HIPHOP chemogenomics database

YNL089C_d

Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins

Zygosity: Homozygous strain

fixedexpanded
Profile for YNL089C / YNL089C

Click on Significant Values for Screen Details

Top fitness defect scores for YNL089C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_663	1486-0733	7.93	13.9 µM	1.51E-16	cell wall signaling	16	1.11E-15
2	SGTC_1448	4255-0083	7.20	152.0 µM	6.07E-14	ERG2	2	3.09E-13
3	SGTC_367	0335-0881	5.52	7.1 µM	6.58E-9	ubiquinone biosynthesis & proteasome	30	1.66E-8
4	SGTC_684	1140-0066	5.37	328.0 µM	1.63E-8	Golgi	5	3.91E-8
5	SGTC_360	0957-0399	4.37	1.0 µM	3.54E-6	cell wall	33	6.19E-6
6	SGTC_3337	9144342	4.27	33.5 µM	5.72E-6		21	9.71E-6
7	SGTC_702	1488-0190	4.24	94.0 µM	6.48E-6	amide catabolism	11	1.09E-5
8	SGTC_3345	9148410	4.21	3.6 µM	7.68E-6		5	1.28E-5
9	SGTC_228	caffeine	3.86	993.1 µM	3.67E-5		10	5.61E-5
10	SGTC_2958	9077254	3.84	16.7 µM	4.02E-5		35	6.11E-5
11	SGTC_661	1304-0007	3.84	33.6 µM	4.08E-5		25	6.20E-5
12	SGTC_533	1222-0222	3.77	6.7 µM	5.47E-5	PDR1	36	8.18E-5
13	SGTC_1405	3909-7894	3.75	12.0 µM	5.82E-5	60S ribosome export	14	8.67E-5
14	SGTC_549	1661-1316	3.64	519.0 µM	9.23E-5		24	1.34E-4
15	SGTC_2954	9074818	3.61	52.7 µM	1.06E-4		46	1.53E-4
16	SGTC_1452	4194-0072	3.61	190.0 µM	1.07E-4		21	1.54E-4
17	SGTC_1469	k284-2957	3.61	126.0 µM	1.08E-4		14	1.56E-4
18	SGTC_2078	5212016	3.60	200.0 µM	1.09E-4	60S ribosome export	23	1.56E-4
19	SGTC_2749	bexarotene	3.54	57.4 µM	1.40E-4		38	1.98E-4
20	SGTC_2577	2',4'-dihydroxy-4-methoxychalcone	3.49	15.3 µM	1.70E-4		53	2.39E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.197	1.42E-30	YDL203C	ACK1	hom	Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.176	1.00E-24	YLR331C_d	JIP3_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.167	2.67E-22	YKR042W	UTH1	hom	Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
0.166	3.80E-22	YLR332W	MID2	hom	O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
0.165	6.27E-22	YGR196C	FYV8	hom	Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.160	1.11E-20	YBR260C	RGD1	hom	GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.160	1.95E-20	YHR030C	SLT2	hom	Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
0.135	4.31E-15	YOR231W	MKK1	hom	MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.127	1.69E-13	YLL043W	FPS1	hom	Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.123	7.91E-13	YJL095W	BCK1	hom	Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
0.116	1.62E-11	YMR200W	ROT1	het	Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation
0.107	4.61E-10	YBL061C	SKT5	hom	Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
0.107	5.04E-10	YAL056C-A_d	YAL056C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.106	7.06E-10	YPR165W	RHO1	het	GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
0.106	7.55E-10	YBR142W	MAK5	het	Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits