.01E-4 ID:SGTC_1930|Compound:st074634|FD-Score:-4.05|P-value:9.93E-6 ID:SGTC_2124|Compound:5246320|FD-Score:-3.07|P-value:5.99E-4 ID:SGTC_2310|Compound:7668677|FD-Score:2.98|P-value:8.97E-4 ID:SGTC_2381|Compound:9035592|FD-Score:-3.53|P-value:9.94E-5 ID:SGTC_2434|Compound:5670830|FD-Score:3.08|P-value:6.26E-4 ID:SGTC_2486|Compound:5283202|FD-Score:3.23|P-value:3.52E-4 ID:SGTC_2488|Compound:5268135|FD-Score:3.14|P-value:5.05E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.99|P-value:1.45E-5 ID:SGTC_2525|Compound:harmine|FD-Score:3.13|P-value:5.23E-4 ID:SGTC_2657|Compound:nystatin|FD-Score:3.16|P-value:4.60E-4 ID:SGTC_2882|Compound:9058044|FD-Score:-3.78|P-value:3.40E-5 ID:SGTC_2892|Compound:9047358|FD-Score:-3.64|P-value:6.11E-5 ID:SGTC_2897|Compound:9048598|FD-Score:-3.66|P-value:5.69E-5 ID:SGTC_2925|Compound:9010536|FD-Score:-6.81|P-value:3.57E-13 ID:SGTC_3075|Compound:9116269|FD-Score:3.15|P-value:4.83E-4 ID:SGTC_3083|Compound:9117465|FD-Score:5.03|P-value:6.66E-8 ID:SGTC_3085|Compound:9117376|FD-Score:-3.92|P-value:1.75E-5 ID:SGTC_3090|Compound:9117696|FD-Score:3.82|P-value:3.14E-5 ID:SGTC_3091|Compound:9116372|FD-Score:5.48|P-value:4.40E-9 ID:SGTC_3092|Compound:9117248|FD-Score:-3.73|P-value:4.14E-5 ID:SGTC_3094|Compound:9117742|FD-Score:3.79|P-value:3.55E-5 ID:SGTC_3095|Compound:9117207|FD-Score:3.70|P-value:5.16E-5 ID:SGTC_3113|Compound:9123498|FD-Score:3.50|P-value:1.24E-4 ID:SGTC_3204|Compound:9114183|FD-Score:2.99|P-value:8.59E-4 ID:SGTC_3247|Compound:9135149|FD-Score:-4.73|P-value:3.09E-7 ID:SGTC_3304|Compound:9125678|FD-Score:3.38|P-value:2.01E-4 ID:SGTC_3319|Compound:9138450|FD-Score:3.12|P-value:5.40E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:3.53|P-value:1.10E-4 ID:SGTC_210|Compound:3551-0445|FD-Score:-2.94|P-value:9.63E-4 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:-3.26|P-value:2.90E-4 ID:SGTC_254|Compound:nsc-64875|FD-Score:-4.78|P-value:2.29E-7 ID:SGTC_260|Compound:nsc-65238|FD-Score:-4.33|P-value:2.47E-6 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:-2.98|P-value:8.27E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:4.55|P-value:9.14E-7 ID:SGTC_270|Compound:tamoxifen|FD-Score:-3.33|P-value:2.21E-4 ID:SGTC_295|Compound:k015-0027|FD-Score:3.17|P-value:4.40E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:3.08|P-value:6.34E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.15|P-value:4.86E-4 ID:SGTC_597|Compound:k018-0003|FD-Score:-3.11|P-value:5.09E-4 ID:SGTC_658|Compound:3253-0951|FD-Score:3.66|P-value:6.20E-5 ID:SGTC_748|Compound:0136-0228|FD-Score:-2.93|P-value:9.99E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:4.25|P-value:4.12E-6 ID:SGTC_803|Compound:4488-1585|FD-Score:3.83|P-value:3.01E-5 ID:SGTC_1024|Compound:4507-0395|FD-Score:4.51|P-value:1.12E-6 ID:SGTC_1082|Compound:camptothecin|FD-Score:-4.10|P-value:7.56E-6 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.11|P-value:5.68E-4 ID:SGTC_1226|Compound:3909-7979|FD-Score:-2.94|P-value:9.43E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.73|P-value:4.68E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:3.13|P-value:5.14E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.87|P-value:2.50E-5 ID:SGTC_1483|Compound:3453-0561|FD-Score:5.43|P-value:6.11E-9 ID:SGTC_1494|Compound:4358-1428|FD-Score:3.36|P-value:2.14E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:-3.01|P-value:7.48E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:3.41|P-value:1.77E-4 ID:SGTC_1523|Compound:st012844|FD-Score:3.01|P-value:8.10E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:4.32|P-value:2.93E-6 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:4.39|P-value:2.08E-6 ID:SGTC_1707|Compound:st032323|FD-Score:-5.63|P-value:1.49E-9 ID:SGTC_1770|Compound:st043884|FD-Score:3.68|P-value:5.66E-5 ID:SGTC_1772|Compound:st048439|FD-Score:3.00|P-value:8.41E-4 ID:SGTC_1830|Compound:st055982|FD-Score:3.01|P-value:8.11E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.05|P-value:6.98E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:3.11|P-value:5.54E-4 ID:SGTC_1923|Compound:st058458|FD-Score:2.98|P-value:9.01E-4 ID:SGTC_1930|Compound:st074634|FD-Score:-4.05|P-value:9.93E-6 ID:SGTC_2124|Compound:5246320|FD-Score:-3.07|P-value:5.99E-4 ID:SGTC_2310|Compound:7668677|FD-Score:2.98|P-value:8.97E-4 ID:SGTC_2381|Compound:9035592|FD-Score:-3.53|P-value:9.94E-5 ID:SGTC_2434|Compound:5670830|FD-Score:3.08|P-value:6.26E-4 ID:SGTC_2486|Compound:5283202|FD-Score:3.23|P-value:3.52E-4 ID:SGTC_2488|Compound:5268135|FD-Score:3.14|P-value:5.05E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.99|P-value:1.45E-5 ID:SGTC_2525|Compound:harmine|FD-Score:3.13|P-value:5.23E-4 ID:SGTC_2657|Compound:nystatin|FD-Score:3.16|P-value:4.60E-4 ID:SGTC_2882|Compound:9058044|FD-Score:-3.78|P-value:3.40E-5 ID:SGTC_2892|Compound:9047358|FD-Score:-3.64|P-value:6.11E-5 ID:SGTC_2897|Compound:9048598|FD-Score:-3.66|P-value:5.69E-5 ID:SGTC_2925|Compound:9010536|FD-Score:-6.81|P-value:3.57E-13 ID:SGTC_3075|Compound:9116269|FD-Score:3.15|P-value:4.83E-4 ID:SGTC_3083|Compound:9117465|FD-Score:5.03|P-value:6.66E-8 ID:SGTC_3085|Compound:9117376|FD-Score:-3.92|P-value:1.75E-5 ID:SGTC_3090|Compound:9117696|FD-Score:3.82|P-value:3.14E-5 ID:SGTC_3091|Compound:9116372|FD-Score:5.48|P-value:4.40E-9 ID:SGTC_3092|Compound:9117248|FD-Score:-3.73|P-value:4.14E-5 ID:SGTC_3094|Compound:9117742|FD-Score:3.79|P-value:3.55E-5 ID:SGTC_3095|Compound:9117207|FD-Score:3.70|P-value:5.16E-5 ID:SGTC_3113|Compound:9123498|FD-Score:3.50|P-value:1.24E-4 ID:SGTC_3204|Compound:9114183|FD-Score:2.99|P-value:8.59E-4 ID:SGTC_3247|Compound:9135149|FD-Score:-4.73|P-value:3.09E-7 ID:SGTC_3304|Compound:9125678|FD-Score:3.38|P-value:2.01E-4 ID:SGTC_3319|Compound:9138450|FD-Score:3.12|P-value:5.40E-4

Top fitness defect scores for YNL088W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3091 9116372 5.48 49.5 µM 4.40E-9 3 2.10E-8 0.05
2 SGTC_1483 3453-0561 5.43 55.9 µM 6.11E-9 2 2.84E-8 1.38
3 SGTC_3083 9117465 5.03 49.5 µM 6.66E-8 2 2.49E-7 0.56
4 SGTC_266 formosanin C 4.55 406.0 nM 9.14E-7 sphingolipid biosynthesis & PDR1 19 2.69E-6 0.15
5 SGTC_1024 4507-0395 4.51 26.0 µM 1.12E-6 TSC3-RPN4 8 3.23E-6 0.01
6 SGTC_1579 chlormadinone acetate 4.39 49.4 µM 2.08E-6 4 5.68E-6 0.13
7 SGTC_1529 apigenin 4.32 74.0 µM 2.93E-6 2 7.76E-6 0.14
8 SGTC_798 1287-0182 4.25 476.0 µM 4.12E-6 1 1.06E-5 0.45
9 SGTC_2520 obtusaquinone 3.99 4.7 µM 1.45E-5 4 3.33E-5 0.08
10 SGTC_1360 1598-0022 3.87 39.8 µM 2.50E-5 ERG2 11 5.49E-5 0.17
11 SGTC_803 4488-1585 3.83 147.0 µM 3.01E-5 4 6.50E-5 0.02
12 SGTC_3090 9117696 3.82 49.5 µM 3.14E-5 2 6.74E-5 0.02
13 SGTC_3094 9117742 3.79 49.5 µM 3.55E-5 4 7.54E-5 0.02
14 SGTC_1237 0330-0133 3.73 46.1 µM 4.68E-5 ergosterol biosynthesis 12 9.71E-5 0.10
15 SGTC_3095 9117207 3.70 49.5 µM 5.16E-5 2 1.06E-4 0.19
16 SGTC_1770 st043884 3.68 66.3 µM 5.66E-5 5 1.15E-4 0.10
17 SGTC_658 3253-0951 3.66 102.0 µM 6.20E-5 calcium & mitochondrial duress 8 1.25E-4 0.14
18 SGTC_116 0155-0182 3.53 31.8 µM 1.10E-4 mitochondrial response to ROS 7 2.11E-4 0.04
19 SGTC_3113 9123498 3.50 49.5 µM 1.24E-4 10 2.35E-4 0.03
20 SGTC_1514 1189-1591 3.41 158.0 µM 1.77E-4 10 3.26E-4 0.25

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.104 1.73E-9 YDL070W BDF2 hom Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress
0.104 1.77E-9 YPR154W PIN3 hom Protein that induces appearance of [PIN+] prion when overproduced
0.093 5.85E-8 YNL244C SUI1 het Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase
0.092 1.00E-7 YPL057C SUR1 hom Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
0.089 2.17E-7 YKR040C_d YKR040C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W
0.089 2.21E-7 YEL057C_p YEL057C_p hom Protein of unknown function involved in telomere maintenance; target of UME6 regulation
0.086 7.36E-7 YHR068W DYS1 het Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric
0.084 1.23E-6 YGL155W CDC43 het Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
0.083 1.68E-6 YKL019W RAM2 het Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor
0.082 1.94E-6 YMR102C_p YMR102C_p hom Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene
0.079 4.12E-6 YMR169C ALD3 hom Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose
0.079 4.16E-6 YGR283C YGR283C hom Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus
0.079 4.32E-6 YDR072C IPT1 hom Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin
0.077 7.89E-6 YLR076C_d YLR076C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
0.076 9.63E-6 YMR128W ECM16 het Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis